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Related Manuals for Bibby Sterilin Techne TC-5000
Summary of Contents for Bibby Sterilin Techne TC-5000
- Page 1 TC-5000 OPERATOR'S MANUAL Issue 1 5/09...
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Page 2: Table Of Contents
CONTENTS PAGE INTRODUCTION SAFETY AND INSTALLATION INFORMATION Operator Safety Warning Unpacking Installation Replacement Cable Working Conditions Guarantee SÉCURITÉ ET CONSIGNES D'INSTALLATION Sécurité de l'opérateur Avertissement Déballage Installation Câble de rechange Conditions de travail Garantie SICHERHEITS - UND INSTALLATIONSINFORMATIONEN Sicherheit des Bedienpersonals Achtung Auspacken Inbetriebnahme... - Page 3 INSTRUMENT CONNECTIONS CONTACT INFORMATION TC-5000 SPECIFICATION TC-5000 OPERATION AND MAINTENANCE Gradient Adjustable Heated Lid Adjustable Screen Consumables PC Connections Cleaning the TC-5000 Removing the Block PROGRAMMING THE TC-5000 Start up Menu The System User Menu Writing and Editing Programs Option 1: Copying an Existing Template Option 2: Editing and Saving an Existing Template Option 3: Creating a New Program Inserting a Segment...
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Page 4: Introduction
INTRODUCTION The TC-5000 is a gradient thermal cycler, designed to accurately control the temperatures of samples between 4°C and 99°C. It has many scientific applications including DNA amplification, PCR , reverse transcription and sequencing. The TC-5000 is programmed via a touch screen LCD graphics display. The user is able to input various programs and parameters, including: •... -
Page 5: Safety And Installation Information
SAFETY AND INSTALLATION OPERATOR SAFETY Please read this manual carefully before using the thermal cycler. If the equipment is not used in the manner described in this manual the protection provided by the equipment might be impaired. All Bibby Scientific Ltd instruments are designed to conform to international safety requirements and are fitted with an over-temperature cut-out. -
Page 6: Replacement Cable
rating plate (located on the back of the unit). Ensure that the voltage selector switch (located above the On/Off switch) is set to the appropriate voltage for the local supply. Please note that the unit must be earthed to ensure proper electrical safety. Connect the mains cable to a suitable plug according to the table below. -
Page 7: Guarantee
GUARANTEE The instrument is guaranteed against any defect in material or workmanship for the period specified on the enclosed guarantee card. This period is effective from the date of purchase; within this period all defective parts will be replaced free of charge provided that the defect is not the result of an accident, misuse or negligence. -
Page 8: Sécurité Et Consignes D'installation
SÉCURITÉ ET CONSIGNES D'INSTALLATION SÉCURITÉ DE L’OPÉRATEUR Lire attentivement le présent manuel avant d'utiliser le cycleur thermique. Si ce matériel n'est pas utilisé de la manière décrite dans le présent manuel, la protection fournie risque d'être compromise. Tous les appareils Bibby Scientific Ltd sont conçus pour être conformes aux exigences internationales de sécurité... -
Page 9: Câble De Rechange
2. Avant de raccorder l'appareil à l'alimentation électrique sur secteur, vérifier la tension par rapport à la plaque signalétique (située au dos de l'appareil). Vérifier que le sélecteur de tension (situé au- dessus de l'interrupteur Marche/Arrêt) est réglé sur la tension correspondant au réseau local. Noter que l'appareil doit être relié... -
Page 10: Garantie
L'appareil a été testé en matière de perturbations radioélectriques et est certifié selon la norme EN61326. GARANTIE L'appareil est garanti contre tout défaut de matériaux ou vice de fabrication pendant la période précisée sur la carte de garantie jointe. Cette période s'applique à compter de la date d'achat; pendant cette période, toutes les pièces défectueuses seront remplacées gratuitement à... -
