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Document: 333D042B
Subject: Rev. B
OPERATOR'S MANUAL
SterilGARD
SG403A / SG603A with 12" SASH OPENING
Animal Transfer Station (ATS)
Waste Disposal Unit (WDU)
This manual includes information for installation, operation, maintenance and spare parts. We
recommend that it be kept near the cabinet for ready reference.
T
B
HE
AKER
Class II, Type A1
Biological Safety Cabinet
MODEL
CONFIGURATIONS
Necropsy
C
OMPANY
®
III Advance°
Aug-16-2007
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Summary of Contents for Baker SterilGARD III
- Page 1 Document: 333D042B Aug-16-2007 Subject: Rev. B AKER OMPANY OPERATOR’S MANUAL ® SterilGARD III Advance° Class II, Type A1 Biological Safety Cabinet MODEL SG403A / SG603A with 12” SASH OPENING CONFIGURATIONS Animal Transfer Station (ATS) Waste Disposal Unit (WDU) Necropsy This manual includes information for installation, operation, maintenance and spare parts. We recommend that it be kept near the cabinet for ready reference.
- Page 2 OMPANY INTRODUCTION AND WELCOME It is a pleasure to welcome you to the growing number of customers who own and operate Baker biological safety cabinets. As the inventors of the laminar flow biological safety cabinet and the leaders in the field, Baker people take special pride in providing a cabinet that is designed for maximum performance.
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Page 3: Table Of Contents
Document: 333D042B Aug-16-2007 Subject: Rev. B TABLE OF CONTENTS ® I - FUNCTION OF THE STERILGARD III ADVANCEº............4 Airflow Inside the Biological Safety Cabinet ......................4 Unique features that contribute to airflow performance..................4 Fig. 1 Airflow Inside Cabinet ..........................5 Base Features.................................6 Cabinet Pressure Plenums ............................7 Design Details................................7 Motor/ blower capacity............................7... - Page 4 REPLACEMENT PARTS LIST ..........................42 HYDRAULIC LIFT STAND OPERATION.......................43 Things to remember when using your SterilGARD III w/ Hydraulic Lift Stand: ..........43 Lowering the Hydraulic Lift Stand to the lowest position (ATS & Necropsy) ...........43 Lowering the Hydraulic Lift Stand to the lowest position (Waste Disposal Unit) ..........44 Dimensional Drawing –...
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Page 5: I - Function Of The Sterilgard ® Iii Advanceº
The Baker high-velocity return air slots are located along the side walls of the work area opening. Air is drawn into the slots at very high speed, preventing the escape of particulates and ensuring that no unfiltered air enters the work area. - Page 6 Exhaust Document: 333D042B Aug-16-2007 Subject: Rev. B Exhaust HEPA filter = HEPA filtered air = Contaminated air Into blower = Room air Positive pressure plenum Down flow HEPA filter Work area Intake (Room air) Work surface Fig. 1 Airflow Inside Cabinet - 5 -...
