Page 1 Vanquish Charged Aerosol Detectors VF-D21, VH-D21 Operating Manual 4820.8501-EN Version 1.0 • June 2025 *4820.8501-EN-1.0*...
Page 3 Contacting us Contacting us You can find information on all of the different support services of Thermo Fisher Scientific by navigating the following website: https://www.thermofisher.com/us/en/home/technical-resources/ contact-us.html Charged Aerosol Detectors (VF-D21, VH-D21) Page 3 Operating Manual...
Page 4 Contacting us Page 4 Charged Aerosol Detectors (VF-D21, VH-D21) Operating Manual...
Page 5 Contents Contents 1 Using this manual ............... 11 About this manual.................... 12 Conventions ...................... 13 1.2.1 Conventions for safety messages............. 13 1.2.2 Special notices.................. 13 1.2.3 Typographical conventions .............. 14 Access documentation.................. 15 Reference documentation ................... 16 2 Safety .................. 19 Safety symbols and signal words .................
Page 6 Contents 3 Device overview ................. 33 Detector features.................... 34 Detector configurations .................. 35 Operating principle .................... 40 Interior detector components ................ 42 3.4.1 Diverter valve ................... 43 3.4.2 Nebulizer .................... 44 3.4.3 Gas filter assembly ................... 44 3.4.4 Waste port .................... 45 Additional components for external configurations ..........
Page 7 Contents Setting up the hardware .................. 70 5.5.1 Moving the system stack (unlocking/locking) .......... 70 5.5.2 Remove and install the carrying handles .......... 72 5.5.3 Arrangement in the integrated Vanquish LC system configuration .. 73 5.5.4 Connecting the device................ 75 5.5.5 Connect the gas inlet and exhaust tubes ..........
Page 8 Contents Optimize the performance of the device............ 146 6.8.1 General guidelines.................. 146 6.8.2 Overview of optimization parameters ........... 147 6.8.3 Data collection rate and filter constant .......... 147 6.8.4 Signal channels.................. 148 6.8.5 Power value.................... 149 6.8.6 Evaporation tube temperature .............. 150 6.8.7 Temperature coupling mode..............
Page 9 Contents 7.14 Replace the solvent rack .................. 202 7.15 Transport or ship the device ................ 204 7.15.1 Prepare the device for transport............ 204 7.15.2 Transport the device to a new location .......... 205 7.15.3 Dismount the stack of an external configuration........ 206 7.15.4 Ship the detector or parts of an external configuration ......
Page 10 Contents 11 Legal notices................ 247 11.1 Copyright and trademarks ................. 248 11.2 Revision history.................... 249 12 Appendix.................. 251 12.1 Digital I/O ports.................... 252 12.1.1 Digital I/O port 1 pinout ................. 252 12.1.2 Digital I/O port 2 pinout ................. 253 12.1.3 Connect the digital I/O ports.............. 255 Index ..................
Page 11 • Using this manual 1 Using this manual This chapter provides information about this manual, the conventions used throughout the manual, and the reference documentation that is available in addition to this manual. Charged Aerosol Detectors (VF-D21, VH-D21) Page 11 Operating Manual...
Page 12 • Using this manual About this manual This manual describes the functional features and operating principle of your Vanquish device and provides instructions for installation, set up, start up, shut down, operation, maintenance and troubleshooting. This manual also contains safety messages, precautionary statements, and special notices.
Page 13 • Using this manual Conventions This section describes the conventions that are used throughout this manual. 1.2.1 Conventions for safety messages The safety messages and precautionary statements in this manual appear as follows: • Safety messages or precautionary statements that apply to the entire manual and all procedures in this manual are grouped in the Safety chapter.
Page 14 • Using this manual 1.2.3 Typographical conventions These typographical conventions apply to the descriptions in this manual: Data input and output The following appears in bold type: • Input that you enter by the keyboard or that you select with the mouse •...
Page 15 Visit the Help Portal In addition, documentation (including software documentation) is maintained in the Thermo Scientific Chromatography and Mass Spectrometry Help portal. To access the Help portal, go to docs.thermofisher.com. TIP With a Thermo Fisher Scientific account, you can use more features of the Help portal, such as bookmarking documents, saving searches, and compiling documents into collections for easy reference.
Page 16 Instrument Installation Qualification Operating Instructions • Vanquish User Interface Installation Guide • Charged Aerosol Detector Third-Party Kit Installation Guide Software documentation Software documentation for the Thermo Scientific Dionex Chromeleon 7 Chromatography Data System includes the following: • Chromeleon™ Help and documents The Chromeleon Help provides extensive information and comprehensive reference material for all aspects of the software.
Page 17 • Using this manual Third-party documentation Refer also to the user documentation provided by the manufacturers of third-party components and materials, for example, Safety Data Sheets (SDSs). Third-party documentation includes the user documentation for the gas supply, as applicable: • User documentation for the nitrogen generator •...
Page 18 • Using this manual Page 18 Charged Aerosol Detectors (VF-D21, VH-D21) Operating Manual...
Page 19 • Safety 2 Safety This chapter provides general and specific safety information and informs about the intended use of the device. Charged Aerosol Detectors (VF-D21, VH-D21) Page 19 Operating Manual...
Page 20 • Safety Safety symbols and signal words 2.1.1 Safety symbols and signal words in this manual This manual contains safety messages to prevent injury of the persons using the device. The safety symbols and signal words in this manual include the following: Always be aware of the safety information.
Page 21 • Safety 2.1.3 Safety symbols on the device The table lists the safety symbols that appear on the device or on labels affixed to the device. Follow the safety notices in this manual to prevent the risk of operator injury or damage to the device. Symbol Description Indicates a potential hazard.
Page 22 • Safety Intended use The device is intended to be part of the Vanquish system. The intended use of the Vanquish system is to analyze mixtures of compounds in sample solutions. The device is for use by qualified personnel and in laboratory environment only.
Page 23 • Safety Safety precautions 2.3.1 General safety information All users must observe the general safety information presented in this section and all specific safety messages and precautionary statements elsewhere in this manual during all phases of installation, operation, troubleshooting, maintenance, shutdown, and transport of the device. If the device is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the device could be impaired.
Page 24 • Safety Installation Installation Only skilled personnel are permitted to install the device and to establish the electrical connections according to the appropriate regulations. • Thermo Fisher Scientific recommends always having service personnel certified by Thermo Fisher Scientific perform the installation (for brevity, referred to as Thermo Fisher Scientific service engineer).
Page 25 • Safety Protective eyewear To prevent liquids from striking your eyes, put on appropriate protective eyewear, such as safety glasses with side shields. If there is a risk of splashing liquids, put on goggles. Gloves To protect you from harmful liquids and avoid personal injury during maintenance or service, put on appropriate protective gloves.
Page 26 • Safety 2.3.5 General residual hazards Pay attention to the following general residual hazards when working with the device: WARNING—Hazardous substances Solvents, mobile phases, samples, and reagents might contain toxic, carcinogenic, mutagenic, infectious, or otherwise harmful substances. The handling of these substances can pose health and safety risks. •...
Page 27 • Safety WARNING—Self-ignition of solvents Solvents with a self-ignition temperature below 150 °C might ignite when in contact with a hot surface (for example, due to leakage in the chromatography system). Avoid the use of these solvents. WARNING—Hazardous vapors Mobile phases and samples might contain volatile or flammable solvents.
Page 28 • Safety CAUTION—Sparking due to electrostatic discharge Liquid flowing through capillaries can generate static electricity. This effect is particularly present with insulating capillaries and non- conductive solvents (for example, pure acetonitrile). Discharge of electrostatic energy might lead to sparking, which could constitute a fire hazard.
Page 29 • Safety Solvent and additive information 2.4.1 General compatibility To protect optimal functionality of the Vanquish system, observe these recommendations on the use of solvents and additives: • By default, the system must be used with reversed-phase (RP) compatible solvents and additives only. TIP The charged aerosol detector can also be used in a Vanquish system with normal-phase (NP) compatible solvents and additives if the system modules have been modified for NP applications.
Page 30 • Safety 2.4.2 Allowed pH ranges Allowed pH ranges (standard system configuration): System Allowed Remarks (standard pH ranges configuration) Vanquish Core 1-13 • pH values less than 2: Applicable only for short- term use of organic acids (for example, TFA). Flush the system thoroughly after these applications.
Page 31 • Safety 2.4.4 Further information • For details about the materials that are used in the analytical flow path of the device, see the Specifications chapter in this manual. For information about the materials that are used in the flow path of the other modules in the Vanquish system, refer to the Specifications chapter in the Operating Manual for the modules.
Page 32 • Safety Compliance information Thermo Fisher Scientific performs testing and evaluation of its products to ensure compliance with applicable regulations. When the device is delivered to you, it meets pertinent electromagnetic compatibility (EMC) and safety standards as described in this manual. Changes that you make to the device may void compliance with one or more of these EMC and safety standards.
Page 33 • Device overview 3 Device overview This chapter introduces you to the device and the main components. Charged Aerosol Detectors (VF-D21, VH-D21) Page 33 Operating Manual...