Page 11: Sicherheits - Und Installationsinformationen
SICHERHEITS - UND INSTALLATIONSINFORMATIONEN SICHERHEIT DES BEDIENPERSONALS Lesen Sie diese Anleitung vor Verwendung des Thermocyclers bitte sorgfältig durch. Wenn das Gerät nicht entsprechend der Bedienungsanleitung eingesetzt wird, können die Schutzfunktionen des Gerätes beeinträchtigt werden. Alle Geräte von Bibby Scientific Ltd entsprechen den internationalen Sicherheitsanforderungen und sind mit einer Übertemperatur-Schutzvorrichtung ausgestattet. -
Page 12: Ersatzkabel
1. Alle Geräte von Bibby Scientific Ltd werden mit einem Netzkabel geliefert, das entweder eingesteckt wird oder fest mit dem Gerät verbunden ist. 2. Vor dem Anschluss bitte kontrollieren, ob die Stromversorgung den Angaben auf dem Typenschild (auf der Geräterückseite) entspricht. Darauf achten, dass der Spannungswähler (über dem Ein/Aus- Schalter) auf die entsprechende örtliche Spannung eingestellt ist. -
Page 13: Garantie
Hinweis: Die Gerätespezifikationen beziehen sich auf eine Umgebungstemperatur von 20°C und können sich außerhalb des Bereichs 10°C bis 30°C verschlechtern. Das Gerät wurde auf HF-Störeinflüsse geprüft und entspricht den EMV-Bedingungen nach EN61326. GARANTIE Bibby Scientific Ltd gewährleistet, dass dieses Gerät für den auf der Garantiekarte angegebenen Zeitraum keine Herstellungs- und Materialmängel aufweist. -
Page 14: Información De Seguridad E Instalación
INFORMACIÓN DE SEGURIDAD E INSTALACIÓN SEGURIDAD DEL OPERARIO Lea atentamente este manual antes de utilizar el ciclador térmico. Si el equipo no se utiliza de la forma descrita en este manual, se reducirá la protección ofrecida por el equipo. Todos los instrumentos Bibby Scientific Ltd están diseñados para cumplir los requisitos internacionales de seguridad, e incluyen un dispositivo de corte de sobretemperatura. -
Page 15: Cable De Repuesto
2. Antes de conectar el instrumento al suministro eléctrico, compruebe que el voltaje coincida con el indicado en la placa de régimen (situada en la parte trasera de la unidad). Asegúrese de que el interruptor de selección de voltaje (situado encima del interruptor de encendido/apagado) está ajustado al voltaje correcto del suministro eléctrico. -
Page 16: Garantía
especificaciones pueden empeorar si se utiliza el instrumento fuera del intervalo de temperatura comprendido entre 10°C y 30°C. Se han realizado pruebas para comprobar la interferencia de radiofrecuencia del instrumento, el cual cumple la normativa EN61326. GARANTÍA El instrumento está garantizado contra cualquier defecto en el material o la fabricación durante el período especificado en la tarjeta de garantía que se adjunta. -
Page 17: Informazioni Sulla Sicurezza E L'installazione
INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONE SICUREZZA DELL'OPERATORE Leggere attentamente il presente manuale prima di usare il ciclatore termico. Se non si usa l'apparecchiatura nel modo descritto nel presente manuale, la protezione fornita dall'unità potrebbe risultarne diminuita. Tutti gli strumenti Bibby Scientific Ltd sono progettati per rispettare i requisiti di sicurezza internazionali e sono montati con un sezionatore di sovratemperatura. -
Page 18: Cavo Di Ricambio
2. Prima di collegare lo strumento all'alimentazione elettrica di rete, controllare la tensione confrontandola con la targhetta riportante i valori nominali (si trova sul retro dell'unità). Assicurarsi che il selettore di tensione (posto sopra l'interruttore On/Off) sia impostato sulla tensione corretta per l'alimentazione locale. -
Page 19: Garanzia
Nota: le specifiche di controllo sono indicate ad una temperatura ambiente di 20°C. Le specifiche potrebbero peggiorare fuori da una temperatura ambiente compresa tra 10°C e 30°C. Lo strumento è stato collaudato per interferenze da radiofrequenze ed è certificato secondo la norma EN61326. -
