Page 1 Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 2: Warranty
Copyright adhered to, could result in damage manual and any information contained herein, including but not limited to the © Agilent Technologies, Inc. to the product or loss of important implied warranties of merchantability 2014-2024 data. Do not proceed beyond a and fitness for a particular purpose.
Page 3: Table Of Contents
Contents In This Book 7 Introduction 8 Overview of the Module 9 Product Description of the 1290 Infinity III Diode Array Detector FS (G7117A) 10 Features of the 1290 Infinity III Diode Array Detector FS (G7117A) 11 Product Description of the 1290 Infinity III Diode Array Detector (G7117B) 12 Features of the 1290 Infinity III Diode Array Detector (G7117B) 13 Product Description of the 1260 Infinity III Diode Array Detector HS (G7117C) 14...
Page 4 Using the Module 56 General Information 57 Preparation of the System 61 Preparing the Module 71 Optimizing the Performance of the Module 78 Introduction 79 Optimization Overview 80 Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 86 Optimizing Selectivity 96 Optimizing the Detector Regarding to the System 98 Warm up of the Detector 104 Diagnostics and Troubleshooting 106 Diagnostic Features 107 Overview of Available Tests and Tools 108...
Page 5 Correcting Leaks 191 Replace Leak Handling System Parts 193 Replace the Module Firmware 196 Information from Module’s Assemblies 198 Parts and Materials for Maintenance 199 Overview of Maintenance Parts 200 Accessory Kit (G7117-68755) 202 Identifying Cables 203 Cable Overview 204 Analog Cables 206 Remote Cables 208 BCD Cables 212 CAN/LAN Cables 214 RS-232 Cables 215 USB 216 Hardware Information 217 General Hardware Information 218...
Page 6 Waste Electrical and Electronic Equipment (WEEE) Directive 271 Radio Interference 272 RFID Statement 273 Sound Emission 275 Agilent Technologies on Internet 276 Diode Array Detectors User Manual...
Page 7: In This Book
9007202778604299 ag-textnode-base-id: 3523863307 In This Book This manual covers the following Agilent InfinityLab LC Series modules: • Agilent 1290 Infinity III Diode Array Detector FS (with fixed slit) (G7117A) • Agilent 1290 Infinity III Diode Array Detector (with variable slit) (G7117B) •...
Page 8: Introduction
ag-textnode-version-id: 18014402491637387 ag-textnode-base-id: 3982155403 Introduction This chapter gives an introduction to the module and instrument overview. Overview of the Module 9 Product Description of the 1290 Infinity III Diode Array Detector FS (G7117A) 10 Features of the 1290 Infinity III Diode Array Detector FS (G7117A) 11 Product Description of the 1290 Infinity III Diode Array Detector (G7117B) 12 Features of the 1290 Infinity III Diode Array Detector (G7117B) 13 Product Description of the 1260 Infinity III Diode Array Detector HS...
Page 9: Overview Of The Module
Introduction Overview of the Module ag-textnode-version-id: 9007202781196683 ag-textnode-base-id: 3526455691 Overview of the Module The detector described in this manual is designed for highest optical performance, GLP compliance and easy maintenance. It includes the following features: • Maximum of 120 Hz (G7117A/G7117C) or 240 Hz (G7117B) data acquisition rate •...
Page 10: Product Description Of The 1290 Infinity Iii Diode Array Detector Fs (G7117A)
Introduction Product Description of the 1290 Infinity III Diode Array Detector FS (G7117A) ag-textnode-version-id: 9007202781233035 ag-textnode-base-id: 3526492043 Product Description of the 1290 Infinity III Diode Array Detector FS (G7117A) The Agilent 1290 Infinity III Diode Array Detector FS (with fixed slit) is based on the Agilent Max-Light cartridge cell with optofluidic waveguides that improve light transmission to near 100% efficiency without sacrificing resolution caused by cell dispersions effects.
Page 11: Features Of The 1290 Infinity Iii Diode Array Detector Fs (G7117A)
Introduction Features of the 1290 Infinity III Diode Array Detector FS (G7117A) ag-textnode-version-id: 9007204956699019 ag-textnode-base-id: 5701958027 Features of the 1290 Infinity III Diode Array Detector FS (G7117A) • Universal Agilent Max-Light standard cartridge cell with 10 mm optical path length provides high sensitivity (noise: < ± 3 µAU) and low peak dispersion for 2.1 , 3 and 4.6 mm id columns.
Page 12: Product Description Of The 1290 Infinity Iii Diode Array Detector (G7117B)
Introduction Product Description of the 1290 Infinity III Diode Array Detector (G7117B) ag-textnode-version-id: 9007202781237515 ag-textnode-base-id: 3526496523 Product Description of the 1290 Infinity III Diode Array Detector (G7117B) The Agilent 1290 Infinity III Diode Array Detector (with variable slit) is based on the Agilent Max-Light cartridge cell with optofluidic waveguides that improve light transmission to near 100% efficiency without sacrificing resolution caused by cell dispersions effects.
Page 13: Features Of The 1290 Infinity Iii Diode Array Detector (G7117B)
Introduction Features of the 1290 Infinity III Diode Array Detector (G7117B) ag-textnode-version-id: 9007204956704011 ag-textnode-base-id: 5701963019 Features of the 1290 Infinity III Diode Array Detector (G7117B) • Universal Agilent Max-Light standard cartridge cell with 10 mm optical path length provides high sensitivity (noise: < ± 3 µAU) and low peak dispersion for 2.1 , 3 and 4.6 mm id columns.
Page 14: Product Description Of The 1260 Infinity Iii Diode Array Detector Hs (G7117C)
Introduction Product Description of the 1260 Infinity III Diode Array Detector HS (G7117C) ag-textnode-version-id: 9007203786128523 ag-textnode-base-id: 4531387531 Product Description of the 1260 Infinity III Diode Array Detector HS (G7117C) The Agilent 1260 Infinity III Diode Array Detector HS (with fixed slit) is based on the Agilent Max-Light cartridge cell with optofluidic waveguides that improve light transmission to near 100 % efficiency without sacrificing resolution caused by cell dispersions effects.
Page 15: Features Of The 1260 Infinity Iii Diode Array Detector Hs (G7117C)
Introduction Features of the 1260 Infinity III Diode Array Detector HS (G7117C) ag-textnode-version-id: 5701977995 ag-textnode-base-id: 5701977995 Features of the 1260 Infinity III Diode Array Detector HS (G7117C) • Ultra sensitivity through revolutionary Agilent Max-Light cartridge cell with 60 mm optical path length (typically noise: <± 0.6 µAU) •...
Page 16: Operating Principle
Introduction Operating Principle ag-textnode-version-id: 3982189579 ag-textnode-base-id: 3982189579 Operating Principle ag-textnode-version-id: 3526460171 ag-textnode-base-id: 3526460171 Optical System The optical system of the detector is shown in Figure 4 on page 16. Figure 4: Optical System of the Detector UV-lamp Lamp mirror Flow cell Fold mirror Programmable (G7117B) or Fixed (G7117A/G7117C) slit Grating Array...
Page 17 Introduction Operating Principle ag-textnode-version-id: 3526464651 ag-textnode-base-id: 3526464651 Lamp The light source for the UV-wavelength range is a long-life UV-lamp with RFID tag. As a result of plasma discharge in low-pressure deuterium gas, the lamp emits light over the 190 nm to approximately 800 nm wavelength range. Figure 5: UV-Lamp ag-textnode-version-id: 3526473611 ag-textnode-base-id: 3526473611...
Page 18 Introduction Operating Principle The optical principle of the Max-Light Cartridge cell is based on opto-fluidic waveguides. Nearly 100 % light transmission is achieved by utilizing total internal reflection in a non-coated silica fiber. Compromising refractive index and thermal effects are almost completely eliminated, resulting in significantly less baseline drift.
Page 19 Introduction Operating Principle Figure 7: Slit Assembly The slit width influences the spectral resolution and noise. Figure 8: Influence of slitwidth on resolution and noise level ag-textnode-version-id: 3526482571 ag-textnode-base-id: 3526482571 Fixed Slit (G7117A/G7117C) The fixed slit combines the required optical functions - slit and shutter - in a simple and compact component.
Page 20 Introduction Operating Principle ag-textnode-version-id: 3526469131 ag-textnode-base-id: 3526469131 Grating and Diode Array The combination of dispersion and spectral imaging is accomplished by using a concave holographic grating. The grating separates the light beam into all its component wavelengths and reflects the light onto the photodiode array. The diode array is a series of 1024 ...
Page 21: Hydraulic Path
Introduction Operating Principle ag-textnode-version-id: 3526505483 ag-textnode-base-id: 3526505483 Hydraulic Path Flow cell cartridge Column Waste Figure 10: Hydraulic path Diode Array Detectors User Manual...
Page 22: Site Requirements And Specifications
ag-textnode-version-id: 18014402492269451 ag-textnode-base-id: 3982787467 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications. Site Requirements 23 Power Consideration 23 Power Cords 24 Bench space 25 Environment 25 Specifications of the 1290 Infinity III Diode Array Detector FS (G7117A) 27 Specifications of the 1290 Infinity III Diode Array Detector (G7117B) 30 Specifications of the 1260 Infinity III Diode Array Detector HS (G7117C) 33 Specification Conditions 36 Time constant versus response time 36...
Page 23: Site Requirements
Site Requirements and Specifications Site Requirements ag-textnode-version-id: 3524769675 ag-textnode-base-id: 3524769675 Site Requirements A suitable environment is important to ensure optimal performance of the module. ag-textnode-version-id: 3535537419 ag-textnode-base-id: 3535537419 Power Consideration The module power supply has wide ranging capabilities and accepts any line voltage in the range mentioned in Specifications of the 1290 Infinity III Diode Array Detector FS (G7117A)
Page 24: Power Cords
— Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. — Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Page 25: Bench Space
Site Requirements and Specifications Site Requirements Electrical shock hazard WARNING ag-struc-frag-safety-version-id: 3003281291 ag-struc-frag-safety-base-id: 3003281291 Solvents may damage electrical cables. — Prevent electrical cables from getting in contact with solvents. — Exchange electrical cables after contact with solvents. ag-textnode-version-id: 3549515915 ag-textnode-base-id: 3549515915 Bench space The module dimensions and weight (see Physical Specifications) allow you to place the module on almost any desk or laboratory bench.
Page 26 Site Requirements and Specifications Site Requirements Condensation within the module CAUTION ag-struc-frag-safety-version-id: 3003284747 ag-struc-frag-safety-base-id: 3003284747 Condensation can damage the system electronics. — Do not store, ship or use your module under conditions where temperature fluctuations could cause condensation within the module. —...
Page 27: Specifications Of The 1290 Infinity Iii Diode Array Detector Fs (G7117A)
Site Requirements and Specifications Specifications of the 1290 Infinity III Diode Array Detector FS (G7117A) ag-textnode-version-id: 18014404180300555 ag-textnode-base-id: 5670818571 Specifications of the 1290 Infinity III Diode Array Detector FS (G7117A) Table 1: Physical specifications of the 1290 Infinity III Diode Array Detector FS (G7117A) Type Specification Comments...
