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Summary of Contents for Baker InvivO2 1000
- Page 1 User Manual UM-038 InvivO 1000 Dual Chamber Hypoxia Workstation InvivO 1000 Affix Serial Number Sticker Here...
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Page 2: Table Of Contents
ENVIRONMENTAL OPERATING CONDITIONS ..............6 SERVICE REQUIREMENTS ....................7 ELECTRICAL SUPPLY REQUIREMENTS ................7 GAS SUPPLY REQUIREMENTS ..................8 INVIVO2 1000 DUAL WORKSTATION OVERVIEW ..............9 FRONT VIEW ......................... 9 FRONT INTERIOR VIEWS ..................... 10 LEFT SIDE VIEW ......................11 REAR VIEW ......................... - Page 3 Unit 8&9 / York Park / Bridgend, United Kingdom / CF31 3TB +44 (0) 1656 645688 / Fax: +44 (0) 1656 667966 / sales@ruskinn.com www.bakerruskinn.com WORKSTATION EXIT ....................22 HUMIDITY CONTROL ....................23 SCREENS ........................30 MAIN SCREEN ......................30 LEFT AND RIGHT WORKSTATION MAIN MENUS ............
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Page 4: Table Of Figures
Unit 8&9 / York Park / Bridgend, United Kingdom / CF31 3TB +44 (0) 1656 645688 / Fax: +44 (0) 1656 667966 / sales@ruskinn.com www.bakerruskinn.com TABLE OF FIGURES Figure 1: Rear Connections ..................... 7 Figure 2: Gas Connection Graphic ..................8 Figure 3: InvivO Dual Workstation.................. - Page 5 Unit 8&9 / York Park / Bridgend, United Kingdom / CF31 3TB +44 (0) 1656 645688 / Fax: +44 (0) 1656 667966 / sales@ruskinn.com www.bakerruskinn.com TABLE OF SCREEN IMAGES Screen 1: Interlock Purge ....................15 Screen 2: Interlock Purge Timer ..................15 Screen 3: Workstation Selection ..................
- Page 6 Unit 8&9 / York Park / Bridgend, United Kingdom / CF31 3TB +44 (0) 1656 645688 / Fax: +44 (0) 1656 667966 / sales@ruskinn.com www.bakerruskinn.com TABLE OF TABLES Table 1: List of Symbols ....................5 Table 2: Electrical Service Requirements ................. 7 Table 3: Fuse Ratings .......................
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Page 7: Introduction
Please read this manual carefully before using the InvivO and familiarise yourself with all aspects of using the workstation. The Baker Company (Baker) or Ruskinn Technology Ltd (Ruskinn) does not accept responsibility for accidents to personnel or damage to the InvivO workstation resulting from incorrect use. -
Page 8: Safety Instructions
SAFETY INSTRUCTIONS Baker and/or Ruskinn do not take any responsibility for damages caused by using the equipment for other purposes than described in this user manual. The mains appliance coupler and plug are the AC mains supply isolation device and must be easily accessible when installed. -
Page 9: Regulatory Compliance
REGULATORY COMPLIANCE European Region This product complies with the essential EEA requirements for Electrical Safety and Electromagnetic compatibility as set out in the EMC Directive 2014/30/EU, the Low Voltage Directive 2014/35/EU and hazardous substances (RoHS) Directive 2011/65/EU has been tested and found to comply in full. For details on standards tested refer to the DoC Certificate. -
Page 10: North American Region
North American Region This product is UL 61010-1 Listed, and CSA C22.2 No. 61010-1 under file number E113911. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. -
Page 11: Symbols
Symbols Before using the InvivO , please ensure that you are familiar with the symbols. Symbol Meaning Refer to user manual. Alternating current Functional Earth Connection Protective Earth Connection This product complies with the essential EEA requirements for Electrical Safety and Electromagnetic compatibility as set out in the EMC directive 2004/108/EC and the Low Voltage Directive 2006/95/EC Caution, do not remove covers. -
Page 12: Transport And Storage
TRANSPORT AND STORAGE When not in use, the InvivO Workstation must only be stored within a temperature of between 0°C and 30°C Storage outside of this range may damage the workstation. LOCATION AND HANDLING OF THE INVIVO2 WORKSTATION The InvivO Workstation should only be installed or relocated by a qualified engineer. -
Page 13: Service Requirements
SERVICE REQUIREMENTS Electrical Supply Requirements The workstation must be connected to a mains power supply. Two power cords are supplied to connect the workstation to the mains supply. If alternative power cords are used they must be rated appropriately for the power requirements of the workstation, refer to Table 2. -
Page 14: Gas Supply Requirements
Gas Supply Requirements The workstation gas supplies are located at the rear of the unit as shown in Figure 1. For standard physiological environment operation of the workstation the gas requirements are: Regulator Output Symbol Specification Pressure 58 to 72.5psi Nitrogen Oxygen Free (4 to 5 bar) -
