Ocean Optics Flame-T User Manual

Ocean Optics Flame-T User Manual

Miniature spectrometer
Table of Contents

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Flame Miniature Spectrometer
User Manual
Amy to r
For Products: FLAME-S, FLAME-T
Document: 225-00000-000-11-201604

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Table of Contents
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Summarization of Contents

Chapter 1: Introduction
Product Introduction
Introduces the Flame Spectrometer as the latest generation of Ocean Optics' Czerny-Turner design, highlighting its portability and flexibility.
Product Features
Details the key features of the Flame Spectrometer, including configurability, ease of use, and indicator LEDs.
Typical Applications
Lists various application areas for the Flame Spectrometer, such as life sciences, materials identification, and environmental monitoring.
Specifications Summary
Provides a summary of the key technical specifications for both FLAME-S and FLAME-T models.
Product Versions
Explains the existence of preconfigured and custom-configured Flame Spectrometer units and how to identify them.
Chapter 2: Installation and Setup
What’s In the Box
Lists the items included with the Flame Spectrometer package, such as the spectrometer, USB cable, and calibration data sheet.
Flame Installation
Provides general instructions for setting up and getting the new Flame spectrometer operational.
Software Installation
Details the process of installing the OceanView or SpectraSuite software for the Flame Spectrometer.
About OceanView
Provides an overview of OceanView software, its features, and compatibility with the Flame Spectrometer.
Hardware Setup
Explains how to connect the Flame Spectrometer to a computer via USB or serial port and its power requirements.
Ocean Optics Flame Fiber Optic Spectrometer Typical Set-up
Illustrates the typical setup for connecting the Flame Spectrometer to a computer using a USB port and accessories.
Hardware Features
Describes the key hardware features of the Flame Spectrometer, including indicator LEDs and slit interchangeability.
Change the Slit
Explains how to change the spectrometer's slit to match application requirements, detailing the procedure.
Accessories
Lists various cables and accessories available for connecting the Flame Spectrometer to other equipment.
DB15 Connector Cable (FLAME-CBL-DD4P-DB15P)
Details the DB15 connector cable used to connect the Flame to specific Ocean Optics accessories like light sources.
PAK50 Connector Cable (FLAME-CBL-DD4P-PAK50P)
Describes the PAK50 connector cable used to connect the Flame to the Breakout Box.
Breakout Box (HR4-BREAKOUT)
Explains the function of the Breakout Box in separating signals from the Flame's 40-pin connector for accessory use.
Interchangeable Slits
Discusses the capability of changing the Flame's slit size to match measurement needs.
Light Sources, Cuvette Holders and Other Accessories
Lists various accessories like fibers, light sources, and cuvettes available for use with the Flame Spectrometer.
Measurement Techniques – Typical Set-ups
Shows typical UV-Vis setups for basic spectroscopy techniques, including Absorbance.
Absorbance
Explains Absorbance measurement, comparing sample spectra to a reference, and its applications.
Common UV-Vis Applications
Lists common UV-Vis applications such as quantification of DNA & proteins and concentration measurements.
Reflectance & Transmission
Explains Reflectance and Transmission spectroscopy techniques, comparing reflected or transmitted light to a reference.
Fluorescence
Describes Fluorescence as a technique where emitted light at a higher wavelength is measured after sample excitation.
Irradiance
Explains Irradiance as measuring total energy of light at a given wavelength, relative or absolute.
Common Irradiance Applications
Lists common Irradiance applications such as measuring radiant output of lamps and LEDs and UV exposure.
Chapter 3: Flame Operation with OceanView
Overview
Outlines the information provided for performing basic data acquisition and saving with the Flame Spectrometer and OceanView software.
Launch OceanView
Explains how to launch the OceanView software, with instructions varying by operating system.
OceanView Main Screen
Describes the main OceanView screen and its various components for setting up, viewing, and saving acquisitions.
Connect the Flame in OceanView
Guides the user on how to connect the Flame Spectrometer to OceanView software, including rescanning for devices.
Set Acquisition Parameters
Explains how to set acquisition parameters like integration time and averaging within the Acquisition Group Window.
Quick View and Device Response
Describes the Quick View mode for displaying raw spectral data and explains device response and instrument response.
Continuous and Single Acquisitions
Details the controls for taking or pausing acquisitions, both individually and globally.
Save Data
Explains how to configure saving parameters, file types, directories, and naming conventions for data.
Saved Data Panel
Describes the Saved Data Panel for viewing, previewing, and adding overlays of saved spectral data.
Projects and Methods