Page 28 Site Requirements and Specifications Specifications of the 1290 Infinity III Diode Array Detector FS (G7117A) Type Specification Short term signal With 10 mm Max-Light cartridge cell: <±3·10 AU/cm at 230 nm, slit width noise (ASTM) 4 nm, TC 2 s With 60 mm Max-Light cartridge cell: <±0.6·10 AU/cm at 230 nm, slit width 4 nm, TC 2 s Drift...
Page 29 Site Requirements and Specifications Specifications of the 1290 Infinity III Diode Array Detector FS (G7117A) Type Specification RFID for electronics records of flow cell and UV lamp conditions (path length, volume, product number, serial number, test passed, usage) Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user settable limits and feedback messages.
Page 30: Specifications Of The 1290 Infinity Iii Diode Array Detector (G7117B)
Site Requirements and Specifications Specifications of the 1290 Infinity III Diode Array Detector (G7117B) ag-textnode-version-id: 18014404180302731 ag-textnode-base-id: 5670820747 Specifications of the 1290 Infinity III Diode Array Detector (G7117B) Table 3: Physical specifications of the 1290 Infinity III Diode Array Detector (G7117B) Type Specification Comments Weight 11.5 kg (25.4 lbs) Dimensions (height ×...
Page 31 Site Requirements and Specifications Specifications of the 1290 Infinity III Diode Array Detector (G7117B) Type Specification Short term signal With 10 mm Max-Light cartridge cell: <±3·10 AU/cm at 230 nm, slit width noise (ASTM) 4 nm, TC 2 s With 60 mm Max-Light cartridge cell: <±0.6·10 AU/cm at 230 nm, slit width 4 nm, TC 2 s Drift...
Page 32 Site Requirements and Specifications Specifications of the 1290 Infinity III Diode Array Detector (G7117B) Type Specification Communication LAN, Controller Area Network (CAN), USB ERI: ready, start, stop and shut-down signals RFID for electronics records of flow cell and UV lamp conditions (path length, volume, product number, serial number, test passed, usage).
Page 33: Specifications Of The 1260 Infinity Iii Diode Array Detector Hs (G7117C)
Site Requirements and Specifications Specifications of the 1260 Infinity III Diode Array Detector HS (G7117C) ag-textnode-version-id: 18014404180304907 ag-textnode-base-id: 5670822923 Specifications of the 1260 Infinity III Diode Array Detector HS (G7117C) Table 5: Physical specifications of the 1260 Infinity III Diode Array Detector HS (G7117C) Type Specification Comments...
Page 34 Site Requirements and Specifications Specifications of the 1260 Infinity III Diode Array Detector HS (G7117C) Type Specification Short term signal With 10 mm Max-Light cartridge cell: <±3·10 AU/cm at 230 nm, slit width noise (ASTM) 4 nm, TC 2 s With 60 mm Max-Light cartridge cell: <±0.6·10 AU/cm at 230 nm, slit width 4 nm, TC 2 s Drift...
Page 35 Site Requirements and Specifications Specifications of the 1260 Infinity III Diode Array Detector HS (G7117C) Type Specification RFID for electronics records of flow cell and UV lamp conditions (path length, volume, product number, serial number, test passed, usage). Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user settable limits and feedback messages.
Page 36: Specification Conditions
Site Requirements and Specifications Specification Conditions ag-textnode-version-id: 3526787595 ag-textnode-base-id: 3526787595 Specification Conditions Following many of the principles outlined in ASTM method E165798. Reference conditions: • Wavelength: 230 nm/4 nm with Reference Wavelength 360 nm/100 nm, Slitwidth 4 nm, TC 2 s, (or with RT = 2.2 * TC), ASTM •...
Page 37: Installation
ag-textnode-version-id: 9007204926368011 ag-textnode-base-id: 5671627019 Installation The installation of the module will be done by an Agilent service representative. In this chapter, only installation of user-installable options and accessories are described. Installing Capillaries 38 Install Capillaries 38 Handling Leak and Waste 43 Drain Connectors Installation 46 Waste Concept 51 Waste Guidance 51 Leak Sensor 52...
Page 38: Installing Capillaries
Installation Installing Capillaries ag-textnode-version-id: 45036000351652875 ag-textnode-base-id: 4077947915 Installing Capillaries This section provides information on how to install capillaries and fittings. Diode Array Detectors User Manual...
Page 39: Install Capillaries
Installation Installing Capillaries ag-textnode-version-id: 9007202257747211 ag-textnode-base-id: 3003006219 Install Capillaries Capillaries and connections depend on which system is installed. As you move to smaller-volume, high-efficiency columns, you will want to use NOTE narrow id tubing, as opposed to the wider id tubing used for conventional HPLC instruments.
Page 40 Installation Installing Capillaries From 5500-1217 (Capillary, ST, 0.17 mm x 900 mm SI/SX) Pump Vialsampler with ICC 5500-1157 (Capillary ST 0.12 mm x 500 mm SL/S) Multisampler MCT Valve/Heat Exchanger 5500-1251 (Capillary ST 0.12 mm x 400 mm SL/SL) Vialsampler MCT Valve/Heat Exchanger 5500-1238 (Capillary ST 0.12 mm x 105 mm SL/SL) Vialsampler ICC Heat Exchanger 5500-1249 (Capillary ST 0.12 mm x 120 mm SL/SL, long...
Page 41 Installation Installing Capillaries From 5500-1419 (Capillary MP35N 0.17 mm x 500 mm, SI/SI) Pump Multisampler 5500-1279 (Capillary MP35N 0.12 mm x 500 mm SI/SI) Multisampler 5500-1578 (Quick Connect Capillary MP35N 0.12 mm x MCT Heat Exchanger Column 105 mm) 5500-1596 (Quick Turn Capillary MP35N 0.12 mm x 280 mm) Column/MCT Valve Detector (DAD) 5500-1598 (Quick Turn Capillary MP35N 0.12 mm x 500 mm) Column/MCT Valve Detector (VWD)
Page 42 Installation Installing Capillaries 3 Carefully slide the ferrule components on after the nut and then finger-tighten the assembly while ensuring that the tubing is completely seated in the bottom of the end fitting. 4 Use a stable port installed to the module to gently tighten the fitting facing to the module.
Page 43: Handling Leak And Waste
36028800545451531 ag-textnode-base-id: 3526487563 Handling Leak and Waste The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L.
Page 44 Installation Handling Leak and Waste Figure 11: Infinity III Leak Waste Concept (Flex Bench installation) Diode Array Detectors User Manual...
Page 45 Installation Handling Leak and Waste Figure 12: Infinity III Single Stack Leak Waste Concept (bench installation) Diode Array Detectors User Manual...
Page 46 Installation Handling Leak and Waste Figure 13: Infinity III Two Stack Leak Waste Concept (bench installation) The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container. Diode Array Detectors User Manual...
Page 47: Drain Connectors Installation
Installation Handling Leak and Waste ag-textnode-version-id: 9007209510841867 ag-textnode-base-id: 10256100875 Drain Connectors Installation Drain Connectors have been developed to improve leak drainage for low flow leaks of high viscosity solvents (for example, isopropanol) in Agilent InfinityLab LC Series Systems. Install these parts to modules where they are missing (usually preinstalled).
Page 48 Installation Handling Leak and Waste Table 11: Compatibility of drain connectors and modules Drain Connector Type Compatible Module Compatible Module Type Double G7116A/B Column Compartment Single G7114A/B Detector G7115A G7117A/B/C G7121A/B G7162A/B G7165A G7129A/B/C Sampler G7167A/B/C G5668A G7137A G7157A G4767A G7122A Degasser G7104A/C Pump...
Page 49 Installation Handling Leak and Waste In case of incorrect installation, drain connectors cannot fully perform the NOTE intended function. It is not required to power off the HPLC stack to install Single and Double Drain NOTE Connectors. The installation of the connectors does not affect the analysis performed during the installation.
Page 50 Installation Handling Leak and Waste 1 Align the ring with the leak drain outlet of the module, press slightly with the fingers, and slide the connector along the leak drain outlet until it is aligned with the front of the leak drain. Make sure that the following requirements are covered: •...
Page 51: Waste Concept
Installation Handling Leak and Waste ag-textnode-version-id: 9007202332686987 ag-textnode-base-id: 3077945995 Waste Concept Agilent recommends using the 5043-1221 (6 L waste can with 1 Stay Safe cap GL45 with 4 ports) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. ag-textnode-version-id: 3596422027 ag-textnode-base-id: 3596422027 Waste Guidance...
Page 52: Leak Sensor
Installation Handling Leak and Waste The waste drainage must go straight into the waste containers. The waste flow NOTE must not be restricted at bends or joints. ag-textnode-version-id: 3002300811 ag-textnode-base-id: 3002300811 Leak Sensor Solvent incompatibility CAUTION ag-struc-frag-safety-version-id: 3004406283 ag-struc-frag-safety-base-id: 3004406283 The solvent DMF (dimethylformamide) leads to corrosion of the leak sensor.
Page 53: Connecting Modules And Control Software
WARNING ag-struc-frag-safety-version-id: 3822076043-1 ag-struc-frag-safety-base-id: 3822076043 Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury. — Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Page 54: Instrument Configuration
Installation Instrument Configuration ag-textnode-version-id: 3283594379 ag-textnode-base-id: 3283594379 Instrument Configuration Example shows an instrument configuration with a Diode Array Detector. 1 Set the switches of the Configuration switch at the rear of the module: a All switches DOWN: module uses the default IP address 192.168.254.11. b Switch 4 UP and others DOWN: module uses DHCP.
Page 55 Installation Instrument Configuration b Lab Advisor (Instrument Overview - Add Instrument): Diode Array Detectors User Manual...
Page 56: Using The Module
ag-textnode-version-id: 18014404181121291 ag-textnode-base-id: 5671639307 Using the Module This chapter provides information on how to use the module. General Information 57 Turn On/Off 57 Status Indicators 59 Preparation of the System 61 Prepare a Run 61 Prime and Purge the System 68 Preparing the Detector 70 Preparing the Module 71 The Detector User Interface 71 Detector Control Settings 73 Method Parameter Settings 74...
Page 57: General Information
Using the Module General Information ag-textnode-version-id: 36028801094302731 ag-textnode-base-id: 4075338763 General Information ag-textnode-version-id: 18014401511325195 ag-textnode-base-id: 3001843211 Turn On/Off This procedure exemplarily shows an arbitrary LC stack configuration. Diode Array Detectors User Manual...
Page 58 Using the Module General Information 2 On/Off switch: On 3 Turn instrument On/Off with the control software. Diode Array Detectors User Manual...
Page 59: Status Indicators
Using the Module General Information 4 On/Off switch: Off ag-textnode-version-id: 36028800065377547 ag-textnode-base-id: 3046413579 Status Indicators The module status indicator indicates one of six possible module conditions. Diode Array Detectors User Manual...
