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BD FACSDiscover S8 Quick Start Manual

Cell sorter: performing a daily system shutdown

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Job Aid
BD FACSDiscover S8 Cell Sorter:
Performing a daily system shutdown
This job aid contains instructions for how to perform a daily shutdown. A daily shutdown cleans the sample path and fills the flow cell
®
with BD
Detergent Solution in preparation for shutdown.
NOTE If you are shutting down the system for more than two days, select the long-term shutdown procedure. See the job aid for
performing a long-term shutdown.
For additional information, see the Maintenance section in the BD FACSDiscover S8 Cell Sorter with BD CellView Image
Technology and BD SpectralFX Technology User's Guide.
S t a r t u p
S e t u p
s y s t e m
e x p e r i m e n t
Materials Needed
• 10% bleach (approximately 3 mL)
• Deionized (DI) water
• BD
®
Detergent Solution Concentrate
Before you begin
®
Prepare 3 mL of 1.5% BD
Detergent Solution from the BD
the instructions on the label for correct dilution information and see instead the safety data sheet regarding handling.
®
CAUTION Never mix BD
Detergent Solution and bleach because it can create dangerous fumes.
Cleaning the sample line
This procedure cleans the sample line with a bleach solution. We recommend cleaning the sample line at the end of each experiment,
between users, and before shutting down.
Load a tube containing 3 mL of a 10% bleach solution onto the sample loading port.
1.
From an open experiment, from the View Data tab, click Load.
2.
3.
After approximately 5 minutes, click Unload.
Load a tube containing 3 mL of DI water onto the sample loading port.
4.
NOTE It is important to always run a tube of DI water after running bleach on the cell sorter.
Repeat steps 2 and 3.
5.
E s t a b l i s h
S o r t a n d
s e t t i n g s
a n a l y z e
®
Detergent Solution Concentrate. Do not dilute the entire bottle. Follow
S h u t d o w n
M a n a g e d a t a
s y s t e m
1

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Summary of Contents for BD FACSDiscover S8

  • Page 1 NOTE If you are shutting down the system for more than two days, select the long-term shutdown procedure. See the job aid for performing a long-term shutdown. For additional information, see the Maintenance section in the BD FACSDiscover S8 Cell Sorter with BD CellView Image Technology and BD SpectralFX Technology User’s Guide.
  • Page 2 For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD Life Sciences, Milpitas, California, 95035, USA bdbiosciences.com BD, the BD Logo, BD CellView, BD FACSChorus, BD FACSDiscover and BD SpectralFX are trademarks of Becton, Dickinson and Company or its affiliates. ©2024 BD. All rights reserved. NPM-1218 (v2.0)