Page 34 • Device overview Detector features The detector comprises the following main features: • Charged aerosol detection (CAD) method for liquid chromatography for detection of all non-volatile and many semi-volatile analytes or volatile analytes that have a lower vapor pressure than the eluent •...
Page 35 • Device overview Detector configurations The charged aerosol detector is available in two variants: VF-D21 and VH-D21. The VF-D21 is designed to be used in a Vanquish system only. The VH-D21 can be used in the Vanquish system as well as in other configurations.
Page 36 • Device overview Figure 2: Integrated Vanquish LC system configuration Description Display (optional) Autosampler Pump System base System controller (optional) Charged aerosol detector Solvent rack Page 36 Charged Aerosol Detectors (VF-D21, VH-D21) Operating Manual...
Page 37 • Device overview External configuration with analog output The characteristics of the external configuration with analog output are: • Includes VH-D21 • Installation with system base, solvent rack, Vanquish User Interface (system controller and display), and analog output board • Communication with system controller via system interlink •...
Page 38 • Device overview External configuration with digital output The characteristics of the external configuration with digital output are: • Includes VH-D21 • Installation with system base and solvent rack • Data collection and device control with digital data communication (USB) •...
Page 39 • Device overview Configuration change The configurations can be changed as shown in the table below. Configuration Changeable to External configuration with analog • Integrated Vanquish LC system configuration output • External configuration with digital output External configuration with digital Integrated Vanquish LC system configuration output If you want to change your configuration, contact the Thermo Fisher...
Page 40 • Device overview Operating principle The detector is designed for charged aerosol detection with full compatibility with HPLC and UHPLC applications. In charged aerosol detection, the detection is based on measuring charge that is proportional to the amount of analyte, and independent of the chemical structure of the analyte.
Page 41 • Device overview Mobile phase from the column enters the detector (no. 1) and flows into the nebulizer (no. 2). Pressurized gas streams through the gas inlet (no. 3) and passes through the carbon gas filter and then the HEPA gas filter (no. 4). In the nebulizer (no. 2), the filtered gas and the mobile phase merge at the nebulizer tip to create an aerosol within the spray chamber (no. 5).
Page 42 • Device overview Interior detector components The user-accessible components of the detector are located directly behind the front doors: Figure 6: Interior view Description Keypad with status indicators Gas filter assembly behind the protective cover Leak tray Diverter valve Nebulizer Nebulizer label with serial number and calibrated gas pressure Waste port Type label, indicating the module name, serial number, part number, and revision number (if any)
Page 43 • Device overview 3.4.1 Diverter valve The detector is equipped with a 3-position diverter valve. The valve can be used to direct the flow to the following parts: • Nebulizer • External devices to bypass the detector • Waste port Figure 7: Diverter valve ports Description Waste...
Page 44 • Device overview 3.4.2 Nebulizer The FocusJet nebulizer is the liquid flow inlet of the charged aerosol detector. Eluate from the column flows into the nebulizer and merges with filtered gas that is supplied to the nebulizer. Due to the concentric flow design of the nebulizer, an aerosol is produced within the spray chamber.
Page 45 • Device overview 3.4.4 Waste port The waste port on the front panel collects waste from the diverter valve and from internal drain devices and has two openings. The upper opening is used to route liquid from the diverter valve to waste. The lower one is used to route waste liquid to a waste container.
Page 46 • Device overview Additional components for external configurations 3.5.1 Solvent rack The solvent rack provides a secure location for placing solvent reservoirs. It can store, for example, 6 x 1 L of solvent and 2 x 0.25 L of wash liquid. Only use the solvent rack for liquid reservoirs. Figure 11: Solvent rack (front view) The front side of the solvent rack is magnetic.
Page 47 • Device overview Figure 12: System base (front and rear view) Description Blind cap/Vanquish System Controller power button If a Vanquish system controller is installed, the power button allows power on/off control of the system controller. System power button Allows power on/off control for all modules of the system that are connected to the system base via the System Interlink port.
Page 48 • Device overview Waste management and leak detection As leaks are a potential safety issue, the charged aerosol detector provides the following: Waste management The diverter valve and the waste port on the front panel serve as waste management devices. Figure 13: Waste management devices Description Diverter valve...
Page 49 Operating Manual. Details on control and operation of the device are available in the Chromeleon Help. TIP The device can be operated also with other data systems, such as Thermo Scientific Xcalibur . In this case, installation of additional software is required in addition to the data system software. For details, contact the Thermo Fisher Scientific sales organization.
Page 50 • Device overview Page 50 Charged Aerosol Detectors (VF-D21, VH-D21) Operating Manual...
Page 51 • Unpacking 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of delivery. Charged Aerosol Detectors (VF-D21, VH-D21) Page 51 Operating Manual...
Page 52 • Unpacking Damaged packaging, defective on arrival Inspect the shipping container for signs of external damage and, after unpacking, inspect the device for any signs of mechanical damage that might have occurred during shipment. If you suspect that the device may have been damaged during shipment, immediately notify the incoming carrier and Thermo Fisher Scientific about the damage.
Page 53 • Unpacking Scope of delivery The following items are included in the delivery of the detector: • Detector with pre-installed nebulizer and capillaries • Ship kit • Power cord • Operating manual (downloadable from customer manual website) For information on contents of the ship kit or reordering parts, see Accessories, consumables and replacement parts (} page 239).
Page 54 • Unpacking Unpack the detector CAUTION—Heavy load, bulky device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: • Physical handling of the device, including lifting or moving, requires a team effort of two persons.
Page 55 • Unpacking 4. Place the device on a stable surface. 5. If applicable: Remove any additional packing material. Some surfaces including the doors of the device are covered by a protective film during shipment. Leave any protective films attached to the surfaces of the device until it is properly positioned in the system stack.
Page 56 • Unpacking Unpack the system base and solvent rack Only applicable to the external configuration with analog output and the external configuration with digital output. To unpack the solvent rack and system base, follow these steps: 1. Open the shipping container. 2.
Page 57 • Installation 5 Installation This chapter specifies the requirements for the installation site and describes how to install the detector. Charged Aerosol Detectors (VF-D21, VH-D21) Page 57 Operating Manual...
Page 58 • Installation Safety guidelines for installation Pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety precautions (} page 23). CAUTION—Heavy load, bulky device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: •...
Page 59 • Installation Install the device A Thermo Fisher Scientific service engineer installs and sets up the Vanquish system, including all modules and options or parts shipped with them. The service engineer checks that the installation is correct and that the Vanquish system and modules operate as specified. The engineer also demonstrates the basic operation and main features.
Page 60 • Installation 6. Set up the device in the software. See Set up the device in the software (} page 106). 7. Prepare the system for operation. Refer to the Vanquish System Operating Manual. 8. Recommended: Perform Instrument Installation Qualification. In the Chromeleon software, a wizard is available to guide you through the qualification process.
Page 61 • Installation Site requirements An appropriate operating environment is important to ensure optimal performance of the device. This section provides important requirements for the installation site. Note the following: • Operate the device only under appropriate laboratory conditions. • The device is intended to be part of the Vanquish system or part of a third-party HPLC system.
Page 62 • Installation Workbench requirements Part Requirements Site for the The workbench must stand in a secure and level position workbench that is free of vibrations. Workbench • Sturdy • Stable (non-tilting) • A height that ensures convenient access to the interior of each device in the system.
Page 63 • Installation 5.3.2 Power considerations The power supply of the device has wide-ranging capability, accepting any line voltage in the range specified for the device. To avoid power fluctuations, the use of an uninterruptible power supply (UPS) is recommended. CAUTION—Electric shock or damage to the device Connecting the device to a line voltage higher or lower than specified could result in personal injury or damage to the device.
Page 64 • Installation WARNING—Electric shock or damage to a product Misuse of the power cords could cause personal injury or damage the instrument. Use the power cords provided by Thermo Fisher Scientific only for the purpose for which they are intended. Do not use them for any other purpose, for example, for connecting other instruments.
Page 65 • Installation Vibration and mechanical shocks Vibrations and mechanical shocks may affect the performance of the system. Therefore, the installation site should be free of vibrations. Avoid mechanical shocks and avoid placing the system in locations where vibrations are caused by other instruments. Electromagnetic interference •...
Page 66 • Installation In addition to the operating conditions for ventilation stated in Operating conditions (} page 64), make sure that the location fulfills the following requirements. • Operate the detector near the ventilation sources. Do not extend the exhaust gas tubing shipped with the detector. •...
Page 67 • Installation NOTICE—Gas specifications The requirements and specifications for the gas supplied to the detector have major impact on the detector performance. Observe the following notes: • If the gas supply pressure is varying or dropping below the specified pressure range, it can impair the performance of the detector or even damage the detector.
Page 68 • Installation • The gas consumption of the detector is typically less than 5.0 SLPM. • The supply gas must be free from water vapor, free from particles, and must not contain non-volatile hydrocarbons, such as compressor oils. If necessary, use a sub-micron particle filter or carbon filter in combination with a water condensation trap located close to the gas source.
