Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Subscribe to Our Youtube Channel
Related Manuals for Bibby Sterilin Techne Prime Series
Summary of Contents for Bibby Sterilin Techne Prime Series
- Page 1 Prime, PrimeX and PrimeG oPerator's manual Issue 1.1 03/12...
-
Page 3: Table Of Contents
contents PaGe IntroDuctIon BeFore use Unpacking saFetY InFormatIon English Français Deutsch Italiano Español contact InFormatIon PrIme sPecIFIcatIon InstallatIon General soFtWare Features Introduction Template programs Incremented/decremented time and temperature Gradient ( PrimeG only) Oligonucleotide T calculator ProGrammInG teXt, temPerature anD tIme Text entry Temperature entry Time entry... - Page 4 PaGe run a ProGram Edit Copy Delete Search uPDatInG tHe PrIme soFtWare uPGraDInG From PrIme to PrImeG FaQs tecHnIcal suPPort anD serVIcInG aDDItIonal InFormatIon User Maintenance Fault Finding Fuses Insulation Testing accessorIes rePlacement Parts...
-
Page 5: Introduction
IntroDuctIon Prime range is a family of personal thermal cyclers designed to accurately control the temperatures of samples between 4ºC and 100°C. They have many scientific applications including PCR , reverse transcription, ligation and sequencing. The basic Prime unit can accommodate 24 x 0.2ml tubes in a flexible 8 x 3 array or 18 x 0.5ml tubes. -
Page 6: Before Use
BeFore use Before using the Prime please ensure you have read this manual carefully. If there is any doubt relating to the proper use of this equipment, the staff at Bibby Scientific Ltd. or your supplier will be happy to assist you. unPackInG When unpacking the unit please ensure that the following have been removed from the packaging: Prime with block... -
Page 7: Safety Information
saFetY InFormatIon Please read all the information in this manual before using the Prime. WarnInG HIGH TEMPERATURES ARE DANGEROUS: they can cause serious burns to operators and ignite combustible material. Users should be aware of the following potential hazards: USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS ●... - Page 8 enVIronmental conDItIons Prime is designed operate under the following conditions: Indoor use ● Ambient temperature range +5ºC to +40ºC ● Altitude to 2000m ● Relative humidity not exceeding 80% ● Mains supply fluctuations not exceeding 10% ● Over voltage category II IEC 60364-4-443 ●...
-
Page 9: Français
l’InFormatIon De sÛretÉ Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le Prime. aVertIssement Les TEMPÉRATURES ÉLEVÉES SONT DANGEREUSES car elles peuvent provoquer de graves brûlures chez l’opérateur et enflammer les matériaux combustibles. Les utilisateurs doivent porter une attention toute particulière aux points suivants : PROCEDER AVEC PRUDENCE ET PORTER DES GANTS POUR SE PROTEGER LES MAINS ●... - Page 10 conDItIons enVIronnementales Prime est conçu pour fonctionner dans les conditions suivantes: Pour un usage intérieur seulement ● Température ambiante +5°C à +40°C ● Altitude inférieure à 2000m ● Humidité relative ne dépassant pas 80% ● Fluctuations de l’alimentation n’excédant pas 10% de la valeur nominale ●...
-
Page 11: Deutsch
sIcHerHeIts- InFormatIonen Lesen Sie diese Anleitung vor Verwendung des Prime bitte sorgfältig durch. acHtunG HOHE TEMPERATUREN STELLEN EINE GEFAHRENQUELLE DAR. Sie können schwere Brandverletzung verursachen und brennbare Stoffe entzünden. Der Benutzer sollt sich mit den möglichen Gefahren vertraut machen: UMSICHTIG VORGEHEN UND SCHUTZHANDSCHUHE TRAGEN ●... - Page 12 umWeltBeDInGunGen Prime ist für den Einsatz unter folgenden Bedingungen ausgelegt: Gebrauch in Innenräumen ● Umgebungstemperatur zwischen +5ºC to +40ºC ● Höhe: bis zu 2000 m ● Relative Feuchte nicht über 80% ● Netzspannungsschwankungen nicht über 10% ● Überspannungsklasse 2 IEC 60364-4-443 ●...
