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Image Acquisition with the AxioCam MRc5
2.8 Background Information
Gamma setting
This setting is required to compensate for the inherently non-linear behavior
of the image tube ("gamma"). It also influences the quality of the color
display.
You can get the best display of colored objects, if the gamma setting is in the
range of approx. 0,45.
Many video cameras have built-in gamma compensation, as they are intended
for use on video monitors.
Linearity of AxioCam MRc5
All versions of AxioCam MRc5 have been designed to determine linear,
quantitative measurement data, as such data is frequently used for tasks in
the field of image analysis. Consequently, internal gamma compensation has
been deliberately omitted from the AxioCam MRc5.
Fluorescence images
Due to its high sensitivity and the long exposure times that are possible, the
AxioCam MRc5 can also be used for fluorescence images in the field of
microscopy. However, please bear in mind the following points:
Reduced resolution: the color sensor uses a so-called Bayer color filter mask
on the pixels in order to produce color. Consequently, pixels with a red filter
are sensitive only to red spectra, pixels with a blue filter only to blue spectra,
and pixels with a green filter only to green spectra. These filters are distributed
in such a way that half of all the pixels are covered with green filters. Red and
blue filters each cover a quarter of the pixels.
AxioCam MRc5 Installation + Reference
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