Page 69 • Installation Access the interior components To access the interior components in the device, open the front doors. To allow easy access from the front, the user-accessible components and flow connections in the device are located directly behind the doors. Figure 16: Access the interior components Charged Aerosol Detectors (VF-D21, VH-D21) Page 69...
Page 70 • Installation Setting up the hardware This section describes how to install the detector into the Vanquish system stack (integrated Vanquish LC system configuration). The external configuration with analog output and the external configuration with digital output must be set up by service personnel certified by Thermo Fisher Scientific.
Page 71 • Installation 3. Unlock the system base by turning the locking tools as shown below. In the unlocked position, the locking tools cannot be removed from Kommentar: ursprünglich blaue Pfeile hier übermalt the system base. Figure 18: Unlocking the system base 4.
Page 72 • Installation 5.5.2 Remove and install the carrying handles 5.5.2.1 Remove the carrying handles 1. Loosen the screws on the carrying handles, using a screwdriver (Torx T20). Do not remove the screws from the carrying handles completely. Figure 20: Carrying handles Component Carrying handles Attachment screw (one on each carrying handle)
Page 73 • Installation 5.5.3 Arrangement in the integrated Vanquish LC system configuration In this configuration, the detector is part of the Vanquish system. The system modules are arranged in a system stack. Figure 22: Vanquish system, configuration with charged aerosol detector and diode array detector (example) Description Solvent rack Charged aerosol detector...
Page 74 • Installation Requirements This configuration requires other Vanquish modules to be installed in the stack and interconnected via system interlink cables. Install the detector into a Vanquish stack Depending on the system configuration, follow the steps as required: System configuration Steps If the charged aerosol detector Follow the instructions in the Vanquish Operating...
Page 75 • Installation 5.5.4 Connecting the device Electric connectors The following electric connectors are provided on the device: VH-D21-A 255 VA 3.15 AT 2025-07 Figure 23: Electrical and gas connectors on the right side of the detector Description Rating plate, indicating the serial number, part number, module name, revision number (if any), and the line and fuse rating Main power switch (on/off control) Fuse holder...
Page 76 • Installation Description USB (Universal Serial Bus) hub ("A" type connector) Allows connection to other modules in the Vanquish system USB port ("B" type connector) Allows connection to other modules in the Vanquish system or the computer on which the chromatography data system is installed, such as Chromeleon For information on the connectors on the system base, see System base...
Page 77 • Installation Follow these steps NOTICE • Never use defective communication cables. If you suspect that a cable is defective, replace the cable. • To ensure trouble-free operation, use only the cables provided by Thermo Fisher Scientific for connecting the device. 1.
Page 78 • Installation Integrated Vanquish LC system configuration System Follow these steps configuration If the charged Follow the instructions in the Vanquish System Operating aerosol detector is Manual to set up the USB and system interlink connections in the only detector in the system.
Page 79 • Installation The USB connections are represented by dotted lines and the system interlink connections by solid lines. Figure 25: Interface cable connections in the Vanquish system with charged aerosol detector and diode array detector Description System base Pump Autosampler Diode array detector Charged aerosol detector Column compartment Connection to computer...
Page 80 • Installation External configuration with analog output and external configuration with digital output 1. Optional: Connect 2 digital I/O cables from the third-party installation kit to the third-party HPLC system. Refer to the Charged Aerosol Detector Third-Party Kit Installation Guide. 2.
Page 81 • Installation Figure 26: Interface cable connections in external configuration with analog output and external configuration with digital output Description Digital I/O connections to the third-party HPLC system (optional) External configuration with digital output only: Connection to the computer External configuration with analog output only: Connection to an analog device Charged aerosol detector System controller...
Page 82 • Installation 5.5.4.2 Connect power cords This section provides details on how to connect the modules to the power source. Do not turn on the system or modules yet. NOTICE Condensation in a device can damage the electronics. • Before connecting the devices to the power line, be sure that no condensation is present in the devices.
Page 83 • Installation Tools required Tubing cutter (optional) Preparations 1. Observe the site requirements for the gas supply and the ventilation (see Ventilation requirements (} page 65) Gas supply requirements (} page 66)). 2. Check the gas tubes and elbow connectors. Observe the following: Part Requirements Gas tubes •...
Page 84 • Installation Figure 27: Connecting gas tubes (left: to the gas inlet, right: to the gas exhaust) Description Gas inlet elbow connector Optional: Connection stem (to gas inlet port) Optional: Connector (for gas inlet tubing) Optional: Short piece of gas inlet tubing (must be cut from the supplied gas inlet tubing) Optional: Gas supply on/off valve Optional: 1/4"...
Page 85 • Installation 2. On the left port (gas inlet), push the gas inlet tubing or the connection stem of the gas inlet elbow connector into the port. Figure 28: Connecting the tubes (here: with elbow connectors but without gas supply on/off valve or adapter) Description Gas inlet port Gas inlet elbow connector (optional) with gas inlet tubing...
Page 86 • Installation Set up the liquid flow connections This section describes how to set up the flow connections to and from the device and additional flow connections, if required. 5.6.1 General information and guidelines When setting up flow connections, follow these rules and recommendations: Flow connections can be filled with hazardous substances.
Page 87 • Installation TIP Components or connections in the flow path to other system modules may be closed with plugs to protect the component or connection during transport. When you remove the plugs to connect the device in the system, keep the plugs.
Page 88 • Installation Tubing brackets Tubing brackets are available for holding the tubing in place. Slip the bracket side onto the drain pipe. Figure 30: Tubing bracket (left), tubing bracket installed (right) System arrangements with more than one pump The number of tubes may exceed the capacity that the tubing guides can hold.
Page 89 • Installation 5.6.3.2 Connect Viper capillaries This section describes how to connect Viper™ capillaries. All Viper flow connections in the Vanquish system are designed to be finger-tight. NOTICE • Tighten or loosen Viper capillaries only with your fingers. Do not use tools other than the knurl that comes with the capillary.
Page 90 • Installation Resolve leakage of Viper fittings with knurls 1. Tighten the connection a little more. 2. If leakage continues, remove the capillary. 3. Clean the capillary ends carefully by using a lint-free tissue wetted with isopropanol. 4. Reinstall the capillary. 5.
Page 91 • Installation Parts affected Figure 32: Detector waste line parts (left: detector seen from the left side; right: detector and system base seen from the right side) Description Waste port Waste port outlet Waste connection piece Short waste line Long waste line Waste L-piece Inlet for the waste line of another detector Detector waste outlet in the system base...
Page 92 • Installation 5. Locate the outlet for the detector waste line in the system base (see figure below). 6. Route the free end of the long detector waste line through the Anmerkung: blaue Pfeile hier mit roten Pfeilen übermalt detector waste outlet. Figure 33: Routing the long detector waste line through the detector waste outlet in the system base 7.
Page 93 • Installation 8. Make sure that the long detector waste line is positioned straight in the tubing guides and does not form a siphon. 9. Make sure that the short detector waste line forms a slight downward slope. 10. Ensure proper drainage of waste (see next section). 11.
Page 94 • Installation 5.6.5 Connect capillaries (one-detector setup) If you set up the flow connections for the first time, most of the capillaries are pre-installed. You only need to connect the inlet capillary between the column and the diverter valve inlet port. If you set up the flow connections after having removed the capillaries, follow all the instructions in this section.
Page 95 • Installation Parts required The table shows which parts are required: Connection Parts required Column – diverter valve Viper capillary, I.D. x length 0.1 x 350 mm, MP35N (from the ship kit) Diverter valve – waste port Pre-installed – only needed if the capillaries were removed.
Page 96 • Installation 5. If the capillaries are not connected to the diverter valve: a) On the diverter valve, remove the plug from the Inlet, Nebulizer, Waste, and optionally from the Bypass port. Keep the plugs for later use. b) Connect the correct capillary between the Waste port of the diverter valve and the waste port inlet (see picture below).
Page 97 • Installation 5.6.6 Connecting capillaries (two-detector setup) The charged aerosol detector can be used in series or parallel liquid flow configuration with other detectors (for example, an optical detector or mass spectrometer). Flow connection capillary ports Figure 37: Flow connection capillary ports Description Diverter valve Waste port Diverter valve Nebulizer port...
Page 98 • Installation 5.6.6.1 Guidelines for multi-detector setups Observe the following guidelines for multi-detector setups: NOTICE—Damage to sensitive flow cells of the Vanquish Horizon diode-array detector, Vanquish refractive index detector and the Vanquish fluorescence detector Increased backpressure and pressure spikes created by the charged aerosol detector and its diverter valve installed downstream of the flow cell can destroy the pressure-sensitive flow cells.
Page 99 • Installation 5.6.6.2 Connect the charged aerosol detector to another detector These instructions describe how to connect the charged aerosol detector to an upstream diode array detector VC-D11, for example. If you set up the flow connections for the first time, most of the capillaries are pre-installed.
Page 100 • Installation 4. Secure the capillary: a) On the leak tray of the charged aerosol detector, pull the capillary clip carefully to the right to open the clip. b) Position the capillary behind the capillary clip. c) Carefully release the clip and take care not to clamp the capillary.