Page 60 Using the Module General Information Figure 15: Arbitrary LC stack configuration (example) Idle Run mode Not-ready. Waiting for a specific pre-run condition to be reached or completed. Error mode - interrupts the analysis and requires attention (for example, a leak or defective internal components).
Page 61: Preparation Of The System
Using the Module Preparation of the System ag-textnode-version-id: 63050398769392779 ag-textnode-base-id: 3986205835 Preparation of the System ag-textnode-version-id: 18014403027169547 ag-textnode-base-id: 4517687563 Prepare a Run This procedure exemplarily shows how to prepare a run. Parameters as shown in the screenshots may vary, depending on the system installed. Toxic, flammable and hazardous solvents, samples and reagents WARNING ag-struc-frag-safety-version-id: 3004430475-1...
Page 62 Using the Module Preparation of the System 1 Switch on the detector. 2 Fill the solvent bottles with adequate solvents for your application. 3 Place solvent tubings with bottle head assemblies into the solvent bottles. 4 Place solvent bottles into the solvent cabinet. Diode Array Detectors User Manual...
Page 63 Using the Module Preparation of the System 5 Solvent bottle filling dialog (in the software). Diode Array Detectors User Manual...
Page 64 Using the Module Preparation of the System 6 Purge the pump. For details on priming and purging, refer to the technical note Best Practices for NOTE Using an Agilent LC System Technical Note (InfinityLab-BestPractice-en- SD-29000194.pdf, SD-29000194). 7 Change solvent type if necessary. Diode Array Detectors User Manual...
Page 65 Using the Module Preparation of the System 8 Choose the tray format of the sampler. Diode Array Detectors User Manual...
Page 66 Using the Module Preparation of the System 9 Add a new column. 10 Enter the column information. Diode Array Detectors User Manual...
Page 67 Using the Module Preparation of the System 11 Select the column in the Method settings of the column compartment. 12 Set the detector parameters according to the needs of your method. Diode Array Detectors User Manual...
Page 68 Using the Module Preparation of the System Diode Array Detectors User Manual...
Page 69: Prime And Purge The System
Using the Module Preparation of the System ag-textnode-version-id: 9007202538343307 ag-textnode-base-id: 3283602315 Prime and Purge the System When the solvents have been exchanged or the pumping system has been turned off for a certain time (for example, overnight) oxygen will re-diffuse into the solvent channel between the solvent reservoir, vacuum degasser (when available in the system) and the pump.
Page 70: Preparing The Detector
Using the Module Preparation of the System ag-textnode-version-id: 3526518923 ag-textnode-base-id: 3526518923 Preparing the Detector For best performance of the detector • Let the lamp warm-up and stabilize for at least one hour (initial turn on of the module requires a longer time depending on the environment and the application needs);...
Page 71: Preparing The Module
Using the Module Preparing the Module ag-textnode-version-id: 18014402495146251 ag-textnode-base-id: 3985664267 Preparing the Module ag-textnode-version-id: 9007202781697419 ag-textnode-base-id: 3526956427 The Detector User Interface Within the detector GUI, there are active areas. If you move the mouse cursor across the icons the cursor will change. 1.
Page 72 Using the Module Preparing the Module EMF Status shows Run / Ready / Error state and “Not Ready text” or “Error text” • Offline (gray) • Ok. No Maintenance required (green) • EMF warning. Maintenance might be required (yellow) • EMF warning. Maintenance required (red) Important: The EMF settings can be accessed via Agilent Lab Advisor.
Page 73: Detector Control Settings
Using the Module Preparing the Module ag-textnode-version-id: 3526960907 ag-textnode-base-id: 3526960907 Detector Control Settings The figure shows the default settings. • Lamps: can be turned ON/OFF. • Analog Output Range: can be set to either 100 mV or 1 Vfull scale, for additional settings see Analog Output (under Method Parameter Settings...
Page 74: Method Parameter Settings
Using the Module Preparing the Module ag-textnode-version-id: 9007202781706379 ag-textnode-base-id: 3526965387 Method Parameter Settings These settings are available via Menu > Instrument > Set up Instrument Method or via right click into the module’s active area (does not show the Instrument Curves tab). Figure 16: Method parameter settings For additional help and support: Highlight the desired cell and press F1.
Page 75 Using the Module Preparing the Module Table 13: Method Parameter Settings Signals Up to 8 individual signals can be set. For each of the signals, the wavelength and bandwidth can be set for sample and reference. Limits: • Wavelength: 190.0 to 640.0 nm in steps of 0.1 nm •...
Page 76 Using the Module Preparing the Module Spectrum Settings • None • All • Range (from - to) • Step width Analog Output The range can be set to either 100 mV or 1 V full scale, see Detector Control Settings on page 73. •...
Page 77 Using the Module Preparing the Module Timetable You may set up time events to change functions with their parameters over the run time. Add lines as required. Time Limits: 0.00 to 99999.00 min in steps of 0.01 min. Via the buttons in the bottom area, time table lines can be added, removed, cut copied, pasted or completely cleared.
Page 78: Optimizing The Performance Of The Module
ag-textnode-version-id: 18014402569601291 ag-textnode-base-id: 4060119307 Optimizing the Performance of the Module This chapter provides information on how to optimize the module. Introduction 79 Optimization Overview 80 Choosing a Flow Cell 81 Inline Pressure Relief Valve Kit (G4212-68001) 84 Application Information 84 Special Information of 60 mm Cartridge Flow Cell 84 Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 86 Flow Cell Path Length 86 Peak Width (Response Time) 87...
Page 79: Introduction
Optimizing the Performance of the Module Introduction ag-textnode-version-id: 3358154891 ag-textnode-base-id: 3358154891 Introduction The detector has a variety of parameters that can be used to optimize performance. Depending on whether signal or spectral data need to be optimized, different settings are recommended. The following sections describe optimization for: •...
Page 80: Optimization Overview
Optimizing the Performance of the Module Optimization Overview ag-textnode-version-id: 3523971083 ag-textnode-base-id: 3523971083 Optimization Overview Table 14: Optimization overview Parameter Impact 1 Selection of flow cell • peak resolution versus sensitivity • Choose flow cell according to used column (Choosing a Flow Cell page 81).
Page 81: Choosing A Flow Cell
Optimizing the Performance of the Module Optimization Overview Parameter Impact • Use 4 nm slit for normal applications. • spectral resolution, sensitivity and • Use narrow slit (e.g 1 nm) if your analytes have narrow absorbance bands linearity. and for high concentrations. •...
Page 82: High Sensitivity
Optimizing the Performance of the Module Optimization Overview High Sensitivity If higher sensitivity is necessary, the G4212-60007 (Max-Light Cartridge Cell (60 mm, V(σ) 4.0 μL)) can be used. This cell enhances the detector by lowering the limit of detection (LOD) by a factor of about 3 (depending on the application). Normal Applications The G4212-60008 (Max-Light Cartridge Cell (10 mm, V(σ) 1.0 μL)) covers a wide range of applications:...
Page 83 Optimizing the Performance of the Module Optimization Overview ag-textnode-version-id: 3526203275 ag-textnode-base-id: 3526203275 Light-Sensitive Samples (LSS) Aperture (G7117-60101) for Max-Light Cartridge Cell LSS 10 mm To cartridge To detector Figure 17: Aperture G7117-60101 • The Aperture should be installed for light-sensitive samples, which are likely to undergo photodegradation.
Page 84: Inline Pressure Relief Valve Kit (G4212-68001)
The pressure relief valve is designed to protect the Max-Light Cartridge Flow Cell. Agilent Technologies strongly recommend installing the pressure relief valve at the outlet of the detector as soon as a second detector is installed like in LC/MS applications.
Page 85 Optimizing the Performance of the Module Optimization Overview Due to the larger dispersion volume, the 60 mm cell is primarily designed for 4.6 mm column applications to achieve highest sensitivity with no additional peak broadening. However, if sensitivity is important the 60 mm cell will also be advantageous in case of smaller columns (3 mm, 2.1 mm) but depending on the chromatographic system and method additional peak broadening might occur.
Page 86: Optimizing For Sensitivity, Selectivity, Linearity And Dispersion
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion ag-textnode-version-id: 3523939211 ag-textnode-base-id: 3523939211 Optimizing for Sensitivity, Selectivity, Linearity and Dispersion ag-textnode-version-id: 3526175371 ag-textnode-base-id: 3526175371 Flow Cell Path Length Lambert-Beer’s law shows a linear relationship between the flow cell path length and absorbance.
Page 87: Peak Width (Response Time)
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion As a rule-of-thumb the flow cell volume should be about 1/3 of the peak volume at half height. To determine the volume of your peaks, take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3).
Page 88 Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Response time = 2 s Response time = 0.016 s Slit width = 4 nm Figure 18: Influence of response time on signal and noise Table 16 on page 88 and Table 17 on page 89 list the filter choices of the detector.
Page 89 Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Peak width at half Response Signal data Scan data rate Scan data rate Scan data rate Scan data rate height [min] rate [Hz] [HZ] [HZ] [HZ] [HZ] ≤126 pts/scan ≤251 pts/scan ≤501 pts/scan...
Page 90: Sample And Reference Wavelength And Bandwidth
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion The maximum spectra scan rate depends on the data points per scan, see Table NOTE on page 88 and Table 17 on page 89. Running at 160 Hz, the spectra scan data rate is reduced automatically if the spectra scan data rate is more than 251 points/scan.
Page 91 Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion If you work with high concentrations, you may get better linearity above 1.5 AU by setting the sample wavelength to a valley in the spectrum, like 225 nm for anisic acid.
Page 92: Slit Width (G7117B)
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Bandwidth 30 nm 12 nm 4 nm Figure 20: Influence of bandwidth on signal and noise Because the detector averages absorbance values that are calculated for each wavelength, using a wide bandwidth does not negatively impact linearity. The use of a reference wavelength is highly recommended to further reduce baseline drift and wander induced by room temperature fluctuations or refractive index changes during a gradient.
Page 93 Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 4 nm 1 nm Figure 21: Benzene at 1 and 4 nm slit width (principle) A wide slit uses more of the light shining through the flow cell. This gives lower baseline noise as shown in.Figure 22 on page 93...
Page 94: Optimizing Spectral Acquisition
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Furthermore, the absorbance is no longer strictly linear with concentration for wavelengths at a steep slope of a compound’s spectrum. Substances with fine structures and steep slopes like benzene are very rare. In most cases the width of absorbance bands in the spectrum is more like 30 nm as with anisic acid ( Figure 19...
Page 95: Margin For Negative Absorbance
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion ag-textnode-version-id: 3358181387 ag-textnode-base-id: 3358181387 Margin for Negative Absorbance The detector adjusts its gain during balance such that the baseline may drift slightly negative (about -100 mAU). In some special case, for example, when gradient with absorbing solvents are used, the baseline may drift to more negative values.
