Ocean Optics NeoFox Installation And Operation Manual
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Ocean Optics NeoFox Installation And Operation Manual

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NeoFox Phase Fluorometer
Installation and Operation Manual
For Products: NeoFox-GT and NeoFox Sport
Document: 013-20000-009-02-201510

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Summary of Contents for Ocean Optics NeoFox

  • Page 1 NeoFox Phase Fluorometer Installation and Operation Manual For Products: NeoFox-GT and NeoFox Sport Document: 013-20000-009-02-201510...
  • Page 2 Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or...
  • Page 3 Important Safety Notices WARNINGS Intense UV radiation is emitted from the NeoFox LED. Do not look directly at the LED output with the naked eye once the SMA cap is removed. To verify there is power to the instrument, place a white piece of paper in front of LED 1.

  • Page 4: Table Of Contents

    Installing NeoFox Hardware Drivers ................10 Installing Hardware Drivers Using Windows Vista ..............10 Installing Hardware Drivers Using Windows XP................ 12 Chapter 3: Using NeoFox with NeoFox Viewer Software ....15 Overview ........................15 Entering a New Event ....................16 Data Logging .........................
  • Page 5 Appendix C: Analog Output .............. 47 Analog Output Connector ....................47 Configuring Analog Voltage Output Channel with NeoFox Viewer ........... 47 Configuring the Analog Current Output Channel with NeoFox Viewer ........48 Selecting a Data Source ......................49 Selecting a Data Range ......................50 Saving the Analog Output Settings ...................
  • Page 6 Table of Contents Analog Output Pinouts ....................51 Analog Output Parameters List ..................51 Analog Output Specifications ..................52 Interfacing to the 4-20mA Output ................... 52 4-20 mA Loop Voltage Supply ..................53 4-20 mA Isolation ......................54 Resistor / Volt Meter ...................... 54 Appendix D: Calculating Drift Per Sensor ........

  • Page 7: About This Manual

    About This Manual Document Purpose and Intended Audience This document provides the users of the NeoFox and NeoFox Sport with instructions for setting up, calibrating and performing experiments with their equipment. What’s New in this Document This version of the NeoFox Installation and Operation Manual updates the operation instructions.

  • Page 8: Safety And Compliance

    About This Manual  NeoFox Communication Interfaces Engineering Note Safety and Compliance This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.

  • Page 9: Chapter 1: Introduction

    Introduction NeoFox System Overview The NeoFox is a device for measuring fluorescence lifetime, phase and intensity. It uses LED excitation and photodiode detection with filter-based wavelength selection for easy experimental set-up and control. Because the unit is self-contained, it is invariant to fiber bending and stray light, and has a wide dynamic range of optical intensity as well as low optical and electronic crosstalk, and low drift and phase noise.

  • Page 10: Neofox Sport

    (limit of one sensor module per stack). Features NeoFox is a fluorescence-sensing detector for our optical sensors line and provides a viable alternative to traditional chemical sensing devices. Our optical sensors consist of transducer materials, applied to the tips of optical fibers or to substrates such as patches or cuvettes, which change optical properties in response to specific analytes in their immediate environment.

  • Page 11: Software

    Ocean Optics. The NeoFox connects to a PC via USB connection and saves your data in an easy-to-use Microsoft Excel format. NeoFox can be configured with single-channel LED excitation and detection, and modulation frequencies to 100 kHz.

  • Page 12: Neofox Sport

    It lists all items in the order, the shipping and billing addresses, and any items on back order. NeoFox Sport The following equipment and information ships with the NeoFox Sport: Jaz stack with the NeoFox Sport module(s) ...

  • Page 13: Chapter 2: Installation

    Do not connect the NeoFox unit to the PC prior to installing the NeoFox Viewer software. Software Installation You can install the NeoFox Viewer software from either the CD that came with your NeoFox unit or from the Ocean Optics website.
  • Page 14 Installing from the Web: d. Go to http://www.oceanoptics.com/technical/softwaredownloads.asp e. Right-click on NeoFox Viewer Software and select Save Target As… to download the executable to your machine. f. Click Save. The installation begins. g. Click Run. The NeoFox Viewer Setup screen appears.
  • Page 15 4. Click Next to select the default location, or browse to the desired location and click Next. You are now ready to install the software. 5. Click Install. The Neofox Viewer installs at the selected location. This process can take several minutes. When the software has successfully installed, the final Install Wizard screen appears.