Page 101 • Installation Figure 39: Connecting capillaries between the flow cell of the VC-D11, VC-D12, VF-D11 detector and the diverter valve 2. Optional: Connect a capillary between the Bypass port of the diverter valve and an external device, such as a mass spectrometer. 5.6.7 Guiding liquid leaks to waste Leaking liquids of the device are collected in the leak tray, where they...
Page 102 • Installation Follow these steps 1. Push an elbow connection piece into the system drain port as far as it goes in to establish a self-sealing connection. Figure 40: Connected system drain tubing and elbow connection piece Description Funnel for system drainage Detector waste outlet Detector waste line Elbow connection piece...
Page 103 • Installation Ensure proper drainage of waste 1. Position the waste container below the level of the system stack. To prevent solvent vapors from evaporating back into the system, make sure that the distance is sufficient. 2. Make sure the drain tubing is neither bent nor pinched. 3.
Page 104 • Installation Turn on the device Before turning on the power to a Vanquish system module for the first time, verify that the chromatography software is installed on the data system computer. When the power is turned on, the required USB drivers are automatically found and the Windows™...
Page 105 • Installation 6. Turn on the gas. 7. Switch the diverter valve to the Nebulizer position. Turn off the device with the main power switch, when instructed to do so, for example, during maintenance. Pressing the system power button will not be sufficient to turn off the power to the device completely. See also Power on/off control (} page 117) Charged Aerosol Detectors (VF-D21, VH-D21)
Page 106 Module on the Edit menu. 4. In the Add module to instrument dialog box, on the Manufacturers list, select Thermo Scientific HPLC: Vanquish and on the Modules list, select Vanquish Charged Aerosol Detector P. 5. On the General configuration page, make sure that simulation mode is deactivated and click Browse to select the module address.
Page 107 • Installation e) Optional: Change the pump flow emergency shutoff (Emergency Mode) setting. It defines the behavior if the gas flow is turned off or if the gas pressure is too low. Option Description Switch Off the Pump Default Flow and Switch The pump flow will be switched off and the diverter valve Diverter Valve to Waste will switch to Waste.
Page 108 • Installation Page 108 Charged Aerosol Detectors (VF-D21, VH-D21) Operating Manual...
Page 109 • Operation 6 Operation This chapter describes the elements for device control, provides information for routine operation and for shutdown. Charged Aerosol Detectors (VF-D21, VH-D21) Page 109 Operating Manual...
Page 110 • Operation Introduction to this chapter The information in this chapter assumes that the initial setup of the device has already been completed. If this is not the case, see the instructions in Installation (} page 57). Control with Chromeleon For a basic description of instrument control and automated sample analysis with the Chromeleon software, refer to the Vanquish System Operating Manual.
Page 111 • Operation Safety guidelines for operation When operating the device, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety precautions (} page 23). WARNING—Fire hazard or damage to the detector When using flammable solvents, the use of air as supplied gas may pose a fire hazard.
Page 112 • Operation NOTICE Pay attention also to the following guidelines: • Observe the requirements for gas supply and ventilation. See Ventilation requirements (} page 65) Gas supply requirements (} page 66). • Contamination of the mobile phase can damage the detector. Avoid contamination of the mobile phase. See Mobile phase guidelines for the detector (} page 118).
Page 113 • Operation Control elements The device is designed to be operated from a computer running the chromatography data system or from the Vanquish User Interface. In addition, the following elements are available on the device: • Keypad: The keypad buttons allow you to perform certain functions directly from the device.
Page 114 • Operation MUTE ALARM Beeping alerts you when the device detects a problem, for example, leakage. To turn off the beep for the current alarm, press this button. Eliminate the source for the alarm within 10 minutes. Otherwise, beeping starts again. If the device detects a different problem, beeping alerts you again immediately.
Page 115 • Operation When the buttons are deactivated The button functionality is as shown in the table. Situation Enabled/disabled buttons Data acquisition is running • Enabled function: MUTE ALARM button Allows to turn off the beep for the current alarm. Script is running (external configuration with analog •...
Page 116 • Operation LED bar Description Yellow The LED bar is yellow if one of the following applies: • Self-test is running after power-up. • The detector is connected and equilibrated but the gas flow is turned off. • External configuration with analog output: The diverter valve is not in Nebulizer position.
Page 117 • Operation Power on/off control This section describes the general power on/off mechanism of the main power switch of the system modules and the system power button on the system base. The correct and complete shutdown procedure is described in the relevant sections. For easier handling, you can use the power button on the front left of the Vanquish system base (system power button) for power on/off.
Page 118 • Operation Mobile phase guidelines for the detector Observe the guidelines in the following sections for mobile phase use with the charged aerosol detector: WARNING—Fire hazard or damage to the detector When using flammable solvents, the use of air as supplied gas may pose a fire hazard.
Page 119 • Operation 6.5.2 Mobile phase selection Minimizing non-volatile matter in the mobile phase NOTICE Long-term exposure of the detector to non-volatile mobile phases will damage the detector. Do not use non-volatile buffers. Observe the following guidelines to minimize the concentration of non- volatile matter in the mobile phase: •...
Page 120 • Operation • Water or highly aqueous (< 5% organic modifier) mobile phases are highly susceptible to microbial growth. Prepare such mobile phases freshly on a daily basis. • Select an optimal evaporation temperature to selectively evaporate background components of the mobile phase. Observe the guidelines in Evaporation tube temperature (} page 150).
Page 121 • Operation 6.5.4 Additives General guidelines for additives • Use only high-purity volatile additives for the mobile phase, such as pH modifiers, buffer salts and ion pairing reagents. • Avoid using non-volatile additives, such as citrate, phosphate, potassium, sodium, sulfonic acids and Tris. •...
Page 122 • Operation • If using cationic ion pairing reagents, such as triethylamine (TEA), practice the same precautions as with anionic pairing reagents to avoid formation of non-volatile salts with other additives and to stay within the range of column and detector pH compatibility. •...
Page 123 • Operation Preparing the detector for operation This section gives information on any additional steps that are required to prepare the device for operation and sample analysis. NOTICE Flush the system flow path thoroughly before operating the device for the first time: •...
Page 124 • Operation Check the setting of the nebulizer pressure and the nebulizer serial number Set the nebulizer gas pressure and the nebulizer serial number (} page 124) ↓ External configuration with analog output only: Define the pump flow emergency shutoff behavior Defining the pump flow emergency shutoff behavior (} page 126) ↓...
Page 125 • Operation TIP The nebulizer serial number can be found on the following locations: • Nebulizer certificate that is shipped with the detector or with the replacement nebulizer • Label attached to the front panel or delivered together with the replacement nebulizer •...
Page 126 • Operation Follow these steps for the external configuration with analog output Follow this procedure before initial operation. If you replace the nebulizer and you use the script (C50 - CAD - Replace Nebulizer), you set the gas pressure and nebulizer serial number with the script. 1.
Page 127 • Operation External configuration with analog output By default, the diverter valve will be switched to Waste if the gas flow is too low or turned off. If the pump is connected to the detector via digital I/O, the pump flow will also be turned off. The pump flow emergency shutoff behavior can be changed so that either the pump flow is turned off or the diverter valve is switched to Waste.
Page 128 • Operation Turn on the detector gas flow and the pump flow 1. Make sure that you observe the site requirements for the gas supply and ventilation. See Gas supply requirements (} page 66) Ventilation requirements (} page 65). 2. Make sure that the following prerequisites are met: The nebulizer is installed.
Page 129 • Operation 6.6.1.4 Flush the system and detector When Perform this procedure in the following cases: • After initial installation • After a long-term shutdown Procedure overview Flush the system without the charged aerosol detector connected to the flow path. Flush the system without the charged aerosol detector (} page 129).
Page 130 • Operation Flush the system including the charged aerosol detector and connected nebulizer 1. Connect the nebulizer to the flow path. 2. Set the diverter valve to NEBULIZER position via the chromatography data system, Vanquish User Interface or the keypad button. 3.
Page 131 • Operation System equilibration System equilibration should include the following operations: • Purging the pump (all channels, including those not used for the application) • Flushing the entire chromatography system with the starting solvent to rinse out any solvent from a previous analysis run •...
Page 132 • Operation Important operating parameters The commands and parameters described in the table should be considered for simple routine operation of the device. You can usually access these parameters from the chromatography data system user interface and the Vanquish User Interface. 6.7.1 Important Chromeleon operating parameters If a parameter listed below is not available in the Chromeleon software,...
Page 133 • Operation Pump flow emergency shutoff parameters Parameters Action/Description Emergency Mode Defines the behavior of the detector if it detects a low or no gas flow. Can be set to the following values: • Turn off pump flow and switch the diverter valve to Waste (default) •...
Page 134 • Operation Evaporation tube temperature parameters Parameters Action/Description Evaporation Temperature Turn temperature control for the evaporation tube on or Control It is enabled by default when the detector is shipped. Evaporation Temperature Specifies the setpoint for the evaporation tube Nominal temperature.