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Page 7: Base Features
Document: 333D042B Aug-16-2007 Subject: Rev. B Base Features • Performs to NSF 49 standard for Class II, Type A1 biosafety cabinet. • HEPA filtration of air before it enters the work area. • Front accessibility to electrical components, lamp, blower, and filters. •... -
Page 8: Cabinet Pressure Plenums
Document: 333D042B Aug-16-2007 Subject: Rev. B Cabinet Pressure Plenums The cabinets work area is surrounded by negative pressure and all external seals are under negative pressure. All internal positive pressure plenum seals are surrounded by negative pressure plenums. Design Details Motor/ blower capacity A motor/blower's capacity is measured by its ability to provide a nearly constant volume of air as resistance increases because of filter loading. -
Page 9: All-Metal Plenums
Document: 333D042B Aug-16-2007 Subject: Rev. B plenums. Acting as a protective screen, integral to the interior walls, they help prevent wipes and other paper materials from being drawn into the blower system. They need to be kept clean at all times. All-metal plenums The plenums are constructed entirely of carbon and stainless steel in order to provide strength, durability, air-tightness and resistance to deterioration. -
Page 10: Motor / Blower Assembly
Document: 333D042B Aug-16-2007 Subject: Rev. B filter changing. Motor / Blower assembly The motor and blower are assembled on a slide plate. This allows the assembly to be easily removed from the positive pressure plenum for faster servicing or replacement. Cable ports (Optional) A cable port is located in the cabinet’s left and right side walls. -
Page 11: Specifications
Document: 333D042B Aug-16-2007 Subject: Rev. B Specifications Weight Model CABINET WEIGHT SHIPPING WEIGHT SG403A-ATS 700 lbs [3114 N] 1,200 lbs [5338 N] SG603A-ATS 900 lbs [4003 N] 1,400 lbs [6228 N] SG403A-WDU 700 lbs [3114 N] 1,200 lbs [5338 N] SG603A-WDU 900 lbs [4003 N] 1,400 lbs [6228 N]... -
Page 12: Environmental Conditions
The cabinet is provided with an optional lift that requires 115V AC at 2 Amps intermittent duty. The lift duty cycle is 1 minute on, 9 minutes off. ® The cabinet incorporates the Baker StediVOLT blower motor speed control. This compensates for normal variations in incoming line voltage. -
Page 13: Hydraulic Lift Stand
Document: 333D042B Aug-16-2007 Subject: Rev. B Hydraulic Lift Stand The hydraulic lift stand provides the following range of heights: Work Knee Space Overall Surface Height Height Height Minimum Setting ” ” 81 ¾” Maximum Setting ” ” 100 ¾” The cabinets have an optional adjustable hydraulic lift stand to adjust the work surface height to accommodate the ergonomic requirements of any user, standing or sitting. -
Page 14: Advance Ofor Use
Published research from The Baker Company and unpublished tests performed at the National Cancer Institute show that if a draft or other disruptive air current were to exceed the intake velocity of the cabinet, contamination can enter the work area or escape from it. [For more information, contact Baker] - 13 -... -
Page 15: Installing The Cabinet
Document: 333D042B Aug-16-2007 Subject: Rev. B If the cabinet exhausts air into the laboratory instead of venting to the outside, it is important that there is adequate space between the top of the cabinet and the ceiling. A solid ceiling located too close to the exhaust filter will restrict the air and limit the intake velocity. -
Page 16: Connecting The Exhaust For Ducting Outdoors
Document: 333D042B Aug-16-2007 Subject: Rev. B Model Sash Opening Exhaust Volume SG403A-ATS 12” [304.8mm] 365 CFM (+/-5%) [172 L/sec] 12” [304.8mm] SG603A-ATS 554 CFM (+/-5%) [261 L/sec] 12” [304.8mm] SG403A-WDU 365 CFM (+/-5%) [172 L/sec] 12” [304.8mm] SG603A-WDU 554 CFM (+/-5%) [261 L/sec] 12”... -
Page 17: Final Connections And Tests
You may want to install an indicator light or some other safety device to give warning if the exhaust system fails. For more details, please contact The Baker Company. While the cabinet contains an internal damper to adjust for variations in filter resistance and sash opening size, the building exhaust system should contain provisions to adjust the building system flow and pressure. - Page 18 For additional start up and use procedures, please turn to Section 3, "Proper Cabinet Use." FOR MORE INFORMATION For a complete listing of articles, papers and reports related to containment, clean air products and safety, contact The Baker Company for our complete Bibliography or visit our website at www.bakerco.com.