Page 20: Instrument Connections
INSTRUMENT CONNECTIONS Serial Port RS232/RS485 Selector: Select RS485 for multiple units or RS232 for a single Parallel Port unit RS 485 Voltage Selector: RS 485 Select 230V or 115V RS 232 RS 485 RS485 Connections and ensure correct fuses are fitted TERMINATION D AISY CHAIN... -
Page 21: Contact Information
CONTACT INFORMATION For technical, sales or servicing information, contact your local Bibby Scientific Ltd dealer or: UK and Rest of World North and South America Bibby Scientific Ltd Techne Inc. Beacon Road, Stone, 3 Terri Lane, Suite 10, Staffordshire, ST15 0SA, UK Burlington, NJ, 08016, USA Tel: +44 (0)1785 812121 Toll free: 800-225-9243... -
Page 22: Tc-5000 Specification
TC-5000 SPECIFICATION Technical Specification at an ambient temperature of 20°C Temperature Gradient Range 20°C to 70°C Maximum Gradient 30°C Minimum Gradient 1°C Number of temperature probes Block temperature range 4°C to 99°C Typical heating rate 3.0°C/sec Typical cooling rate 1.3°C/sec <... - Page 23 Miscellaneous Height x Width x Length 260mm x 220mm x 420mm Weight 12.5kg Power consumption 620W (including the heated lid) Parallel port Serial port RS232/RS485 adaptable Connection to PC...
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Page 24: Tc-5000 Operation And Maintenance
TC-5000 OPERATION AND MAINTENANCE The TC-5000 has many features which increase the flexibility of the instrument and improve the ease of use. The following features are described in more detail in this chapter: • Gradient • Adjustable heated lid • Adjustable screen •... -
Page 25: Adjustable Heated Lid
It is important to remember that the greater the gradient, the poorer the uniformity across the block, therefore fine-tuning may be required to establish the optimum temperature. While the gradient is mainly linear there are small variations at the extremes of the gradient due to a loss of heat from the hottest part of the block and a gain of heat from the adjacent column on the coldest. -
Page 26: Adjustable Screen
A heated lid mat (Part No. 6104978) is supplied with the TC-5000; this is for use with 96-well PCR plates sealed with an adhesive seal. The rubber mat should be placed in top of the sealing film to ensure good thermal contact with the heated lid. Do not over-tighten the lid as this will cause the silicone rubber membrane to deform and will reduce the effectiveness of the heated lid. -
Page 27: Pc Connections
PC CONNECTIONS It is possible to connect either a single thermal cycler or a series of instruments to a computer. The options are: 1. To connect a single thermal cycler to a computer part number FGEN232 is required which contains a connecting cable, software disk and instructions. -
Page 28: Cleaning The Tc-5000
CLEANING THE TC-5000 Prior to cleaning the thermal cycler it is important to disconnect the unit from the power supply and if it has been in use allow both the block and heated lid to cool to below 50°C prior to attempting to clean it. -
Page 29: Removing The Block
REMOVING THE BLOCK The thermal cycling block can be removed for detailed cleaning or replacement. First ensure that the unit is disconnected from the power supply and has been allowed to cool if the unit was previously in use. The two quick release handles located at the front and back of the block are used to unlock the block. -
Page 30: Programming The Tc-5000
PROGRAMMING THE TC-5000 START UP MENU When the TC-5000 is first switched on the screen shown below is displayed and will remain until the unit has finished performing a series of self tests. This screen displays information such as the serial number of the instrument, the type of block being used and the version of software running the thermal cycler. -
Page 31: The System User Menu
