BD FACSDiscover S8 Quick Start Manual

Job Aid
BD FACSDiscover S8 Cell Sorter:
Performing a daily system shutdown
This job aid contains instructions for how to perform a daily shutdown. A daily shutdown cleans the sample path and fills the flow cell
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with BD Detergent Solution in preparation for shutdown.
NOTE If you are shutting down the system for more than two days, select the long-term shutdown procedure. See the job aid for
performing a long-term shutdown.
For additional information, see the Maintenance section in the BD FACSDiscover S8 Cell Sorter with BD CellView Image
Technology and BD SpectralFX Technology User's Guide.
S t a r t u p S e t u p
s y s t e m e x p e r i m e n t
Materials Needed
• 10% bleach (approximately 3 mL)
• Deionized (DI) water
• BD
®
Detergent Solution Concentrate
Before you begin
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Prepare 3 mL of 1.5% BD Detergent Solution from the BD
the instructions on the label for correct dilution information and see instead the safety data sheet regarding handling.
®
CAUTION Never mix BD Detergent Solution and bleach because it can create dangerous fumes.
Cleaning the sample line
This procedure cleans the sample line with a bleach solution. We recommend cleaning the sample line at the end of each experiment,
between users, and before shutting down.
Load a tube containing 3 mL of a 10% bleach solution onto the sample loading port.
1.
From an open experiment, from the View Data tab, click Load.
2.
3. After approximately 5 minutes, click Unload.
Load a tube containing 3 mL of DI water onto the sample loading port.
4.
NOTE It is important to always run a tube of DI water after running bleach on the cell sorter.
Repeat steps 2 and 3.
5.
E s t a b l i s h S o r t a n d
s e t t i n g s a n a l y z e
®
Detergent Solution Concentrate. Do not dilute the entire bottle. Follow
S h u t d o w n
M a n a g e d a t a
s y s t e m
1