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Page 19: Iii - Proper Cabinet Use
In order to keep the interior work area clean and free of particulates, all Baker biosafety cabinets are designed for continuous operation. If the blower is turned off, the work area will become contaminated with room air. -
Page 20: Programmable Delay Off Time Function
Document: 333D042B Aug-16-2007 Subject: Rev. B • Ultraviolet (UV) Light On / Off [Optional] – A bulb, which produces light in the ultraviolet range, may be used to help disinfect the work area. This switch controls the UV Light inside the work area if the UV Light option is installed. -
Page 21: Start-Up Procedure
Document: 333D042B Aug-16-2007 Subject: Rev. B 1 hour increment programming: 1. Press and hold the pushbutton of the device you want to program. 2. In about 3 seconds you will hear a short ‘beep’. Continue to hold the pushbutton. In about an additional 3 seconds you will hear a longer ‘beep’. -
Page 22: Working In The Cabinet
Document: 333D042B Aug-16-2007 Subject: Rev. B Working in the Cabinet 1. Hands and arms should be washed thoroughly with germicidal soap both before and after work in the cabinet. Operators are encouraged to wear long-sleeved gowns or lab coats with tight-fitting cuffs and sterile gloves. -
Page 23: Reacting To Spills
Document: 333D042B Aug-16-2007 Subject: Rev. B surfaces should be repeated. Check the work area carefully for spilled or splashed nutrient that might support bacterial growth. Never use the cabinet to store supplies or laboratory equipment. 11. We recommend that the cabinet be left running continuously to ensure containment and cleanliness. If the user elects to turn the cabinet off at the end of a work session, the window should be closed completely. -
Page 24: Waste Disposal Unit
Document: 333D042B Aug-16-2007 Subject: Rev. B Waste Disposal Unit The waste disposal system utilizes a double bagging process. The inner bag is connected to a removable collar that is located at the cabinet’s work surface. The outer bag is connected to a fixed collar located under the cabinet’s drain pan. -
Page 25: Decontamination Procedure
Document: 333D042B Aug-16-2007 Subject: Rev. B in a visible and nearby location. The volumes of the SG403A & SG603A cabinets are 49 and 78 cubic feet respectively. Provide the correct amount of decontaminating gas for this volume. Carcinogens and other toxins present a unique chemical deactivation problem and the standard biological decontamination will not, of course, be effective against chemicals or other non-biological materials. -
Page 26: Cleaning And Disinfecting Stainless Steel
Document: 333D042B Aug-16-2007 Subject: Rev. B front access opening. This will promote the even distribution of formaldehyde gas throughout the cabinet. 9. If the cabinet is vented to the outside, then the exhaust transition should have a means to recirculate the exhaust airflow back to the cabinet blower. -
Page 27: Simple Cleaning
Document: 333D042B Aug-16-2007 Subject: Rev. B Simple Cleaning IMPORTANT Do not use steel wool or steel pads when cleaning stainless steel. Dirt deposits on stainless steel (dust, dirt and finger marks) can easily be removed. Frequently, warm water, with or without detergent, is sufficient. If this does not remove the deposits, mild, non-abrasive household cleaners can be used with warm water and bristle brushes, sponges or clean cloths. -
Page 28: About The Hepa Filters
Proven efficiency in all HEPA filters used in Baker cabinets are 99.99% for particles 0.3 microns in diameter. The 0.3 micron particle is used as the basis for filter definition because theoretical studies have shown that filtration efficiency should be at a minimum for particles of this diameter, with efficiency increasing for particles either larger or smaller. -
Page 29: Iv - Onsite Checks And Maintenance Procedures
Our representatives can tell you about other tests, which you may consider desirable. As reported earlier in this manual, each individual cabinet made by The Baker Company is carefully tested before it leaves the factory. Your copy of the test report, which you will find at the back of this ®... - Page 30 Document: 333D042B Aug-16-2007 Subject: Rev. B 3” Window Opening Area Correction Factor 8” Window Opening Area Example: SterilGARD 403A with 8” sash opening 3. Finally, a correction factor is added to this value which again is noted on the airflow data plate affixed to the front of your cabinet.
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Page 31: Filter Leak & Smoke Testing
Document: 333D042B Aug-16-2007 Subject: Rev. B the airflow cannot be maintained, it will be necessary to replace the HEPA filters. (See "Procedure for HEPA Filter Replacement" later in this section). Filter Leak & Smoke Testing The filters in your cabinet were tested at our factory before shipment to ensure that the media, gasket, and frame were not exceeding NSF International Standard 49 allowable leak rates. -
Page 32: Airflow Smoke Pattern Test
Document: 333D042B Aug-16-2007 Subject: Rev. B cabinet. WARNING If the cabinet has been in use then it must be decontaminated before removing the plug. 4. Introduce the aerosol challenge to the cabinet blower at the front work surface perforation. 5. Perform filter leak test per NSF 49. 6. -
Page 33: Replacing The Hepa Filters
Document: 333D042B Aug-16-2007 Subject: Rev. B Replacing the HEPA Filters When the certifier can no longer set the cabinet air flows to within ±5% FPM of the nominal set point by adjusting the speed control and/or balancing damper then it may mean the filters are loaded with particles and must be replaced. - Page 34 Document: 333D042B Aug-16-2007 Subject: Rev. B face down inside the light canopy. (No electrical connections should need to be disconnected) 7. Loosen each of eight (10) 3/8” hex head bolts holding the front access panel. Be careful not to damage or loose the seal washer on each bolt when removing them.