Page 96: Optimizing Selectivity
Optimizing the Performance of the Module Optimizing Selectivity ag-textnode-version-id: 3523907723 ag-textnode-base-id: 3523907723 Optimizing Selectivity ag-textnode-version-id: 3358163851 ag-textnode-base-id: 3358163851 Quantifying Coeluting Peaks by Peak Suppression In chromatography, two compounds may often elute together. A conventional dual-signal detector can only detect and quantify both compounds independently from each other if their spectra do not overlap.
Page 97 Optimizing the Performance of the Module Optimizing Selectivity With a UV-visible detector based on a diode array and the correct choice of a reference wavelength setting, quantitative detection is possible. To suppress caffeine, the reference wavelength must be set to 282 nm. At this wavelength, caffeine shows exactly the same absorbance as at 222 nm.
Page 98: Optimizing The Detector Regarding To The System
The extra-column volume is minimized with the Agilent InfinityLab LC Series system due to the narrow-bore (0.12 mm i.d.) tubing, the low-volume heat exchangers in the column compartment and the flow cell in the detector.
Page 99: How To Achieve Higher Sensitivity
Optimizing the Performance of the Module Optimizing the Detector Regarding to the System ag-textnode-version-id: 3526157451 ag-textnode-base-id: 3526157451 How to Achieve Higher Sensitivity The detector has a number of parameters that are used to optimize performance. The following sections describe how the detector parameters affect performance characteristics: •...
Page 100 Optimizing the Performance of the Module Optimizing the Detector Regarding to the System For example, a signal at wavelength 250 nm with a bandwidth of 16 nm will be an average of the absorbance data from 242 nm to 258 nm. Additionally, a reference wavelength and reference bandwidth can be defined for each signal.
Page 101 Optimizing the Performance of the Module Optimizing the Detector Regarding to the System The reference bandwidth is normally set on a region of the UV spectrum in which the analyte has no absorbance. This is shown in the spectrum for anisic acid in Figure 25 on page 101.
Page 102 Optimizing the Performance of the Module Optimizing the Detector Regarding to the System The detector internally acquires data points faster than is needed for a chromatogram and processes them to produce the signal seen by the data system. Part of the processing reduces the data to an appropriate data rate which allows the chromatographic peaks to be accurately drawn.
Page 103 Optimizing the Performance of the Module Optimizing the Detector Regarding to the System Peak width at half Response Signal data Scan data rate Scan data rate Scan data rate Scan data rate height [min] rate [Hz] [HZ] [HZ] [HZ] [HZ] ≤126 pts/scan ≤251 pts/scan ≤501 pts/scan...
Page 104: Warm Up Of The Detector
Optimizing the Performance of the Module Warm up of the Detector ag-textnode-version-id: 3283635595 ag-textnode-base-id: 3283635595 Warm up of the Detector Give the optical unit enough time to warm-up and stabilize (> 60 minutes). The detector is temperature controlled. After turn-on of the detector, it goes through a cycle of different states: •...
Page 105 Optimizing the Performance of the Module Warm up of the Detector Figure 26: Detector Warm-up – 1 hour Figure 27: Detector Warm-up – 2 hour Diode Array Detectors User Manual...
Page 106: Diagnostics And Troubleshooting
ag-textnode-version-id: 18014404181184651 ag-textnode-base-id: 5671702667 Diagnostics and Troubleshooting This chapter gives an overview of the maintenance, troubleshooting, and diagnostic features available. Diagnostic Features 107 User Interfaces 107 Troubleshooting With HPLC Advisor 107 Overview of Available Tests and Tools 108 Tests and Calibrations in Agilent Lab Advisor 108 Maintenance and Troubleshooting Tools of the Module 109 Available Tests vs User Interfaces 109 Introduction 110...
Page 107: Diagnostic Features
Diagnostics and Troubleshooting Diagnostic Features ag-textnode-version-id: 27021601745768715 ag-textnode-base-id: 3981545739 Diagnostic Features This section gives an overview of the diagnostic features available. ag-textnode-version-id: 18014402312290571 ag-textnode-base-id: 3802808587 User Interfaces InfinityLab Assist InfinityLab Assist provides you with assisted troubleshooting and maintenance at your instrument. If the system in use supports the InfinityLab Assist, follow the instructions provided.
Page 108: Overview Of Available Tests And Tools
Diagnostics and Troubleshooting Overview of Available Tests and Tools ag-textnode-version-id: 4060343563 ag-textnode-base-id: 4060343563 Overview of Available Tests and Tools ag-textnode-version-id: 3002950539 ag-textnode-base-id: 3002950539 Tests and Calibrations in Agilent Lab Advisor Use the tests and diagnostic features provided in the Agilent Lab Advisor software to check if your module is working correctly.
Page 109: Maintenance And Troubleshooting Tools Of The Module
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 18014402571032715 ag-textnode-base-id: 4061550731 Maintenance and Troubleshooting Tools of the Module This chapter describes the tests for the module. ag-textnode-version-id: 3526215691 ag-textnode-base-id: 3526215691 Available Tests vs User Interfaces Depending on the used interface, the available tests and the screens/reports may NOTE vary.
Page 110: Introduction
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3526242059 ag-textnode-base-id: 3526242059 Introduction All tests are described based on the Agilent Lab Advisor Software B.02.08. Other user interfaces may not provide any test or just a few. For details on the use of the interface refer to the interface documentation. The Lab Advisor shows the available test under Service &...
Page 111 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Table 20: Interfaces and available test functions Interface Comment Available Function Agilent Lab Advisor Available functions depend on Product Level (Basic – For functions, refer to Function Advanced – FSE) Overview Lab Advisor •...
Page 112 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Functions Product Level Controls - Advanced Method Parameters 2 lamp required Advanced - Analog Output 1 Attenuation Advanced - Analog Output 1 Offset [% Full Advanced Scale] - Configuration - Remote Pulse Duration [s] * Basic Advanced - Analog Output 1 Range...
Page 113 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Functions Product Level - Signal A [mAU] Advanced Statemachines - UV Lamp Basic Advanced Signals - Signal A [mAU] Advanced - Lamp Voltage [V] Advanced - Board Temperature [°C] Advanced - Optical Temperature [°C] Advanced EMF Counters...
Page 114: Use Of Max-Light Cartridge Test Cell
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 28: The Lab Advisor shows the available test ag-textnode-version-id: 3526352523 ag-textnode-base-id: 3526352523 Use of Max-Light Cartridge Test Cell The Max-Light Cartridge Test Cell is recommended to be used for several tests instead of the Max-Light Cartridge Cell (10 mm, V( σ) = 1 µL) or the Max-Light Cartridge Cell (60 mm, V( σ) = 4 µL) because it allows running the test(s) without any influence of the rest of the system (degasser, pump, sampler and others).
Page 115: Conditions Of Detector
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module If the profile of the Max-Light Cartridge Cell differs in the low UV range, then absorbing solvents are in the cell and should be flushed out. See also Clean the Max-Light Cartridge Cell on page 189.
Page 116: Self-Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3526291723 ag-textnode-base-id: 3526291723 Self-Test The self-test runs a series of individual tests (described on the next pages), and evaluates the results automatically. The following tests are run: • Slit Test (G7117B only) •...
Page 117 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Self-Test with Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 29: Self-Test – Results Under the tab Signals you can find the detailed signals from the tests. Diode Array Detectors User Manual...
Page 118: Intensity Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 9007202780987531 ag-textnode-base-id: 3526246539 Intensity Test The intensity test measures the intensity of the UV-lamp over the full wavelength range (190 - 640 nm). Four spectral ranges are used to evaluate the intensity spectrum.
Page 119: Test Failed
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Intensity-Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 30: Intensity Test – Results Figure 31: Intensity Test – Signals ag-textnode-version-id: 9007202780991243 ag-textnode-base-id: 3526250251 Test Failed Intensity Test Evaluation Diode Array Detectors User Manual...
Page 120 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module If only one range fails and the application does not require this range, the lamp NOTE may not be changed. Probable cause Suggested actions Absorbing solvent or air • Ensure the flow cell is filled with water, and free from air bubble in flow cell.
Page 121: Cell Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3526357003 ag-textnode-base-id: 3526357003 Cell Test The cell test measures the intensity of the UV-lamp over the full wavelength range (190 - 690 nm), once with the Max-Light Cartridge Cell installed, and once with the Max-Light Cartridge Test Cell.
Page 122 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Cell-Test with Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 32: Cell Test – Results Figure 33: Cell Test – Signals (example shows low intensity for flow cell) ag-textnode-version-id: 3526360715 ag-textnode-base-id: 3526360715 Test Failed (low ratio value)
Page 123 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Absorbing solvent or air • Ensure the flow cell is filled with water, and free from air bubble in flow cell. bubbles. Dirty or contaminated flow •...
Page 124: Quick Noise Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3526295435 ag-textnode-base-id: 3526295435 Quick Noise Test The quick noise test measures the noise of the detector, with Max-Light Cartridge Cell or with Max-Light Cartridge Test Cell installed, in one minute intervals over a total of 5 minutes.
Page 125 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Quick Noise Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 34: Quick Noise Test – Results Figure 35: Quick Noise Test – Signal ag-textnode-version-id: 3526299147 ag-textnode-base-id: 3526299147 Test Failed...
Page 126 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Insufficient lamp warm-up • Allow detector and UV-lamp turned on for at least 2 time. hours. Absorbing solvent or air • Ensure the flow cell is filled with water, and free from air bubble in flow cell.
Page 127: Astm Drift Noise Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3526336907 ag-textnode-base-id: 3526336907 ASTM Drift Noise Test The ASTM noise test determines the detector noise over a period of 20 minutes. The test is done with installed Max-Light Cartridge Cell or Max-Light Cartridge Test Cell.
Page 128 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the ASTM Drift and Noise Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 36: ASTM Drift and Noise Test – Results Figure 37: ASTM Drift and Noise Test – Signal ag-textnode-version-id: 3526340619 ag-textnode-base-id: 3526340619 Test Failed...
Page 129: Slit Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Insufficient lamp warm-up • Allow detector and UV-lamp turned on for at least 2 time. hours. Absorbing solvent or air • Ensure the flow cell is filled with water, and free from air bubble in flow cell.
Page 130 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Slit Test with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 38: Slit Test – Results Figure 39: Slit Test – Signal ag-textnode-version-id: 3526368907 ag-textnode-base-id: 3526368907 Test Failed Slit Test Evaluation...
Page 131 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Defective slit assembly. • Please contact your Agilent service representative. Defective detector main • Please contact your Agilent service representative. board. Defective optical unit. • Please contact your Agilent service representative. ag-textnode-version-id: 3526385547 ag-textnode-base-id: 3526385547 Slit Test (G7117A/G7117C)
Page 132: Wavelength Verification Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3526348811 ag-textnode-base-id: 3526348811 Wavelength Verification Test The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of the UV-lamp for wavelength calibration. The sharp emission lines enable accurate calibration.
Page 133 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Wavelength Verification Test with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 40: Wavelength Verification – Results Diode Array Detectors User Manual...
