Summary of Contents for Hanna Instruments HI 83215
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99 Washington Street Melrose, MA 02176 Phone 781-665-1400 Toll Free 1-800-517-8431 Visit us at www.TestEquipmentDepot.com Instruction Manual HI 83215 Grow Master Basic for Nutrient Analyses w w w . h a n n a i n s t . c o m...
Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for the correct use of the instrument. TABLE OF CONTENTS PRELIMINARY EXAMINATION .............................. 3 GENERAL DESCRIPTION ..............................
The amount of reagent is precisely dosed to ensure maximum reproducibility. HI 83215 bench photometer can be connected to a PC via an USB cable. The optional HI 92000 Windows Compatible Software helps users manage all their results.
ABBREVIATIONS °C: degree Celsius °F: degree Fahrenheit μg/L: micrograms per liter (ppb) mg/L: milligrams per liter (ppm) g/L: grams per liter (ppt) milliliter high range medium range low range SPECIFICATIONS Light Life Life of the instrument Light Detector Silicon Photocell Environment 0 to 50°C (32 to 122°F);...
The optical system of HI 83215 is based on special subminiature tungsten lamps and narrow-band interference filters to guarantee both high performance and reliable results.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual measurement is performed. The cuvette has a very important role because it is an optical element and thus requires particular attention. It is important that both the measurement and the calibration (zeroing) cuvette are optically identical to provide the same measurement conditions.
1) Open Cuvette Lid 2) Indexing mark 3) Cuvette point 4) Liquid Crystal Display (LCD) 5) Splash proof keypad 6) ON/OFF power switch 7) Power input connector 8) USB connector KEYPAD DESCRIPTION The keypad contains 8 direct keys and 3 functional keys with the following functions: Press to perform the function displayed above it on the LCD.
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(b) pull the plunger up until the lower edge of the seal is exactly on the 0.0 mL mark. (c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them. Then, keeping the syringe in vertical position above the cuvette, push the plunger down into the syringe until the lower edge of the seal is exactly on the 0.5 mL mark.
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USING CUVETTES • Proper mixing is very important for reproducibility of the measurements. The right way of mixing a cuvette is specified for each method in the related chapter. (a) invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical position. Turn the cuvette upside-down and wait for all of the solution to flow to the cap end, then return the cuvette to the upright vertical position and wait for all of the solution to flow to the cuvette bottom.
• In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied HDPE plastic stopper and then the black cap. • Whenever the cuvette is placed into the measurement cell, it must be dry outside, and free of fingerprints, oil or dirt.
METHOD REFERENCE TABLE Method Method Page Method Method Page description description Ammonia HR Phosphorus HR Ammonia MR Phosphorus MR Phosphorus LR Ammonia LR Nitrate HR Potassium HR Nitrate MR Potassium MR Nitrate LR Potassium LR OPERATIONAL GUIDE POWER CONNECTION AND BATTERY MANAGEMENT The meter can be powered from an AC/DC adapter (included) or from the built-in rechargeable battery.
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METHOD SELECTION • Turn the instrument ON via the ON/OFF power switch. • The meter will perform an autodiagnostic test. During this test, the Hanna Instrument logo will appear on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display. •...
CHEMICAL FORM Chemical form conversion factors are pre-programmed into the instrument and are method specific. In order to view the displayed result in the desired chemical form press to access the second level functions and then press the Chem Frm key to toggle between the available chemical forms for the selected method. S E T U P S E T U P S E T U P...
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Date / Time This option is used to set the instrument’s date and time. Press the Modify key to change the date/time. Press the functional keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the keys to change the value.
H E L P M O D E HELP MODE HI 83215 offers an interactive contextual help mode that assists the user at any time. To access the help screens press HELP. The instrument will display additional information related to the current screen.
