Ocean Optics NIR Series Installation And Operation Manual
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Ocean Optics NIR Series Installation And Operation Manual

Near infrared fiber optic spectrometers
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NIR Near Infrared
Fiber Optic Spectrometers
Installation and Operation Manual
Document Number 197-00000-512-02-0305
Offices:
Ocean Optics, Inc.
830 Douglas Ave., Dunedin, FL, USA 34698
Phone 727.733.2447
Fax
727.733.3962
8:30 a.m.-6 p.m. EST
Ocean Optics B.V. (Europe)
Nieuwgraaf 108 G, 6921 RK DUIVEN, The Netherlands
Phone 31-(0)26-3190500
F ax
31-(0)26-3190505
E-mail:
Info@OceanOptics.com
Info@OceanOpticsBV.com
Orders@OceanOptics.com
TechSupport@OceanOptics.com (Technical support)
(General sales inquiries)
(European sales inquiries)
(Questions about orders)

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  • Page 1 Document Number 197-00000-512-02-0305 Offices: Ocean Optics, Inc. 830 Douglas Ave., Dunedin, FL, USA 34698 Phone 727.733.2447 727.733.3962 8:30 a.m.-6 p.m. EST Ocean Optics B.V. (Europe) Nieuwgraaf 108 G, 6921 RK DUIVEN, The Netherlands Phone 31-(0)26-3190500 F ax 31-(0)26-3190505 E-mail: Info@OceanOptics.com (General sales inquiries) Info@OceanOpticsBV.com...
  • Page 2 Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the information contained in this manual.

  • Page 3: Table Of Contents

    Table of Contents About This Manual ......................iii Document Purpose and Intended Audience ................iii What’s New in this Document ....................iii Document Summary........................iii Product-Related Documentation ....................iv Upgrades ......................... iv Chapter 1: Introduction ..............1 Product Description ......................1 About the Modular Approach.....................
  • Page 4 Table of Contents Chapter 4: Sample Experiments ............17 Preparing for Experiments....................17 Taking Measurements ..................... 18 Application Tips ......................... 18 Absorbance Experiments ......................19 Transmission Experiments ......................21 Reflection Experiments ......................23 Relative Irradiance Experiments ....................25 Time Acquisition Experiments ....................27 Appendix A: Calibrating the Wavelength of the NIR Spectrometer About Wavelength Calibration ..................

  • Page 5: About This Manual

    About This Manual Document Purpose and Intended Audience This document provides you with instructions to get your system up and running. In addition to the NIR Spectrometer installation and operation instructions, this manual also includes information for locating Product-Related Documentation the OOIBase32 installation instructions (see below).

  • Page 6: Product-Related Documentation

    Engineering-level documentation is located on our website at Technical → Engineering Docs. Upgrades Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system. To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise Authorization (RMA) number.

  • Page 7: Chapter 1: Introduction

    “hot-swapping” of spectrometers among multiple PCs. As with all other Ocean Optics user-configured spectrometers, you can choose from six slit sizes and hundreds of fiber optic accessories (such as light sources, probes, and optical fibers) to create the system just right for your application.

  • Page 8: About The Modular Approach

    Ocean Optics also offers a complete line of spectroscopic accessories to use with the NIR Spectrometer. Most spectroscopic accessories have SMA connectors for application flexibility. Thus, changing the sampling system is as easy as unscrewing a connector and adding a new component or accessory.

  • Page 9: Items Included With Shipment

    Please save the Wavelength Calibration Data Sheet for future reference. Software and Resources Library CD – Each spectrometer order comes with Ocean Optics’ Software and Resources Library CD. This disc contains all Ocean Optics software and manuals for software operation, spectrometers, and spectroscopic accessories. Documentation is provided in Portable Document Format (PDF).
  • Page 10 1: Introduction 197-00000-512-02-0305...

  • Page 11: Chapter 2: Installing The Nir Spectrometer

    Chapter 2 Installing the NIR Spectrometer Overview You must install the OOIBase32 software application prior to connecting the NIR Spectrometer to the PC. The OOIBase32 software installation installs the drivers required for NIR Spectrometer installation. If you do not install OOIBase32 first, the system will not properly recognize the NIR Spectrometer. If you have already connected the NIR Spectrometer to the PC prior to installing OOIBase32, consult Troubleshooting Chapter 3:...

  • Page 12: Serial Port Mode

    2: Installation If OOIBase32 was installed prior to connecting the NIR Spectrometer, the Add New Hardware Wizard appears and installs the NIR Spectrometer drivers. If the drivers do not successfully install, or if you connected the NIR Spectrometer to the PC before installing OOIBase32, consult Chapter 4: Troubleshooting.