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Page 35: Changing The Down Flow Filter
Document: 333D042B Aug-16-2007 Subject: Rev. B Changing the down flow filter 1. Loosen the two (2) ½” hex head down flow plenum clamping bolts located on either side of the down flow plenum, alternating two or three revolutions at a time until the plenum is raised about ¼” [6.4mm]. - Page 36 Document: 333D042B Aug-16-2007 Subject: Rev. B WARNING Once removed, the filter should immediately be sealed in a chemical hazard bag appropriately marked for the type of hazard and then disposed of safely in accordance with environmental regulations. 3. Slide the new exhaust filter in all the way in until it stops against the rear filter stop. Be careful not to contact the filter media with your fingers.
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Page 37: Troubleshooting
Document: 333D042B Aug-16-2007 Subject: Rev. B Troubleshooting Here are some suggestions based on our experience with the use and misuse of biological safety cabinets. When a smoke test indicates that there is air flowing from the interior of your cabinet into the surrounding room- 1. - Page 38 Document: 333D042B Aug-16-2007 Subject: Rev. B qualified electrician, using the wiring diagram in the Appendix of this manual, can check this breaker. If the Ultraviolet (UV) Light doesn’t work- 1. Make sure the window is completely closed. 2. The Yellow indicator below the switch should be On and the Fluorescent Light should turn Off automatically.
- Page 39 Document: 333D042B Aug-16-2007 Subject: Rev. B 2. Towels or wipes are blocked the towel guards in the drain pan area. 3. The viewscreen is in the closed position and the in-house exhaust fan is still pulling air through the cabinet. 4.
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Page 40: Sg403A/Sg603A Disassembly Instructions
Document: 333D042B Aug-16-2007 Subject: Rev. B SG403A/SG603A DISASSEMBLY INSTRUCTIONS NOTES: Refer to diagram on Page 28 for blower, filter and plenum configuration. For Motor/Blower removal, follow steps 1-18. For filter removal, follow steps 1-15 & 21-23 TOOLS REQUIRED: (1) 3/8" Spin Wrench (2) 3/8" Socket and Drill (3) 3/4" Wrench and (4) 1/4" Spin Wrench DISASSEMBLY: Disconnect Power Source! -
Page 41: Reassembly
Document: 333D042B Aug-16-2007 Subject: Rev. B Remove the 3/4" brass nuts from each petcock plumbing connection. Repeat the process on the other side, if plumbing exits. Lift out the worksurface using the pull tabs near unit sidewalls. REASSEMBLY: Use the disassembly instructions in reverse order. -
Page 42: Appendix
Document: 333D042B Aug-16-2007 Subject: Rev. B Appendix... -
Page 43: Replacement Parts List
Document: 333D042B Aug-16-2007 Subject: Rev. B REPLACEMENT PARTS LIST * = Note: Both GE and Baldor motors are qualified for this product. Please determine which brand you have prior to ordering replacement parts Part Name Part No. Part No. SG403A SG603A *Capacitor *11558... -
Page 44: Hydraulic Lift Stand Operation
Things to remember when using your SterilGARD III w/ Hydraulic Lift Stand: Your SterilGARD III lift stand is designed for mobility in and between rooms. It comes with 5” diameter casters that have brakes. When attempting to move the cabinet, be sure to check that the brakes are “off”. -
Page 45: Lowering The Hydraulic Lift Stand To The Lowest Position (Waste Disposal Unit)
Document: 333D042B Aug-16-2007 Subject: Rev. B facility. Once you have moved your cabinet into its desired location, press and hold the up arrow until the cabinet reaches a comfortable working height. (Note: Again, please check for any obstructions above the cabinet that may damage the unit while being raised to the upper position.) Your cabinet is now ready to use. -
Page 46: Dimensional Drawing - Standard Configuration [Sg403A-Ats]
Document: 333D042B Aug-16-2007 Subject: Rev. B Dimensional Drawing – Standard Configuration [SG403A-ATS]... -
Page 47: Dimensional Drawing - Standard Configuration [Sg603A-Ats]
Document: 333D042B Aug-16-2007 Subject: Rev. B Dimensional Drawing – Standard Configuration [SG603A-ATS]... -
Page 48: Dimensional Drawing - Waste Disposal Configuration [Sg403A-Wdu]
Document: 333D042B Aug-16-2007 Subject: Rev. B Dimensional Drawing – Waste Disposal Configuration [SG403A-WDU]... - Page 49 Document: 333D042B Aug-16-2007 Subject: Rev. B Dimensional Drawing – Waste Disposal Configuration [SG603A-WDU]...