-
Page 13: Italiano
InFormaZIonI DI sIcureZZa Leggere attentamente il presente manuale prima di usare il Prime. aVVertenZa Le ALTE TEMPERATURE SONO PERICOLOSE in quanto possono provocare serie ustioni agli operatori e dare fuoco al materiale combustibile. Gli utenti devono conoscere i seguenti pericoli potenziali: PRESTARE ATTENZIONE ED INDOSSARE GUANTI PROTETTIVI PER LE MANI ●... - Page 14 conDIZIonI amBIentalI Prime è stato progettato per funzionare nelle seguenti condizioni: uso interno ● range di temperatura ambiente da +5ºC a +40ºC ● altitudine massima 2000 m. ● umidità relativa non superiore all’80% ● oscillazione dell’alimentazione di rete non superiore al 10% ●...
-
Page 15: Español
InFormacIón De seGurIDaD Lea atentamente este manual antes de utilizar el Prime. aDVertencIa LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que pueden ocasionar quemaduras graves a los operarios y prender el material combustible. Los usuarios deben conocer los posibles riesgos: TENGA CUIDADO Y LLEVE GUANTES DE PROTECCIÓN PARA PROTEGERSE LAS MANOS ●... - Page 16 conDIcIones amBIentales Prime está diseñado para utilizarse en las condiciones siguientes: Uso en interior ● Intervalo de temperatura ambiente +5ºC a +40ºC ● Altitud: hasta 2000 m ● Humedad relativa no superior al 80% ● Fluctuaciones del suministro eléctrico no superiores al 10% ●...
-
Page 17: Contact Information
contact InFormatIon For technical, sales or servicing information, contact your local Techne dealer or: north and south america Bibby Scientific Ltd. Bibby Scientific US Inc. t/a Techne Inc. 3 Terri Lane, Suite 10 Beacon Road Stone Burlington, N.J. 08016 Staffordshire ST15 0SA Toll Free (in NA): 800-225-9243 Tel: +1 609 589 2560... -
Page 18: Prime Specification
PrIme sPecIFIcatIon Prime PrimeX PrimeG Sample capacity: 0.2ml Sample capacity: 0.5ml Block temperature range 4ºC to 100ºC Block uniformity (at 50ºC) <±0.3ºC Temperature accuracy (at 50ºC) <±0.2ºC Maximum heating rate 3.0ºC/s Maximum cooling rate 2.1ºC/s Gradient Can be enabled Gradient availability 30ºC to 80ºC Maximum gradient range 14ºC... -
Page 19: Installation
InstallatIon see also the safety Information sections on pages 5 to 14. Front view rear view Lid adjustment knob ( PrimeX and G only). 1. USB port for service and calibration only. Lid release latch. 2. On/Off rocker switch. 3.5” colour VGA LCD touch screen display. 3. - Page 20 6. With the PrimeX and G, the heated lid has a rotating knob to adjust the lid pressure on the samples, allowing for a variety of consumables to be used. To adjust the pressure: a. Place the samples in the block and place the lid in the down position.
-
Page 21: General Software Features
General soFtWare Features IntroDuctIon Prime software allows the user to quickly and easily create thermal cycling programs using a simple and clear touch screen format. The software is structured into three modules which are accessed from the Home Screen. These allow the user to: Run an existing program, selecting from templates or ●... -
Page 22: Incremented/Decremented Time And Temperature
IncrementeD/DecrementeD tIme anD temPerature The software allows the user to either increment or decrement the time and/or temperature of a step within a cycling stage. These features are used with applications such as touchdown PCR where the annealing temperature is gradually decreased during the cycling process and long PCR, where due to the size of the product, long annealing/extension times are required and the extension time is increased by 15 to 20s per cycle during the final stages of the reaction. -
Page 23: Oligonucleotide T Calculator
olIGonucleotIDe t calculator One of the most important parameters in a PCR is the annealing temperature of the reaction. This can be determined theoretically using various software programmes to calculate the melting temperature (T ) of the primers. The T is defined as the temperature at which half the DNA strands are single stranded and half are double stranded when base paired to a complimentary strand. -
Page 24: Programming Text, Temperature And Time
ProGrammInG teXt, temPerature anD tIme This section gives instructions for the basic programming of text, temperature and time which are required in later sections. teXt entrY When giving a name to a program or stage a text entry screen will be opened. Touch a character/numerical button repeatedly to ●... - Page 25 simple temperature entry If necessary, first touch the button next to the parameter ● (e.g. Heated Lid) to turn it on. Next touch the temp button. ● A screen similar to that shown opposite is opened. The maximum and minimum allowed values are indicated depending on the parameter being set.
