ibidi Silver 11921 Instruction Manual

Gas incubation system, gas incubation system, co2/o2

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Instruction Manual

ibidi Gas Incubation System – Silver Line

11921 ibidi Gas Incubation System, CO

11923 ibidi Gas Incubation System, CO

Version 2.0

/ O

ibi di Ga s Mi xe r

CO 2% Hu m%

Fl ow

75 .2

5. 1

9. 9

80 .0

5. 0

80 .0

5. 0

S[ 10 .0 ] Ru n

Se tu p

– Silver Line

/ O

– Silver Line

.com

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Summary of Contents for ibidi Silver 11921

Page 1 Instruction Manual ibidi Gas Incubation System – Silver Line ibi di Ga s Mi xe r ibi di Ga s Mi xe r CO 2% Hu m% CO 2% Hu m% Fl ow Fl ow 75 .2 75 .2 5. 1 5.
Page 3 Gas Incubation System – Silver Line Instruction Manual Contact ibidi GmbH Lochhamer Schlag 11 82166 Gräfelfing Germany Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-mail: info@ibidi.de Web: ibidi.com Version 2.0 (August 6, 2024)
Page 4 ........4.5.2 ibidi Heating System 4 Slides and ibidi Heating System Multiwell Plate – Silver Line: Removable Humidity Sensor .
Page 5 Gas Incubation System – Silver Line Instruction Manual 5.4.2 Incubation Parameters .......
Page 6 11921) and the ibidi Gas Incubation System, CO – Silver Line (product number 11923). This manual is written for use of the ibidi Gas Incubation System – Silver Line with the ibidi Heating System Slide/Dish – Silver Line (product numbers 12110, 12720, 12722, 12111), ibidi Heating Sys- tem 4 Slides –...
Page 7 Gas Incubation System – Silver Line Instruction Manual 1.3 Nomenclature The ibidi Gas Incubation System – Silver Line consists of the following three main components: ibidi Gas Mixe r CO CO2 % Hum % Flo w 75. 2 80. 0 S[ 10.
Page 8 Gas Incubation System – Silver Line Instruction Manual Table 1: (continued) Operating Conditions Operating site Indoor use only Operating temperature 18–30°C/64–86°F Operating humidity max. 80 % relative humidity (RH) Operating altitude max. 2000 m (atmospheric pressure 800–1060 hPa/11.6–15.4 psi) Storage Conditions -5–50°C/23–122°F...
Page 9 Resolution 0.1 % Control Control range 1–21 % (note: settings below 1 % are less accurate and a typical minimum value in an ibidi Incubation Chamber is 0.5 % O Accuracy ±0.2 % vol. (typical) ±0.7 % vol. (max.) Resolution 0.1 %...
Page 10 80 ml (approximately after 7 days at 10 l/h) 1.5 Disclaimer • ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of product use. • The contents of this manual are subject to change without notice for product improvement.
Page 11 • Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing equipment, and machine tools. • Avoid dust and corrosive gas. Do not install the ibidi Gas Incubation System – Silver Line where it could be exposed to high levels of dust or to outside air or ventilation outlets.
Page 12 Products manufactured by ibidi, unless otherwise specified, are warrantied for a period of one year from the date of shipment to be free of defects in materials and workmanship. If any defects in the product are found during this warranty period, ibidi will repair or replace the defective part(s) or product free of charge.
Page 13 (Restriction on the use of Hazardous Substances, RoHS). 1.10.1 EU Directive WEEE The ibidi Gas Incubation System – Silver Line must be disposed of in compliance with the WEEE Directive 2012/19/EC. This symbol on the product is in accordance with the European Union’s Waste Electrical and Elec- tronic Equipment (WEEE) Directive.
Page 14 Gas Incubation System – Silver Line Instruction Manual 1.11 Regulatory Statement EG-Konformitätserklärung EC Declaration of Conformity Wir / We ibidi GmbH Lochhamer Schlag 11 D-82166 Gräfelfing erklären hiermit die Übereinstimmung des genannten Produktes mit der Richtlinie 2014/35/EU - Niederspannungsrichtlinie und mit der Richtlinie 2014/30/EU über die Elektromagnetische Verträglichkeit.
Page 15 Instruction Manual 2 Intended Use The ibidi Gas Incubation System – Silver Line provides defined gas control for live cell imaging experi- ments. It is intended to be used with stage top incubators and can be applied on various microscopes.
Page 16 Humidified gas mix Feedback control Figure 2: Schematic view of the ibidi Stage Top Incubation System, combining the Heating System with the Gas Incubation System. RH: relative humidity. The Gas Incubation System provides a controlled atmosphere of gas (CO...
Page 17 4.1 Components of the ibidi Gas Incubation System – Silver Line An overview of the different ibidi Gas Incubation System – Silver Line versions is given in this Section. Table lists all available options of the ibidi Gas Incubation System – Silver Line and Table shows all included components.
