Related Manuals for Rocker Tanfil 100
Summary of Contents for Rocker Tanfil 100
- Page 1 INSTRUCTION MANUAL Tangential Flow Filtration System Model No. Tanfil 100 Please read this instruction manual before using this product.
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Page 3: Table Of Contents
Table of Contents Introduction Important Notice Unpacking Product Description (1) Tanfil 100, TFF System (2) Operation Panel (3) Parts Material List Button / Symbol / Display Explication (1) Buttons (2) Symbols (3) Display Installation (1) Main Part 1.1 Support Rod 1.2 Gauge Mounting Set... - Page 4 Operation (1) Important Notice Before Starting (2) Tubing Break-in Procedure (3) Cleaning and Flushing (4) Sample Concentration, Purification, or Diafiltration 4.1 Diafiltration (5) Draining 5.1 Drain by Gravity 5.2 Drain by Pump Maintenance Troubleshooting...
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Page 5: Introduction
1. Introduction Tanfil 100 is a laboratory scale tangential flow filtration (TFF) system designed for performing concentration, purification and diafiltration of biomolecules, including proteins, peptides, and nucleic acids. This user-friendly system incorporates a peristaltic pump, 500 mL liquid reservoir, 2 pressure gauges, and magnetic stirrer, creating a simple and ready to use system. -
Page 6: Important Notice
2. Important Notice This instrument is designed for laboratory usage only. Please read this manual carefully before installing and operating. The instrument shall not be modified in any way. Any modification will void the warranty and may result in potential hazard. We are not responsible for any injury or damage caused by any non-intended purposes and modifying the instrument without authorization. - Page 7 Do NOT start the system with the pump head open. Pressure in tubing should be less than 40 psi, or the tubing would be expanded due to overpressure. Please turn the instrument off and disconnect it from the main power before replacing the fuse.
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Page 8: Unpacking
If there is any problem, please keep the serial number along with packing case and contact your local distributor immediately for assistance. Model Standard Package Includes: Tanfil 100, TFF System (Main Unit) Support Rod Feed Gauge Mounting Set Retentate Gauge Mounting Set Capsule Bracket Set... - Page 9 The following items are packed in Fittings / Parts Bag: Three-Way Stopcock *4 Screw Clamp 2 m Tube-ULTR-C *1 for Tubing *1 (translucent) Male Luer Coupler *2 Female Luer Lock 0.5 m Tube-Pharmed BPT *1 - 1/8” hose barb *2 (opaque) Male Luer Rotating Lock Male Luer Rotating Lock...
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Page 10: Product Description
4. Product Description (1). Tanfil 100, TFF System Position Designation Position Designation Main Unit Retentate Gauge Mounting Set Operation Panel Capsule Bracket Set Pump Head Capsule / Cartridge (not included) Magnetic Stirrer Power Switch Feed Gauge Mounting Set AC Socket and Fuse Holder (Input) -
Page 11: Parts Material List
(3). Parts Material List Part Material Part Material Reservoir Gauge Wetted Parts SS316 Reservoir Lid Gauge Mounting Block Reservoir O-ring Silicon Luer Fittings SS316, PP Magnetic Stir Bar PTFE Coated Luer Fitting O-ring Silicone Three-Way Stopcock PC, POM Tubing - ULTR-C Platinum-Cured Silicone Tubing - Pharmed BPT TPE*... -
Page 12: Symbols
(2). Symbols Symbols Designation Description ↑ ↓ Type of Timer Counter / Countdown Timer Pump Head Direction CW / CCW Run at Maximum speed (600 rpm) with pump head Fast Clean Mode direction (CW / CCW) (3). Display Running Mode Fast Clean Mode Position Designation... -
Page 13: Installation
6. Installation (1). Main Part 1.1 Support Rod (A) Insert the support rod on the specified hole, ensuring the flat side of the rod faces the back side of main unit. (B) Use provided hex wrench (2.5 mm) to loosen the screw located in the back plate of main unit. - Page 14 Retentate Gauge Mounting Set Capsule Bracket Set Feed Gauge Mounting Set (C) Ensure the gauge faces forward, then install the feed gauge mounting set by aligning the pin with the hole on the case and securing it by fastening the thumb screw. (D) Install the capsule bracket set through the support rod first and secure it by fastening the thumb screw.