Page 135 • Operation Diverter valve parameters Parameters Description Diverter valve position Current Position displays and sets the position of the diverter valve. Values are: 1: Waste 2: Nebulizer 3: Bypass By default, the Bypass position is not available. Before using it, activate it in the Chromeleon Instrument Configuration Manager (see Set up the device in the software...
Page 136 • Operation 6.7.2 The detector in the Vanquish User Interface Integrated Vanquish LC system configuration and external configuration with digital output In these configurations, parameters on the Vanquish User Interface are read-only. The detector is configured and controlled via a chromatography data system.
Page 137 • Operation 6.7.2.3 System module box The detector module box on the home panel shows the following parameters: • Current of signal channel 1 • Actual evaporation tube temperature • Detector status or position of the diverter valve when the detector is idle •...
Page 138 • Operation Parameters Description Coupling Mode Offset Only available if Temperature Coupling Mode is activated. The offset can be adjusted from -5 °C to +5 °C. Default: +5°C Example: If the evaporation tube temperature is set to 50 °C and the offset to +5 °C, the charging detection module temperature is controlled to 55 °C.
Page 139 • Operation Parameters Description Temperature Coupling Turns the temperature coupling mode on or off Mode The temperature coupling mode adjusts the temperature of the charging detection module to that of the evaporation tube by an adjustable offset. This mode might help to prevent recondensation or evaporation of the dried aerosol if the gas flow is saturated with volatile eluents and additives.
Page 140 • Operation Installation/configuration scripts These scripts are only available for the external configuration with analog output. Script Description A50 - CAD - Check Guides you through various steps to check if the digital I/O Digital I/O wires are correctly connected and working A51 - CAD - Configures the analog output board Configure Analog...
Page 141 • Operation Script Description Service / Wellness Options: Settings • If you activate the Show Current Values option, the current counter values after the last maintenance are displayed. • Default If activating the Show Values and Limits option, the following is displayed: Current counter values after the last ¨...
Page 142 • Operation Script Description Autozero Only active if Signal Data Acquisition is selected Turns the autozero function on or off • On: Performs an automatic null balancing for channel 1 before data acquisition. The current detector signal is interpreted as 0 at the current range in use.
Page 143 • Operation Script Description Signal Output Defines the channel to record Mapping Options: • Background Data Acquisition: Records and monitors the background current of channel 1 during data acquisition (no filter constant or autozero applied) • Signal Data Acquisition: Records signal channel 1 with or without applied autozero and filter constant depending on the Autozero setting.
Page 144 • Operation Maintenance scripts These scripts are available for all configurations. For the external configuration with analog output, the maintenance scripts display the replacement due dates. For the integrated Vanquish LC system configuration and the external configuration with digital output, maintenance scripts are available as animations.
Page 145 • Operation Service script Script Description E03 - Download Collects a Chromeleon backup file (cmbx) and service log file for Service Data Technical Support. Charged Aerosol Detectors (VF-D21, VH-D21) Page 145 Operating Manual...
Page 146 • Operation Optimize the performance of the device This section provides information for best performance of the device and gives hints on what you can do to optimize the performance further. 6.8.1 General guidelines To optimize the performance of the device, consider the following general guidelines: •...
Page 147 • Operation • When the mobile phase was stored over a period of time, high background current may be observed. Replace old mobile phase with freshly prepared mobile phase. • If you use the detector for the analysis of complex biological samples, proper sample preparation is especially important.
Page 148 • Operation • If the data collection rate is too high, data files may need more disk space and post-run analyses may require more processing time. • If all peaks are relatively wide, select a lower data collection rate. This saves disk space and allows for a faster display of data in the Chromeleon software.
Page 149 • Operation Evaporation tube temperature • The signal channel records and monitors the actual temperature of the evaporation tube during data acquisition. • It is recommended to always record the evaporation temperature signal channel for troubleshooting purposes. • If a problem occurs, the information from the signal channel can provide helpful information to identify and eliminate the source for the problem.
Page 150 • Operation Guidelines for selecting a power value To optimize the power value, observe the following guidelines: • An optimal power value improves the linearity of response over a given range of interest. • Selecting the optimal value depends primarily on the analysis conditions and the desired range of quantitation.
Page 151 • Operation • When the temperature was changed, the evaporation tube requires time to equilibrate. Measurements will only start if the evaporation tube has fully equilibrated. Integrated Vanquish LC system configuration and external configuration with digital output only: If the Evaporation Wait For Temp Ready parameter is disabled, ¨...
Page 152 • Operation • Perform screening runs at two evaporation tube temperature settings. VH-D21 detector: Use a minimum of two evaporation tube ¨ temperatures. Typically, 35 °C and 40 °C are appropriate if the temperature range is unknown. If required, perform additional tests in a maximum of 5 °C increments. VF-D21 detector: Use two evaporation temperatures, depending ¨...
Page 153 • Operation 6.8.8 Baseline noise Baseline noise can originate from many factors. To minimize the baseline noise, observe the following: • The quality of the mobile phase has a major impact on the performance of the detector. Only use mobile phases of the highest quality available.
Page 154 • Operation Shut down the device If the device will not be operated for some time, follow the instructions in this section to shut down the device. 6.9.1 Short-term shutdown (interruption of operation) To interrupt operation of the device for a short period (short-term shutdown), for example, overnight, observe these guidelines for the Vanquish system modules, as required by your system arrangement: •...
Page 155 • Operation 6.9.2 Long-term shutdown To interrupt operation for a longer period, follow the instructions below. NOTICE Observe the following to avoid damage to the charged aerosol detector: • Always flush the detector with an appropriate high-quality solvent before interrupting operation. Follow the steps below. •...
Page 156 • Operation If no additive is used If an additive is used If using normal-phase (NP) compatible solvents and additives: Recommendation: Flush the system with 1. Flush the system with 3-10 system isopropanol. volumes of an appropriate pure solvent, for example, for 10 minutes at 1 mL/min Do not use 100% acetonitrile or 100% (10 mL).
Page 157 • Operation 7. The step depends as follows: Situation Steps Device and all other system components 1. Turn off the system with the system remain in the system stack and are to be power button on the system base. turned off 2.
Page 158 • Operation 3. Turn on the device. Observe the following: Situation Action If the device remained in the system 1. If a system controller is installed: Turn stack and all system modules were on the system controller on the system turned off.
Page 159 • Maintenance and service 7 Maintenance and service This chapter describes the routine maintenance and the service procedures that the user may perform on the detector, system base and solvent rack. For information on the procedures for the system controller and the display, refer to the Vanquish User Interface Installation Guide.
Page 160 • Maintenance and service Introduction to maintenance and service This chapter describes the routine maintenance and service and repair procedures that the user may perform. Additional maintenance or service procedures must be performed only by service personnel certified by Thermo Fisher Scientific (for brevity, referred to as Thermo Fisher Scientific service personnel).
Page 161 • Maintenance and service Safety guidelines for maintenance and service When performing maintenance or service procedures, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety precautions (} page 23). WARNING—High voltage High voltages are present inside the device that could cause an electric shock.
Page 162 • Maintenance and service CAUTION—Hydrostatic pressure Solvent may spill when you open the flow path. This is due to hydrostatic pressure in the system when the solvent reservoirs are located above the pump outlet. Before you loosen a connection in the flow path: •...
Page 163 • Maintenance and service General rules for maintenance and service For successful maintenance and service procedures, follow the rules and recommendations below. General rules • Before starting maintenance or service procedures, shut down the device when instructed to do so. NOTICE—Detector damage If the detector is in error state, turning off the detector without performing a proper shutdown procedure may lead to detector flooding...
Page 164 • Maintenance and service Opening flow path connections • Before opening the flow path to replace capillaries in the system, turn off the pump flow and wait until the system pressure is down to zero. • Dirty components can contaminate the chromatography system. Contamination leads to poor performance of the modules and entire system or can even cause damage to the modules and system.
Page 165 • Maintenance and service Routine and preventive maintenance Optimum device performance, maximum uptime of the device, and accurate results can be obtained only if the device is in good condition and properly maintained. 7.4.1 Maintenance plan Perform the maintenance procedures in the table on a regular basis. The frequency given in the table is a suggestion.
Page 166 • Maintenance and service 7.4.2 Flushing the detector between analyses Observe the following guidelines to prevent build-up or accumulation of residue from the mobile phase, sample matrix and analytes within the detector between the analyses. This helps maintain consistent response, optimum performance and reduced detector downtime: •...
Page 167 • Maintenance and service Parts required • Suitable cleaning detergent (or disinfectant) • Purified water • Lint-free cloths or wipes CAUTION—Explosive gas mixtures from alcoholic cleaning detergents Alcohol-containing cleaning detergents may form flammable and explosive gas mixtures with air. • Use such cleaning detergents only when required and only in adequately ventilated rooms.
Page 168 • Maintenance and service Preparations 1. Turn off the pump flow and then the gas flow: a) Turn off the pump flow to the detector. b) Wait at least 30 minutes while the gas is turned on and the diverter valve is switched to Waste position. During the waiting period, the detector drains any remaining mobile phase inside the detector to waste.