Page 134: Wavelength Calibration
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3526390539 ag-textnode-base-id: 3526390539 Wavelength Calibration The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of the deuterium lamp for wavelength calibration. The sharp emission lines enable more accurate calibration than is possible with holmium oxide. When recalibration is started, the 1 nm slit is moved into the light path automatically (G7117B).
Page 135 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module If the detector was repaired (opened covers), the wavelength calibration can be NOTE done 10 minutes after lamp on. A final wavelength calibration should be repeated after complete warm-up of the detector. 1 Run the Wavelength Calibration with the Agilent Lab Advisor (for further information see Online-Help of user interface).
Page 136 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Absorbing solvent or air • Repeat calibration with Max-Light Cartridge Test Cell bubble in Max-Light and compare results. Cartridge Cell. Dirty or contaminated Max- • Ensure the Max-Light Cartridge Cell is filled with water. Light Cartridge Cell.
Page 137: D/A Converter (Dac) Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3526275339 ag-textnode-base-id: 3526275339 D/A Converter (DAC) Test The detector provides analog output of chromatographic signals for use with integrators, chart recorders or data systems. The analog signal is converted from the digital format by the digital-analog-converter (DAC).
Page 138 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the D/A Converter (DAC) Test with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 42: D/A Converter (DAC) Test – Results Figure 43: D/A Converter (DAC) Test – Example of Integrator Plot ag-textnode-version-id: 3284410763 ag-textnode-base-id: 3284410763 D/A Converter Test failed...
Page 139: Dark Current Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3526345099 ag-textnode-base-id: 3526345099 Dark Current Test The dark-current test measures the leakage current from each diode. The test is used to check for leaking diodes which may cause non-linearity at specific wavelengths.
Page 140 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358540299 ag-textnode-base-id: 3358540299 Dark-Current Test Failed Dark-Current Test evaluation Probable cause Suggested actions Defective slit assembly • Run the Self-Test on page 115. (stray light). • Run the Slit Test (G7117B) on page 129 (part of the Self- Test...
Page 141: Using The Built-In Test Chromatogram
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 18014401794165771 ag-textnode-base-id: 3284683787 Using the Built-In Test Chromatogram This function is available from the Agilent ChemStation, Lab Advisor and Instant Pilot. The built-in Test Chromatogram can be used to check the signal path from the detector to the data system and the data analysis or via the analog output to the integrator or data system.
Page 142 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 6 To start a test chromatogram enter in the command line: STRT Figure 46: Test Chromatogram with Agilent Lab Advisor 7 To stop the test chromatogram enter in the command line: STOP The test chromatogram is switched off automatically at the end of a run.
Page 143: Agilent Lab Advisor Software
Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument. The Lab Advisor Advanced features can be unlocked by purchasing a license key, and include real-time monitoring of instrument actuals, all various instrument signals, and state machines.
Page 144: Other Lab Advisor Functions
Diagnostics and Troubleshooting Other Lab Advisor Functions ag-textnode-version-id: 9007203316275083 ag-textnode-base-id: 4061534091 Other Lab Advisor Functions ag-textnode-version-id: 3526446219 ag-textnode-base-id: 3526446219 EMFs - Early Maintenance Feature The EMFs screen allows you to view and manage the EMF counters for all modules in all systems. Diode Array Detectors User Manual...
Page 145: Error Information
ag-textnode-version-id: 18014404181541003 ag-textnode-base-id: 5672059019 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. What Are Error Messages 146 General Error Messages 147 Timeout 147 Shutdown 147 Remote Timeout 148 Lost CAN Partner 149 Leak 150 Leak Sensor Open 151...
Page 146: What Are Error Messages
Error Information What Are Error Messages ag-textnode-version-id: 18014401511832075 ag-textnode-base-id: 3002350091 What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs that requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).
Page 147: General Error Messages
Error Information General Error Messages ag-textnode-version-id: 72057597564456331 ag-textnode-base-id: 3526528395 General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. ag-textnode-version-id: 9007202257100299 ag-textnode-base-id: 3002359307 Timeout Error ID: 62 The timeout threshold was exceeded.
Page 148: Shutdown
Error Information General Error Messages ag-textnode-version-id: 27021600766713739 ag-textnode-base-id: 3002490763 Shutdown Error ID: 63 An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.
Page 149: Remote Timeout
Error Information General Error Messages ag-textnode-version-id: 9007202257270027 ag-textnode-base-id: 3002529035 Remote Timeout Error ID: 70 A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis.
Page 150: Lost Can Partner
Error Information General Error Messages ag-textnode-version-id: 9007202257121675 ag-textnode-base-id: 3002380683 Lost CAN Partner Error ID: 71 During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.
Page 151: Leak
Error Information General Error Messages ag-textnode-version-id: 27021600766637707 ag-textnode-base-id: 3002414731 Leak Error ID: 64 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present.
Page 152: Leak Sensor Open
Error Information General Error Messages ag-textnode-version-id: 27021600766781835 ag-textnode-base-id: 3002558859 Leak Sensor Open Error ID: 83 The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 153: Leak Sensor Short
Error Information General Error Messages ag-textnode-version-id: 27021600766675979 ag-textnode-base-id: 3002453003 Leak Sensor Short Error ID: 82 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 154: Compensation Sensor Open
Error Information General Error Messages ag-textnode-version-id: 54043198531068427 ag-textnode-base-id: 3002622475 Compensation Sensor Open Error ID: 81 The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature.
Page 155: Compensation Sensor Short
Error Information General Error Messages ag-textnode-version-id: 54043198531110923 ag-textnode-base-id: 3002664971 Compensation Sensor Short Error ID: 80 The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature.
Page 156: Fan Failed
Error Information General Error Messages ag-textnode-version-id: 36028800302691851 ag-textnode-base-id: 3283727883 Fan Failed Error ID: 68 The cooling fan in the module has failed. Depending on the module, assemblies (e.g. the lamp in the detector) are turned off to assure that the module does not overheat inside. Probable cause Suggested actions Fan cable disconnected.
Page 157: Open Cover
Error Information General Error Messages ag-textnode-version-id: 9007202538630155 ag-textnode-base-id: 3283889163 Open Cover Error ID: 205 The top foam has been removed. The sensor on the main board detects when the top foam is in place. If the foam is removed, the fan is switched off, and the error message is generated. Probable cause Suggested actions The top foam was removed...
Page 158: Cover Violation
Error Information General Error Messages ag-textnode-version-id: 18014401793749899 ag-textnode-base-id: 3284267915 Cover Violation Error ID: 7461 The top foam has been removed. The sensor on the main board detects when the top foam is in place. If the foam is removed while the lamps are on (or if an attempt is made to switch on for example the lamps with the foam removed), the lamps are switched off, and the error message is generated.
Page 159: Eri Messages
Error Information General Error Messages ag-textnode-version-id: 9007202538689547 ag-textnode-base-id: 3283948555 ERI Messages Error ID: 11120, 11121 The ERI (Enhanced Remote Interface) provides two error events related to over current situations on the +5 V and +24 V lines. Probable cause Suggested actions The load on the ERI is too •...
Page 160: Detector Error Messages
Error Information Detector Error Messages ag-textnode-version-id: 9007204926804363 ag-textnode-base-id: 5672063371 Detector Error Messages These errors are detector specific. ag-textnode-version-id: 9007202614134667 ag-textnode-base-id: 3359393675 Diode Current Leakage Error ID: 1041 When the detector is switched on, the processor checks the leakage current of each of the optical diodes.
Page 161: Uv Lamp: No Current
Error Information Detector Error Messages ag-textnode-version-id: 9007202538702475 ag-textnode-base-id: 3283961483 UV Lamp: No Current Error ID: 7450 The lamp anode current is missing. The processor continually monitors the anode current drawn by the lamp during operation. If the anode current falls below the lower current limit, the error message is generated.
Page 162: Uv Lamp: No Voltage
Error Information Detector Error Messages ag-textnode-version-id: 9007202538757643 ag-textnode-base-id: 3284016651 UV Lamp: No Voltage Error ID: 7451 The lamp anode voltage is missing. The processor continually monitors the anode voltage across the lamp during operation. If the anode voltage falls below the lower limit, the error message is generated.
Page 163: Lamp Ignition Failed
Error Information Detector Error Messages ag-textnode-version-id: 9007202538774795 ag-textnode-base-id: 3284033803 Lamp Ignition Failed Error ID: 7452 The lamp failed to ignite. The processor monitors the lamp current during the ignition cycle. If the lamp current does not rise above the lower limit within 2 –...
Page 164: No Heater Current
Error Information Detector Error Messages ag-textnode-version-id: 9007202538796171 ag-textnode-base-id: 3284055179 No Heater Current Error ID: 7453 The lamp heater current in the detector is missing. During lamp ignition, the processor monitors the heater current. If the current does not rise above the lower limit within 1 , the error message is generated.
Page 165: Calibration Values Invalid
Error Information Detector Error Messages ag-textnode-version-id: 9007202614292235 ag-textnode-base-id: 3359551243 Calibration Values Invalid Error ID: 1036 The calibration values read from the spectrometer ROM are invalid. After recalibration, the calibration values are stored in ROM. The processor periodically checks if the calibration data are valid. If the data are invalid or cannot be read from the spectrometer ROM, the error message is generated.
Page 166: Wavelength Recalibration Lost
Error Information Detector Error Messages ag-textnode-version-id: 9007202614368779 ag-textnode-base-id: 3359627787 Wavelength Recalibration Lost Error ID: 1037 The calibration information needed for your detector to operate correctly has been lost. During calibration of the detector the calibration values are stored in ROM. If no data is available in the spectrometer ROM, the error message is generated.
Page 167: Illegal Temperature Value From Sensor On Main Board
Error Information Detector Error Messages ag-textnode-version-id: 9007202539118987 ag-textnode-base-id: 3284377995 Illegal Temperature Value from Sensor on Main Board Error ID: 1071 This temperature sensor (located on the detector main board) delivered a value outside the allowed range. The parameter of this event equals the measured temperature in 1/100 centigrade.
Page 168: Illegal Temperature Value From Sensor At Air Inlet
Error Information Detector Error Messages ag-textnode-version-id: 9007202539089163 ag-textnode-base-id: 3284348171 Illegal Temperature Value from Sensor at Air Inlet Error ID: 1072 This temperature sensor delivered a value outside the allowed range. The parameter of this event equals the measured temperature in 1/100 centigrade. As a result the temperature control is switched off.
Page 169: Heater At Fan Assembly Failed
Error Information Detector Error Messages ag-textnode-version-id: 9007202538979083 ag-textnode-base-id: 3284238091 Heater at Fan Assembly Failed Error ID: 1073 Every time the deuterium lamp or the tungsten lamp (DAD only) is switched on or off a heater self-test is performed. If the test fails an error event is created. As a result the temperature control is switched off.
Page 170: Heater Power At Limit
Error Information Detector Error Messages ag-textnode-version-id: 9007202538936331 ag-textnode-base-id: 3284195339 Heater Power at Limit Error ID: 1074 The available power of the heater reached either the upper or lower limit. This event is sent only once per run. The parameter determines which limit has been hit: 0 means upper power limit hit (excessive ambient temperature drop).
