Shodex MSpak GF-4A Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex MSpak GF-4A is a pretreatment column for column
switching method. Removes proteins and rfactants in samples well by size exclusion mode and reversed phase
mode but is not suitable for trapping hydrophilic substances.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
F8700015 MSpak GF-4A
Base Material : Spherical porous particles of polyvinyl alcohol
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water
4. Usable Conditions
Maximum Flow Rate
Product Name
MSpak GF-4A
Usable solvents are listed below.
(1) The standard eluent is a mixture of water and acetonitrile or water and methanol of any ratio.
(2) Ethanol, Tetrahydrofuran (THF), Acetone, Ethyl acetate, N,N-dimethylformamide (DMF), and Chloroform can
also be used.
(3) Buffers and aqueous solutions of different salts can be used separately or together. Usable buffers include
phosphate, acetate, citrate, and Tris. Usable aqueous salt solutions include sodium chloride, potassium
chloride, sodium sulfate, potassium sulfate, and ammonium sulfate. Please keep total concentration of salts
under 0.5 M.
Operation Manual
Shodex MSpak GF-4A
Column Size (mm)
I.D.
4.6
Maximum Pressure
(mL/min) (MPa/column)
1.0
Resonac Corporation (https://www.shodex.com/)
Particle Size
(μm)
Length
10 9
pH Range
1.0 2 - 9
ver.20230323E
Theoretical Plate Number
(Per Column)
≥ 100
Temperature Range
(°C)
4 - 60
1