  • Page 13: Configuring The Nir Spectrometer In Ooibase32

    2: Installation Configuring the NIR Spectrometer in OOIBase32 Once the NIR Spectrometer is installed, you must configure OOIBase32’s Configure Spectrometer options so that the application recognizes the connected NIR Spectrometer. The following sections contain instructions on initially configuring the NIR Spectrometer the first time you start OOIBase32.

  • Page 14: Configure Hardware Screen

    2: Installation Configure Hardware Screen The Configure Hardware screen prompts you to enter spectrometer-specific information into OOIBase32 the first time you run the program. Typically, you need only enter this information the first time you run OOIBase32. However, you can alter the hardware configuration at any time using the Spectrometer Configuration screen.

  • Page 15: Spectrometer Configuration Screen

    2: Installation 3. Select the COM port that NIR Spectrometer is connected to in the Serial Port drop-down menu. Troubleshooting See Chapter 3: for information on identifying serial ports. 4. Select the NIR Spectrometer’s operating speed from the Baud Rate drop-down menu (115,200 baud is recommended).

  • Page 16: Connect Spectroscopic Accessories

    Connect Spectroscopic Accessories To find operating instructions on NIR Spectrometer-compatible products such as light sources, sampling chambers, probes, fibers or any other Ocean Optics spectroscopic accessories, check the Software and Technical Resources CD or the Ocean Optics website at: http://www.oceanoptics.com/Technical/OperatingInstructions.asp.
  • Page 17 2: Installation Control Description TEC On Check this box to enable (recommended setting for laboratory conditions) or uncheck to disable the thermoelectric cooler. Det. Temp. Set Point Enter the target temperature of the NIR Spectrometer detector as follows: • NIR512: Minimum is –10 ºC, Recommended is –5 ºC •...
  • Page 18 2: Installation 197-00000-512-02-0305...

  • Page 19: Chapter 3: Troubleshooting

    NIR Spectrometer Connected to PC Prior to OOIBase32 Installation If you connected your NIR Spectrometer to the computer prior to installing your Ocean Optics software application, you may encounter installation issues that you must correct before your Ocean Optics device will operate properly.

  • Page 20: Remove The Unknown Device From Windows Device Manager

    3: Troubleshooting Remove the Unknown Device from Windows Device Manager ► Procedure 1. Open Windows Device Manager. Consult the Windows operating instructions for your computer for directions, if needed. 2. Locate the Other Devices option and expand the Other Devices selection by clicking on the "+" sign to the immediate left.

  • Page 21: Troubleshooting The Serial Port Configuration

    4. Navigate to the Windows | System32 | Drivers directory. 5. Delete the EZUSB.SYS file. 6. Reinstall your Ocean Optics application and reboot the system when prompted. 7. Plug in the USB device. The system is now able to locate and install the correct drivers for the USB device.

  • Page 22: Old Version Of Ooibase32 Installed

    If the PC to be used to interface to your NIR Spectrometer already has an older version of OOIBase32 software installed, you must install the latest version of OOIBase32. You can download the latest version of OOIBase32 from the Software and Technical Resources CD or from the Ocean Optics website at http://www.oceanoptics.com/technical/softwaredownloads.asp.

  • Page 23: Chapter 4: Sample Experiments

    Chapter 4 Sample Experiments The following sections contain information on conducting sample experiments using the NIR Spectrometer and OOIBase32. Preparing for Experiments ► Procedure Follow the steps below to configure the NIR Spectrometer and OOIBase32 for experiments: 1. Double-check that you have correctly installed the NIR Spectrometer, installed OOIBase32, and configured the light source and other sampling optics.

  • Page 24: Taking Measurements

    4: Sample Experiments Once you installed and configured your hardware and software and have set up your system, you are ready to take measurements. There are four basic optical measurements from which to choose: absorbance, transmission, reflection, and relative irradiance measurements. The type of measurements you wish to make determines the configuration of the sampling optics for your system.

  • Page 25: Absorbance Experiments

    4: Sample Experiments Absorbance Experiments Absorbance spectra are a measure of how much light is absorbed by a sample. For most samples, absorbance is linearly related to the concentration of the substance. The software calculates absorbance (Al) using the following equation: λ...
  • Page 26 4: Sample Experiments ► Procedure Follow the steps below to take an absorbance measurement using OOIBase32: 1. Place OOIBase32 in scope mode by either clicking the scope mode icon on the toolbar, or selecting Spectrum | Scope Mode from the menu. 2.

