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Isolera One
User manual
Biotage Isolera One User Manual
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Contents
Table of Contents
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Table of Contents
Table of Contents
1 : Description and Specifications
General System Description
Basic System Components
System Configurations
Programmed Functions
User Documentation
Accessories
Software Description
Main Menu
Data Administration Mode
System Mode
Chemistry Mode
Method Tab (Chemistry Mode)
Status Tab (Chemistry Mode)
Results Tab (Chemistry Mode)
Solvents Tab (Chemistry Mode)
Isolera Remote Viewer
Specifications
General System Specifications
Operational Performance Specifications
Solvent Specifications
Chemical Resistance of the Peristaltic Pump Tube (Isolera LS)
2 : Data Administration
Log into the Data Administration Mode
Administrate the Cartridge List
Add a Cartridge Type
Delete an Unused Cartridge Type
Edit a Cartridge Type
Select Cartridge Types to List
Administrate the Method List
Delete Methods
Export Methods
Import Methods
Set Default Methods
Administrate the Rack List
Add a Rack Type
Delete an Unused Rack Type
Edit a Rack Type
Select Rack Types to List
Administrate the Result List
Export and Delete Result Records
Clean up the System's Database
Administrate the Solvent List
Add a Solvent
Delete an Unused Solvent
Edit a Solvent
Select Solvents to List
Administrate the User List
Add a User Account
Change the Password or Privilege
Delete a User Account
Reset the Number of Performed Runs
3 : System Settings
Log into the System Mode
Change Detector Settings
Enable or Disable the Internal Detector
Connect and Enable an External Detector
Disable an External Detector
Enable or Disable the Leak Detector
Change General Settings
Set the Date and Time
Enable or Disable Mouse Pointer
Set the Pressure Unit
Set the Language Used in the Chemistry Mode
Enable the Λ-All Detection Mode and TLC to Step Gradient Editor
Configure a Network Connection
Change Report Settings
Set up a Printer and Auto Print of Reports
Set up File Sharing and Auto Save of Reports
Set up Auto Send of Reports
Change Runtime Settings
Enable or Disable Audible Alarm
Enable or Disable Automatic Rack Allocation
Enable or Disable Peak Mode
Enable or Disable Auto Extend
Enable or Disable Monitoring of Reservoirs
Enable or Disable Run Requirement Estimation
Specify How Flushes Are Performed
Configure Collection and Fractionation
Maintenance
Export the System Configuration and Logs
Restore the System Configuration
Back up and Restore the System's Database
Calibrate the Touch Screen
Calibrate the Fraction Collector
Calibrate the Internal Detector
Perform an Intensity Scan of the Internal Detector
Clean the Collect Valve
Release Stuck Check Valves
View and Reset the Usage Statistics
4 : Operation
Start up the System
Shut down the System
Control the UV Lamp
Create, Open, and Edit Methods
Create or Open a Method
Calculate Gradient from TLC Data
Create a Method Using the Method Wizard
Gradient Optimization
Create a Method Using a Web Browser
Prepare and Run a Purification
Prepare the System for a Run
Run a Purification
Change the Processing Order
Monitor and Control a Purification
Monitor the Purification in Progress
Bypass the Automatic UV Lamp Warm-Up Period
End Initial Waste and Start Collecting Fractions
Collect through the Waste Channel
Start and End an Isocratic Segment
Control a Manual Collection
Edit and Manually Extend a Purification
Pause, End, or Abort a Purification
Unload a Purification
Flush a Cartridge with Air
Purge a Cartridge
Access Result Records
Search for Records
View, Print, and Save Reports on a USB Memory Device or the Network
Save Records on a USB Memory Device or the Network
View System Status and Results from Your Office
Solvent and Waste Handling
Assign Solvents to the Solvent Inlets
Set the Reservoir Volumes
Prime the System
Access the Isolera Remote Viewer
5 : Maintenance
Contact Biotage 1-Point Support
Accessories
All Isolera Systems
Isolera Prime, Isolera One, and Isolera Four
Isolera LS
Spare Parts
Clean the Exterior of the System
Implement a Mobile Phase Change
Clean the Detector Flow Cell
Clean or Release Valves
Clean the Pump Check Valves
Release Stuck Check Valves
Clean the Collect Valve
Clean or Replace the Sample Loading Pump Tubing
Clean the Sample Loading Pump Tubing
Replace the Sample Loading Pump Tubing
Sonicate Solvent Inlet Filters
Leaks
Shut down the System
Internal Leakage
External Leakage
Assembling Tubes Correctly
Replace the Fuses
Replace the Needle
Replace the Detector Lamp(S)
6 : Troubleshooting
Contact Biotage 1-Point Support
Accessories and Spare Parts
Fraction Collector-Related Problems
Pump-Related Problems
Internal Detector-Related Problems
Gradient Problems
Leak Detected
Overpressure Detected
7 Manufacturer
Biotage Sweden AB
Sales Offices and Distributors
Technical Support
A: Collection and Fractionation
Collection and Fractionation Methods
Collection Methods
Fractionation Methods
Detector Signals
Possible Combinations
Collection and Fractionation Parameters
Shoulder Slope and Valley Fractionation
Fractionation Using the Shoulder Slope Parameter
Fractionation Using the Valley Slope Parameter
Collection and Fractionation Examples
Threshold Collection
Slope Collection
Slope Collection and Threshold Fractionation
Valley Fractionation
Shoulder Slope Fractionation
Auto Extend of Gradient
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General System Description
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Basic System Components
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