THE SYSTEM USER MENU In this section of the software the user is able to write new programs and edit existing ones. Additionally the program defaults for parameters such as the heated lid and final extension steps can be altered. The gradient calculator and data log files can also be accessed through their sub-menus. To access the programming software press the "System User"... -
Page 32: Writing And Editing Programs
WRITING AND EDITING PROGRAMS There are three options available for creating programs on the TC-5000: 1. Copy and edit one of the pre-written templates. 2. Edit a pre-written template and save it using a different name. 3. Create a new program with user-specific parameters. There are 5 pre-written templates. -
Page 33: Option 1: Copying An Existing Template
OPTION 1: COPYING AN EXISTING TEMPLATE Techne TC-5000 To access the program templates select "Programs" in the System Users menu. Programs Program Gradient Exit Defaults Calculator The cursor arrows can be used to scroll up Select Program and down the stored programs. An individual 14526 bytes of memory free program is opened by pressing on the b 2 STEP PCR TEMPLATE... - Page 34 Select the copied program. Select Program 14526 bytes of memory free b ICE BUCKET b LITIGATION 15 DEGREES b RT PCR TEMPLATE i B ACTIN i GAPDH ▼ Exit The program cannot be changed at this point Program so press "Edit" to make any changes B ACTIN required.
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Page 35: Option 2: Editing And Saving An Existing Template
OPTION 2: EDITING AND SAVING AN Select Program EXISTING TEMPLATE 14526 bytes of memory free Select the template from the program menu. b 2 STEP PCR TEMPLATE b 3 STEP PCR TEMPLATE b ICE BUCKET b LITIGATION 15 DEGREES b RT PCR TEMPLATE ▼... -
Page 36: Option 3: Creating A New Program
OPTION 3: CREATING A NEW PROGRAM Select Program The final option is to create a completely new 14526 bytes of memory free program by selecting "New" in the Select b 2 STEP PCR TEMPLATE Program menu. This section also contains b 3 STEP PCR TEMPLATE details of the individual parameters and b ICE BUCKET... -
Page 37: Inserting A Segment
The next step enables one of the following to Edit program function be added to the program: Next step Segment: Enables the temperature, time and heating rate to be inserted into the segment. ➔ ➔ Segment Additionally, a gradient across the block can be added. -
Page 38: Inserting Cycle Numbers
The time can be set in hours (h), minutes (m) or Edit program function seconds (s). Type in the time required followed ➔ by the unit. Use the to move across the Segment 94.0°C 0°C 00m30s MAX 0°C/s screen to the ramp rate. ➔... - Page 39 Cycle 1 Cycle 2 Cycle 3 Cycle 4 Decremental temperature for Touchdown DNA amplification Time The Hold Temperature of the incremented/decremented step is the defined Hold Temperature plus the summation of the increments/decrements. The first cycle is never altered, only subsequent cycles. If decrement is selected, the Hold Temperature is prevented from falling below 4°C.
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Page 40: Inserting A Pause
INSERTING A PAUSE Selecting "Pause" adds a stop to the program, which when running requires user Edit program function intervention before the program can PAUSE continue. ➔ ➔ Segment Number Continue Cycles Cancel Pause • SAVING CHANGES TO A PROGRAM To change a saved program, select the Editing program and when opened select "Edit"... - Page 41 Select the program required, using the cursor Select Program keys to view all the available programs. 14526 bytes of memory free b 2 STEP PCR TEMPLATE b 3 STEP PCR TEMPLATE b ICE BUCKET b LITIGATION 15 DEGREES b RT PCR TEMPLATE ▼...
- Page 42 As the program continues the temperatures B ACTIN Program are displayed on the screen, as shown Cycle 3 of 3. Step 2 of 3 Holding at 55.0°C 10°C opposite. Sample Lw= 50.0°C, Sample Up= 60°C Time to complete 01m26s 00h01m 00h02m 00h03m 00h04m...