  • Page 27: Transmission Experiments

    4: Sample Experiments Transmission Experiments Transmission is the percentage of energy passing through a sample relative to the amount that passes through the reference. Transmission Mode is also used to show the portion of light reflected from a sample. Transmission and reflection measurements require the same mathematical calculations. The transmission is expressed as a percentage (%T ) relative to a standard substance (such as air).
  • Page 28 4: Sample Experiments 2. Verify that the signal is on scale. The peak intensity of the reference signal should be about 50,000 counts. 3. Ensure that nothing is blocking the light path from the sample to the spectrometer, and then take a reference spectrum.

  • Page 29: Reflection Experiments

    4: Sample Experiments Reflection Experiments Reflection is the return of radiation by a surface, without a change in wavelength and can be: • Specular, in which the angle of incidence is equal to the angle of reflection. • Diffuse, in which the angle of incidence is not equal to the angle of reflection. Every surface returns both specular and diffuse reflections.
  • Page 30 4: Sample Experiments ► Procedure Perform the following steps to take a reflection measurement using OOIBase32: 1. Verify that OOIBase32 is in scope mode by clicking the scope mode icon on the toolbar, or by selecting Spectrum | Scope Mode from the menu bar. 2.

  • Page 31: Relative Irradiance Experiments

    4: Sample Experiments Relative Irradiance Experiments Irradiance is the amount of energy at each wavelength from a radiant sample. In relative terms, it is the fraction of energy from the sample compared to the energy collected from a lamp with a blackbody energy distribution, normalized to one at the energy maximum.
  • Page 32 4: Sample Experiments ► Procedure Perform the following steps to take a relative irradiance measurement using OOIBase32: 1. Verify that OOIBase32 is in scope mode by clicking the scope mode icon on the toolbar, or by selecting Spectrum | Scope Mode from the menu bar. 2.

  • Page 33: Time Acquisition Experiments

    4: Sample Experiments Note If at any time any sampling variable changes (including integration time, averaging, boxcar smoothing, etc.) you must store a new reference and dark spectrum. Time Acquisition Experiments OOIBase32 allows you to perform time acquisition experiments. Time acquisition experiments track processes, perform kinetic analyses, and monitor spectral events all as a function of time.
  • Page 34 4: Sample Experiments Configuring the Time Acquisition Channel Configuration Screen ► Procedure Perform the following steps on the Time Acquisition Channel Configuration screen: 1. Select Enabled to set the time acquisition calculation for the wavelength. The time acquisition process will not calculate data if you do not select this option for at least 2.
  • Page 35 4: Sample Experiments Configuring for a Combination of Two Time Channels Configure a time acquisition process for a combination of two time channels (if desired) by selecting the Combination 1 tab on the Time Acquisition Channel Configuration screen. ► Procedure Perform the steps below to configure a combination: 1.
  • Page 36 4: Sample Experiments 2. Enable Stream Data to Disk to save time acquisition data. 3. Enter a value in the Write Data to Disk Every x Acquisitions box to set the frequency for saving data. OOIBase32 saves data more frequently if the number is smaller, or less frequently if the number is larger.
  • Page 37 4: Sample Experiments Note OOIBase32 has options to either store data for each acquisition, or to collect data only after a specified delay. Several factors affect the minimum time acquisition frequency, including integration time, number of spectrometer channels, samples averaged, and computer speed.
  • Page 38 4: Sample Experiments 197-00000-512-02-0305...

  • Page 39: Appendix A: Calibrating The Wavelength Of The Nir Spectrometer

    NIR Spectrometer This Appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions.

  • Page 40: Calibrating The Spectrometer

    A light source capable of producing spectral lines. Ocean Optics’ AR-1 Argon lamp or HG-1 Mercury-Argon lamp are ideal for this purpose. If you do not have an AR-1 or HG-1, you will need a spectral line source that produces several (at least 4-6) spectral lines in the wavelength region of your spectrometer.
  • Page 41 A: Calibrating the Wavelength of the NIR Spectrometer Independent Dependent Values computed from Variable Variables the regression output Predicted True Wavelength (nm) Pixel # Pixel # Pixel # Difference Wavelength 253.65 30625 5359375 253.56 0.09 296.73 87616 25934336 296.72 0.01 302.15 97344 30371328...

  • Page 42: Saving The New Calibration Coefficients: Usb Mode

    A: Calibrating the Wavelength of the Spectrometer 7. Record the Intercept as well as the First, Second, and Third Coefficients. Also, look at the value for R squared. It should be very close to one. If it is not, you have probably assigned one of your wavelengths incorrectly.