- Page 26 advanced temperature entry First ensure the number of cycles for the stage has been set ● then enter the required hold temperature as described above. note: Increments and Decrements will be inactive unless the number of cycles set is greater than 1. to increase or decrease the step temperature per cycle: Touch the step temp button.
- Page 27 The temperature value will now appear orange in the programming screen indicating that it contains a modified function. note: It is not possible to both increase and decrease the temperature of the same step. to enter a temperature gradient on a step PrimeG only): PrimeG an additional temperature Gradient button On the...
-
Page 28: Time Entry
The temperature value will now appear orange in the programming screen indicating that it contains a modified function. note: It is not possible to have both a gradient and an increment or decrement temperature in the same step. tIme entrY When touching a time parameter a time entry screen will open. - Page 29 Touch the white time area to open the time entry screen. ● Enter the time using the up and down arrow keys ● (h, m, s). Once the time is correct, touch ok to return to the ● previous screen. This screen will now display the set time.
- Page 30 A new screen will open which is similar to the simple time entry screen. Enter the required value (h, m, s) followed by ok. ● If an entered time value is invalid, a prompt will indicate ● the acceptable range. Touch accept to return to the previous screen.
-
Page 31: Instrument Settings And Defaults
Instrument settInGs anD DeFaults Touch the settings button to access the instrument Settings menu. Touch any of the buttons to view and edit the settings. ● Touch more settings to access further options. ● Once all the settings and defaults have been set as ●... - Page 32 If Auto Resume is set to always and the power is interrupted, on return of the power supply, a message will be displayed on the screen giving details of when the power was lost, when it was restored and the name of the program running at the time.
- Page 33 administrator settings This allows an administrator to remove passwords from password-protected programs, change the Administrator password and restore the unit to its factory settings. Touch none and enter the administrator password ● followed by Back. The default password is 1234. Touch ok to enter the Administrator Settings menu.
-
Page 34: Program Defaults
restore Factory settings All changes to the instrument defaults and settings can be removed and the unit returned to its factory settings. From the Administrator menu touch restore Factory ● settings. Touch the none button and enter the administrator ● password followed by Back. - Page 35 Pause This will cause the unit to pause before the program starts at the ambient temperature of the block (unless Sample Cooling has been set to on when the unit will pause with the block held at 4ºC). The program will not commence until the unit is manually un-paused.
-
Page 36: Service Settings
serVIce settInGs The service settings are for the use of authorised service engineers only. A service engineer’s password is required to access these settings. GraDIent calculator ( G onlY) rime The Gradient Calculator can be used to calculate the temperature for each column of the block for a user-specified temperature and gradient range. - Page 37 Touch the a c G t buttons to enter the primer sequence. ● Touch the arrow to delete the last character. ● Use the < and > keys to scroll through the sequence. ● Touch Back to return to the previous screen ●...
-
Page 38: Create A New Program
create a neW ProGram From the Home Screen, touch the new Program button. This will open a screen where you can build a program by adding stages and steps and define the temperatures and hold times. Insert/Delete a staGe A stage is a section of a program that consists of a number of individual steps that can be cycled (repeated) a defined number of times. -
Page 39: Insert/Delete A Step
ramp rate Touch the ramp rate setting to adjust the ramp rate. ● The range is 0.1ºC/s (minimum) to 3.0ºC/s maximum for heating and 2.1ºC/s for cooling. The value can be entered to 1 decimal place. Ramp rate will affect the heating or cooling rate from the previous step to the current step. -
Page 40: The More Button
tHe more Button The more button can be used to access the Program options and Instrument settings defaults for editing in the current program. Program options Touch this to view the program defaults as set up in the ● Settings module. Use this also to name the program and add a password to protect it from editing by other users. - Page 41 Initial denaturation, Final extension and Final Hold Touch the time and temperature buttons to edit these if ● required. Use the Down arrow to scroll to further options. ● Pause and Hot start Touch on/off to toggle between settings. ● Set the temperature for Hot Start if required.