Explains how OceanView allows saving and loading projects and methods for measurement setups.
Spectroscopy Application Wizards
Describes the use of Spectroscopy Application Wizards for setting up measurements through step-by-step guidance.
Dark and Reference Measurements
Explains the importance and process of Dark and Reference measurements for accurate spectroscopy.
Schematic View
Introduces the Schematic View as a graphical interface to visualize data flow from device to processed data.
Chapter 4: Troubleshooting
Overview
Offers answers to common questions and suggests consulting FAQs for troubleshooting the Flame Spectrometer.
Connecting Flame Before Software Installation
Provides steps to resolve issues when the Flame is connected before spectroscopy software is installed, depending on the OS.
Removing Unknown USB Devices
Details steps to remove an unknown device from Windows Device Manager to resolve driver installation issues.
Product Upgrades, Repairs and Servicing
Covers procedures for product upgrades, returning for repairs, and recommended servicing like recalibration.
Repairs
Outlines the procedure for returning the Ocean Optics Product for repair, including contacting representatives and packaging.
Servicing
Recommends yearly wavelength recalibration and lists the services offered by Ocean Optics.
Chapter 5: How the Flame Spectrometer Works
Overview
Provides an overview of the Flame spectrometer's operation from light entry to USB transmission and its configurations.
Interchangeable Slit
Explains the function of interchangeable slits as entrance apertures and lists available slit widths and their pixel resolution.
LongPass Absorbing Filter (optional)
Describes the optional absorbing filters, their function in limiting bandwidth, and lists available filter codes.
Collimating Mirror (specify Standard or SAG+)
Explains the role of the collimating mirror and the options for standard or SAG+ mirrors.
Grating and Wavelength Range
Details the grating installation process and lists available gratings with their intended use and spectral range.
Focusing Mirror (specify standard or SAG+)
Describes the focusing mirror that directs spectra to the detector plane and its available types.
Detector Collection Lens (optional)
Explains the function of the optional detector collection lens in enhancing light collection and reducing stray light.
Detector
Lists the two detector types available for the Flame (FLAME-S and FLAME-T) and their specifications.
OFLV Variable Longpass Order-sorting Filter (optional)
Details the function of order-sorting filters in blocking unwanted spectral orders and their combination with detector window upgrades.
Chapter 6: Technical Specifications
Mechanical Diagram
Provides outer dimensions of the Flame Spectrometer through a mechanical diagram.
Electrical Pinout
Details the pinout of the Flame's 40-pin Accessory Connector, listing function, voltage level, and description for each pin.
CCD Overview
Provides an overview of the Charge-Coupled Device (CCD) detector used in the Flame Spectrometer.
CCD Well Depth
Explains CCD well depth and its impact on signal-to-noise ratio, comparing large-well and small-well CCDs.
Signal Averaging
Discusses signal averaging as a technique to increase S:N and amplitude resolution in spectral measurements.
Internal Operation
Describes the internal operational aspects of the Flame Spectrometer, including pixel definition and data reading.
CCD Detector Reset Operation
Explains the CCD detector reset operation, including the time required for signal transfer and pixel reset.
Timing Signals
Details various timing signals used in the Flame Spectrometer, including strobe signals.
External Triggering
Explains how to trigger the Flame Spectrometer using various External Triggering options via the 40-pin Accessory Connector.
Triggering Modes
Details the Normal, External Synchronous, External Hardware Level, and External Hardware Edge trigger modes for data acquisition.
Chapter 7: Calibration
Overview
Provides information on performing wavelength and irradiance calibrations for the Flame Spectrometer.
Wavelength Calibration
Describes how to calibrate the wavelength of the spectrometer using spectral lines and polynomial equations.
Calibrating the Spectrometer Wavelength
Details the procedure for calibrating the spectrometer's wavelength using spectral lines and a spreadsheet program.
Saving the New Calibration Coefficients: USB Mode
Explains how to save new wavelength calibration coefficients onto the Flame's EEPROM memory chip using USB mode.
Irradiance Calibrations
Explains irradiance calibrations for quantifying spectra, differentiating between absolute and relative calibrations.
Chapter 8: Firmware and Advanced Communications
FLAME-S Firmware
Covers the hardware description, USB information, and instruction set for the FLAME-S firmware.
FLAME-T Firmware
Covers hardware description, spectral memory storage, USB info, and instruction set for FLAME-T.
Flame –T Serial Port Interface Communications and Control Information
Provides command information for controlling the FLAME-T via its RS-232 interface.

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