  • Page 43: Saving The New Calibration Coefficients: Serial Mode

    A: Calibrating the Wavelength of the NIR Spectrometer 12. Repeat Step 12 for all of the new values. 13. Click on the Save All Values button to save the information, and then Exit the USB Programmer software. The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the NIR Spectrometer.
  • Page 44 A: Calibrating the Wavelength of the Spectrometer 197-00000-512-02-0305...

  • Page 45: Appendix B: Specifications

    Entrance slits are rectangular apertures, with the width determining the amount of light entering the optical bench. A slit is permanent and can only be changed by an Ocean Optics technician. Slit* You can also use the NIR Spectrometer without a slit installed. In this case, the diameter of the fiber connected to the NIR Spectrometer determines the size of the entrance aperture.

  • Page 46: Nir Detector Specifications

    B: Specifications Name Description Each pixel on the detector responds to the wavelength of light that strikes it. InGaAs Detector Electronics bring the complete spectrum to the software. NIR Detector Specifications NIR512 NIR256-2.1 NIR256-2.5 Hamamatsu G9204 512- Hamamatsu G9206 256- Hamamatsu G9208 256- element InGaAs cooled element InGaAs cooled...

  • Page 47: Nir Spectrometer - Slit Size Vs. Resolution And Throughput

    B: Specifications NIR Spectrometer – Slit Size vs. Resolution and Throughput The following chart illustrates the effect that varying slit sizes have on NIR512 Spectrometer resolution and throughput: Slit Size (microns) NIR Spectrometer Specifications NIR512 NIR256-2.1 NIR256-2.5 Dimensions 105 mm x 127 mm x 80 mm Weight 190 g (without cable) Power...
  • Page 48 B: Specifications NIR512 NIR256-2.1 NIR256-2.5 Optical ~3.0 - 4.0 nm FWHM ~12.0 nm FWHM resolution Focal length f/4, 40mm Dynamic range 5000:1 for a single scan Fiber optic SMA 905 to single-strand optical fiber (0.22 NA) connector A/D converter: 16-bit internal - 500 KHz Data transfer Full scans into memory every 10 milliseconds (USB interface) rate...

  • Page 49: Compatibility For Desktop Or Notebook Pcs

    B: Specifications Grating Efficiency Curves Compatibility for Desktop or Notebook PCs To use the NIR Spectrometer with your PC, the computer must meet the following requirements: • IBM-compatible PC with Pentium or better microprocessor • 32 MB RAM • OOIBase32™ 32-bit Spectrometer Operating Software •...

  • Page 50: 9-Pin Rs-232 Serial Port Connector - Pin Definitions

    B: Specifications 9-pin RS-232 Serial Port Connector – Pin Definitions Pin # Description RS232 Transmit RS232 Receive Ground 15-pin Accessory Connector Pinout When facing the 9-pin RS-232 Serial Port connector on the NIR Spectrometer, the pins are numbered as: 15-pin Accessory Connector – Pin Definitions Pin # Description +5V DC...

  • Page 51: Appendix C: Nir256-2.5Μm Sensitivity

    Appendix C NIR256-2.5µm Sensitivity When configuring a system for operation out to 2.5µm it is important that you consider the following details: • Short Integration Times: For the detector to be sensitive out to 2.5µm, the detector’s band gap energy must be small. Unfortunately, this raises the absolute level of the detectors dark signal. A typical dark signal at 34ms is shown below.
  • Page 52 C: NIR256-2.5 µm Sensitivity The figure below shows the relative intensity of a NIR256-2.5 Spectrometer with a 25um slit directly coupled to an LS-1 Light Source with a 600um fiber. 197-00000-512-02-0305...

  • Page 53: Index

    B: Specifications Index Accessories, 10 Grating, 39 Adobe Acrobat Reader, 3 grating efficiency, 43 Calibrating, 33 Installation calibration coefficients Serial, 6 saving in Serial mode, 37 USB, 5 CCD Detector, 40 Installed Filter, 39 Compatibility, 43 Installing, 5 Configure Hardware, 8 Serial Port mode, 8 USB mode, 8 LED, 44...
  • Page 54 Index range, 41 Time Acquisition, 27 resolution, 42 Troubleshooting, 13 serial number, 7 upgrades, iv Slit, 39 USB-ADP-PC, 2 SMA Connector, 39 USB-CBL-1, 2 Software and Resources Library CD, 3 Specifications Detector, 40 Wavelength Calibration, 33 Spectrometer, 41 Wavelength Calibration Data File, 3 spectral response, 42 Wavelength Calibration Data Sheet, 3 Spectrometer Configuration Screen, 9...

This manual is also suitable for:

Nir256-2.1Nir512Nir256-2.5

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