Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for ibidi 12150
Summary of Contents for ibidi 12150
- Page 1 Gl as s Li d Pl at e 36 .9 42 .1 I 38 .0 37 .0 42 .0 S[ 38 .0 ] Ru n Se tu p N. C. 12150 ibidi Heating System Multiwell Plate – Silver Line .com Version 1.0...
- Page 2 Heating System Multiwell Plate – Silver Line Version 1.0 (August 1, 2022) Instruction Manual...
- Page 3 Heating System Multiwell Plate – Silver Line Contact ibidi GmbH Lochhamer Schlag 11 82166 Gr¨ a felfing Germany Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-mail: info@ibidi.de Internet: ibidi.com...
-
Page 4: Table Of Contents
2 Intended Use 3 Principle 4 Equipment 4.1 Components of the ibidi Heating System Multiwell Plate ....4.2 Stage Top Incubator Overview ....... . - Page 5 ..... . 5.2 Start Operation of the ibidi Heating System Multiwell Plate ....
-
Page 6: Preamble
1 Preamble 1.1 Introduction This manual is your guide to using the ibidi Heating System Multiwell Plate – Silver Line for cell cul- ture experiments on an optical microscope. It instructs first-time users on how to use the instrument, and serves as a reference for experienced users. -
Page 7: Nomenclature
Where adequate, the ibidi Heating System Multiwell Plate – Silver Line is hereafter abbreviated and referred to as ibidi Heating System Multiwell Plate in body text and figure captions. The ibidi Tem- perature Controller – Silver Line is hereafter abbreviated and referred to as Temperature Controller, where adequate. - Page 8 Heating System Multiwell Plate – Silver Line Table 1: (continued) Operating humidity max. 80% relative humidity (RH) Operating altitude max. 2000 m (atmospheric pressure 800–1060 hPa/11.6–15.4 psi) Storage conditions -5–50°C/23–122°F, humidity 60% relative humidity (RH) Outer Dimensions and Characteristics of the Components ibidi Temperaure Controller –...
-
Page 9: Disclaimer
160 mm × 110 mm 1.5 Disclaimer • ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of product use. • The contents of this manual are subject to change without notice for product improvement. - Page 10 • Only use extension cables that have a protective ground wire. • Do not operate the ibidi Heating System Multiwell Plate under conditions that pose a risk of explosion, implosion, or the release of gases.
-
Page 11: Limited Warranty
1.8 Transporting the ibidi Heating System Multiwell Plate The weight of the ibidi Temperature Controller – Silver Line is approx. 1.75 kg/3.86 lbs. Moving the ibidi Temperature Controller – Silver Line during operation can pose a risk of personal injury or damage to the instrument. -
Page 12: Repairing The Ibidi Heating System Multiwell Plate
– Do not try to repair the ibidi Heating System Multiwell Plate by yourself. Disassembly of the ibidi Heating System Multiwell Plate is not allowed. Disassembly poses a risk of personal injury or damage to the devices. Contact ibidi technical support if there is a need to disassemble a device. -
Page 13: Regulatory Statement
Heating System Multiwell Plate – Silver Line 1.11 Regulatory Statement EG-Konformitätserklärung EC Declaration of Conformity Wir / We ibidi GmbH Lochhamer Schlag 11 D-82166 Gräfelfing erklären hiermit die Übereinstimmung des genannten Produktes mit der Richtlinie 2014/35/EU - Niederspannungsrichtlinie und mit der Richtlinie 2014/30/EU über die Elektromagnetische Verträglichkeit. -
Page 14: Intended Use
Gas Incubation System to regulate the CO and O concentrations as well as the humidity. The ibidi Heating System Multiwell Plate is for research use only! It is not intended for use in diag- nostic procedures. 3 Principle Physiological Conditions in Live Cell Imaging Cells react sensitively to changes in their environment. - Page 15 Humidified gas mix regulation Feedback control Figure 2: Schematic view of the ibidi Stage Top Incubation System, combining the Heating System with the Gas Incubation System. RH: relative humidity. The Gas Incubation System provides a controlled atmosphere of gas (CO...
- Page 16 Heating System Multiwell Plate – Silver Line Heated Lid 40°C 37°C Heated Plate Heated Glass Bottom No condensation Unheated Lid 34°C 37°C Heated Plate Heated Glass Bottom Condensation Figure 4: The Heated Lid prevents condensation effects on the lid of the cell culture vessel.
-
Page 17: Equipment
Heating System Multiwell Plate – Silver Line 4 Equipment This section provides a brief overview over all parts of the ibidi Heating System Multiwell Plate including a description of the characteristics. 4.1 Components of the ibidi Heating System Multiwell Plate The components of the ibidi Heating System Multiwell Plate are listed below. -
Page 18: Stage Top Incubator Overview
4.2 Stage Top Incubator Overview The parts of the ibidi Heating System Multiwell Plate are set up as a stage to incubator as shown in Figure 5. The ibidi Heating System Multiwell Plate can be combined with the ibidi Gas Incubation System –... - Page 19 Heating System Multiwell Plate – Silver Line 4.3 ibidi Temperature Controller – Silver Line The ibidi Temperature Controller – Silver Line is designed to precisely operate the different ibidi Silver Line heating devices, including the Incubation Chamber Multiwell Plate – Silver Line. In total, 6 channels are available, each channel accommodating one heated component (e.g., the Heated Lid,...
