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Gebrauchsanleitung
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Instrucciones de manejo
Руководство по эксплуатации
Használati utasítás
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Návod kpoužití
Instrukcja użytkowania
Operating manual
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Mode d'emploi
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Istruzione
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Instruções de utilização
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사용 지침
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Gebruiksaanwijzing
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Kullanım Talimatları
seripettor®
seripettor® pro
Flaschenaufsatzdispenser | Bottle-top dispenser
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操作手册
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Bruksanvisning
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使用説明書
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Table of Contents
Related Manuals for BRAND seripettor
Summary of Contents for BRAND seripettor
- Page 1 Gebrauchsanleitung Operating manual Mode d'emploi Instrucciones de manejo Istruzione Instruções de utilização 操作手册 Руководство по эксплуатации 사용 지침 Bruksanvisning 使用説明書 Használati utasítás Návod kpoužití Gebruiksaanwijzing Instrukcja użytkowania Kullanım Talimatları seripettor® seripettor® pro Flaschenaufsatzdispenser | Bottle-top dispenser...
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Page 2: General Safety Instructions
(e.g., piston difficult to move, sticking valves or leak- age), immediately stop dispensing and consult Troubleshooting. Contact the manufacturer, if necessary. 2.2 Function The seripettor® bottle-top dispenser is used for dispensing liquids directly from the storage bottle and is available in two versions: seripettor® seripettor® pro... -
Page 3: Limitations Of Use
2.3 Limitations of Use This instrument is designed for dispensing liquids, observing the following physical limits: • Use between +15°C and +40°C of instrument and reagent (seripettor®: agar culture medium up to max. 60 °C) • Vapor pressure up to 500 mbar •... -
Page 4: Operating Exclusions
Concentrated or oxidizing acids • Explosive liquids • Carbon disulfide 2.5.2 seripettor® pro Never use seripettor® pro for: • Liquids attacking PP, PE, Al -ceramic, ETFE, FEP, PFA and PTFE (e.g., dissolved sodium azide*) • Liquids attacking borosilicate glass (e.g., hydrofluoric acid) •... - Page 5 Polar solvents E.g., ethanol, methanol, acetylacetone, etc. 2.7.2 seripettor® pro application range The seripettor® pro bottle-top dispenser expands the range of applications. It enables dispensing of acids (e.g., concentrated HCl), polar solvents (e.g., acetone) and UV-sensitive media 997468 Operating manual...
- Page 6 Ethyl methyl ketone — ✓ Formaldehyde, 40% ✓ ✓ Not suitable for hydrofluoric acid (HF) Formamide ✓ ✓ seripettor® and seripettor® pro are not suitable Glycol (ethylene glycol) ✓ ✓ for dosing hydrofuoric acid (HF). Glycolic acid, 50% ✓ ✓ Glycerin ✓...
- Page 7 ✓ tures can be dispensed. If you need chemical Zinc chloride, 10% ✓ ✓ hazard statements that are not on the list, you Zinc sulfate, 10% ✓ ✓ are welcome to contact BRAND. Legend: Last updated: 0522/10 997468 Operating manual...
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Page 8: Functional And Operating Elements
3 Functional and operating elements seripettor® and seripettor® pro 1 Actuating unit 2 Piston locking mecha- nism 3 Volume adjustment 4 Dispensing cartridge (piston/cylinder) 5 Valve block 6 Valve block adapter (GL 45 bottle thread) 7 Filling tube 8 Discharge tube with... -
Page 9: First Steps
4 Assembly 4 Assembly 4.1 First Steps WARNING Follow during every use, especially with hazardous media > Wear protective clothing, eye protection and appropriate hand protection! > Never press down the piston when the screw cap is screwed on! > Avoid splashing the reagent! >... - Page 10 Check that the discharge tube is securely seated. Re-tighten cap nut(s) after two days of use. 3. Installing the filling tube a. seripettor®: Cut the filling tube off according to the bot- tle height and attach it. a'. seripettor® pro: Adjust the length of the telescopic filling tube to the bottle height and attach it.
- Page 11 4 Assembly 4. Installing the instrument on a bottle Screw the instrument (GL 45 threads) onto the reagent bottle. To avoid tipping over, use a bottle stand for small bot- tles. NOTICE For bottles with other thread sizes, select a suitable adapter, see Accessories/spare parts, p. 79 5.
