Related Manuals for Baker Ruskinn PhO2xBox
Summary of Contents for Baker Ruskinn PhO2xBox
- Page 1 User Manual UM-048 xBox Cell-Culture Chamber System xBox Affix Serial Number Sticker Here...
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Page 2: Table Of Contents
Unit 8&9 / York Park / Bridgend, United Kingdom / CF31 3TB +44 (0) 1656 645688 / Fax: +44 (0) 1656 667966 / sales@ruskinn.com www.bakerruskinn.com CONTENTS TABLE OF FIGURES ........................... I TABLE OF TABLES ............................ II INTRODUCTION ............................3 SAFETY INSTRUCTIONS ..........................4 REGULATORY COMPLIANCE ........................ - Page 3 Unit 8&9 / York Park / Bridgend, United Kingdom / CF31 3TB +44 (0) 1656 645688 / Fax: +44 (0) 1656 667966 / sales@ruskinn.com www.bakerruskinn.com Figure 10: Rear of Cell culture chamber ....................13 Figure 11: Rear of Cell culture multi chamber ..................13 Figure 12: Rear of gas controller ......................
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Page 4: Introduction
Please read this manual carefully before using the PhO xBox and familiarise yourself with all aspects of using the workstation. The Baker Company (Baker) or Ruskinn Technology Ltd (Ruskinn) does not accept responsibility for accidents to personnel or damage to the PhO xBox workstation resulting from incorrect use. -
Page 5: Safety Instructions
System. The System comprises a Gas Controller and a Cell-Culture Chamber. Baker and/or Ruskinn does not accept responsibility for accidents to personnel or damage to the Cell- Culture Chamber System resulting from incorrect use. The mains appliance coupler and plug are the AC mains supply isolation device and must be easily accessible when installed. -
Page 6: Regulatory Compliance
REGULATORY COMPLIANCE WEEE: This equipment must be disposed of in accordance with the Waste from Electrical and Electronic Equipment (WEEE) Directive This product must not be treated as household waste. Instead, it shall be handed over to an appropriate collection point for the recycling of electrical and electronic equipment. If in doubt, please return this equipment to Ruskinn Technology Ltd who will correctly dispose of it for you. -
Page 7: Symbols
Symbols Before using the PhO xBox, please ensure that you are familiar with the symbols. Symbol Meaning Refer to user manual. Alternating current Functional Earth Connection Protective Earth Connection Caution, do not remove covers. No end user serviceable parts behind covers. Please refer to this manual in all cases where this symbol appears, in order to find out the nature of the Potential Hazard and actions to be taken in order to avoid the Hazard. -
Page 8: Transport And Storage
TRANSPORT AND STORAGE When not in use, the PhO xBox controller box & cell culture chamber must only be stored within a temperature of between 0°C and 30°C Storage outside of this range may damage the gas controller. LOCATION AND HANDLING OF THE PHO XBOX The PhO xBox should only be installed or relocated by a qualified engineer. -
Page 9: Service Requirements
SERVICE REQUIREMENTS Electrical Supply Requirements The Gas Controller must be connected to a mains power supply. A power cord is supplied to connect the workstation to the mains supply. If an alternative power cord is used is must be rated appropriately for the power requirements of the Gas Controller, refer to Table 2. -
Page 10: Pho 2 Xbox Overview
XBOX OVERVIEW Background The System comprises a Cell Culture Chamber , which comes in two sizes (Small and Large) and different colours (Black & Transparent), and a Gas Controller. The Gas Controller is connected to the Cell Culture Chamber. The Cell Culture Chamber can hold cell culture flasks and 6, 12, 24 or 96 well plates. -
Page 11: Figure 1: Blue Tube
Figure 1: Blue Tube Figure 2: Mains Lead Figure 3: Signal Lead Figure 4: Gas Controller (With air pump) -
Page 12: Figure 5: N 2 Gas Hose
Figure 5: N Gas Hose Figure 6: CO Gas Hose Figure 7: Cell Culture Chamber (Small QTK-A01) Figure 8: Cell Culture Chamber (Large QTK-A02) -
Page 13: Figure 9: Cell Culture Chamber (Large Qtk-Ab02)
Figure 9: Cell Culture Chamber (Large QTK-AB02) -
Page 14: Setting Up The Gas Controller And Culture Chamber
SETTING UP THE GAS CONTROLLER AND CULTURE CHAMBER Setting up the cell culture chamber on a laboratory bench The Cell Culture Chamber can be placed adjacent to or on top of the Gas Controller. Connect the blue tube to InA on the Cell Culture Chamber and OutA on the Gas Controller. Connect the signal lead to InB on the Cell Culture Chamber and OutB on the Gas Controller. -
Page 15: For Installation Inside A Workstation (Sci-Tive Or Invivo 2 ) Or Co 2 Incubator