Page 169 • Maintenance and service 7.4.4 Predictive Performance Chromeleon The Chromeleon software supports functions for estimating the lifetime of consumables and for monitoring and recording service and qualification information about the device. These functions are called Predictive Performance. They allow you to schedule maintenance procedures based on the actual operating and usage conditions of the device.
Page 170 • Maintenance and service Vanquish User Interface This section applies to the external configuration with analog output only. The VUI offers the functionality for estimating the lifetime of consumables and for monitoring and recording service and qualification information about the device. Maintenance and service intervals as well as warning limits are pre-defined that match the values given in Maintenance plan (} page 165).
Page 171 • Maintenance and service Replace the gas inlet and exhaust tubes CAUTION—Escaping gas Gas can escape from open gas connections when the detector gas flow and/or the gas supply is turned on. The escaping gas can pose a health and safety risk. •...
Page 172 • Maintenance and service 3. Switch the diverter valve to Waste or Bypass. 4. Turn off the detector with the main power switch. Follow these steps 1. On the gas port on the detector, disconnect the elbow connectors or the gas tubes from the port using the multitool. 2.
Page 173 • Maintenance and service Replace capillaries Parts required Replacement capillary Preparations 1. Flush the detector with the detector gas flow being turned on. For recommendations on flushing the detector, see Flushing the detector between analyses (} page 166). 2. Turn off the pump flow and then the gas flow: a) Turn off the pump flow to the detector.
Page 174 • Maintenance and service Replace the detector waste line Parts and tools required • Detector waste line • Waste connection piece • Waste L-piece • Tubing cutter Parts affected Figure 45: Detector waste line parts (left: detector seen from the left side;...
Page 175 • Maintenance and service Preparations 1. Flush the detector with the detector gas flow being turned on. For recommendations on flushing the detector, see Flushing the detector between analyses (} page 166). 2. Turn off the pump flow and then the gas flow: a) Turn off the pump flow to the detector.
Page 176 • Maintenance and service Replacing the nebulizer If using the integrated Vanquish LC system configuration or external configuration with digital output, follow the instructions in Remove the nebulizer (} page 176) Install the nebulizer (} page 179). If using the external configuration with analog output, run the following script: C50 - CAD - Replace Nebulizer.
Page 177 • Maintenance and service NOTICE The nebulizer is highly sensitive to contamination. Even minute particles on the nebulizer can lead to contamination and poor detector performance. Always wear powder-free gloves when you install or remove the nebulizer. NOTICE—Leaking nebulizer due to damaged O-rings Any movement in the nebulizer opening can damage the nebulizer O- rings.
Page 178 • Maintenance and service Follow these steps 1. Turn the nebulizer by 90 degrees counter-clockwise in the opening until it releases from the two locking points. Mind the correct orientation of the nebulizer when it is unlocked: Figure 46: Unlocking the nebulizer Description Gripping sides of the nebulizer on the top and bottom handle side Protruding attachment locks of the nebulizer on the left and right handle...
Page 179 • Maintenance and service 3. Install a new nebulizer. See Install the nebulizer (} page 179). Always keep the nebulizer opening closed with a nebulizer to prevent dust from entering the opening. 4. If the nebulizer is to be transported: Carefully pack the nebulizer so that the sensitive tip is protected.
Page 180 • Maintenance and service NOTICE To avoid damage to the nebulizer, do not disassemble the nebulizer. Parts and additional items required • Powder-free gloves • FocusJet nebulizer with dedicated nebulizer gas pressure certificate Preparations 1. Put on powder-free gloves. 2. Unpack the nebulizer carefully from the packaging. Locate the gas pressure certificate for the nebulizer in the packaging, it is needed later in the procedure.
Page 181 • Maintenance and service 4. If the liquid flow inlet of the nebulizer is protected by a protective tubing: Remove the protective tubing from the nebulizer spray pipe carefully. Do not touch the nebulizer tip with the protective tubing, as it can damage the tip. Keep the protective tubing to have it available when transporting the nebulizer.
Page 182 • Maintenance and service 2. Push the nebulizer into the opening until the spray pipe and sealing bushing are completely in the opening. The nebulizer handle must lie flush against the nebulizer opening. Figure 52: Completely inserting the nebulizer Description Opening for the nebulizer on the interior panel of the detector Spray pipe Sealing ring in the sealing bushing Page 182...
Page 183 • Maintenance and service 3. Turn the nebulizer by 90 degrees clockwise in the opening to lock the nebulizer. Check that the protruding sides on the nebulizer arrest behind the two locks of the opening and lie against the alignment pin. Figure 53: Locking the nebulizer in the detector Description Locks on the nebulizer opening...
Page 184 • Maintenance and service Replace the gas filter assembly If using the integrated Vanquish LC system configuration or the external configuration with digital output, follow the instructions in this section. If using the external configuration with analog output, run the script: C51 - CAD - Replace Gas Filter.
Page 185 • Maintenance and service NOTICE—Damage to the detector Turning off the gas flow too early may result in condensation and, thus, to deterioration of detector parts and/or flooding. Wait for at least 30 minutes before you turn off the gas flow to the detector.
Page 186 • Maintenance and service 2. Slightly loosen the two bottom screws on the protective cover. Leave the two screws attached loosely in the bores. Do not remove the screws. 3. Push the protective cover upward to release it from the two bottom screws (left image).
Page 187 • Maintenance and service 5. Pull the gas filter assembly out of the clamps. Figure 57: Pulling the gas filter assembly out of the clamps 6. With the new gas filter assembly, mind the correct orientation for the gas flow for installation. Observe the flow direction signs on the filters: The top gas filter must be the HEPA gas filter (white inner filter ¨...
Page 188 • Maintenance and service 8. Push the (push-in) elbow fittings into the gas connectors on the detector. Check that the connections are secure: Slightly pull on the fittings. If a fitting slides out, repeat this step. 9. Install the protective cover: Hold the protective cover onto the interior panel.
Page 189 • Maintenance and service 7.10 Diverter valve You may inspect the diverter valve for leakage or replace the stator and/ or rotor according to the maintenance plan. 7.10.1 Guidelines for handling the diverter valve To ensure optimum operation of the valve, observe the following guidelines: NOTICE •...
Page 190 • Maintenance and service Follow these steps NOTICE • Tighten or loosen Viper capillaries only with your fingers. Do not use tools other than the knurl that comes with the capillary. • To avoid damage to the capillary or connection, tighten and loosen the Viper capillaries only when the system pressure is down to zero.
Page 191 • Maintenance and service Parts required • Fitting screw • Stator and/or rotor • Powder-free gloves Preparations 1. Flush the detector with the detector gas flow being turned on. For recommendations on flushing the detector, see Flushing the detector between analyses (} page 166).
Page 192 • Maintenance and service 5. Loosen the 3 screws of the stator gradually with the screwdriver (Torx T15): a) Unscrew one screw by approximately 180°. b) Move to the next screw. c) Repeat previous steps until all 3 screws are completely loose and remove the screws.
Page 193 • Maintenance and service 7. Place the stator on a lint-free cloth. 8. If the o-ring of the rotor sticks to the stator, carefully remove it. 9. Grasp the rotor between index finger and thumb or between two index fingers and remove it from the valve. Figure 63: Removing the rotor Description Rotor...
Page 194 • Maintenance and service 12. Align the rotor so that the hole in the rotor is placed onto the long pin in the valve and place the replacement rotor with the attached o-ring into the valve. Figure 64: Installing a new rotor with aligned pin Description Rotor Rotor o-ring...
Page 195 • Maintenance and service 15. Tighten the 3 screws of the stator gradually with the screwdriver (Torx T15): 180° Figure 66: Tightening the stator screws a) Fasten one screw by approximately 180°. b) Move on to the next screw. c) Repeat the previous steps. d) Check the correct position of the stator lying flat in the valve.
Page 196 • Maintenance and service 7.11 Replace the main power fuses When Blown fuses Parts required Fuses (2 fuses, 3.15 AT, 250 V AC, slow-blow, 5 x 20 mm) from Fuses Kit Tools required Slotted screwdriver, any size between 3.3 mm and 5.5 mm is appropriate Preparations WARNING—Electric shock High voltages are present inside the device that could cause an electric...
Page 197 • Maintenance and service 1. Use the screwdriver to remove the fuse holder. 2. Replace the two fuses with new fuses of the specified type and current rating. Always replace both fuses. 3. Reinstall the fuse holder. 4. Reconnect the power cord to the power source and to the device. 5.
Page 198 • Maintenance and service 7.12 Update the device firmware The description in this section refers to the Chromeleon 7 Chromatography Data System. If you use the external configuration with analog output, a Thermo Fisher Scientific service engineer has to update the detector firmware. When Updating the device firmware might be required, for example, when a new firmware version is released that adds functionality or solves...
Page 199 • Maintenance and service NOTICE A firmware downgrade or incomplete firmware update may result in loss of functionality or malfunctioning of the device. • Do not interrupt communication between the Chromeleon software and the device at any time during the procedure. •...