- Page 12 4 Assembly 6. Priming the instrument Hold the discharge tube and remove the closure cap/ screw cap. To avoid splashes, hold discharge tube orifice on the in- ner wall of a suitable receiving vessel. For priming, let the piston move up approx. 30 mm and push it down rapidly until the lower stop.
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Page 13: Operation
5 Operation 5 Operation 5.1 Dispensing WARNING Follow during every use, especially with hazardous media > Wear protective clothing, eye protection and appropriate hand protection! > Never press down the piston when the screw cap is screwed on! > Avoid splashing the reagent! >... - Page 14 5 Operation 2. Dispensing Hold the discharge tube and remove the closure cap/ screw cap. Hold the discharge tube orifice on the inner wall of a suitable receiving vessel. Push the piston down to the lower stop evenly and with minimal effort and then let it move up slowly.
- Page 15 Installing the flexible discharge tube Screw the tube holder to the valve block and install the collection tube. seripettor®: Before installing the flexible discharge tube, remove the built-in elastomer sealing ring and replace it with the provided PTFE sealing ring.
- Page 16 Luer-Lock. Never exceed the maximum operating force of 100 N. Assembly Dismantle the standard discharge tube from the seripettor® pro and slide the discharge tube with Luer- Lock approx. 2 mm onto the adapter for discharge tubes. Then firmly tighten the cap nut by hand. Commercially available sterile filters can be installed onto the Luer- Lock attachment.
- Page 17 5 Operation 5.4 Bottle stand For small bottles, and when using the flexible discharge tube, use a bottle stand to prevent tipping over (Accessories/spare parts). Assembly Position the mounting plate at the appropriate height. As shown, insert the instrument firmly into the holder until it audibly clicks into place.
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Page 18: Error Limits
6 Error limits 6 Error limits Error limits in relation to the nominal capacity (= maximum 20 °C volume) indicated on the instrument at equal temperature (20 ºC/68 °F) of instrument, ambient environment and dis- tilled water. Testing takes place according DIN EN ISO 8655- 6 with a completely filled instrument and with uniform and smooth dispensing. -
Page 19: Checking The Volume (Calibration)
This time frame should be adjusted to correspond with individual requirements. The com- plete testing procedure (SOP) can be downloaded at www.brand.de. For GLP- and ISO-compliant eval- uations and documentation, we recommend the EASYCAL™ calibration software from BRAND. Demo version can be downloaded from www.brand.de. -
Page 20: Basic Cleaning
8 Cleaning 8 Cleaning WARNING Components filled with reagent The cylinder, valves, telescopic filling tube and discharge tube contain reagent! > Never remove the discharge tube while the dispensing cylinder is filled. > Point the valves and tube openings away from your body. >... - Page 21 8 Cleaning 8.2 Cleaning and replacing valves 8.2.1 seripettor® Filling valve Remove filling tube. Unscrew the filling valve with a coin and extract the fill- ing valve body. If the sealing ring is contaminated or damaged, care- fully remove it using a pair of angled tweezers.
- Page 22 8 Cleaning Screw in the cleaned or new filling valve first by hand and then tighten it with a coin. Discharge valve Loosen the screw cap of the dispensing tube. Extract the discharge valve. If the sealing ring is contaminated or damaged, care- fully remove it using a pair of angled tweezers.
- Page 23 8 Cleaning 8.2.2 seripettor® pro Filling valve Remove filling tube. Use the assembly tool to unscrew the filling valve. If the sealing ring is contaminated or damaged, care- fully remove it using a pair of angled tweezers. Clean any contaminated parts (e.g., in an ultrasonic bath).
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Page 24: Replacing The Dispensing Cartridge
8 Cleaning Discharge valve The discharge valve is built into dispensing tube. Dismantle the dispensing tube and clean in an ultra- sonic bath if necessary. Install cleaned or new dispensing tube. 8.3 Dislodging a stick valve ball 8.4 Replacing the dispensing cartridge The dispensing cartridge is a wear part. - Page 25 8 Cleaning Release the piston lock. Unscrew and remove the actuating unit. Unscrew the dispensing cartridge and replace it with a new one, see Accessories/spare parts, p. 79. Screw the actuating unit on and engage the piston lock. 997468 Operating manual...
- Page 26 Allow to cool in the closed autoclave to avoid deformation due to too fast cooling (cooling time approx. 2 hours, excep- tion: seripettor® with agar culture medium up to max. 60 °C). After every autoclaving, inspect all parts for deformities or damage.
