ibidi m–Slide VI 0.4 Series Instructions

Instructions
Material
ibidi µ–Slides, µ–Dishes, and µ–Plates are made of a plas-
tic that has the highest optical quality. The polymer cov-
erslip on the bottom exhibits extremely low birefringence
and autofluorescence, similar to that of glass. Also, it is
not possible to detach the bottom from the upper part.
The µ–Slides, µ–Dishes, and µ–Plates are not autoclavable,
since they are only temperature–stable up to 80°C/175°F.
Please note that gas exchange between the medium and
incubator's atmosphere occurs partially through the poly-
mer coverslip, which should not be covered.
Optical Properties ibidi Polymer Coverslip
Refractive index n (589 nm)
D
Abbe number
Thickness
Material
Please note! The ibidi polymer coverslip is compatible
with certain types of immersion oil only. A list of suit-
able oils can be found on page 3.
Shipping and Storage
The µ–Slides, µ–Dishes and µ–Plates are sterilized and
welded in a gas-permeable packaging. The shelf life under
proper storage conditions (in a dry place, no direct sun-
light) is listed in the following table.
Conditions
Shipping conditions
Storage conditions
0.4
µ–Slide VI
µ–Slide VI
The ibidi product family is comprised of a variety of µ–Slides and µ–Dishes, which have all
been designed for high–end microscopic analysis of fixed or living cells. The high optical
quality of the material is similar to that of glass, so you can perform all kinds of fluorescence
experiments with uncompromised resolution and choice of wavelength.
The convenient six channel format of the µ–Slide VI
the application of standard immunofluorescence protocols, like treatment, staining, and mi-
croscopy of living or fixed cells. Alternatively, the µ–Slide VI
and enables you to observe cells under flow conditions.
1.52
56
No. 1.5 (180 µm)
polymer coverslip
Ambient
RT (15-25°C)
0.4
0.4
Shelf Life of Different Surfaces
ibiTreat, Glass Bottom, ESS
Collagen, Poly-Lysine
Fibronectin
Geometry of the µ–Slide VI
0.4
The µ–Slide VI provides a standard slide format accord-
ing to ISO 8037/1. The lateral adapter to adapter distance
of 9 mm (like 96 well plates) allows using multichannel
pipettes.
Number of Channels
Channel volume
Channel length
Channel width
Channel height
Adapters
Volume per reservoir
Growth area
Coating area using 30 µl
Bottom matches coverslip
µ–Slide Surfaces
Depending on the type of cells and the special application
you are using, you will need µ–Slides with different sur-
faces. If you do not require any special adhesion molecules
for your application, the best choice will be ibiTreat, a tis-
sue culture treated surface.
The uncoated µ–Slide is manufactured from hydrophobic
plastic. For the cultivation of most cell lines, it is indis-
pensable to treat the uncoated µ–Slide with biopolymers,
which mediate cell adhesion and growth.
Page 1
is ideal for static cell cultivation and
0.4
can be connected to a pump
36 months
18 months
4 months
0.4
Dimensions
6
30 µl
17 mm
3.8 mm
0.4 mm
female Luer
60 µl
2
0.6 cm per channel
2
1.2 cm per channel
No. 1.5
Version 3.0 (2015-12-10)