-
Page 42: Settings
settInGs Touch the settings button to view the instrument ● defaults as set up in the instrument Settings module. Any of these parameters can be edited by touching the appropriate button and will be saved for the current program only. Touch Back twice to return to the programming screen. -
Page 43: Run A Program
run a ProGram From the Home Screen, touch the run button. This will open a screen where you can select a program to run, edit, copy or delete. Touch a program to select it. Use the arrow buttons to ● scroll down the list of stored programs. -
Page 44: Run
Touching the Run button sends the selected program to the Prime, ready to start the run. the Pre-run screen This screen shows a step-by step representation of the program in a tile format. The program name is given at the top of the screen followed by the stage name. -
Page 45: Edit
Touch temperature log to view the block temperatures. ● The previous two minutes of temperature readings can be viewed by scrolling across the screen using a finger. note: Touch Program View to return to the program status. eDIt editing existing programs First select the required program and touch edit. -
Page 46: Copy
editing password protected programs If the edited program is password protected, touching save will open the message box shown opposite. To save the changes either: Touch the none button next to Password and enter the ● correct password followed by save. note: this will overwrite the existing program. -
Page 47: Delete
to copy a program file to or from a usB memory stick: Insert a USB memory stick into the port on the front of ● the unit. A message screen will appear confirming that the USB memory stick has been inserted. Touch Yes to view the files on the USB memory stick or ●... -
Page 48: Search
searcH Programs saved on the unit can be searched by program name. Touch search followed by the button on the Search ● screen. Enter a search term using the Text Entry screen. The first ● few characters of the program name are sufficient. Touch Back to return to the previous screen followed by ●... -
Page 49: Updating The
uPDatInG tHe PrIme soFtWare Prime software can be updated by downloading the latest version from the Techne website www.techne. com. You will need a USB memory stick on which to save the update. Note that the update tool requires at least Windows XP SP2 and the Microsoft .Net Framework 3.5 SP1, which, if needed, can be downloaded from: http://www.microsoft.com/downloads/details.aspx?familyid=AB99342F-5D1A-413D-8319- 81DA479AB0D7&displaylang=en... -
Page 50: Upgrading From
uPGraDInG From X to rime rime PrimeX thermal cycler can be easily and quickly upgraded to the gradient PrimeG version by purchasing the 3PRIMEX/USB upgrade. The upgrade consists of a custom USB stick which will only work in a specified unit based on the unit serial number. -
Page 51: Faqs
FaQs How do I adjust the pressure of the heated lid for my tubes or plates? For thermal cyclers with lid adjustment knobs, rotate the knob anticlockwise until there is no pressure on the consumable, then close the lid and latch it. To obtain the correct pressure gently rotate the knob clockwise until it is possible to just feel the pressure being applied. -
Page 52: Technical Support And Servicing
tecHnIcal suPPort anD serVIcInG If you require further technical or application assistance please contact Techne at: E-mail: technehelp@bibby-scientific.com Phone: +44 (0)1785 810433 Fax: +44 (0)1785 810405 For servicing information please contact: Service Department Bibby Scientific Ltd. Beacon Road Stone Staffordshire ST15 0SA E-mail: service@bibby-scientific.com Phone: +44 (0)1785 810475... -
Page 53: Additional Information
aDDItIonal InFormatIon user maIntenance Before cleaning your unit, disconnect it from the power supply. The outer case of the Prime may be cleaned with a cloth dipped in water or ethanol (hexane or 50% methanol can also be used). No part of the case or cover should be immersed in the solvents. -
Page 54: Accessories
accessorIes The following accessories can be obtained from Bibby Scientific Ltd. or your Techne distributor: Product code Description 3PRIMEX/USB PrimeX gradient upgrade rePlacement Parts The following replacement parts can be obtained from Bibby Scientific Ltd. or your Techne distributor: Product code Description 3PRIMEBASE/02/B Prime block, 24 x 0.2ml... - Page 56 north & south america middle east Bibby Scientific Ltd. Bibby Scientific US Inc. Bibby Scientific Middle East Ltd. Beacon Road, Stone t/a Techne Inc. PO Box 27842, Engomi 2433 Staffordshire ST15 0SA 3 Terri Lane, Suite 10 Nicosia United Kingdom Burlington, NJ 08016 USA Cyprus Tel: +44 (0)1785 812121...
Need help?
Do you have a question about the Techne Prime Series and is the answer not in the manual?
Questions and answers