Page 200 • Maintenance and service 7.13 Replace the doors When Damage of door TIP The maintenance procedures do not require that you remove the doors. If this should ever be required for a specific reason or procedure, follow the steps in this section. Parts required Replacement door Preparations...
Page 201 • Maintenance and service Figure 68: Door hinge aligned with groove Description Hinge on the housing Reception groove on the door 3. Slightly tilt the door to the outside, away from the housing, and remove the door. 4. To install the door, align the door with the hinges on the housing. Do not clamp tubing or capillaries between the door and the enclosure.
Page 202 • Maintenance and service 7.14 Replace the solvent rack This section only applies to the external configuration with analog output and the external configuration with digital output. If you want to replace the solvent rack in a Vanquish system stack, refer to the Vanquish Operating Manual.
Page 203 • Maintenance and service 9. Turn off the system with the system power button (lower button on the system base). 10. Turn off the system controller with its power button (upper button on the system base) if available. 11. Remove the liquid reservoirs from the solvent rack. Follow these steps 1.
Page 204 • Maintenance and service 7.15 Transport or ship the device If you want to transport the device to a new location (relocation) or if you need to ship the device, first prepare the device for transport, and then move or ship the device as required. Follow the instructions in this section.
Page 205 • Maintenance and service 5. Disconnect the gas inlet and exhaust tubes from the detector. See Replace the gas inlet and exhaust tubes (} page 171). 6. Disconnect the waste line from the detector. See Replace the detector waste line (} page 174). 7. Remove the device or slide-in module from the system stack as required: Situation Steps...
Page 206 • Maintenance and service 7.15.3 Dismount the stack of an external configuration Only applicable for the external configuration with analog output and the external configuration with digital output. When • Before replacing the system base • Before transporting or shipping the system Tools required Screwdriver, Torx T20, L-shaped Preparations...
Page 207 • Maintenance and service 4. Install the carrying handles. See Install the carrying handles (} page 72). 5. Lift the front part of the module or housing by using the carrying handles. Remove the module or housing from the stack towards the front and carefully place it on a stable surface.
Page 208 • Maintenance and service CAUTION—Possible contamination Hazardous substances may have contaminated the device during operation and may cause personal injury to service personnel. • Decontaminate all parts of the device that you want to return for repair. • Fill in and sign the Health and Safety Form. Thermo Fisher Scientific refuses to accept devices for repair if the Health and Safety Form is missing, incompletely filled in, or unsigned.
Page 209 • Maintenance and service 7.16 Replacing the slide-in module You can remove the slide-in module from the enclosure of a module for transporting or shipping purposes. The enclosure remains in the system stack. To return a defective module to the factory, install the slide-in module in the enclosure of the replacement module.
Page 210 • Maintenance and service Follow these steps 1. Loosen the four captive screws on the front left and front right of the device. Figure 71: Captive screws on the slide-in module (doors not shown) 2. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase.
Page 211 • Maintenance and service 4. Remove the slide-in module from the enclosure. The following steps require a team effort: a) Take the slide-in module on both sides from below. b) Pull the slide-in module from the rails towards the front. c) Place the slide-in module on a clean and stable surface.
Page 212 • Maintenance and service 7.16.3 Install the slide-in module CAUTION—Heavy load, bulky device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: •...
Page 213 • Maintenance and service Figure 73: Inserting the slide-in module 4. To facilitate tightening the slide-in module in the enclosure, pull out the module approximately 5 mm. 5. With the screwdriver, move each captive screw back and forth while pressing inward until the screw slips into the thread. 6.
Page 214 • Maintenance and service 7.16.4 Set up the slide-in module After you have installed the slide-in module in the enclosure, set up and restart the device. Follow these steps 1. Set up the slide-in module: a) Connect the slide-in module and set up flow connections. See Installation (} page 57).
Page 215 • Troubleshooting 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the device. Charged Aerosol Detectors (VF-D21, VH-D21) Page 215 Operating Manual...
Page 216 • Troubleshooting General information about troubleshooting The following features help you to identify that a problem occurred during operation of the system. Indicator Description Status indicators If the firmware of a system module detects a problem, the system shows the health status as follows: •...
Page 217 • Troubleshooting Firmware failure If a firmware failure occurred during operation of the module, an exception log has been created about the processes during the firmware failure. The firmware sends the exception log to the Instrument Audit Trail when the module is connected in the chromatography data system. In this case, observe the following: •...
Page 218 • Troubleshooting Messages The table lists the most frequently observed messages for the device and provides troubleshooting assistance. Each message consists of a code number and a text. The code number is the unique identifier for the problem while the wording may change. Note the following: •...
Page 219 • Troubleshooting TIP If you are unable to resolve the problem following the instructions in this manual, or if you encounter a message not listed in the table, write down the code and wording of the message and contact us. For details, see the Contacting Us section at the beginning of this manual.
Page 220 • Troubleshooting Message and code Description and remedial action Code 90 Download firmware You tried to download an incompatible firmware with an earlier mismatch – invalid version. version number than the firmware that is allowed by an internal downgrade barrier in the module. Downgrading the firmware may result in loss of functionality or malfunctioning of the module.
Page 221 • Troubleshooting Message and code Description and remedial action Code 126 Device lost system interlink All other modules on the system interlink bus are turned off or a connection. firmware download is performed on all other modules. 1. Check whether all other modules have been turned off or a firmware download is performed on all other modules on the bus.
Page 222 • Troubleshooting Message and code Description and remedial action Code 152 Assignment or command Module is not ready to accept command or property assignment rejected – device locked. since locked by another software (system controller or chromatography data system). Wait until module is released by the current software (system controller or chromatography data system).
Page 223 • Troubleshooting Message and code Description and remedial action Code 6106 Unexpected module The ion trap voltage is too low. Liquid may be present in the ion trap. behavior. The ion trap voltage is out The voltage may have been shorted due to detector flooding. of the expected range.
Page 224 • Troubleshooting Message and code Description and remedial action Code 6131 Data transfer error from • Check the USB connection. detector to PC – check USB • Try another, shorter, USB cable. Use only the USB cables provided connection. by Thermo Fisher Scientific for the detector. •...
Page 225 • Troubleshooting Message and code Description and remedial action Code 6200 Gas supply error. Check Gas supply to the detector may be too low or is turned off. gas supply, repeat self-test or cycle • Make sure that gas is supplied to the detector. power.
Page 226 • Troubleshooting Message and code Description and remedial action Code 6224 Assignment rejected in The detector was in error state when you tried to change a property. error state. Restart the self-test or • Repeat the self-test or turn off the module, wait for 5 seconds and power cycle detector.
Page 227 • Troubleshooting Operating issues This section gives an overview of possible operating issues and remedial actions. 8.3.1 Resolve liquid leaks When The leak sensor is wet. The leak sensor reports leakage. Parts and additional items required • Replacement part as required •...
Page 228 • Troubleshooting 2. With a cloth or tissue, thoroughly absorb all liquid that has collected in the leak tray and under the leak sensor. Be careful not to bend the sensor. 3. Allow the sensor to adjust to the ambient temperature for a few minutes.
Page 229 • Troubleshooting Follow these steps To remedy the situation, follow these steps: 1. Turn off the pump flow and then the gas flow: a) Turn off the pump flow to the detector. Wait at least 30 minutes. During the waiting period, the detector drains any remaining mobile phase to waste.
Page 230 • Troubleshooting 8.3.3 Resolve flooding in the detector When Liquid is visible in the gas exhaust tube due to build-up of excess liquid (flooding) inside the detector. Follow these steps 1. Leave the detector gas flow turned on for 24 hours to dry the excess liquid.
Page 231 • Troubleshooting Noise and/or poor precision Possible Cause Remedial Action Change in background An inexplicably changing background current signal can indicate a contaminated current solvent or system flow path. Make sure that the solvent and all components in the liquid flow path are free from contamination. Unstable evaporation If the evaporation tube temperature signal is unstable, this can lead to higher noise temperature...
Page 232 • Troubleshooting Loss of response Possible Cause Remedial Action Change in parameters Check that the detector is turned on and the data acquisition is on. Check the parameter settings. Gas flow turned off Check that the gas flow is turned on in the detector and the gas supply. Gas leaking Check the gas connections and the gas inlet and exhaust tubes.
Page 233 • Troubleshooting Liquid in the gas exhaust Possible Cause Remedial Action Flooding inside the Flooding can occur if the detector is turned off while liquid is still flowing into the detector (pump flow detector. turned on while no gas Resolve flooding in the detector (} page 230).
Page 234 • Troubleshooting Page 234 Charged Aerosol Detectors (VF-D21, VH-D21) Operating Manual...
Page 235 • Specifications 9 Specifications This chapter provides the physical and performance specifications, including information about the materials used in the flow path of the detector. For the related information for the display and the system controller, refer to the Vanquish User Interface Installation Guide. Charged Aerosol Detectors (VF-D21, VH-D21) Page 235 Operating Manual...