I N T R O D U C T I O N I N T R O D U C T I O N I N T R O D U C T I O N I N T R O D U C T I O N I N T R O D U C T I O N THE ROLE OF NUTRIENTS IN THE GROWTH AND PRODUCTION OF PLANTS The three elements that are mostly needed by the plants are nitrogen (N), phosphorus (P) and potassium...
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PHOSPHORUS Phosphorus (P) has an important role in many fundamental biochemical and physiological processes. Plants take up phosphorus in the form of phosphate ion (PO ¯). PRESENT IN DNA and RNA, ATP, ADP ACTION • stimulates the roots growth • stimulates blooming •...
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IRRIGATION WATER (LOW RANGE) In agricultural areas it is quite common to find altered values in the chemical composition of irrigation waters. The problem concerns mostly the high nitrate concentration, usually determined by excessive fertilization or irrational liquid manure spreading. The analysis of irrigation waters allows us to find out which are the substances present in major or minor quantity and to organize an advantageous fertilization plan.
SAMPLE PREPARATION SAMPLE PREPARATION PROCEDURE Note: If the water sample to be analyzed is very turbid, let it stand in a beaker for a while until most of the solid particles have settled. Then, use the pipette to transfer the supernatant solution to the other beaker and prepare the samples as described below.
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• Fold a filter disc twice as shown in the figure. Separate one side from the other three to form a cone. Insert the folded filter disc in the funnel. • Filter the treated sample into an empty beaker. The sample is now ready. Note: Filter at least 40 mL of solution if all the four methods will be tested.
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• Replace the cap and shake gently for at least 2 minutes. • Open the upper part of the Demineralizer Bottle cap and gently squirt the demineralized water into the cylinder, up to the 100 mL mark. Note: The ion exchange resin contained in the Demineralizer Bottle is provided with an indicator substance.
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Note: To measure exactly 5 mL of sample with the syringe, push the plunger completely into the syringe and insert the tip into the sample. Pull the plunger out until the lower edge of the seal is on the 5 mL mark of the syringe. •...
AMMONIA HIGH RANGE SPECIFICATIONS Range 0 to 100 mg/L Resolution 1 mg/L Accuracy ±1 mg/L ±4% of reading at 25 °C Typical EMC ±1 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92 , Method Nessler method.
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• Remove the cuvette. • Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis).
AMMONIA MEDIUM RANGE SPECIFICATIONS Range 0.0 to 50.0 mg/L Resolution 0.5 mg/L Accuracy ±0.5 mg/L ±4% of reading at 25 °C Typical EMC ±0.5 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92 , Method Nessler method.
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• Remove the cuvette. • Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis).
AMMONIA LOW RANGE SPECIFICATIONS Range 0.0 to 10.0 mg/L Resolution 0.1 mg/L Accuracy ±0.1 mg/L ±4% of reading at 25 °C Typical EMC ±0.1 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92 , Method Nessler method.
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• Remove the cuvette. • Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis).
NITRATE HIGH RANGE SPECIFICATIONS Range 0 to 300 mg/L Resolution 5 mg/L Accuracy ±10 mg/L ±8% of reading at 25 °C Typical EMC ±5 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the cadmium reduction method. The reaction between nitrate-nitrogen and the reagent causes an amber tint in the sample.
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• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement.
NITRATE MEDIUM RANGE SPECIFICATIONS Range 0 to 150 mg/L Resolution 2.5 mg/L Accuracy ±5 mg/L ±8% of reading at 25 °C Typical EMC ±2.5 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the cadmium reduction method. The reaction between nitrate-nitrogen and the reagent causes an amber tint in the sample.
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• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement.
NITRATE LOW RANGE SPECIFICATIONS Range 0.0 to 30.0 mg/L Resolution 0.5 mg/L Accuracy ±1.0 mg/L ±8% of reading at 25 °C Typical EMC ±0.5 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the cadmium reduction method. The reaction between nitrate-nitrogen and the reagent causes an amber tint in the sample.
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• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement.