Page 171: Maintenance
ag-textnode-version-id: 27021601842266251 ag-textnode-base-id: 4078043275 Maintenance This chapter provides general information on maintenance of the module. Introduction to Maintenance 172 Safety Information Related to Maintenance 173 Overview of Maintenance 175 Cleaning the Module 176 Remove and Install Doors 177 Replace the Deuterium Lamp 180 Replace the Max-Light Cartridge Cell 184 Clean the Max-Light Cartridge Cell 189 Storage of Max-Light Cartridge Cell 190 Correcting Leaks 191...
Page 172: Introduction To Maintenance
Maintenance Introduction to Maintenance ag-textnode-version-id: 3284748811 ag-textnode-base-id: 3284748811 Introduction to Maintenance The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system. There are no serviceable parts inside. NOTE Do not open the module. ag-struc-frag-safety-version-id: 3369125003 ag-struc-frag-safety-base-id: 3369125003 Diode Array Detectors User Manual...
Page 173: Safety Information Related To Maintenance
Maintenance Safety Information Related to Maintenance ag-textnode-version-id: 18014402382780683 ag-textnode-base-id: 3873298699 Safety Information Related to Maintenance Eye damage by detector light WARNING ag-struc-frag-safety-version-id: 9007202540437131 ag-struc-frag-safety-base-id: 3285696139 Eye damage may result from directly viewing the UV-light produced by the lamp of the optical system used in this product. —...
Page 174 Maintenance Safety Information Related to Maintenance Toxic, flammable and hazardous solvents, samples and reagents WARNING ag-struc-frag-safety-version-id: 3003809803 ag-struc-frag-safety-base-id: 3003809803 The handling of solvents, samples and reagents can hold health and safety risks. — When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 175: Overview Of Maintenance
Maintenance Overview of Maintenance ag-textnode-version-id: 3526830859 ag-textnode-base-id: 3526830859 Overview of Maintenance The following pages describe maintenance (simple repairs) of the detector that can be carried out without opening the main cover. Table 23: Overview of Maintenance Procedure Typical Frequency Notes Cleaning of module If required Deuterium lamp If noise and/or drift exceeds your application limits or...
Page 176: Cleaning The Module
Maintenance Cleaning the Module ag-textnode-version-id: 3002023307 ag-textnode-base-id: 3002023307 Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Avoid using organic solvents for cleaning purposes. They can cause damage to plastic parts. Liquid dripping into the electronic compartment of your module can cause WARNING shock hazard and damage the module...
Page 177: Remove And Install Doors
Maintenance Remove and Install Doors ag-textnode-version-id: 18014402225732235 ag-textnode-base-id: 3716250251 Remove and Install Doors When • The instrument doors or the hinges are broken. Tools required Qty. Description 5023-3138 Reversible Screwdriver + Blade 1,0 x 5,5 Parts required Qty. Description (Infinity III) 5004-3140 Door Kit Infinity III 140mm Parts required...
Page 178 Maintenance Remove and Install Doors 1 Press the release buttons and pull the front door out. 2 For the Installation of the front door, insert the hinges into their guides and push the door in until the release buttons click into their final position. Diode Array Detectors User Manual...
Page 179 Maintenance Remove and Install Doors Diode Array Detectors User Manual...
Page 180: Replace The Deuterium Lamp
Maintenance Replace the Deuterium Lamp ag-textnode-version-id: 9007202781580043 ag-textnode-base-id: 3526839051 Replace the Deuterium Lamp When • If noise or drift exceeds application limits or lamp does not ignite. Tools required Qty. Description Screwdriver POZI 1 PT3 Parts required Qty. Description 5190-0917 Long-life Deuterium lamp (8-pin) with RFID tag Preparations •...
Page 181 Maintenance Replace the Deuterium Lamp 2 Locate the lamp cover. 3 Grab the lamp cover and pull it off (it is fixed by a magnet in the bottom of the cover). 4 Unplug the lamp connector (1.) and unscrew the two lamp screws (2.) (Pozidriv).
Page 182 Maintenance Replace the Deuterium Lamp 5 Remove the lamp and place it on a clean place. Do not touch the glass bulb with your fingers. It may reduce the light output. NOTE 6 Insert the lamp (RFID tag on the right side). 7 Fix the lamp screws (1.) and reconnect the lamp connector (2.).
Page 183 Maintenance Replace the Deuterium Lamp 8 Place the lamp cable in the lamp cover. 9 Slide the lamp cover into the top position of the metal front (1.) and press the lamp cover completely in until it clicks (2.). 10 Close the doors. 11 Perform a Wavelength Re-calibration after lamp warm-up.
Page 184: Replace The Max-Light Cartridge Cell
Maintenance Replace the Max-Light Cartridge Cell ag-textnode-version-id: 9007202781640203 ag-textnode-base-id: 3526899211 Replace the Max-Light Cartridge Cell When • If leaking or if intensity drops due to contaminated flow cell. Tools required Qty. Description Wrench, 1/4 inch for capillary connections Parts required Qty. Description G4212-60008 Max-Light Cartridge Cell (10 mm, V(σ) 1.0 μL)
Page 185 Maintenance Replace the Max-Light Cartridge Cell 2 Disconnect the capillaries from the flow cell cartridge. 3 Flip the cartridge lever towards the front (down). Diode Array Detectors User Manual...
Page 186 Maintenance Replace the Max-Light Cartridge Cell 4 Pull the cartridge holder completely out. 5 Remove the flow cell cartridge. Install the Max-Light Cartridge Cell 1 Insert the flow cell cartridge. Diode Array Detectors User Manual...
Page 187 Maintenance Replace the Max-Light Cartridge Cell 2 Slide the cartridge holder completely into the detector. 3 Lift the cartridge lever up into the final position. 4 Reconnect the capillaries. Diode Array Detectors User Manual...
Page 188 Maintenance Replace the Max-Light Cartridge Cell 5 Close the doors. 6 Perform a Cell Test and a Wavelength Verification Test. Diode Array Detectors User Manual...
Page 189: Clean The Max-Light Cartridge Cell
Maintenance Clean the Max-Light Cartridge Cell ag-textnode-version-id: 3526876939 ag-textnode-base-id: 3526876939 Clean the Max-Light Cartridge Cell When • Low counts on Intensity Test or Cell Test (failed tests) Tools required Qty. Description Alcohol (Iso-propanol or Ethanol) Lens tissue or Q-tips ® 5190-0530 Cell cleaning solvent 1 Flush the flow cell with the alcohol for some time.
Page 190: Storage Of Max-Light Cartridge Cell
Maintenance Storage of Max-Light Cartridge Cell ag-textnode-version-id: 3526835339 ag-textnode-base-id: 3526835339 Storage of Max-Light Cartridge Cell 1 Flush the Max- Light Cartridge Flow Cell with iso- propanol or methanol and insert the plugs into the cell inlet and outlet (see Replace the Max-Light Cartridge Cell on page 184).
Page 191: Correcting Leaks
Maintenance Correcting Leaks ag-textnode-version-id: 9007202781583755 ag-textnode-base-id: 3526842763 Correcting Leaks When • If leak has occurred. Tools required Qty. Description Tissue Preparations • Turn the pump off. 1 Open the doors. Diode Array Detectors User Manual...
Page 192 Maintenance Correcting Leaks 2 Use tissue to dry the leak sensor area (1). Observe the capillary connections and the flow cell area (2) for leaks and correct, if required. 3 Close the doors. Diode Array Detectors User Manual...
Page 193: Replace Leak Handling System Parts
Maintenance Replace Leak Handling System Parts ag-textnode-version-id: 9007202781587467 ag-textnode-base-id: 3526846475 Replace Leak Handling System Parts Parts required Qty. Description 5043-0856 Leak Adapter 5063-6527 Tubing, Silicon Rubber, 1.2 m, ID/OD 6 mm/ 9 mm approximately 85 mm required 1 Open the doors. 2 Locate the Leak Adapter (1) and Tubing (2). Diode Array Detectors User Manual...
Page 194 Maintenance Replace Leak Handling System Parts 3 Press the Leak Adapter down and remove it together with the tubing. 4 Install the Leak Adapter by pressing it into the Main Cover. Diode Array Detectors User Manual...
Page 195 Maintenance Replace Leak Handling System Parts 5 Insert the Tubing (approximately 85 mm required for replacement) between Leak Adapter outlet and Leak Panel. 6 Close the doors. Diode Array Detectors User Manual...
Page 196: Replace The Module Firmware
Maintenance Replace the Module Firmware ag-textnode-version-id: 18014401511543691 ag-textnode-base-id: 3002061707 Replace the Module Firmware When Install a newer firmware • It fixes known problems of older versions, or • It introduces new features, or • It ensures keeping all systems at the same (validated) revision When Install an older firmware •...
Page 197: Module Specific Information
Maintenance Replace the Module Firmware Module Specific Information Table 24: Module specific information (G7117 A/G7117B/G7117C) G7117B 1290 DAD G7117A 1290 DAD G7117C 1260 DAD Initial firmware (main D.06.07 D.07.01 and resident) Compatibility with When using the detector in a system, all other modules must have 1100/1200/1260/ firmware from set 6.50 (latest version) or later (main and resident).
Page 198: Information From Module's Assemblies
Maintenance Information from Module’s Assemblies ag-textnode-version-id: 3524894859 ag-textnode-base-id: 3524894859 Information from Module’s Assemblies ag-textnode-version-id: 3526850187 ag-textnode-base-id: 3526850187 Lamp and Flow Cell RFID Tag The detector is equipped with a UV lamp and flow cell identification system using RFID (radio frequency identification) tags attached to the assemblies and RFID tag readers at the optical unit.
Page 199: Parts And Materials For Maintenance
ag-textnode-version-id: 27021601842595467 ag-textnode-base-id: 4078372491 Parts and Materials for Maintenance This chapter provides information on parts for maintenance. Overview of Maintenance Parts 200 Accessory Kit (G7117-68755) 202 Diode Array Detectors User Manual...
Page 200: Overview Of Maintenance Parts
Parts and Materials for Maintenance Overview of Maintenance Parts ag-textnode-version-id: 9007202781462539 ag-textnode-base-id: 3526721547 Overview of Maintenance Parts Figure 47: Maintenance Parts Qty. Description 5043-0856 Leak Adapter 5063-6527 Tubing, Silicon Rubber, 1.2 m, ID/OD 6 mm/9 mm for Waste and Leak Adapter (ca. 85 mm required) 5062-8535 Waste accessory kit (Flow Cell to waste) (Flow Cell to waste)
Page 201 Parts and Materials for Maintenance Overview of Maintenance Parts Qty. Description 5360-0003 Door 140mm right Infinity II (only orderable as part of 5004-0140 Door Kit Infinity II 140mm) 5360-0002 Door 140mm left Infinity II (only orderable as part of 5004-0140 Door Kit Infinity II 140mm) 5190-0917...