Page 15: Invivo2 1000 Dual Workstation Overview
10% in Nitrogen (N ) is the responsibility of the user and must be assessed by the user in accordance to local safety regulations at the installation location. INVIVO2 1000 DUAL WORKSTATION OVERVIEW Front View The InvivO Dual Workstation is shown below in Figure 3. -
Page 16: Front Interior Views
Front Interior Views Figure 4: InvivO Internal View from Left Figure 5: InvivO Internal View from Right 1. Internal Light 2. Temperature and Humidity Sensor Housing 3. Internal interlock door 4. Internal sockets 5. Humidity Outlet (Optional) 6. Condensate plate 7. -
Page 17: Left Side View
Note: For the right side of the Dual Workstation the location of all parts is a mirror image of the left Left Side View Figure 6: Left Side End Panel with Ultrasonic Humidity Option 1. Lid – Service access only 2. -
Page 18: Rear View
Rear View Figure 7: Rear End Panel and Supply Connections Figure 8: Gas and Electrical Connections 1. Ventilation Holes 2. Ethernet Port (For use with Remote Monitoring Option Only) 3. Safety label 4. Rear panel – Service access only See Service Requirements section for further details on electrical and gas connections. Page 12 of 61... -
Page 19: Using The Workstation
USING THE WORKSTATION Using the interlock The InvivO 1000 Dual workstation has a 49 litre interlock and is used for transferring materials and samples into and out of the workstation chamber and pre-conditioning the atmosphere to not affect the internal workstation atmosphere. As an optional extra (External HEPA containment package) the purge facility can be operated for both entering and exiting the workstation. -
Page 20: Operating The Interlock Inner Doors
Operating the interlock inner doors The interlock inner doors can only be opened if the interlock has been purged, indicated on the touchscreen, and the inner door push buttons being illuminated. To open the interlock inner door: Access the workstation chamber via the Ezeeyin glove ports after putting your arm through the Ezee-Sleeves. -
Page 21: Screen 1: Interlock Purge
Fast Purge 0% Purge Screen 1: Interlock Purge The interlock cycle status purge timer will appear on the touchscreen and count down the time remaining to achieve the required Oxygen level. Screen 2: Interlock Purge Timer Access the workstation chamber via the Ezeeyin glove ports after putting your arm through the Ezee-Sleeves. -
Page 22: Removing Material From The Workstation Chamber Via The Interlock
Removing material from the workstation chamber via the interlock To remove material from the workstation chamber via the interlock; Open the interlock inner door by pressing the inner door button and sliding the interlock inner door backwards. Move the material from the workstation chamber into the interlock chamber. ... -
Page 23: Hand Access To The Main Chamber
Hand access to the main chamber Direct hand access to the workstation chamber is provided via the Ezeeyin glove ports and Ezee-Sleeve The Ezee-Sleeve consists of a gloveless gas tight sleeve and a cuff. The Ezee-Sleeve attaches to the glove port via two O-rings. Note: The workstation should not be used without the Ezee-Sleeve or EzeeCuff attached. -
Page 24: Vacuum Operation
Vacuum Operation To ensure that no external atmosphere contaminates the workstation, a single vacuum operation is required before Glove Port access. To minimise the time, it is recommended to eliminate as much external atmosphere from the Ezee-Sleeve as possible prior to arm entry. -
Page 25: Figure 12: Foot Pedals For Right And Left Glove Ports
While the arm is grasping the handle, generate a vacuum by operating the foot pedal for the corresponding glove port. Figure 12: Foot pedals for right and left glove ports The vacuum operation should be continued until the maximum amount of external atmosphere has been removed from the Ezee-Sleeve and the Ezee-Sleeve exerts some pressure on the arm/hand. -
Page 26: Workstation Entry
Workstation Entry Once the vacuum has been achieved, the Glove Port Handle can now be rotated in either direction to unlock the Glove Port Cap. As there is a strong vacuum within the Ezee-Sleeve, removal of the Cap can require a reasonable amount of force. -
Page 27: Figure 17: Glove Port Cap Storage Location Inside Workstation
Figure 17: Glove port cap storage location inside workstation Repeat the procedure for the other hand (if both hands are entering the workstation chamber). Page 21 of 61... -
Page 28: Workstation Exit
Workstation Exit Remove Cap from storage brackets, and ensure the handle is oriented in a vertical position on the Cap. The Handle is designed with “indexing” detent features to help locate the Handle relative to the Cap. Drawing the handle into the Glove Port, replace the cap, using the location posts and graphic to orient the Cap correctly on the Glove Port. -
Page 29: Humidity Control
Humidity Control The InvivO workstations control the humidity of the workstation chamber from ambient to 85% relative humidity. Note: Any equipment installed in the workstation chamber must be suitable for the humidity level inside the workstation chamber. If in doubt, consult the manufacturers’ datasheet or manual for any equipment that is installed in the workstation chamber. -
Page 30: Figure 22: Humidity System Water Tank
Ultrasonic Humidity (Optional) The ultrasonic humidity option for the InvivO 1000 Dual workstation allows full control of the working humidity level in the workstation. The humidity level is controlled to ±3% of the set point to ensure that the required humidity level is maintained. The system has a large water tank as the supply for the humidity and should be filled with only deionised water to maintain the cleanliness of the system. -
Page 31: Figure 23: Water Tank Thumb Screw
The water tank is removable for cleaning, or replacement if required. To remove the water tank first unscrew the three thumb screws holding the water tank in place: Figure 23: Water Tank Thumb Screw Once these are removed the water tank can be lowered in the end panel to expose the top of the tank, then tilting the top forward will allow access to an electrical connector and a blue that need to be disconnected from the top . -
Page 32: Figure 25: Water Tank Tilted Forward Partially Removed
Figure 25: Water Tank tilted forward partially removed Figure 26: Water Tank top tube removal Page 26 of 61... -
Page 33: Figure 27: Water Tank Electrical Connector
Figure 27: Water Tank electrical connector Figure 28: Water Tank bottom lifted out Page 27 of 61... -
Page 34: Figure 29: Water Tank Bottom Tube Disconnection
Once raised out there is an additional tube to remove from the bottom of the tank. Tilt the water tank so that the level of the remaining water is below the tube connector to avoid spillage as the tube is removed. Figure 29: Water Tank bottom tube disconnection Re-installation is carried out reversing the removal procedure. -
Page 35: Figure 30: Condensate Plate
Condensate Plate If there is excess humidity in the system, or you want to run a ‘dry’ atmosphere then the condensate plate is able to reduce the humidity level. This is achieved by cooling the plate to below the dew point to allow for the humidity to condense on the plate. Figure 30: Condensate Plate As this forms droplets these are then collected into a small reservoir in the end panel that will then drain off when it fills to a pipe at the rear of the workstation. -
Page 36: Screens
SCREENS Main screen When the unit is first powered on a Ruskinn Logo screen will appear and will be replaced by Screen 3 Screen 3: Workstation Selection From this menu you will be able to access all of the features of both workstations. Selecting the Left workstation will bring up Screen 4: Left Main Menu and selecting the Right workstation will bring up Screen 5. -
Page 37: Left And Right Workstation Main Menus
Left and Right Workstation Main Menus Screen 4: Left Main Menu Screen 5: Right Main Menu The arrows on either side of the menu indicate which workstation is being controlled. Pressing the Hope icon will return to the workstation selection screen Page 31 of 61... -
Page 38: Invivo 2 Initial Settings Screen
InvivO Initial Settings Screen Pressing the “Atmosphere Control” bar on Screen 4 or Screen 5 will open up the settings screen shown in Screen 6. Screen 6: InvivO Initial Settings Screen with Ultrasonic Humidity Option Viewing the rows from left to right they display: Row 1 Row 2 Row 3... -
Page 39: Screen 8: Initial Settings Screen With Aquasorb Humidity
If the workstation does not have the ultrasonic humidity option fitted then the humidity set point is can be adjusted in increments of 5% using the arrows. The software will then allow the humidity to rise to this value and limit the maximum internal humidity. Screen 8: Initial Settings Screen with Aquasorb Humidity Page 33 of 61... -
Page 40: Environment Control
Environment Control The Environment Control screen is the main screen during use to monitor the current workstation conditions and gain access to all of the in-use functions. Screen 9: Environment Control Overview Pressing the home icon on the bottom right will bring up Screen 10 to stop gas control and return to the main menu Screen 10: Exit Gas Control Warning Page 34 of 61... -
Page 41: Workstation Overview
Workstation Overview The workstation overview screen displaying the status of both workstations can be accessed by pressing Icon 3 on either sides Screen 9. Icon 3: Workstation Overview Screen 11: Workstation Overview The environment control screens can be accessed by pressing the “Left Workstation”, or “Right Workstation”... -