PHOSPHORUS HIGH RANGE SPECIFICATIONS Range 0 to 100 mg/L Resolution 1 mg/L Accuracy ±5 mg/L ±4% of reading at 25 °C Typical EMC Dev. ±1 mg/L Light Source Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, Method edition, Amino Acid method.
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• Add the content of one packet of HI 93706B-0 Phosphorus Reagent B (Amino Acid) to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read.
PHOSPHORUS MEDIUM RANGE SPECIFICATIONS Range 0.0 to 50.0 mg/L Resolution 0.5 mg/L Accuracy ±2.5 mg/L ±4% of reading at 25 °C Typical EMC Dev. ±0.5 mg/L Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, edition, Amino Acid method.
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• Add the content of one packet of HI 93706B-0 Phosphorus Reagent B (Amino Acid) to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read.
PHOSPHORUS LOW RANGE SPECIFICATIONS Range 0.0 to 10.0 mg/L Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±4% of reading at 25 °C Typical EMC Dev. ±0.1 mg/L Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, edition, Amino Acid method.
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• Add the content of one packet of HI 93706B-0 Phosphorus Reagent B (Amino Acid) to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read.
POTASSIUM HIGH RANGE SPECIFICATIONS Range 20 to 200 mg/L Resolution 5 mg/L Accuracy ±30 mg/L ±7% of reading at 25 °C Typical EMC ±5 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 610 nm Method Adaptation of the Turbidimetric Tetraphenylborate method. The reaction between Potassium and reagents causes turbidity in the sample.
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• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down.
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INTERFERENCES Interferences may be caused by: Ammonium above 10 ppm Calcium above 10000 ppm as CaCO Chloride above 12000 ppm Magnesium above 8000 ppm as CaCO Sodium above 8000 ppm POTASSIUM ULTRA HIGH RANGE For samples containing more than 200 ppm of Potassium: follow the sample preparation procedure described at page 21 for NUTRIENTS SOLUTION (HR).
POTASSIUM MEDIUM RANGE SPECIFICATIONS Range 10 to 100 mg/L Resolution 2.5 mg/L Accuracy ±15 mg/L ±7% of reading at 25 °C Typical EMC ±2.5 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 610 nm Method Adaptation of the Turbidimetric Tetraphenylborate method. The reaction between Potassium and reagents causes turbidity in the sample.
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• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down.
POTASSIUM LOW RANGE SPECIFICATIONS Range 0.0 to 20.0 mg/L Resolution 0.5 mg/L Accuracy ±3.0 mg/L ±7% of reading at 25 °C ±0.5 mg/L Typical EMC Deviation Light Source Tungsten lamp with narrow band interference filter @ 610 nm Method Adaptation of the Turbidimetric Tetraphenylborate method. The reaction between Potassium and reagents causes turbidity in the sample.
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• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down.
ERRORS AND WARNINGS The instrument shows clear warning messages when erroneous conditions appear and when measured values are outside the expected range. These messages are described bellow. No Light: The light source is not functioning properly. Light Leak: There is an excess amount of ambient light reaching the detector.
DATA MANAGEMENT The analyzed data can be managed using Hanna’s product HI92000, Windows Compatible Software. ® STANDARD METHODS Description Range Method Ammonia HR 0 to 100 mg/L Nessler Ammonia MR 0.0 to 50.0 mg/L Nessler Ammonia LR 0.0 to 10.0 mg/L Nessler Nitrate HR 0 to 300 mg/L...
ACCESSORIES REAGENT SETS HI 93706-01 100 phosphorus tests HI 93706-03 300 phosphorus tests HI 93715-01 100 ammonia tests HI 93715-03 300 ammonia tests HI 93728-01 100 nitrate tests HI 93728-03 300 nitrate tests HI 93750-01 100 potassium tests HI 93750-03 300 potassium tests OTHER ACCESSORIES HI 731318 cloth for wiping cuvettes (4 pcs)
WARRANTY All Hanna Instruments meters are warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact your dealer.
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