Page 202: Accessory Kit (G7117-68755)
Parts and Materials for Maintenance Accessory Kit (G7117-68755) ag-textnode-version-id: 9007202781471499 ag-textnode-base-id: 3526730507 Accessory Kit (G7117-68755) Description 5062-8535 Waste accessory kit (Flow Cell to waste) 5063-6527 Tubing, Silicon Rubber, 1.2 m, ID/OD 6 mm/9 mm see item 4 in Figure 47 on page 200 5181-1516 CAN cable, Agilent module to module, 0.5 m 5500-1155 Tube Connector, 90 degree, ID 6.4 5043-1013...
Page 203: Identifying Cables
ag-textnode-version-id: 27021601743836299 ag-textnode-base-id: 3979613323 Identifying Cables This chapter provides information on cables used with the modules. Cable Overview 204 Analog Cables 206 Remote Cables 208 BCD Cables 212 CAN/LAN Cables 214 RS-232 Cables 215 USB 216 Diode Array Detectors User Manual...
Page 204: Cable Overview
Identifying Cables Cable Overview ag-textnode-version-id: 9007202256869899 ag-textnode-base-id: 3002128907 Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
Page 205 Identifying Cables Cable Overview RS-232 cables Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
Page 206: Analog Cables
Identifying Cables Analog Cables ag-textnode-version-id: 9007202256873995 ag-textnode-base-id: 3002133003 Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
Page 207 Identifying Cables Analog Cables Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Diode Array Detectors User Manual...
Page 208: Remote Cables
Identifying Cables Remote Cables ag-textnode-version-id: 9007202256882571 ag-textnode-base-id: 3002141579 Remote Cables ERI (Enhanced Remote Interface) • 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end) • 5188-8044 ERI to ERI (D_Sub 15 pin male - male) • 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female) p/n 5188-8029 Color code Enhanced Remote Classic Remote...
Page 209 Identifying Cables Remote Cables • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding Diode Array Detectors User Manual...
Page 210 Ground Cable Shielding One end of these cables provides an Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Diode Array Detectors User Manual...
Page 211 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 A/D Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue 4 - Blue Shut down 5 - Pink...
Page 212: Bcd Cables
Identifying Cables BCD Cables ag-textnode-version-id: 3358242315 ag-textnode-base-id: 3358242315 BCD Cables One end of these cables provides a 15-pin BCD connector to be connected to the Agilent modules. The other end depends on the instrument to be connected to Agilent Module to General Purpose p/n G1351-81600 Wire Color Pin Agilent...
Page 213 Identifying Cables BCD Cables Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Diode Array Detectors User Manual...
Page 214: Can/Lan Cables
Identifying Cables CAN/LAN Cables ag-textnode-version-id: 18014401511606795 ag-textnode-base-id: 3002124811 CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. Can Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description...
Page 215: Rs-232 Cables
Identifying Cables RS-232 Cables ag-textnode-version-id: 9007202256878091 ag-textnode-base-id: 3002137099 RS-232 Cables Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
Page 216: Usb
Identifying Cables ag-textnode-version-id: 9007202256886667 ag-textnode-base-id: 3002145675 To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Diode Array Detectors User Manual...
Page 217: Hardware Information
ag-textnode-version-id: 18014404181587211 ag-textnode-base-id: 5672105227 Hardware Information This chapter describes the module in more detail on hardware and electronics. General Hardware Information 218 Firmware Description 218 Electrical Connections 220 Interfaces 222 Instrument Layout 229 Early Maintenance Feedback (EMF) 230 Module-Specific Hardware Information 232 Setting the 6-bit Configuration Switch 232 Diode Array Detectors User Manual...
Page 218: General Hardware Information
Hardware Information General Hardware Information ag-textnode-version-id: 36028801083399691 ag-textnode-base-id: 4064435723 General Hardware Information This section provides detailed hardware information on firmware that is valid for this module. ag-textnode-version-id: 3002159627 ag-textnode-base-id: 3002159627 Firmware Description The firmware of the instrument consists of two independent sections: •...
Page 219: Firmware Updates
Hardware Information General Hardware Information • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Firmware Updates Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used).
Page 220: Electrical Connections
One analog output provides signals for integrators or data handling systems. • The ERI connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start, stop, common shut down, prepare, and so on. Diode Array Detectors User Manual...
Page 221: Rear View Of The Module
— Any other instruments connected to this instrument shall be approved to a suitable safety standard and must include reinforced insulation from the mains. Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations.
Page 222: Serial Number Information
(there can be more than one code for the same module) 00000 Serial number ag-textnode-version-id: 18014402069660043 ag-textnode-base-id: 3560178059 Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 27: Agilent InfinityLab LC Series interfaces Module RS-232 Analog APG (A) / Special...
Page 223 Hardware Information General Hardware Information Module RS-232 Analog APG (A) / Special (on-board) ERI (E) G7157A Detectors G7114A/B G7115A G7117A/B/C G7121A/B G7162A/B G7165A Fraction Collectors G7158B G7159B G7166A Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with additional G1369C LAN Card G1364E/F, G5664B...
Page 224: Overview Interfaces
Hardware Information General Hardware Information LAN connection is made between at least one of the Agilent modules and the NOTE Control PC. — If an Assist Hub is installed, connect the LAN to the Lab LAN port of this module. —...
Page 225 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
Page 226: Special Interfaces
Hardware Information General Hardware Information for next analysis, followed by START of run and optional STOP of run triggered on the respective lines. In addition PREPARE and START REQUEST may be issued. The signal levels are defined as: • standard TTL levels (0 V is logic true, + 5.0 V is false), •...
Page 227 ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the communication board core electronics use ERI. This interface is already used in the Agilent Universal Interface Box 2 (UIB2)
Page 228 Hardware Information General Hardware Information Enhanced Remote PGND +24 V ERI out +24 V ERI out IO (Input/Output) Lines • Eight generic bi-directional channels (input or output). • Same as the APG Remote. • Devices like valves, relays, ADCs, DACs, controllers can be supported/ controlled.
Page 229: Instrument Layout
Hardware Information General Hardware Information • If a connected device requires more power the firmware detects this (overcurrent detection) and provides the information to the user interface. • Fuse used for safety protection (on board). • Short circuit will be detected through hardware. ag-textnode-version-id: 3002198795 ag-textnode-base-id: 3002198795 USB (Universal Serial Bus)
Page 230: Early Maintenance Feedback (Emf)
Hardware Information General Hardware Information ag-textnode-version-id: 3002155147 ag-textnode-base-id: 3002155147 Early Maintenance Feedback (EMF) Maintenance requires the exchange of components that are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of use of the module and the analytical conditions, and not on a predefined time interval.
Page 231 Hardware Information General Hardware Information displayed values) as EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Diode Array Detectors User Manual...
Page 232: Module-Specific Hardware Information
Hardware Information Module-Specific Hardware Information ag-textnode-version-id: 18014402588071691 ag-textnode-base-id: 4078589707 Module-Specific Hardware Information ag-textnode-version-id: 18014402036253963 ag-textnode-base-id: 3526771979 Setting the 6-bit Configuration Switch The 6-bit configuration switch is located at the rear of the module with communication board electronics. Switch settings provide configuration parameters for LAN and instrument specific initialization procedures.
Page 233: Special Settings
Hardware Information Module-Specific Hardware Information Mode Init Mode Test Boot Main System/Revert to Default Data Test Boot Resident System/Revert to Default Data Legend: 0 (switch down), 1 (switch up), SW (switch) ag-textnode-version-id: 3285054731 ag-textnode-base-id: 3285054731 Special Settings Boot-Resident/Main Firmware update procedures may require this mode in case of firmware loading errors (main/resident firmware part).
Page 234 Hardware Information Module-Specific Hardware Information Loss of data CAUTION ag-struc-frag-safety-version-id: 3182477195 ag-struc-frag-safety-base-id: 3182477195 Forced cold start erases all methods and data stored in the non-volatile memory. Exceptions are calibration settings, diagnosis and repair log books which will not be erased. —...
Page 235: Lan Configuration
ag-textnode-version-id: 27021601047391371 ag-textnode-base-id: 3283168395 LAN Configuration This chapter provides information on connecting the module to the control software. What You Have to Do First 236 TCP/IP Parameter Configuration 237 Configuration Switch 238 Initialization Mode Selection 239 Dynamic Host Configuration Protocol (DHCP) 241 General Information (DHCP) 241 Setup (DHCP) 241 Manual Configuration 244 With Telnet 244...
Page 236: What You Have To Do First
LAN Configuration What You Have to Do First ag-textnode-version-id: 3285075339 ag-textnode-base-id: 3285075339 What You Have to Do First The module has an on-board LAN communication interface. This chapter is generic and may show figures that differ from your module. The NOTE functionality is the same.
Page 237: Tcp/Ip Parameter Configuration
LAN Configuration TCP/IP Parameter Configuration ag-textnode-version-id: 3285094667 ag-textnode-base-id: 3285094667 TCP/IP Parameter Configuration To operate properly in a network environment, the LAN interface must be configured with valid TCP/IP network parameters. These parameters are: • IP address • Subnet Mask • Default Gateway The TCP/IP parameters can be configured by the following methods: •...
Page 238: Configuration Switch
LAN Configuration Configuration Switch ag-textnode-version-id: 3285079051 ag-textnode-base-id: 3285079051 Configuration Switch The configuration switch can be accessed at the rear of the module. Configuration switch Figure 54: Location of configuration switch The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF.
Page 239: Initialization Mode Selection
LAN Configuration Initialization Mode Selection ag-textnode-version-id: 3285107339 ag-textnode-base-id: 3285107339 Initialization Mode Selection The following initialization (init) modes are selectable: Table 30: Initialization mode switches Init Mode Use Default IP Address Use Stored IP Address Use DHCP Note: The setting ‘0’ (down) is essential. Legend: 0 (switch down), 1 (switch up), SW (switch) Default IP address for LAN is 192.168.254.11.
Page 240: Using Default
LAN Configuration Initialization Mode Selection Using Default When Using Default is selected, the factory default parameters are taken instead. These parameters enable a TCP/IP connection to the LAN interface without further configuration, see Table 31 on page 240. Active Default Parameter Parameter Figure 56: Using Default (principle) Using the default address in your local area network may result in network...
Page 241: Dynamic Host Configuration Protocol (Dhcp)
LAN Configuration Dynamic Host Configuration Protocol (DHCP) ag-textnode-version-id: 3560226315 ag-textnode-base-id: 3560226315 Dynamic Host Configuration Protocol (DHCP) ag-textnode-version-id: 3285099147 ag-textnode-base-id: 3285099147 General Information (DHCP) The Dynamic Host Configuration Protocol (DHCP) is an auto configuration protocol used on IP networks. The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware.
Page 242: Setup (Dhcp)
LAN Configuration Dynamic Host Configuration Protocol (DHCP) ag-textnode-version-id: 3285103627 ag-textnode-base-id: 3285103627 Setup (DHCP) The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware. All modules should use latest firmware from the same set.