Page 42: Workstation Switching
Workstation Switching Workstation Environment Control Screens Overview Figure 32 provides a quick overview of the screen flow for both sides of the workstation. Note: the Hypoxic control has only been shown for the left workstation but is available for both. Left Workstation Right Workstation Hypoxic Cycle... -
Page 43: Data Logs
Data Logs The Data Log screens can be accessed from the button on Screen 9 and will give four options of trends to view as well as the option for the safe removal of the USB memory stick. Screen 13: Trend Log Pressing the values on the graph at the top and bottom left will open up a pop-up similar to Screen 7 to allow the scale of the graph to be changed within the Min/Max limits shown at the top of the box. -
Page 44: Utilities
Utilities The Utilities Menu is accessible from Screen 9 for Gas Control. Screen 14: Utilities Menu The six buttons that control the following functions from Left to Right. Light blue backgrounds indicate the function is on, dark blue for off: Button 1, Outer Interlock Door Open. -
Page 45: Hypoxic Cycle
Hypoxic Cycle The Hypoxic Cycle menu is accessed from Screen 3 to allow for dynamic changing of the Oxygen and Carbon Dioxide levels over fixed time periods. The initial setting to for continuous cycling for up to 3 set-points. Screen 15: Hypoxic Cycle Menu - Continuous Cycle On The timing values define how long the Oxygen and Carbon Dioxide levels are maintained for once they have been achieved. -
Page 46: Screen 16: Hypoxic Cycle Menu - Continuous Cycle Off
Screen 16: Hypoxic Cycle Menu - Continuous Cycle Off If you require a finite cycle sequence then pressing the Continuous cycle button will toggle it to Off and bring up some additional settings. As with the continuous cycle you are able to select up to 3 different set-points with a time to remain at each, and if you only want 2 set-points to can set step 3 to 0 minutes and copy the set-points from step 2. -
Page 47: Screen 17: Hypoxic Cycle Temperature And Humidity Settings
Screen 17: Hypoxic Cycle Temperature and Humidity Settings Within the Hypoxic Mixing cycles the workstation will maintain the temperature and humidity settings. These settings are accessed from the button on Screen 15 or Screen 16 and operate in the same way as Screen 6.Error! Reference source not found. Page 41 of 61... -
Page 48: Oxygen Sensor Calibration
Oxygen Sensor Calibration Screen 18: Oxygen Sensor Calibration The Oxygen sensor calibration screen can be accessed from the main menu for periodic calibration. The calibration procedure uses the Nitrogen supply to determine the low (0% Oxygen) operation point and the ambient atmosphere for the high operation point. The Atmospheric Oxygen level is set to 20.9% as default but this can be changed if required. -
Page 49: Help / Settings
Help / Settings Screen 19: Help / Settings Menu In this menu you are able to adjust the time and date by pressing on the current setting and a pop-up window will appear to enter the new setting. The screen brightness can be adjusted between 0 (Dim) and 30 (Bright) in increments of 3 giving 11 brightness levels. -
Page 50: Warnings
Warnings There are various warning screens and icons to draw the user attention when required. Temperature Alarm This screen will appear if the workstation temperature is not at the set-point. This will appear during the initial turn-in or if the set-point has been changed by more than 0.2°C. The alarm can be dismissed by pressing the return arrow at the bottom right of the screen. -
Page 51: Screen 21: Water Tank Low Warning Screen
Water Level Low Alarm – Ultrasonic Humidity Only If the humidity system is turned on and the water level in the water tank falls too low then the following warning screen will appear. This can be dismissed by pressing the back arrow at the bottom right but it will appear again if the water level is still too low after 20 minutes. -
Page 52: Screen 22: Usb Memory Warning Screen
USB Alarm The USB alarm will activate if the USB memory stick has been removed for longer than 5 minutes as a reminder to replace it after the log files have been copied to a computer for analysis. This alarm can be dismissed by pressing the back arrow at the bottom right of the screen for 5 minutes and then it will re-activate. -
Page 53: Screen 23: Gas Mix Time Limit Warning Screen
Set-Point Failure Alarm Screen This alarm actives if the gas set-points have not been reached with 3 hours of mixing. This will also disable the gas control of the workstation to avoid excessive gas usage as it indicates an issue with the gas tightness of the workstation. Screen 23: Gas Mix Time Limit Warning Screen The alarm can be dismissed by pressing the back arrow at the bottom right of the screen. -
Page 54: Screen 24: Input Gas Pressure Alarm
Input Gas Pressure Alarm This screen will appear when the pressure on the required gas lines fall below the required minimum levels as defined in the Gas Supply Requirements section. Please refer to the section ‘’ for common problems and solutions Screen 24: Input Gas Pressure Alarm Alarm Purpose Alarm Buzzer Sound... -
Page 55: Usb Trend Log
USB TREND LOG The Trend Log is updated onto the USB memory stick every minute to enable to data to be downloaded and analysed as required. Note that the USB memory stick must have a FAT32 format to store the data and that the memory stick should be directly connected to the port without the use of USB extension leads. -
Page 56: Cleaning And Service Requirements
Lists the servicing requirements, intervals and persons capable of performing the service are detailed in Table 9. Important notice: Your Baker Ruskinn Workstation includes a 2 year warranty. This is only valid if the first year service is carried out in accordance with table 6 below. Action... -
Page 57: Cleaning Procedure - During And After Each Use
The correct cleaning agents must be used to clean the workstation. The use of incorrect cleaning agents will damage the workstation and invalidate the warranty. The following cleaning agents are permitted: Ethanol, laboratory grade at a maximum concentration of 70% by volume ethanol in distilled water. -
Page 58: Cleaning Procedure - Deep Clean With Front Screens Removed
Cleaning procedure – deep clean with front screens removed Preparation: Return to a main menu (Screen 4 or Screen 5), enter the settings menu Screen 19 Open the outer interlock door by pressing Icon 1: Interlock Outer Door for >3 seconds ... - Page 59 Cleaning: The components that require cleaning are; o Workstation floors. o Workstation side walls. o Workstation ceiling. o Workstation floor tray. o Interlock inner door. o Storage racks. o Front Screen. o Waste port o Interlock floor – by sliding the floor tray into the workstation. o Interlock side walls.
- Page 60 Reinstalling workstation components: Place the floor tray inside the workstation chamber. Reinstall the storage racks. Replace the waste port lid and waste port bag if fitted. Reinstall any equipment removed from the workstation chamber. Close the outer interlock doors ...
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Page 61: Year Service
1 Year Service Service Kit Contents: Large Detox Sachet Tristel Fuse Sachet with Trigger Spray Bottle Acrylic Anti-static Cleaner Sleeves Service Instructions: Follow the Deep Cleaning “Preparation” stage for both sides of the workstation. Unscrew the 2 thumb screws retaining the ceiling panel in the workstation. Figure 34: Ceiling Panel Thumb Screws Pull down the ceiling panel. -
Page 62: Figure 36: Large Detox Sachet
Remove the Large Detox Sachet and replace with the new sachet in the service kit. Figure 36: Large Detox Sachet Replace the ceiling panel and the thumbscrews. Remove the used sleeves from the front screen and dispose of them. Figure 37: Gloveport Sleeves Follow the Deep Cleaning ‘Cleaning’... -
Page 63: Troubleshooting
TROUBLESHOOTING The Workstation will not switch on Check the workstation is plugged in Check the Mains socket is switched on Check the Circuit Breaker has not tripped Check the Workstation fuses have not blown Check the plug fuses have not blown If all the above fail to switch the workstation on contact your local distributor. -
Page 64: Excessive Gas Usage
Excessive Gas Usage If you have a warning on the touchscreen for excessive gas usage as shown in Screen 23 we recommend you carry out the following to try to rectify the issue: Set the workstation to the main menu, Screen 3 ... -
Page 65: Warranty Information
WARRANTY INFORMATION Ruskinn Technology Limited warrants for the applicable time period that the InvivO will substantially perform in accordance with the user documentation. The terms of this Agreement do not affect or prejudice the statutory rights of a consumer acquiring the Ruskinn Technology Limited InvivO otherwise than in the normal course of a business. -
Page 66: Disposal Information
DISPOSAL INFORMATION InvivO contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. Alternatively, please contact your local distributor for disposal instructions. -
Page 67: Contact Details
Ruskinn Technology Limited is a registered company in United Kingdom, company number 05692599 Ruskinn Technology Limited is VAT registered in United Kingdom, VAT number 870194126 Ruskinn Technology Limited is a wholly owned subsidiary of The Baker Company Manual Version 1.1 – release date 09 November 2018 Page 61 of 61...
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