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Page 15: Magnetic Stirrer And Reservoir
1.3 Magnetic Stirrer and Reservoir Installed Rods and Spring Plates Reservoir Magnetic Stirrer AC Socket for Magnetic Stirrer (A) Position the magnetic stirrer into the left compartment of the main unit, and plug its adaptor into the AC socket at the back of the main unit labeled “Max. 5W”. (B) Set the reservoir on the magnetic stirrer with outlet toward left side of the main unit. - Page 16 Pump Tube Rententate Tube Male Male Tubing Clamp Male Male Concentrate Tube Male Male (elbow) Male: Male Luer Rotating Lock - 1/8” hose barb Male (elbow): Male Luer Rotating Lock - 1/8” elbow hose barb Install a tubing clamp over each tubing connected to the hose barb. Tighten clamp by pinching it.
- Page 17 ULTR-C tubing ULTR-C tubing Pump Tube (Pharmed BPT tubing) System Without Capsule Retentate Tube Concentrate Tube (ULTR-C tubing) (ULTR-C tubing) Pump Tube (Pharmed BPT tubing) System With Capsule...
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Page 18: Capsules (Or Cartridges)
(2). Capsules (or Cartridges) Example of connecting capsule and cartridge Left: Pall Minimate Capsule, Right: HansaBioMed TFF Cartridge (A) Remove the caps from both the feed and retentate ports of capsule. Please keep all caps for storage of capsule or cartridge. (B) Connect the feed port of capsule to the side of feed gauge mounting set using the provided luer fittings (and tubing). -
Page 19: Typical Set Up Of Tff System
(F) Adjust capsule bracket set to appropriate position to hold the capsule. The rings on bracket can be adjusted by loosening screw with a hex wrench (2.5mm). (3). Typical Set up of TFF System Pressure Gauges Capsule / Cartridge Reservoir Filtrate Sample... -
Page 20: Operation
7. Operation (1). Important Notice Before Starting There are several procedures during operation, please follow the instruction for system set up. The recommended operational details and parameters may vary based on the specific capsules and cartridges; therefore, refer to the manufacture’s instructions for proper handling. ◆... -
Page 21: Tubing Break-In Procedure
Please ensure to break the tubing in before running the sample. (A) Install the Tanfil 100 main unit and tubing as instructed in Chapter 6. Installation, without attaching a capsule or cartridge. The system setup for break-in procedure is shown above. -
Page 22: Cleaning And Flushing
Capsule Cleaning System Cleaning (A) Install the Tanfil 100 main unit and tubing as instructed in Chapter 6. Installation, and adjust the three-way stopcocks as shown above. (B) Fill the reservoir with 250 mL of purified and filtered water and recirculate for 15 minutes. -
Page 23: Sample Concentration, Purification, Or Diafiltration
(4). Sample Concentration, Purification, or Diafiltration Repeat the setup and instructions on p.18 (3) Cleaning and Flushing, but substitute the sample liquid for purified water or cleaning solution. ◆ A screw clamp can be clamped on concentrate tube to adjust the pressure and filtrate flow. ◆... -
Page 24: Draining
(5). Draining Draining is used to remove the liquid from the system, such as cleaning solution or sample liquid. Therefore, it always follows the cleaning, concentration, or diafiltration procedure. Two different setups for draining are available: by gravity or by pump. Follow the setup outlined in this section for the desired method. -
Page 25: Drain By Pump
5.2 Drain by Pump (A) Attach a hose barb fitting (Male Luer Lock - 1/8” hose barb) to the three-way stopcocks at the front of feed gauge mounting set. (B) Connect a ULTR-C tubing (translucent) to hose barb and put the other end of tubing in a vessel. -
Page 26: Troubleshooting
9. Troubleshooting Problem Reason and Solution Loose plug Reconnect plug to power supply. Abnormal Burnt fuse Replace with a new fuse. Display Display or components failure Contact distributor for assistance. Pump module overload Slow down speed, release screw clamp, Motor Shutdown then restart the instrument. - Page 27 10. Ordering Information 184100-01(02) Tanfil 100, Tangential Flow Filtration System, AC100-240V, 50/60Hz, US plug (EU plug) 203100-01(02) MS 100, Low Profile Magnetic Stirrer, AC100-240V, 50/60Hz, US plug (EU plug) 203100-10 PTFE Magnetic Stirring Bar, Ø 3.6 x 25 mm 184100-40...
- Page 28 Rocker Scientific Co., Ltd. Tel: +886-2-26033311 Fax: +886-2-26036622 E-mail: export@rocker.com.tw https://www.rocker.com.tw...
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