Page 18 Gas Incubation System – Silver Line Instruction Manual Table 3: Overview of the components of the ibidi Gas Incubation System, CO – Silver Line and ibidi Gas Incubation System, CO – Silver Line. The drawings are not to scale.
Page 19 Gas Incubation System – Silver Line Instruction Manual In addition to the gas mixture, the humidity inside the Incubation Chamber is actively controlled by a feedback loop using a humidity sensor located in the Incubation Chamber. The humidity is increased by streaming the gas through a water column if the atmosphere is too dry.
Page 20 Gas Incubation System – Silver Line Instruction Manual 4.3 Humidifying Column To avoid evaporation and drying out of the sample, the Humidifying Column humidifies the gas stream flowing into the Incubation Chamber. Part of the mixed gas output is guided through the column. The water inside the column is warmed up for higher humidity uptake.
Page 21 Gas Incubation System – Silver Line Instruction Manual 4.4 Heated Tubing Package The Heated Tubing Package connects the gas output of the Gas Mixer to the Humidifying Column and to the Incubation Chamber on the microscope stage. To actively control the humidity, two gas outputs are needed.
Page 22 Removable Humidity Sensor The ibidi Heating System 4 Slides – Silver Line and the ibidi Heating System Multiwell Plate – Silver Line come with a removable humidity sensor that is fixed into the side wall of the Heated Lid of the Incubation Chamber (Figure 9).
Page 23 Gas Incubation System – Silver Line Instruction Manual Polymer block with humidity sensor Electrical connection via humidity sensor 12150, 12130, 12131 cable 12724, 12726, 12151 Figure 9: A: Incubation Chambers 4 Slides and Multiwell Plate with associated product numbers. B: Removable humidity sensor of the Incubation Chamber 4 Slides and the Incubation Chamber Multiwell Plate.
Page 24 Gas Incubation System – Silver Line Instruction Manual Polymer block with humidity sensor Spacer nut Through-hole Counter nut Figure 11: Installation of the removable humidity sensor to the side wall of the Heated Lid. CAUTION – Make sure that the polymer block is NOT in contact with the Heated Lid.
Page 25 Gas Incubation System – Silver Line together with the ibidi Heating System Slide/Dish – Silver Line and the ibidi Heating System 4 Slides – Silver Line or ibidi Heating System Multiwell Plate – Silver Line, respectively. The Gas Mixer and the Humidifying Column should be placed next to the microscope and on the same height level as the microscope stand.
Page 26 Computer (optional) Heated Tubing Package Figure 13: Connection of the components of the Gas Incubation System and the ibidi Heating System 4 Slides or ibidi Heating System Multiwell Plate. CAUTION – Do not place the Gas Mixer and Humidifying Column on the microscope stage! The vibration might disturb the measurement.
Page 27 Gas Incubation System – Silver Line Instruction Manual Heating Humidifying Column Bridge cable humidity sensor cable ibidi GmbH Am Klopfe rspitz 19 D-8215 2 Martin sried Germa ny +49(0) 89520 46 17 www.i bidi.co m ibid i Gas Mix er...
Page 28 Gas Incubation System – Silver Line Instruction Manual Table 5: Available connections and color code in the two different Gas Mixer versions Gas Input Gas Pressure Tubing Color from source with pressure reducer (labline / 1 bar/14.5 psi blue...
Page 29 Gas Incubation System – Silver Line Instruction Manual Figure 18: How to connect the gas tubing to a pressure reducing valve from a gas line, compressor, or gas bottle. A push-in fitting for 6 mm OD tubing is recommended.
Page 30 Incubation Chamber 4 Slides Figure 19: Connection of the Heated Tubing Package to the ibidi Gas Incubation System – Silver Line. CAUTION – Do not disconnect the Heated Tubing while the Gas Mixer is running. The resulting sudden change of pressure may damage the Gas Mixer.
Page 31 Temperature Controller and Gas Mixer. ibidi Heating System 4 Slides – Silver Line or ibidi Heating System Multiwell Plate – Silver Line If combining the ibidi Gas Incubation System – Silver Line with an Incubation Chamber 4 Slides of the ibidi Heating System 4 Slides –...
Page 32 Gas Incubation System – Silver Line Instruction Manual Incubation Chamber with Gas Mixer humidity sensor ibidi GmbH Am Klopferspitz 19 D-82152 Martinsried Heater Germany +49(0)89520 46 17 0 www.ibidi.com ibidi Gas Mixer Type: Serial number: ibiGM2-001 Production date: 11/2018...