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Page 43: Program Defaults
This screen is displayed if there is a power loss B ACTIN Program during the running of the program and the Auto Restart Off 15/05/2006 10:21:28 auto-restart function is switched on. The On 15/05/2006 10:21:36 display shows the times at which the power failed and resumed. - Page 44 Pause before program: This allows the insertion of a pause in the experiment before the experiment starts. After the pause there must be user intervention before the program can move on to the next step of the program. Initial denaturation: Many experimental Edit initial denaturation setting protocols require a high temperature incubation to remove any secondary structure...
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Page 45: Gradient Calculator
GRADIENT CALCULATOR The gradient is set within the program (see page 36 for further details), however, it is not possible to view the actual temperatures of each column in the program. The gradient calculator calculates the temperatures for each of the 12 columns (24 for the 384 well block) and allows the user to determine the optimal amplification conditions for the assay. -
Page 46: System Administration
SYSTEM ADMINISTRATION This section of the software contains settings for the instrument, rather than the actual programs. From various sub-menus it is possible to change passwords for programs and the instrument, test the instrument, delete current programs stored on the memory card and completely reset the instrument. To access the System Admin simply press the "System Admin"... -
Page 47: Program Passwords
The following functions are located within the System Administration menu: Program Passwords: Enables an administrator to change the password for any program. Sys Admin Password: Allows the administrator to change the administrator password. System Test: Initiates a simple diagnostic test. Controller Reset: Allows the user to restore the factory settings. -
Page 48: System Test
SYSTEM TEST This is a diagnostic test to ensure that the Techne TC-5000 Real Time Clock settings are correct. RTC OK Exit CONTROLLER RESET If "Confirm Reset" is pressed the instrument Techne TC-5000 default settings will be restored. WARNING! All files and programs which *** Controller Reset *** WARNING!! have been stored on the internal memory... -
Page 49: System Configuration
SYSTEM CONFIGURATION This contains all the settings for programming the instrument such as units of temperature, date format, language, and date and time settings. Other important features are enabling the auto restart in case of a power failure and setting the comms address of the instrument if multiple units are linked to a computer. - Page 50 Date: The date can be corrected by the user by using the numerical interface shown above. Time: The time can be corrected by the user by using the numerical interface shown in above. Auto restart: This allows a program to be re-started in the event of a power failure when the instrument is in the middle of running a program.
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Page 51: Frequently Asked Questions
FREQUENTLY ASKED QUESTIONS What is "Hot start"? The Hot Start step is used to pause the machine at a specific temperature, typically around 70°C, after the initial template denaturation. The reason is to allow the manual addition of unmodified Taq DNA polymerase which may loose activity if added during the initial 5min denaturation. Heat-activated Taq or Hot Start enzymes do not require this step. -
Page 52: Additional Information
ADDITIONAL INFORMATION Brief fault finding notes and a list of replacement parts are given in this section. Note: that this equipment should only be dismantled by properly trained personnel. Removing the outer cover exposes potentially lethal mains voltages. There are no user serviceable parts within this equipment. -
Page 53: Pc Accessories
Product Number Description Quantity FHSEALSD Heat sealer FHSPA96 Heat sealer plate adapter for 96-well plates FHSPA384 Heat sealer plate adapter for 384-well plates FTC51BHD TC-5000 block for 96 x 0.2ml microtubes or 96-well plate FTC51BFD TC-5000 block for 96 x 0.2ml microtubes or 96-well fully skirted plate FTC51B5D TC-5000 block 60 x 0.5ml microtubes... - Page 54 Bibby Scientific Italia Srl Bibby Scientific France SAS ZI du Rocher Vert - BP 79, Via Alcide de Gasperi 56, 77793 Nemours Cedex, 20077 Riozzo di Cerro al Lambro, Milano, Italia France Tel: +33 1 64 45 13 13 Tel: +39 (0)2 98230679 Fax: +33 1 64 45 13 00 Fax: +39 (0)2 98230211 e-mail: marketing@bibby-scientific.it...
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