- Page 27 9 Autoclaving 9.1 Preparing for sterile work After autoclaving, seal the dispensing tube and tighten the screws. Under sterile conditions, remove the closure cap. Screw in the sterile dispensing cartridge (see Acces- sories/spare parts, p. 79). Fasten the actuating unit. 997468 Operating manual...
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Page 28: Troubleshooting
10 Troubleshooting 10 Troubleshooting Problem Possible cause Corrective action Piston sluggish Crystal deposits Replace dispensing cartridge, see Replacing the dis- pensing cartridge, p. 72). Forbidden liquid dispensed Clarify whether the medium is permitted to be dis- pensed, see Dispenser Selection Chart. Liquid is above the Dispensing cartridge leaky Replace dispensing cartridge, see Replacing the dis- dosing piston pensing cartridge, p. 72. -
Page 29: Product Markings
11 Product markings 11 Product markings Symbol or number Meaning General warning symbol Refer to the operating manual Use eye protection Use hand protection Wear protective clothing XXZXXXXX Serial number 997468 Operating manual... -
Page 30: Ordering Information
Gradu- Order ation ≤ ± % µl ≤ % µl 0.2–2 0.04 4720120 1 - 10 4720140 2.5–25 4720150 seripettor® pro Volume Gradu- Order ation ≤ ± % µl ≤ % µl 0.2–2 0.04 4720420 1 - 10 4720440 2.5–25... -
Page 31: Accessories/Spare Parts
13 Accessories/spare parts 13 Accessories/spare parts Bottle adapter Bottle adapter, PP, for seripettor® and seripettor® pro. Outer for bottle thread**/ Pkg unit Order no. thread for ground joint GL 32 GL 24-25 704325 GL 32 GL 28 / S* 28... - Page 32 Piston (PE), cylinder 704508 ile*** (PP). NOTICE The dispensing cartridges are not autoclavable. Discharge tube for seripettor® Discharge tube for seripettor® with closure cap, EPDM dis- charge valve and sealing ring. Model Pkg unit Order no. 2 ml, fine tip 704518...
- Page 33 Sealing rings for seripettor® Description Material Pkg unit Order no. Sealing ring EPDM 5 pc per set 6788 Flexible discharge tubing* for seripettor® and seripettor® pro Description Model Pkg unit Order no. PTFE, spiraled 2 + 10 ml 704522 tube, approx.
- Page 34 704534 additional 250, with 704536 sealing ring sealing ring 500, with 704538 sealing ring Telescoping filling tube for seripettor® pro Material Model Length Order no. (mm) unit 2 + 10 ml* 70 - 140 1 708210 125 - 240 1...
- Page 35 Material Model Order no. unit Stainless steel 2 ml 704541 lifting spring 10 ml 704542 25 ml 704544 Actuating unit for seripettor® pro Description Material Model Order no. unit Stainless steel PPO. PEI 2 ml 704551 lifting spring (UV protec-...
- Page 36 Model Pkg unit Order no. Valve block for 2 + 10 ml 6792 discharge tubing 25 ml 6794 for seripettor® and seripettor® Sealing cap for valve block Description Model Pkg unit Order no. Sealing cap for 2 + 10 ml...
- Page 37 Number. Place the Return Authorization number so that it is clearly visible on the outside of the package. Contact addresses Germany: USA and Canada: BRAND GMBH + CO KG BrandTech® Scientific, Inc. Otto-Schott-Straße 25 11 Bokum Road 97877 Wertheim (Germany)
- Page 38 14 Repairs info@brand.co.in www.brand.cn.com www.brand.co.in Operating manual 997468...
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Page 39: Calibration Service
For customers outside Germany If you would like to use our calibration service, please contact one of our service partners in your re- gion. If a factory calibration is requested, the service partner can forward the instrument to BRAND. 997468... -
Page 40: Warranty
16 Warranty 16 Warranty We shall not be liable for the consequences of improper handling, use, servicing, operating or unau- thorized repairs of the device or for the consequences of normal wear and tear, especially of wearing parts such as pistons, seals, valves and the breakage of glass. The same applies for failure to follow the instructions of the operating manual. - Page 41 17 Disposal 17 Disposal Before disposal, observe the relevant national disposal regulations, and ensure that the product is dis- posed of properly. 997468 Operating manual...
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