  • Page 16: Hardware Installation

    Ensure that the plastic caps are covering the two SMA connectors on the front of the NeoFox unit. Intense UV radiation is emitted from the LEDS when the unit is powered-up. Do NOT look directly at the LED output with the naked eye.
  • Page 17 Components Additional Recommended Equipment). 2. Connect power cord from the power supply that came with your NeoFox unit from back of unit to an AC outlet. Do NOT look directly at the light being emitted with the naked eye. Caution Use only the power supply that came with your NeoFox unit.

  • Page 18: Installing Neofox Hardware Drivers

    Installing NeoFox Hardware Drivers When you connect NeoFox to a computer for the first time using the USB cable, the hardware drivers should install automatically for Windows 7, 8, 8.1 and 10 if you installed the NeoFox Viewer software first.
  • Page 19 4. Select Browse my computer for driver software (advanced). The next hardware wizard screen appears. 5. Highlight the driver_support directory (located beneath C:\Program Files\Ocean Optics\NeoFox- Viewer) and click OK followed by Next. The next hardware wizard screen appears. 6. If the following screen appears, select Install this driver anyway.

  • Page 20: Installing Hardware Drivers Using Windows Xp

    2: Installation Installing Hardware Drivers Using Windows XP The following Found New Hardware wizard appears when you first attach NeoFox to computers running the XP operating system. ► Procedure 1. From the first hardware wizard screen, select "No, not this time". Click Next. The next hardware wizard screen appears.
  • Page 21 2: Installation 2. Select “Install the software Automatically (Recommended).” Click Next. The system installs the necessary driver software to operate your NeoFox device. The next hardware wizard screen appears. 3. When the hardware driver installation completes successfully, the following screen appears:...
  • Page 22 2: Installation 013-20000-009-02-201510...

  • Page 23: Chapter 3: Using Neofox With Neofox Viewer Software

    Using NeoFox with NeoFox Viewer Software Overview When you first display the NeoFox Viewer software, the basic NeoFox Viewer screen appears. This screen’s controls enable you to perform the following actions:  Insert comments into the exported data file using the Enter New Event textbox. See...

  • Page 24: Entering A New Event

    Data Logging You can save a .csv file (useful for Microsoft Excel) via data logging. When you click the Start/Stop Data Logging button on the main NeoFox Viewer screen, the Data Logging Control Panel screen appears. When you click Start Logging, samples are exported from that point onward and continue until you click Stop Logging and then click Save Settings and Close Window.

  • Page 25: Advanced Settings

    3: Using NeoFox with NeoFox Viewer Software Note User-defined events entered in the Enter New Event field on the basic NeoFox Viewer screen are not written to the data logging export file. Field Description Start Logging Initiates data logging process. Actual data logging does not start until you click Save Settings and Close Window.

  • Page 26: Setup

    Manual Gain button ( ), the problem is likely that the firmware and software versions are not compatible. You will then need to install the appropriate software version onto your computer to use NeoFox. See Software Installation and Appendix B: Maintenance for more information.

  • Page 27: Status

    3: Using NeoFox with NeoFox Viewer Software Status Clicking on the Status tab displays the status information shown below. 013-20000-009-02-201510...

  • Page 28: Advanced Status

    3: Using NeoFox with NeoFox Viewer Software Advanced Status The Advanced Status tab displays the values of parameters such as Oxygen (base and converted), Tau, etc. as shown below. Advanced Settings This tab displays the advanced settings shown below for sensor waveform, decay waveform, FFT and salinity correction.
  • Page 29 3: Using NeoFox with NeoFox Viewer Software Setting Salinity Correction NeoFox Viewer provides you with the capability to change the salinity correction setting from its default setting of 0. Salinity correction applies to dissolved oxygen for PPM and µM/L; it does not apply to percentage.

  • Page 30: Sensor Type Selection

    3: Using NeoFox with NeoFox Viewer Software Sensor Type Selection Before using the software, you must select the appropriate sensor type from the three buttons in the Sensor field on the right side of the screen. Choose FOXY, HIOXY, or FOSPOR.

  • Page 31: Calibrating The Probe

    This calibration information is unique for each oxygen sensor probe itself, but is independent of the NeoFox hardware unit. Therefore, a new calibration must be loaded each time a new sensor is added or before first use.
  • Page 32 2. Click the Setup tab. The Setup screen appears. 3. Under Select Sensor Calibration Method, choose Multi-Point Calibration. 4. Under Select How Temperature Is Measured, choose Use NeoFox Temperature Sensor. 5. Under Enter Sensor Type And Optional Data, choose the type of sensor and add the serial number and any notes needed.