Page 243 LAN Configuration Dynamic Host Configuration Protocol (DHCP) Table 33: LC Modules with 8-bit configuration switch (B-firmware) (configuration switch at rear of the instrument) SW 6 SW 7 SW 8 Initialization Mode DHCP 3 Turn on the module that hosts the LAN interface. 4 Configure your Control Software (e.g.
Page 244: Manual Configuration
LAN Configuration Manual Configuration ag-textnode-version-id: 9007202781250955 ag-textnode-base-id: 3526509963 Manual Configuration Manual configuration only alters the set of parameters stored in the non-volatile memory of the module. It never affects the currently active parameters. Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing it.
Page 245: With Telnet
LAN Configuration Manual Configuration ag-textnode-version-id: 3285087243 ag-textnode-base-id: 3285087243 With Telnet Whenever a TCP/IP connection to the module is possible (TCP/IP parameters set by any method), the parameters may be altered by opening a Telnet session. 1 Open the system (DOS) prompt window by clicking on Windows START button and select “Run...”.
Page 246 LAN Configuration Manual Configuration Table 34: Telnet commands Value Description displays syntax and descriptions of commands displays current LAN settings ip <x.x.x.x> sets new ip address sm <x.x.x.x> sets new subnet mask gw <x.x.x.x> sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style: •...
Page 247 LAN Configuration Manual Configuration change of IP setting to Initialization mode is Using Stored active TCP/IP settings stored TCP/IP settings in non-volatile memory connected to PC with controller software (e.g. Agilent ChemStation), here not Telnet - Change IP settings connected 7 When you have finished typing the configuration parameters, type exit press Enter to exit with storing parameters.
Page 248: With The Instant Pilot (G4208A)
LAN Configuration Manual Configuration ag-textnode-version-id: 3285090955 ag-textnode-base-id: 3285090955 With the Instant Pilot (G4208A) To configure the TCP/IP parameters before connecting the module to the network, the Instant Pilot (G4208A) can be used. 1 From the Welcome screen press the More button. 2 Select Configure.
Page 249: Pc And User Interface Software Setup
LAN Configuration PC and User Interface Software Setup ag-textnode-version-id: 3595223819 ag-textnode-base-id: 3595223819 PC and User Interface Software Setup ag-textnode-version-id: 3596853899 ag-textnode-base-id: 3596853899 PC Setup for Local Configuration This procedure describes the change of the TCP/IP settings on your PC to match the module’s default parameters in a local configuration (see Table 31 page 240).
Page 250 LAN Configuration PC and User Interface Software Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration. Diode Array Detectors User Manual...
Page 251 LAN Configuration PC and User Interface Software Setup 3 We will use the direct LAN access via Cross-over LAN cable with the module’s IP address. 4 Click on OK to save the configuration. Diode Array Detectors User Manual...
Page 252: Appendix
Magnets 258 Safety Symbols 258 Material Information 261 General Information About Solvent/Material Compatibility 261 Flow Cell 266 At-a-Glance Details About Agilent Capillaries 267 Waste Electrical and Electronic Equipment (WEEE) Directive 271 Radio Interference 272 RFID Statement 273 Sound Emission 275 Agilent Technologies on Internet 276 Diode Array Detectors User Manual...
Page 253: General Safety Information
Appendix General Safety Information ag-textnode-version-id: 63050397785402635 ag-textnode-base-id: 3002215691 General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
Page 254: Before Applying Power
— Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. — Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Page 255: Do Not Operate In An Explosive Atmosphere
Appendix General Safety Information ag-textnode-version-id: 3002235275 ag-textnode-base-id: 3002235275 Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes WARNING ag-struc-frag-safety-version-id: 3004420107 ag-struc-frag-safety-base-id: 3004420107 Explosion hazard — Do not operate the instrument in the presence of flammable gases or fumes.
Page 256: Solvent Information
Appendix General Safety Information ag-textnode-version-id: 18014402035996427 ag-textnode-base-id: 3526514443 Solvent Information Toxic, flammable and hazardous solvents, samples and reagents WARNING ag-struc-frag-safety-version-id: 3004430475-2 ag-struc-frag-safety-base-id: 3004430475 The handling of solvents, samples and reagents can hold health and safety risks. — When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 257 Appendix General Safety Information Recommendations on the Use of Solvents Observe the following recommendations on the use of solvents. • Brown glass ware can avoid growth of algae. • Follow the recommendations for avoiding the growth of algae, see the pump manuals.
Page 258: Magnets
Appendix General Safety Information • If the flow cell is transported while temperatures are below 5 degree C, it must be assured that the cell is filled with alcohol. • Aqueous solvents in the flow cell can built up algae. Therefore do not leave aqueous solvents sitting in the flow cell.
Page 259 Appendix General Safety Information The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up. Indicates flammable material used. Consult the Agilent Information Center / User Manual before attempting to install or service this equipment. Follow all safety precautions.
Page 260 Appendix General Safety Information A WARNING WARNING ag-struc-frag-safety-version-id: 3004433931 ag-struc-frag-safety-base-id: 3004433931 alerts you to situations that could cause physical injury or death. — Do not proceed beyond a warning until you have fully understood and met the indicated conditions. A CAUTION CAUTION ag-struc-frag-safety-version-id: 3004437387 ag-struc-frag-safety-base-id: 3004437387...
Page 261: Material Information
Appendix Material Information ag-textnode-version-id: 45036002195549963 ag-textnode-base-id: 5921845003 Material Information This section provides detailed information about materials used in the HPLC system and general information about solvent/material compatibility. ag-textnode-version-id: 3002994315 ag-textnode-base-id: 3002994315 General Information About Solvent/Material Compatibility Materials in the flow path are carefully selected based on Agilent’s experiences in developing highest-quality instruments for HPLC analysis over several decades.
Page 262 Appendix Material Information resistance to high-temperature oxidation. Due to excellent chemical resistance and toughness, the alloy is used in diverse applications: dental products, medical devices, nonmagnetic electrical components, chemical and food processing equipment, marine equipment. Treatment of MP35N alloy samples with 10 % NaCl in HCl (pH 2.0 ) does not reveal any detectable corrosion.
Page 263 Appendix Material Information When used above room temperature, PEEK is sensitive to bases and various organic solvents, which can cause it to swell. Under such conditions, normal PEEK capillaries are sensitive to high pressure. Therefore, Agilent uses stainless steel clad PEEK capillaries in bio-inert systems. The use of stainless steel clad PEEK capillaries keeps the flow path free of steel and ensures pressure stability up to 600 bar.
Page 264 Appendix Material Information Stainless Steel (SST) Stainless steel is inert against many common solvents. It is stable in the presence of acids and bases in a pH range of 1 to 12.5 . It can be corroded by acids below pH 2.3 . It can also corrode in following solvents: •...
Page 265 Appendix Material Information Titanium is subject to corrosion in anhydrous methanol, which can be avoided by adding a small amount of water (about 3 %). Slight corrosion is possible with ammonia > 10 %. Diamond-Like Carbon (DLC) Diamond-Like Carbon is inert to almost all common acids, bases, and solvents. There are no documented incompatibilities for HPLC applications.
Page 266: Flow Cell
Appendix Material Information Fluorinated Polymers (PTFE, PFA, FEP, FFKM, PVDF) Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy), and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to most chemicals.
Page 267: At-A-Glance Details About Agilent Capillaries
Appendix At-a-Glance Details About Agilent Capillaries ag-textnode-version-id: 18014401512496395 ag-textnode-base-id: 3003014411 At-a-Glance Details About Agilent Capillaries The following section provides useful information about Agilent capillaries and its characteristics. Syntax for capillary description Type - Material - Capillary dimensions - Fitting Left/Fitting right Table 36: Example for a capillary description Code provided Meaing of the code...
Page 268 Appendix At-a-Glance Details About Agilent Capillaries Color Coding Guide Table 37: Color-coding key for Agilent capillary tubing Internal diameter in mm Color code 0.015 Orange 0.025 Yellow 0.05 Beige 0.075 Black 0.075 MP35N Black with orange stripe 0.1 Purple 0.12 0.12 MP35N Red with orange stripe 0.17 ...
Page 269 Appendix At-a-Glance Details About Agilent Capillaries Description Tube Tubing Heat exchanger Heat exchanger Abbreviation Guide for Material Table 39: Material (indicates which raw material is used for the capillary) Description Stainless steel Titanium PEEK FS/PK PEEK-coated fused silica PK/ST Stainless steel-coated PEEK PFFE PTFE Fused silica...
Page 270 Appendix At-a-Glance Details About Agilent Capillaries Abbreviation Guide for Fitting Left/Fitting Right Table 41: Fitting left/fitting right (indicates which fitting is used on both ends of the capillary) Description Swagelok + 0.8 mm Port id Swagelok + 1.6 mm Port id Metric M4 + 0.8 mm Port id Metric M3 + 1.6 mm Port id Swagelok union Long...
Page 271: Waste Electrical And Electronic Equipment (Weee) Directive
Appendix Waste Electrical and Electronic Equipment (WEEE) Directive ag-textnode-version-id: 3002258059 ag-textnode-base-id: 3002258059 Waste Electrical and Electronic Equipment (WEEE) Directive This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste.
Page 272: Radio Interference
Radio Interference ag-textnode-version-id: 3002261771 ag-textnode-base-id: 3002261771 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
Page 273: Rfid Statement
Appendix RFID Statement ag-textnode-version-id: 18014403738255755 ag-textnode-base-id: 5228773771 RFID Statement Brasil Este equipamento não tem direito à proteção contra interferência prejudicial e não pode causar interferência em sistemas devidamente autorizados. Para mais informações, consulte o site da Anatel: https://www.gov.br/anatel/pt-br. Este produto não é apropriado para uso em ambientes domésticos, pois poderá causar interferências eletromagnéticas que obrigam o usuário a tomar medidas necessárias para minimizar estas interferências.
Page 274 Appendix RFID Statement Thailand เครื � อ งโทรคมนาคมและอุ ป กรณ์ น ี � ม ี ค วามสอดคล้ อ งตามมาตรฐานหรื อ ข้ อ กํ า หนดทางเทคนิ ค ของ กสทช. This telecommuinication equipment conforms to NTC/NBTC technical requirement. 1. User Information according to FCC 15.21:Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
Page 275: Sound Emission
Appendix Sound Emission ag-textnode-version-id: 3002271243 ag-textnode-base-id: 3002271243 Sound Emission Sound Pressure Sound pressure Lp < 70 db(A) according to DIN EN ISO 7779 Schalldruckpegel Schalldruckpegel Lp < 70 db(A) nach DIN EN ISO 7779 Diode Array Detectors User Manual...
Page 276: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet ag-textnode-version-id: 3002266251 ag-textnode-base-id: 3002266251 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: https://www.agilent.com Diode Array Detectors User Manual...
Page 277 The manual describes the following: • introduction and specifications, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • hardware information, • safety and related information. www.agilent.com © Agilent Technologies Inc. 2014-2024 Edition: 10/2024 Document No: SD-29000132 Rev. D...

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