Shodex Mspak GF-310 4D Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex MSpak GF-310 4D is a polymer-based size exclusion
(SEC) chromatography columns with high solvent tolerance. In addition to the SEC analysis, it can be used for
LC/MS analysis of drugs in biological samples.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
F7600110 MSpak GF-310 4D
Base Material : Spherical porous particles of polyvinyl alcohol
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water
4. Usable Conditions
4.1 System Settings
Product Name
MSpak GF-310 4D
Usable solvents are listed below.
(1) The standard eluent is a mixture of water and acetonitrile or water and methanol of any ratio.
(2) Buffers and aqueous solutions of different salts can be used separately or together. Usable buffers include
phosphate, acetate, citrate, and Tris. Usable aqueous salt solutions include sodium chloride, potassium
chloride, sodium sulfate, potassium sulfate, and ammonium sulfate. Please keep total concentration of salts
under 0.1M.
Operation Manual
Shodex MSpak GF-310 4D
Column Size
Particle
(mm)
Size
(μm)
I.D. Length
4.6 150 5
Flow Rate (mL/min)
Recommended
0.2
Resonac Corporation (https://www.shodex.com/)
Theoretical
Target Molecular
Plate Number
(Per Column)
≥ 10,000
*1 Reference value only / Measured with pullulan
Maximum
Pressure
Maximum
(MPa/column)
0.5 4.0
ver.20221202E
Exclusion
Weight Range * Limit
300 - 30,000 40,000
pH Temperature
Range Range (°C)
2 - 9 4 - 60
*
1