  • Page 33: Two-Point Calibration

    3: Using NeoFox with NeoFox Viewer Software 8. Click on button on the bottom right corner of the screen. The Computed Calibration Constants table fills in on the bottom left corner of the screen. 9. Click the button to use the coefficients for oxygen calculation. A Download Confirmation screen appears.
  • Page 34 3: Using NeoFox with NeoFox Viewer Software 3. Under Select Sensor Calibration Method, choose Two-Point Calibration. 4. Under Select How Temperature Will Be Measured, choose None (Two point calibration only). 5. Under Enter Sensor Type And Optional Data, choose the type of sensor and add the serial number and any notes needed.
  • Page 35 Compute Constants m and b. 13. To use this calibration for oxygen measurement, click the button. A Download Confirmation screen appears. 14. Click Yes to confirm the download and continue. The main NeoFox Viewer window will now display oxygen measurements. 013-20000-009-02-201510...

  • Page 36: Saved Settings

    3: Using NeoFox with NeoFox Viewer Software Note Normalized decay time = τ0/τ. Saved Settings The following parameters are saved onboard the NeoFox in its non-volatile memory. When the settings are saved, the saved values will be loaded when the device turns on: Measurement Parameters: ...
  • Page 37 3: Using NeoFox with NeoFox Viewer Software Calibration Settings:  Temperature Settings  Temperature Source  Fixed Temperature Value  Calibration Method  Sensor Type  Two Point Calibration Settings  Two Point – Tau0  Two Point – Tau1 ...

  • Page 38: Saving Data

    Saving Data You can save your NeoFox data in either a .fxy file, which can be opened in a NeoFox Viewer window or in a .csv file, which is compatible with Microsoft Excel. NeoFox saves data to a disk file until you request it to stop or the application is shut down.

  • Page 39: Neofox Communication Interfaces

    You may wish to write programs to interface directly to the NeoFox without using the NeoFox Viewer. There are a number of ways to do so: Through the NeoFox DLL, through the NeoFox RS232 port (only NeoFox-GT units with an F or later in the third digit of its serial number support RS232 communications), or through custom USB drivers.

  • Page 40: Resetting Saved Settings

    3: Using NeoFox with NeoFox Viewer Software 3. To save these settings through the NeoFox Sport, select Save Settings from the O2 Setup Menu. Note When the device saves a parameter through a flash write, it must save ALL of its savable settings at the same time.

  • Page 41: Chapter 4: Using Neofox Sport

    The NeoFox Sport is a field-portable extension of the NeoFox oxygen measurement system. Users of the NeoFox Sport have the ability to make real-time measurements in the field without a computer or external power supply. To do this, the NeoFox Sport system uses the same NeoFox measurement hardware as the desktop NeoFox system, but also includes a battery (to supply power) and a data processing unit (to provide a user interface).

  • Page 42: Neofox Sport Menu

    4: Using NeoFox Sport NeoFox Sport Menu Menu Tree Description 013-20000-009-02-201510...
  • Page 43 Enables you to view the sensed values for Time, O2, temperature and Tau. The Readout values displayed refresh every time a new sample is uploaded from NeoFox with the appropriate values. Displays the atmospheric pressure measured by the on-board pressure sensor on the NeoFox Sport.

  • Page 44: Setting Up The Neofox Sport

    2. Press the red power button ( ) to turn the device on. 3. Connect the USB cable between the USB port on the NeoFox module (not the DPU) and the computer. 4. Start the NeoFox Viewer software. See Chapter 3: Using NeoFox with NeoFox Viewer Software.

  • Page 45: Data Logging / Saving Data

    Logging Data The NeoFox Sport allows you to log data to an SD card via the Oxygen Sensor | Sensor Setup | Data Logging menu. When you select Turn datalog on from this menu, a log file is created on the SD card...

  • Page 46: Viewing The Data Log File Name

    <tab> tau <tab> algorithm pressure <tab> sensor pressure Where: Millisecond Count = The millisecond counter of the NeoFox device at the time of the actual sample. Because this value is stored at the actual sampling time, this value is a more reliable timing metric than the “upload date”...

  • Page 47: Neofox Sport Saved Settings

    NeoFox Sport Saved Settings Most of the parameters that can be set through the NeoFox Sport’s user menus can be saved to the NeoFox Sport module’s flash memory, which allows those settings to be restored the next time that the device is powered on.
  • Page 48 4: Using NeoFox Sport 013-20000-009-02-201510...

  • Page 49: Appendix A: Specifications

    Approximately 2.5W @ 5V Communications USB, 0-5V analog output; 4-20mA analog output. RS232 is available on the NeoFox GT model with an F or greater in the third digit of its serial number. NeoFox Sport Upload Interval The NeoFox Sport uploads data from the NeoFox module at least once per second in the Sport configuration.
  • Page 50 A: Specifications 013-20000-009-02-201510...