Page 33 Gas Incubation System – Silver Line Instruction Manual 5.2 (Re)-Filling the Humidifying Column Before the first use, the Humidifying Column must be filled with 130 ml of ultra-pure water. During operation, the column should always be refilled before the water level falls below the minimum mark.
Page 34 Column (max. 60°C/140°F) and the Heated Tubing Package (max. 50°C/122°F outside). To turn off the ibidi Gas Incubation System, first switch off the Gas Mixer and then close all sources for gas supply. 5.4 Setting Parameters With the Front Display All control parameters can be manually set on the controller using the buttons and the display on the front panel.
Page 35 “Run” (= system is running) or “Stop” (= system is not running) (Figure 24). Figure 24: Main display indicating “Run”. 5.4.2 Incubation Parameters Table 7: Recommended parameters for the operation of the ibidi Gas Incubation System – Silver Line Parameter Recommended Values 5–10 % depending on buffer...
Page 36 Gas Incubation System – Silver Line Instruction Manual 5.4.2.4 O regulation/hypoxia (Gas Mixer for CO only) To generate hypoxia conditions, set the oxygen to the target value. The O will be replaced by nitrogen (N ) until the set value is reached.
Page 37 Gas Incubation System – Silver Line Instruction Manual 3. Select “On” or “Off” and confirm with the round button. 4. To move on navigate to “Return” and press the round button. Table 8: Channel numbers are dedicated as follows:...
Page 38 Gas Incubation System – Silver Line Instruction Manual 6 IncuControl Software The Gas Mixer has a USB interface for computer control and data logging. For this purpose, ibidi provides the IncuControl software version (3.x.x) that can be downloaded from the ibidi website directly via ibidi.com/stagetop.
Page 39 Gas Incubation System – Silver Line Instruction Manual 7 Maintenance 7.1 Disinfection and Cleaning 7.1.1 Wiping the Outer Surface To clean the Gas Mixer and Humidifying Column from the outside, switch off the Gas Mixer and disconnect all cables and tubing. Leave the instrument to cool down for approximately 5 minutes. Use only dry cloth or a cloth dampened with water (ultrapure) or common lab disinfection solutions that are based on quaternary ammonium compounds (e.g., Barrycidal 36 or Pharmacidal).
Page 40 Gas Incubation System – Silver Line Instruction Manual 8 Troubleshooting 8.1 Controller Display Errors Error Description Possible Cause Possible Solution The display does not work, although Power supply inter- Check the power supply the power supply is connected. rupted.
Page 41 Gas Incubation System – Silver Line Instruction Manual 8.2 Flow Readings Error Description Possible Cause Possible Solution Gas flow reading is “0”. Gas supply lines are not Open gas lines. opened. Gas supply lines are not Inspect all gas supply lines connection connected correctly.
Page 42 Gas Incubation System – Silver Line Instruction Manual 8.3 CO Channel Readings Error Description Possible Cause Possible Solution channel shows “0”. gas supply line is Open gas lines. closed. gas supply line is Inspect CO gas line to ensure gas supply not connected correctly.
Page 43 Gas Incubation System – Silver Line Instruction Manual 8.4 O Channel Readings Error Description Possible Cause Possible Solution channel shows Dead end tubing of the Open the dead-end tubing for the N “20 %” or higher, even Gas supply channel supply.
Page 44 Gas Incubation System – Silver Line Instruction Manual 8.5 Humidity Channel Readings Error Description Possible Cause Possible Solution The humidity does Incubation Chamber Make sure the Incubation Chamber is closed not reach set value. open or not closed properly.
Page 45 Gas Incubation System – Silver Line Instruction Manual (Table continued) Condensation Remove water with a lint free tissue from hu- the humidity sensor. midity sensor and allow the sensor to dry. If set humidity is close to 100 %, reduce set value by about 5–10 %.
Page 46 Depending on the incubating conditions, small volumes might evaporate quickly, especially during long-term experiments. If you have an actively controlled humidifying device (e.g. ibidi Gas Incubation System), increase the set value for humidity. Additionally, we suggest using silicone oil (e.g. Anti- Evaporation Oil, ibidi, 50051) to decrease evaporation.
Page 48 GmbH Lochhamer Schlag 11 82166 Gräfelfing Germany Toll free within Germany: Phone: 0800 / 00 11 11 28 Fax: 0800 / 00 11 11 29 International calls: Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-Mail: info@ibidi.com...

This manual is also suitable for:

Silver 11923