Figure 12: Rear of gas controller Use the blue hose to connect N on the Gas Controller to the N gas cylinder and the black hose to connect CO on the Gas Controller to the CO cylinder. Connect the mains lead to the right port shown on Gas Controller. Open gas cylinders and turn the gas controller power on. -
Page 16: Figure 14: Inside View Of Invivo
Figure 14: Inside view of InvivO showing Universal Multi-Cable Gland Figure 15: Black frame and blue plug Unscrew inside black frame of the Universal Multi-Cable Gland and remove the blue plug. The Plug can contain up to 6 pre-drilled holes depending on how it was ordered. Additional holes can also be drilled by user. -
Page 17: Figure 16: Multi-Cable Gland
Figure 16: Multi-cable Gland Use the blue hose to connect N on the Gas Controller to the N gas cylinder and the black hose to connect CO on the Gas Controller to the CO cylinder. Connect the mains lead to the right port shown on Gas Controller. Figure 17: Pneumatic schematic Open gas cylinders and turn the Gas Controller power on. -
Page 18: Using The Gas Controller
USING THE GAS CONTROLLER Gas Controller The Gas Controller is shown as follows. Figure 18: Front View Figure 19: Left View Figure 20: Right View Figure 21: Rear View... -
Page 19: Figure 22: Rear View (Multi Version)
Figure 22: Rear View (Multi version) Figure 23: Top View... -
Page 20: The Touch Screens
THE TOUCH SCREENS Start up screens When the unit is first powered a Logo Screen (Screen 1) will appear and then will be replaced by the Main Screen (Screen 2). Figure 24: Screen 1 – Logo screen Main screens From this menu you will be able to access the Main screen below. Figure 25: Screen 2 –... -
Page 21: Atmosphere Control Screen
Figure 26: Multi chamber version only – Chamber selection screen Once chamber has been selected the main menu is then displayed with the currently active chamber displayed in the top left corner. Figure 27: Multi chamber version main screen Atmosphere Control Screen Pressing the “Atmosphere Control”... -
Page 22: Figure 27: Screen 4 - Level Set Point Screen
O₂ and CO₂ set point can be changed by pressing O2 SET POINT or CO2 SET POINT. A pop-up will appear for the new setting to be selected, as shown in Screen 4. Press Enter once the desired value of O is selected. -
Page 23: Re-Setting The Oand Co Value During Operation
Re-setting the O and CO value during operation To re-set the value during operation, press the “stop” button, change the value, and press the “start” button. The new value will now become the Set Point. Figure 30: Screen 5 – Atmosphere Control Screen Hypoxic Cycle Menu Settings The Hypoxic Cycle menu is accessed from Screen 2 to allow for dynamic changing of the Oxygen and Carbon Dioxide levels over fixed time periods. -
Page 24: Figure 30: Screen 7 - Trend Log
Data Trend Log The Data Trend Log screen can be accessed from the LOG button on screen 6. Screen 7 shows the Trend Log display. The earliest recorded data will be displayed. The window can be scrolled through on the control screen. To view previous data, the USB is to be removed and connected to a computer. - Page 25 Parameter Ranges and Meanings Offset: range is 0.1-1.0%, when the set point and current value difference is less than offset the unit enters the intermittent adjustment mode. Deadband setting: range is 0-0.5%, for example when the deadband setting is 0.2% and O set point is 5.0%, when the current value is 4.8%-5.2%, no gases will be injected to adjust the concentration.
- Page 26 The Cell Culture Chamber is connected to Gas Controller, which contains O₂ and CO₂ sensors. A dish of distilled water can be installed in the culture chamber to increase humidity Cleaning: Use special cleaning kit supplied by Baker Ruskinn (code 262-345) or < 70% ethanol wiping, please do not use ultraviolet irradiation.
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Page 27: Warranty Information
WARRANTY INFORMATION Ruskinn Technology Limited warrants for the applicable time period that the PhO xBox will substantially perform in accordance with the user documentation. The terms of this Agreement do not affect or prejudice the statutory rights of a consumer acquiring the Ruskinn Technology Limited xBox otherwise than in the normal course of a business. -
Page 28: Disposal Information
DISPOSAL INFORMATION xBox contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. Alternatively, please contact your local distributor for disposal instructions. xBox contains recyclable parts. -
Page 29: Contact Details
Ruskinn Technology Limited is a registered company in United Kingdom, company number 05692599 Ruskinn Technology Limited is VAT registered in United Kingdom, VAT number 870194126 Ruskinn Technology Limited is a wholly owned subsidiary of The Baker Company Manual Version 1.0 – release date 28 Jun 2019...
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