Page 236 • Specifications Detector performance specifications The detector performance is specified as follows: Type Specification Detection type Charged aerosol detection Nebulization FocusJet, concentric Pump flow rate VH-D21: VF-D21: 0.0 – 2.0 mL/min 0.2 – 2.0 mL/min Evaporation temperature VH-D21: VF-D21: Ambient (off), control settable Ambient (off), control settable to +35 °C, from +10 °C to +100 °C in 0.1 °C +50 °C and +70 °C...
Page 237 • Specifications Type Specification Solvent and additive Solvent and additive information (} page 29). information Safety features Power-up diagnostics of detector functions, online-monitoring of diagnostic parameters Gas inlet overpressure relief valve, fluid leak sensing with safe leak handling, high-temperature shutoff, flood prevention (pump flow shutoff, internal leak valve and diverter valve) Good Laboratory Practice Predictive Performance functions for scheduling maintenance procedures...
Page 238 • Specifications Physical specifications The physical conditions of the device are specified as follows: Type Specification Range of use Indoor use only Ambient operating 5 °C - 35 °C temperature Ambient storage temperature -20 °C - 45 °C Ambient operating humidity 20% - 80% relative humidity (non-condensing) Ambient storage humidity Maximum 60% relative humidity (non-condensing) Operating altitude...
Page 239 • Accessories, consumables and replacement parts 10 Accessories, consumables and replacement parts This chapter describes the standard accessories that are shipped with the device and the accessories that are available as an option. This chapter also provides information for reordering consumables and replacement parts.
Page 240 • Accessories, consumables and replacement parts 10.1 General information The device must be operated only with the replacement parts and additional components, options, and peripherals specifically authorized and qualified by Thermo Fisher Scientific. Accessories, consumables, and replacement parts are always maintained at the latest technical standard.
Page 241 • Accessories, consumables and replacement parts 10.2 Ship kit The ship kit includes the items listed in the table. The kit content is subject to change and may vary from the information in this manual. Refer to the content list included in the kit for the most recent information about the kit content at the time when the device is shipped.
Page 242 • Accessories, consumables and replacement parts 10.3 Optional accessories Item Part No. Corona nitrogen generator 1010A, 110 V 6084.8110A Corona nitrogen gas generator with integrated air compressor Provides on-demand, uninterrupted supply of dry nitrogen from a compressed air source. Corona nitrogen generator 1010A, 230 V 6084.8230A Corona nitrogen gas generator with integrated air compressor Provides on-demand, uninterrupted supply of dry nitrogen from a...
Page 243 • Accessories, consumables and replacement parts 10.4 Consumables and replacement parts 10.4.1 Capillaries, tubing and tubing tools For system capillaries, refer to the Vanquish System Operating Manual. Description Part No. Capillary kit, Viper, including: 6085.0300 • Viper Capillary, I.D. x length 0.10 x 150 mm, MP35N Connection diverter valve –...
Page 244 • Accessories, consumables and replacement parts 10.4.3 Interface cables and power cords Interface cables Description Part no. System interlink cable (RJ45), 0.5 m 6036.0004 USB cable, type A to type B, high-speed, USB 2.0 6035.9035A Cable length: 1 m USB cable, type A to type B, high-speed, USB 2.0 6911.0002A Cable length: 5 m Cable, mini-DIN, 6-pin, cable length: 5 m...
Page 245 • Accessories, consumables and replacement parts 10.4.5 Miscellaneous Description Part No. Front door kit, including right door and left door 6036.1920 Fuses kit, Vanquish system 6036.0002 The kit includes the appropriate fuses for the Vanquish system modules. For the detector, use only 3.15 AT, 250 V AC, slow-blow fuses.
Page 246 • Accessories, consumables and replacement parts Page 246 Charged Aerosol Detectors (VF-D21, VH-D21) Operating Manual...
Page 247 • Legal notices 11 Legal notices This topic provides copyright and trademark information. Charged Aerosol Detectors (VF-D21, VH-D21) Page 247 Operating Manual...
Page 248 • Legal notices 11.1 Copyright and trademarks Copyright © 2025 Thermo Fisher Scientific Inc. All rights reserved. Original Operating Manual Trademarks All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Acrobat, Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated.
Page 249 • Legal notices 11.2 Revision history Revision Covering VF-D21-A-01, VH-D21-A-01 The instructions were prepared in English (original instructions). Other language versions are translations based on the English original instructions. Charged Aerosol Detectors (VF-D21, VH-D21) Page 249 Operating Manual...
Page 250 • Legal notices Page 250 Charged Aerosol Detectors (VF-D21, VH-D21) Operating Manual...
Page 251 • Appendix 12 Appendix This chapter provides information about the use of the digital I/O ports. Charged Aerosol Detectors (VF-D21, VH-D21) Page 251 Operating Manual...
Page 252 • Appendix 12.1 Digital I/O ports The digital I/O ports are only available for the external configuration with analog output and the external configuration with digital output. The digital I/O ports (Dig I/O) can be used to exchange digital signals with external instruments, for example, a third-party LC system.
Page 253 • Appendix Signal name Remarks Input Function: Perform autozero The Autozero input can be used to set the signal of a channel to a zero point, regardless of the current that is being measured. Allow sufficient time after you performed the autozero and before injection to ensure that the autozero command is completed.
Page 254 • Appendix Figure 75: Digital I/O port 2 Signal name Remarks Input/output Not used Relay_NC Function: Turn off the pump flow (NC: normally closed) The Pump Off pin is part of a relay and can be used to connect to a desired signal voltage (high or low) which should be used as output signal on the Relay_COM pin to turn off the pump flow.
Page 255 • Appendix 12.1.3 Connect the digital I/O ports 1. Plug the 6-pin connector of the digital I/O cables of the third-party kit into the Dig I/O port 1 or 2 of the detector. 2. Connect the digital I/O cables to the external device. Refer to the Charged Aerosol Detector Third-Party Kit Installation Guide.
Page 256 • Appendix Page 256 Charged Aerosol Detectors (VF-D21, VH-D21) Operating Manual...
Page 257 Index Index parameters .......... 132 Predictive Performance ...... 169 accessories ............. 239 Smart Startup ........... 131 ship kit............ 241 Chromeleon parameters additives ............ 29 actual evaporation tube temperature.. 134 information .......... 29 actual nebulizer gas pressure .... 133 analog offset (VUI) ......... 140 autozero ........... 135 analog range (VUI).......... 140 data collection rate........
Page 258 Index device evaporation tube temperature coupling offset optimization .......... 146 (VUI)............ 138 preparing for operation ...... 123 evaporation tube temperature setpoint restart after long-term shutdown ..... 157 Chromeleon parameter ...... 134 shutdown .......... 154 set (VUI)............ 138 digital I/O............ 252 VUI ............ 142 pin assignment .......... 252 ExceptionLogClear (Chromeleon) ....
Page 259 Index guidelines introduction.......... 160 installation........... 58 predictive performance (Chromeleon).. 169 maintenance .......... 161 Predictive Performance (VUI).... 170 operation........... 111 safety guidelines........ 161 messages ............ 218 notification panel ........ 216 mobile phase guidelines ........ 118 injection valve move.............. 60 guidelines .......... 189 Mute Alarm............ 114 inspect............ 189 installation............ 57 capillaries and tubing ........ 87...
Page 260 Index optimization ........... 146 requirements data collection rate ........ 147 power cord .......... 63 guidelines .......... 146 system clearance ........ 62 parameters overview ........ 147 workbench.......... 62 overview (functional) ........ 33 restart device after long-term shutdown .. 157 return slide-in module ......... 211 RotorChanged.......... 169 parameters............. 132 peak width (Chromeleon) ...... 135 pH range............ 30...
Page 261 Index signal channel.......... 148 system equilibration ........ 131 background current........ 148 System Interlink......... 47, 75 background current (Chromeleon) ... 132 system module box evaporation tube temperature .... 149 evaporation tube temperature (VUI) .. 137 evaporation tube temperature (Chromeleon) signal 1 (VUI).......... 137 ............. 132 system stack signal channels dismount ..........
Page 262 Index configure the analog output board ... 140 display nebulizer gas pressure setpoint .. 138 diverter valve position ...... 139 download service data ...... 145 Emergency Mode ........ 140 evaporation tube temperature actual value ............. 137 evaporation tube temperature coupling offset ............ 138 evaporation tube temperature setpoint... 142 filter constant........ 140, 142 gas control.......... 138 nebulizer serial number ...... 136...
Page 263 Index Charged Aerosol Detectors (VF-D21, VH-D21) Page 263 Operating Manual...
Page 264 www.thermofisher.com Thermo Fisher Scientific Inc. 168 Third Avenue Waltham Massachusetts 02451...

This manual is also suitable for:

Vanquish vh-d21 Vanquish vfd21-a-01 Vanquish vh-d21-a-01

Thermo Scientific Vanquish VF-D21 Operating Manual

Quick Links

Need help?

Questions and answers

Related Manuals for Thermo Scientific Vanquish VF-D21

Summary of Contents for Thermo Scientific Vanquish VF-D21

This manual is also suitable for: