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Ocean Optics CHEM2000-UV-VIS Operating Manual And User Manual

Miniature fiber optic spectrophotometers
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Operating Manual

User's Guide
and
CHEM2000 and CHEM2000-UV-VIS
Miniature Fiber Optic Spectrophotometers
Ocean Optics, Inc.
380 Main Street
Dunedin, FL 34698
(727) 733-2447
(727) 733-3962 fax
For the latest information, consult our web site:
www.OceanOptics.com
Or, e-mail our Technical Service Department:
TechSupport@OceanOptics.com
021400

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Summary of Contents for Ocean Optics CHEM2000-UV-VIS

  • Page 1: Operating Manual

    Operating Manual User’s Guide CHEM2000 and CHEM2000-UV-VIS Miniature Fiber Optic Spectrophotometers Ocean Optics, Inc. 380 Main Street Dunedin, FL 34698 (727) 733-2447 (727) 733-3962 fax For the latest information, consult our web site: www.OceanOptics.com Or, e-mail our Technical Service Department: TechSupport@OceanOptics.com...
  • Page 2 Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or...

  • Page 3: Table Of Contents

    ................19 ISS-2 Integrated Sampling System ................... 19 µ µ µ µ 400- m Diameter Optical Fiber ..................22 CHEM2000-UV-VIS Sampling Components ..............23 ISS-UV-VIS Integrated Sampling System ................ 23 µ µ µ µ 300- m Solarization-resistant Optical Fiber ..............26...
  • Page 4 Experiment Tutorial ......................27 Absorbance Experiments ....................27 Transmission Experiments ....................28 Reflection Experiments ......................29 Relative Irradiance Experiments ..................30 Concentration Experiments ....................31 Kinetics Experiments ......................33 Appendix A: Changing the Settings on the PC2000 ..........34 Appendix B: PC2000 Pin-outs and Jumpers ..............

  • Page 5: Introduction

    So, they built their own. In 1992, the founders of Ocean Optics filled a substantial need in the research community and changed the science of spectroscopy forever by introducing a breakthrough technology: the S1000 Miniature Fiber Optic Spectrometer, nearly a thousand times smaller and ten times less expensive than previous systems.
  • Page 6 SMA terminations to the PC2000 and light source/sample holder. This silica-core, silica-clad µ fiber is 2 meters in length. For the CHEM2000-UV-VIS, the included optical fiber is a 300- m diamter solarization-resistant patch cord that carries light from the sample to the spectrometer.
  • Page 7 Wavelength Calibration Report to calibrate the wavelength of your spectrometer. Upgrades Customers sometimes find that they need Ocean Optics to make a change or an upgrade to their system. In order for Ocean Optics to make these changes, the customer must first contact us to obtain a Return...

  • Page 8: Quick Start

    Quick Start The CHEM2000 and CHEM2000-UV-VIS systems are easy to set up, allowing the user to start collecting data within minutes. This Quick Start provides brief instructions on installing your PC2000, installing and configuring the software, and setting up your system. To find detailed directions on a specific component, see the .
  • Page 9 SMA connector on the light source and screw the other end of the fiber into SMA connector on your PC2000, which you have already installed in your computer. If you have a CHEM2000-UV-VIS, take your µ...

  • Page 10: Installing The Pc2000 And Ooichem Software

    ADC1000 is a 12-bit, 8-channel, single-ended A/D card. This sturdy spectrometer-A/D card combination fits easily into an ISA-bus slot in the PC. The following directions apply to both CHEM2000 and CHEM2000-UV-VIS users. Each device in or connected to your computer is assigned specific settings; it’s similar to giving each device its own name so that your computer will know what to call and how to recognize the device.
  • Page 11 µ the SMA connector on your PC2000. If you have a CHEM2000-UV-VIS, take your 300- solarization-resistant fiber that came with your system and screw one end into the SMA connector on...
  • Page 12 Install OOIChem The resolution of your computer's monitor must be 800 x 600 or better to use OOIChem software. Before installing OOIChem, make sure that no other applications are running. Insert “Disk 1” into your floppy drive. (When prompted, install Disks 2 and 3.) Execute Setup.exe. At the “Welcome”...

  • Page 13: Using Ooichem Software

    Using OOIChem Software OOIC provides users with a real-time interface to a variety of acquisition, PERATING OFTWARE signal processing, display, and measurement functions. The 32-bit software program operates with Windows 95/98 and Windows NT and performs basic absorbance, emission and kinetics functions, which are presented in an easy-to-follow format that is ideal for undergraduate-level students and beginning spectroscopists.

  • Page 14: Display Functions

    OOIChem and it begins to acquire data, you see the raw voltage from the detector expressed in A/D counts. This spectral view mode is unique to Ocean Optics. It allows you to control signal processing functions before taking absorbance, transmission, and relative irradiance measurements. Be careful...

