ibidi Glass Bottom Dish Instructions

Instructions
The ibidi product family is comprised of a variety of µ–Slides and µ–Dishes, which have all been
designed for high–end microscopic analysis of fixed or living cells.
The glass bottom versions of the µ–Slides and µ–Dishes are especially designed for TIRF, super reso-
lution and single molecule applications.
The Glass Bottom Dish 35 mm is a standard glass bottom dish for cell culture and high resolution
microscopy.
Material
35 mm
The Glass Bottom Dish
Dish, but with a glass coverslip bottom. It is not possible to
detach the bottom. The Dishes are not autoclavable since
they are temperature stable only up to 80°C / 175°F.
Optical Properties Glass Bottom Dish
Refractive index n 1.523
D
Abbe number 55
Thickness No. 1.5
(170 µm (+20 µm/-10 µm))
Material Schott borosilicate glass,
D 263M
Attention!
Be cautious when handling ibidi labware products
with glass bottom! The glass coverslip or glass slide
is very fragile and might break easily. Handle with
care to avoid physical injury and damage to devices
through leakage of the medium.
Geometry
Geometry of the Glass Bottom Dish
Diameter dish
Volume
Growth area
Coating area using 400 µl
Diameter observation area
Height with / without lid
Bottom
35 mm
Glass Bottom Dish
Glass Bottom Dish
is made of a standard 35 mm
35 mm
35 mm
35 mm
2 ml
2
3.14 cm
2
3.7 cm
20 mm
11.7 mm/9.9 mm
Glass coverslip No. 1.5
35 mm
Surface and Coating
The Glass Bottom Dish
coated glass coverslip. Washing steps (e.g. with PBS) be-
fore cell seeding can remove glass dust which is advanta-
geous for direct cell growth on the surface.
Protein coatings increase direct cell growth of adherent
cells. Specific coatings on glass are possible following this
protocol:
• Prepare your coating solution according to the man-
ufacturer's specifications or reference. Prepare your
Glass Bottom Dish
to a coating area of 3.7 cm
400 µl.
• Apply 400 µl into the growth area. Make sure that
the entire bottom is covered with liquid easily tilting
or shaking the µ–Dish. Put on the lid and leave at
room temperature for at least 30 minutes.
• Aspirate the solution and wash. Optionally, let dry
at room temperature.
Detailed information about coatings is provided in Appli-
cation Note 08 "Cell culture
Shipping and Storage
The µ–Slides, µ–Dishes and µ–Plates are sterilized and
welded in a gas-permeable packaging. The shelf life under
proper storage conditions (in a dry place, no direct sun-
light) is listed in the following table.
Shipping conditions
Storage conditions
Glass Bottom
Page 1
35 mm
is manufactured with an un-
35 mm
. Adjust the concentration
2
and a coating volume of
coating".
Conditions
Ambient
RT (15-25°C)
Shelf Life
36 months
Version 1.1 (2018-07-11)