  • Page 51: Appendix B: Maintenance

    Updating Software You can overwrite the previous installation. ► Procedure 1. Download the installer for the latest version of the Neofox Viewer from the Ocean Optics Software Downloads page: www.oceanoptics.com/technical/softwaredownloads.asp 2. Unzip the directory and then run the “Setup.msi” installer wizard.
  • Page 52 After setting the display units to Previous software version. This problem was resolved in anything other than ppm, the version 1.92 of the Neofox Viewer. display will not return to percent Contact Ocean Optics if you see this oxygen. error in a more recent software version.
  • Page 53 HIOXY before 01 SEPT 2009, return it to probe’s automatic frequency KHz instead of 100 KHz. Ocean Optics for a full recalibration setting has been changed at 45 KHz. Or, you can use firmware HIOXY calibrations performed from 100 KHz to 45 KHz.
  • Page 54 B: Maintenance 013-20000-009-02-201510...

  • Page 55: Appendix C: Analog Output

     0-5V voltage source Analog threshold settings can be saved by the user to allow a NeoFox unit to operate in a stand alone mode, outputting analog signals without input from a computer. Configuring Analog Voltage Output Channel with NeoFox Viewer ►...

  • Page 56: Configuring The Analog Current Output Channel With Neofox Viewer

    Selecting a Data Range. You can save your settings by enabling the “Flash Write” setting momentarily. Configuring the Analog Current Output Channel with NeoFox Viewer ► Procedure 1. Select the Advanced Settings tab on the Advanced View screen to view the advanced settings.

  • Page 57: Selecting A Data Source

    You can save your settings by enabling the Flash Write setting momentarily. Selecting a Data Source There are six potential data sources that can be driven by the NeoFox. The pressure mode is not currently supported as an analog data source.

  • Page 58: Selecting A Data Range

    Selecting a Data Range The data ranges of the analog outputs are fully adjustable through the lower bound and upper bound settings in NeoFox Viewer. These settings are simply unitless values that are compared to the unitless value of the data source.

  • Page 59: Saving The Analog Output Settings

    View of Connector on Back of NeoFox Unit This is the view looking at the connector on the back of the NeoFox, with power plug on the right and the USB plug on the left. The connector is a standard PS-2 connector.

  • Page 60: Analog Output Specifications

    Interfacing to the 4-20mA Output The NeoFox includes a non-isolated 4-20 mA current sink that can be used to transmit oxygen, temperature, or tau data. It is extremely important that you set up the interface for this output correctly, or else irreparable damage to the NeoFox unit can occur.

  • Page 61: 4-20 Ma Loop Voltage Supply

    As a simple current sink, NeoFox does not provide its own power for the current loop. The user must provide an isolated power supply between 11 V and 30 V (measured at the input to the NeoFox unit) to power the loop. This power supply must be completely separate from the NeoFox and its power supply;...

  • Page 62: 4-20 Ma Isolation

    Some users (especially those in industrial settings) may need to use an isolated 4-20 mA loop. Unfortunately, the current sink on the NeoFox unit is not isolated and is not suitable for use in environments which may cause the loop voltage to exceed 30V. However, there are a number of third- party products available that can convert the output of the NeoFox into an isolated 4-20 mA loop transmitter.
  • Page 63 C: Analog Output 013-20000-009-02-201510...
  • Page 64 C: Analog Output 013-20000-009-02-201510...

  • Page 65: Appendix D: Calculating Drift Per Sensor

    Appendix D Calculating Drift Per Sensor Drift differs depending on the sensor formulation (FOXY, FOSPOR or HIOXY). See the table and example below. Stability (Continuous LED): FOXY Formulation FOSPOR Formulation HIOXY Formulation Lifetime Stability 0.0006 usec/ HR 0.003 usec/ HR 0.0002 usec/HR (Tau): Oxygen Stability %:...
  • Page 66 D: Calculating Drift Per Sensor 013-20000-009-02-201510...

  • Page 67: Index

    47 recommended, 4 analog voltage boundaries, 50 event APD voltage, 22 entering new, 16 applications, 3 features, 2 battery detector, 39 NeoFox Sport, 33 fiber, 4 firmware updating, 43 FOSPOR, 3 calibration, 4 FOXY, 2 multipoint, 23 probe, 23...
  • Page 68 Software and Resources Library CD, 4 features, 33 specifications, 41 menu, 34 Status tab, 19 menu description, 34 saved settings, 39 setup, 36 NeoFox Viewer, 3, 17 new event entering, 16 Advanced Status, 20 Setup, 18 Status, 19 troubleshooting, 43 oxygen sensor probes, 2, 4...

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