  • Page 15: Cursor Function Bar

    Relative Irradiance Selecting this mode switches the spectral window into Relative Irradiance Mode. The reference spectrum must be made in Scope Mode with a blackbody of known color temperature. (CHEM2000- UV-VIS users cannot make relative irradiance measurements because the light source that comes with the system is not a blackbody source with a known color temperature.) A dark spectrum is usually obtained by preventing light from entering the fiber that connects to the spectrometer.

  • Page 16: Text Box

    Cursor Properties In this bar, you can label the cursor and monitor the cursor’s X value and Y value. To the right of the X and Y values of the cursor is a cursor selection button that allows you to choose a cursor style and a point style.

  • Page 17: Subtract Dark

    Subtract Dark Selecting this box subtracts the current dark spectrum from the spectra being displayed. This command is useful if you are trying to look at a change in an emission spectrum or are trying to eliminate from the spectra fixed pattern noise caused by a very long integration period. The subtract dark spectrum function only acts on spectra displayed in Scope Mode.

  • Page 18: File Menu Functions

    File Menu Functions Save Spectral Values Select File | Save Spectral Values from the menu to save the current spectrum. Text box entries and acquisition parameters are included in the headers of these files. You can then use these files as overlays or import them into other software programs, such as Microsoft Excel.

  • Page 19: Edit Menu Functions

    Edit Menu Functions Clear Spectrum Overlays Select Edit | Clear Spectrum Overlays from the menu to remove static spectra from the graph. Clear Kinetics Values Select Edit | Clear Kinetics Values from the menu to clear both the kinetics values from the chart and to clear the kinetics traces.

  • Page 20: Spectrometer Menu Functions

    You would only select Spectrometer | Strobe Enable from the menu if you were operating an external strobe source. The CHEM2000 and CHEM2000-UV-VIS systems do not include a strobe light source. However, the ISS-UV-VIS that comes with the CHEM2000-UV-VIS can be turned off and on through the software and this function.
  • Page 21 This function allows you to enable or disable the triggering of external strobes through the spectrometer. You would only enter a value in this box if you were operating an external strobe source. The CHEM2000 and CHEM2000-UV-VIS systems do not include a strobe light source.

  • Page 22: Kinetics Configuration

    Spectrometer, A/D Converter Type, Base Address (I/O Range) and IRQ (Interrupt Request). The remaining hardware parameters are for other Ocean Optics systems. The parameters in this dialog box are usually set only once -- when OOIChem is first installed and the software first starts.

  • Page 23: Chem2000 Sampling Components

    CHEM2000 Sampling Components ISS-2 Integrated Sampling System The CHEM2000 comes with a light source/sample holder combination called the ISS-2 Integrated Sampling System. It is a fully integrated tungsten halogen light source and 1-cm square cuvette holder that couples to the PC2000 with optical fiber to create a small-footprint system from ~350 to 900 nm. The ISS-2 has a 900- hour bulb with a color temperature of 3100 Kelvin and has a 5-mm diameter f/2 collimating lens.
  • Page 24 Using the Filter Slots in the ISS-2 The filter slots (one at the light source, a second in the sample holder) can be used to hold filters or light blocks. The most useful filters include an FG-3 blue filter for increasing the relative energy near 400 nm and 800 nm compared to 600 nm, an IR cutoff filter to reduce stray light below 750 nm, and a 550 nm high-pass filter to eliminate second-order effects on Shortwave NIR measurements.
  • Page 25 ISS-2 Specifications Path length: 1 cm µ Collimating lens: BK 7 glass (~360 nm - 2 m*), 5 mm diameter, f/2 Collimating lens termination: SMA 905 Filter slot: accepts filters up to ¼" (6 mm) in thickness Base material: aluminum µ...

  • Page 26: 400- M Diameter Optical Fiber

    400-µ µ µ µ m Diameter Optical Fiber The 400-µm diameter optical fiber that comes with your CHEM2000 is a single-strand optical fiber. The active part of the fiber consists of a silica core, surrounded by a silica cladding material. The fiber is very fragile, and if not protected by a suitable buffer material, would be nearly useless for most applications.

  • Page 27: Chem2000-Uv-Vis Sampling Components

    5-mm diameter f/2 collimating lens to collect the light and funnel it to the solarization-resistant optical fiber that also comes with the CHEM2000-UV-VIS. The ISS-UV-VIS can be operated manually or through the software.
  • Page 28 2-3 of JB) to enable the tungsten bulb. Short pins 1-2 (that is, place a jumper over pins 1-2 of JB) to disable the tungsten bulb. Bulb Replacement The deuterium and tungsten bulbs cannot be replaced by unauthorized personal. To replace a bulb in the ISS-UV-VIS, contact Ocean Optics.
  • Page 29 Specifications Deuterium wavelength range: 200-400 nm Tungsten wavelength range: 360-850 nm Deuterium bulb life: 800 hours Tungsten bulb life: 2,000 hours Ignition delay: ~1.5 seconds to 60 seconds 0.3% in 30 minutes Peak-to-peak stability: 1.0% in 10 minutes Connector: SMA 905 Power input: 12 V Power requirement:...