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Page 50: Dimensional Drawing - Necropsy Configuration [Sg403A-Nec]
Document: 333D042B Aug-16-2007 Subject: Rev. B Dimensional Drawing – Necropsy Configuration [SG403A-NEC]... -
Page 51: Dimensional Drawing - Necropsy Configuration [Sg603A-Nec]
Document: 333D042B Aug-16-2007 Subject: Rev. B Dimensional Drawing – Necropsy Configuration [SG603A-NEC]... -
Page 52: Cable Port Illustration
Document: 333D042B Aug-16-2007 Subject: Rev. B Cable Port Illustration CABLE PORT GASKET PART# 41126 CABLE PORT RING PART# 190P728 (4) #8-32 X 5/16" WELD STUD WITH HEX CAP NUT/ LOCK WASHER / FLAT WASHER INTERIOR WALL PART#'S 10758 / 10553 / 11307 / 11279 CABLE PORT GASKET PART# 41126 REMOVABLE CABLE HOOK... - Page 53 Document: 333D042B Aug-16-2007 Subject: Rev. B Interior View Exterior View [Cable port plug not shown]...
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Page 54: Ladder Schematic: Sg403A
Document: 333D042B Aug-16-2007 Subject: Rev. B Ladder Schematic: SG403A... -
Page 55: Ladder Schematic: Sg603A
Document: 333D042B Aug-16-2007 Subject: Rev. B Ladder Schematic: SG603A... -
Page 56: Replacing Fluorescent And Ultraviolet Lamps
Document: 333D042B Aug-16-2007 Subject: Rev. B Replacing Fluorescent and Ultraviolet Lamps Fluorescent Lamp Replacement 1) Turn the fluorescent lamps off by depressing the light on/off switch locating on the right side of the light canopy. 2) Pull the vertical panel out enough at the bottom so that the thumbscrews can be accessed. -
Page 57: Ultraviolet Lamp Replacement
Document: 333D042B Aug-16-2007 Subject: Rev. B Fig. 3 4) Remove FLUOR-A-LOCK tabs (2) from the lamp sockets located on the left side of the fluorescent lamps. (Fig. 3) Using both hands, grasp the lamp carefully near each end and rotate it approx ¼ turn until the lamp is no longer seated in the lamp sockets. - Page 58 Document: 333D042B Aug-16-2007 Subject: Rev. B IMPORTANT Before replacing a UV germicidal lamp it might be necessary to check with an industrial hygienist, safety officer or other qualified person in the lab to make sure that there is nothing stored in the cabinet that could potentially be a hazard to you or could be contaminated by room air.
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Page 59: Notice - O.s.h.a. Federal Regulation
Document: 333D042B Aug-16-2007 Subject: Rev. B NOTICE – O.S.H.A. Federal Regulation The following in an excerpt form the O.S.H.A. Federal Register Page 22240 and Figure J-9. *Biological hazard signs. The biological hazard warning shall be used to signify the actual or potential presence of a biohazard and to identify equipment, containers, rooms, materials, experimental animals or combinations thereof, which contains, or are contaminated with, viable hazardous agents. -
Page 60: Warranty
The exclusive remedy for any breach or violation of this warranty is as follows: The Baker Company, Inc. will F.O.B. Sanford, Maine furnish without charge repairs to or replacement parts or equipment which proved defective in material or workmanship.
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