-
Page 20: Heated Plate
Figure 7: Rear view of the ibidi Temperature Controller – Silver Line. 4.4 Heated Plate The Heated Plate provides the base for the ibidi Heating System Multiwell Plate. The lower part of the Heated Plate fits into a mechanical or scanning stage with opening for mounting frames K (160 mm ×... -
Page 21: Heated Lid
Heating System Multiwell Plate – Silver Line The Heated Plate can be leveled to compensate for uneven samples. Use the 0.9 mm INBUS/Hex Key to adjust the M2 set screws at each of the plate’s corners. Figure 9: Leveling of the Heated Plate. - Page 22 Heating System Multiwell Plate – Silver Line 4.6 Cable Clip for Optional Use CAUTION – ibidi GmbH does not accept any liability for damages resulting from the use of the cable clip on your microscope. Please check with your microscope provider and/or your institution’s technical support if using the cable clip on the microscope stage...
-
Page 23: Operation
Heating System Multiwell Plate – Silver Line 5 Operation Before starting an experiment, check that the ibidi Heating System Multiwell Plate fits on your mi- croscope stage, and that your cell culture vessels are compatible with the Holder. Connect all parts (Section 5.1) and perform a temperature adjustment as explained in Section 5.4. -
Page 24: Start Operation Of The Ibidi Heating System Multiwell Plate
5.2 Start Operation of the ibidi Heating System Multiwell Plate The ibidi Temperature Controller – Silver Line is switched on by the dip switch at the back. Make sure the display shows “RUN” (see Section 5.3.1). The system immediately starts heating up the heated devices (Heated Plate, Heated Lid). -
Page 25: Run/Stop Mode
Depending on the heated components used, different connector ports (output channels) at the back of the ibidi Temperature Controller – Silver Line can be used (Table 4). Accordingly, the information of one, two, or three heated components with the designations Plate, Lid, Glass or Obj is shown per display. -
Page 26: Select Temperature Sensor
“Setup” entry and pressing the round button. The mode dialog will open. Mode Each channel of the ibidi Temperature Controller – Silver Line can be set to an “On” or “Off” state. For standard operation all channels must be turned on. -
Page 27: Temperature Adjustment In The Sample
Heated Plate. To ensure that the sample reaches the correct temperature, ibidi recommends a temperature adjustment step before starting the first experiment. The temperature adjustment should also be done separately for each chamber type used. - Page 28 2. Connect the external temperature sensor (thermocouple type K) to the plug at the back of the ibidi Temperature Controller – Silver Line. The connected external temperature sensor will appear in the display. 3. Fill the provided culture vessel (multiwell plate) with water or medium. Volumes should be the same amount you need for your experiments.
-
Page 29: Sample Preparation
– Silver Line or ibidi Heating System 4 Slides – Silver Line) 5.5 Sample Preparation Check that the cell cultureware you intend to use fits into Heated Plate of the ibidi Heating System Multiwell Plate. Prepare the cells according to your protocol and place the multiwell plate into the Heated Plate mounted on the microscope stage. -
Page 30: Incucontrol Software
Heating System Multiwell Plate – Silver Line 6 IncuControl Software The ibidi Temperature Controller – Silver Line has a USB interface for computer control and data logging. For this purpose, ibidi provides the IncuControl software that comes with the controller or... -
Page 31: Maintenance
7 Maintenance 7.1 Disinfection and Cleaning The heated components and the ibidi Temperature Controller – Silver Line do not have to be sterile, because the incubation chamber has no direct contact with the cells and the cell culture medium. If disinfection is necessary for some reason, we recommend using isopropanol (70%) or common lab disinfection solutions based on quaternary ammonium compounds (e.g., Barrycidal 36 or Pharmaci-... -
Page 32: Troubleshooting
Depending on the incubating conditions, small volumes might evaporate quickly, especially during long-term experiments. If you have an actively controlled humidifying device (e.g., ibidi Gas Incu- bation System), increase the set value for relative humidity. Additionally, we suggest using silicone oil (e.g., Anti-Evaporation Oil, ibidi, 50051) to decrease evaporation. - Page 33 Heating System Multiwell Plate – Silver Line Instruction Manual Version 1.0 (August 1, 2022)
- Page 34 Heating System Multiwell Plate – Silver Line Version 1.0 (August 1, 2022) Instruction Manual...
- Page 35 Heating System Multiwell Plate – Silver Line Instruction Manual Version 1.0 (August 1, 2022)
- Page 36 GmbH Lochhamer Schlag 11 82166 Gräfelfing Germany Toll free within Germany: Phone: 0800 / 00 11 11 28 Fax: 0800 / 00 11 11 29 International calls: Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-Mail: info@ibidi.com...
Need help?
Do you have a question about the 12150 and is the answer not in the manual?
Questions and answers