  • Page 30: 300- M Solarization-Resistant Optical Fiber

    Other solarization-resistant fibers are hydrogen-loaded but their solarization-resistant properties do not last as the hydrogen eventually leaks out of the silica -- meaning that these hydrogen-doped solarization- resistant fibers have a “shelf life.” With Ocean Optics solarization-resistant optical fibers, there is no shelf life.

  • Page 31: Experiment Tutorial

    Now you are ready to take your measurements. Because of the components making up your CHEM2000 and CHEM2000-UV-VIS, your system is ideal for absorbance and transmission. The CHEM2000 can also make relative irradiance measurements. If, however, you wish to utilize your system for other measuring functions, additional products might be required.

  • Page 32: Transmission Experiments

    ! ! ! ! For transmission of light through solutions, we offer a transmission dip probe with screw-on, removable tips in 2-mm, 5-mm or 10-mm path lengths. Contact Ocean Optics for more information. To take a transmission measurement: Select Scope under Mode of Operation in the software display area. Make sure the signal is on scale.

  • Page 33: Reflection Experiments

    ! ! ! ! The components that came with the CHEM2000 and CHEM2000-UV-VIS will not allow the user to make reflection measurements. A reflection probe is necessary. We offer several components for reflection measurements such as a variety of reflection probes, a reflection probe holder, diffuse reflectance standards and an integrating sphere.

  • Page 34: Relative Irradiance Experiments

    ! ! ! ! The components that came with the CHEM2000-UV-VIS will not allow the user to make relative irradiance measurements. Only CHEM2000 users can perform relative irradiance measurements. In order to make relative irradiance measurement, the reference spectrum must be made in Scope Mode with a blackbody light source of known color temperature.

  • Page 35: Concentration Experiments

    Concentration Experiments The absorbance of a solution is related to the concentration of the species within it. The relationship, known as Beer’s Law, is: ε λ λ ε where A is the absorbance at wavelength λ, is the extinction coefficient of the absorbing species at wavelength λ, c is the concentration and is the optical pathlength.
  • Page 36 Click the Scan button or select Scan | Solution from the menu. The absorbance value will appear next to the concentration for Solution #1. At any point, you can select Edit | Clear from the menu to clear the dialog box of all data. Take Solution #1 out of the cuvette holder and put in another standard solution with the next highest known concentration.

  • Page 37: Kinetics Experiments

    Kinetics Experiments Select Spectrometer | Kinetics Configuration from the menu to configure and establish the parameters for a kinetics experiment. In the Kinetics Configuration dialog box, you can collect spectral data as a function of time, from up to 4 single wavelengths and up to two mathematical combinations of these wavelengths. A kinetics experiment will not be displayed in the graph unless you choose Spectrometer |...

  • Page 38: Appendix A: Changing The Settings On The Pc2000

    Appendix A: Changing the Settings on the PC2000 Base Address Settings To change the Base Address settings on the PC2000, find the bank of switches on the A/D converter. The Base Address may be changed via the first 6 switches (the IRQ may be changed via the last 3 switches). Switches in the OFF position have the decimal values shown.
  • Page 39 Interrupt Request Settings To change the IRQ settings on the PC2000, see the bank of switches on the A/D converter. The IRQ may be changed via the last 3 switches. The following matrix defines the different IRQ settings by switch positions 7, 8, and 9.

  • Page 40: Appendix B: Pc2000 Pin-Outs And Jumpers

    Appendix B: PC2000 Pin-outs and Jumpers It is not necessary for the average user to worry about the interconnect scheme, as the cables supplied with all of the units need only be plugged into the matching connectors on the hardware. However, if the need arises to design and fabricate your own cabling system, the following tables supply the necessary information.

  • Page 41: Appendix C: Calibrating The Wavelength Of Your Spectrometer

    Your Spectrometer The following describes how to calibrate the wavelength of your spectrometer. Though each spectrometer is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions. You are going to be solving the following equation, which shows that the relationship between pixel number and wavelength is a third-order polynomial .
  • Page 42 Independent Dependent Values computed from Variable Variables the regression output True Predicted Wavelength Difference Pixel # Pixel # Pixel # Wavelength (nm) 253.65 30625 5359375 253.56 0.09 296.73 87616 25934336 296.72 0.01 302.15 97344 30371328 302.40 -0.25 313.16 116964 40001688 313.02 0.13 334.15...

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