1. Manuals
  2. Brands
  3. Berthold Manuals
  4. Measuring Instruments
  5. LB 9510
  6. Operating manual

Berthold Lumat LB 9510 Operating Manual

Luminescence tube luminometer
Hide thumbs Also See for Lumat LB 9510:
Table of Contents

Advertisement

Quick Links

Download this manual
LB 9510
Lumat Luminescence Tube Luminometer
Operating Manual
71450BA2
Rev. No.: 02, 10/2023

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Lumat LB 9510 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Berthold Lumat LB 9510

Summary of Contents for Berthold Lumat LB 9510

  • Page 1 LB 9510 Lumat Luminescence Tube Luminometer Operating Manual 71450BA2 Rev. No.: 02, 10/2023...
  • Page 3 The information in this guide is subject to change without notice. DISCLAIMER TO THE EXTENT ALLOWED BY LAW, BERTHOLD TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

  • Page 5: Table Of Contents

    LB 9510 Lumat Table of Contents Table of Contents Prefatory Comments ................7 Contact Information .................. 7 Typographical Conventions ............... 8 Safety Manual and Operating Manual ............9 1.3.1 The Operating Manual ................9 Software Installation ................ 11 Installation of LightCompass®® Software ..........11 Software Registration ................
  • Page 6 Table of Contents LB 9510 Lumat Quality Control Check ............... 31 Warranty and Technical Issues ............32 Special Spare Parts .................. 32 Warranty Statement ................32 Customer Service ..................33 Technical Data of LightCompass® Software ........34 Index ....................35 Page 6 of 35 71450BA2 Rev.

  • Page 7: Prefatory Comments

    LB 9510 Lumat 1 Prefatory Comments Prefatory Comments Contact Information Berthold Technologies GmbH & Co. KG Calmbacher Str. 22 75323 Bad Wildbad Germany Sales and Customer Service Internet: www.berthold.com Phone: +49 7081 177-0 Fax: +49 7081 177-100 E-Mail: bio@berthold.com Central Customer Service...

  • Page 8: Typographical Conventions

    1 Prefatory Comments LB 9510 Lumat Typographical Conventions j DANGER Indicates an imminent, major hazard, which will certainly result in seri- ous injuries or even death if the hazard is not avoided. j CAUTION Refers to a potentially dangerous situation, which can result in me- dium or minor physical injuries or damages to property, if it is not avoided.

  • Page 9: Safety Manual And Operating Manual

    1.3.1 The Operating Manual The operating manual is valid from the delivery of the Berthold product to the user until its disposal. Keep the operating manual for future refer- ence. Read all instructions thoroughly and completely before working with the product.
  • Page 10 1 Prefatory Comments LB 9510 Lumat 10/2023 Added LightCompass® Trademark Page 10 of 35 71450BA2 Rev. 02, 10/2023...

  • Page 11: Software Installation

    LB 9510 Lumat 2 Software Installation Software Installation The instrument is operated with LightCompass® software. Follow the in- structions for LightCompass® software installation. IMPORTANT Precondition for both software packages: Windows compatible PC with Windows 10. Installation of LightCompass® Software IMPORTANT Technical Data of LightCompass®...

  • Page 12: Software Registration

    Obtain Password: Press the Web button on the registration form, in- sert the required information in the web registration form and forward it to Berthold Technologies. Page 12 of 35 71450BA2 Rev. 02, 10/2023...
  • Page 13 Please provide the System ID of the PC. The number is printed in red in the registration dialog of the software (see picture above). The owner will receive the registration password from Berthold Tech- nologies via E-Mail. Enter Password: Enter the password within 30 days to enable unlim- ited use.

  • Page 14: Lightcompass® Software

    3 LightCompass® Software LB 9510 Lumat LightCompass® Software LightCompass® Directories and Files The directories for data and parameter files are defaulted as described below. Any accessible directory on the computer and the local network can be selected though when saving data and parameter files using the “Save …...

  • Page 15: Lightcompass® User Interface

    3.2.2 Instrument Menu In the Instrument menu basic instrument settings and communication may be accessed. [Detect Instrument] detects the connected Berthold instrument. [Load Tube] turns sample rotor once: moves tube from loading position into measurement position. [Prime] Opens the injector priming sequence (filling the lines).

  • Page 16: Settings Menu

    [Configuration of Instrument]: To check the configuration of your instrument, click [Settings] / [Driver] / [Berthold Lumat] / <Get Instrument Configuration>. In the configura- tion submenu you may search the instrument, activate the calculation of RLU/s for your measurement data instead of RLU, get the configuration of the connected instrument and calculate the correction factor for the sample adapters.

  • Page 17: Help Menu

    LB 9510 Lumat 3 LightCompass® Software usually very similar. The adapters are matched and paired at the factory. For extremely precise measurements or in case one of the adapters got dirty you can adjust both responses by determining the RLU correction factor.

  • Page 18: Operation With Lightcompass

    4 Operation with LightCompass® LB 9510 Lumat Operation with LightCompass® Defining protocols using the Protocol Wizard of LightCompass® software and running measurements on the Lumat is straight forward. The proce- dure is the same for Raw Data, Curve Fit and Cut-off. In order to provide the utmost light collecting efficiency the tube holders are equipped with reflectors which may exhibit phosphorescence after exposure to light.

  • Page 19: Define A Protocol Using The Protocol Wizard

    LB 9510 Lumat 4 Operation with LightCompass® Define a Protocol using the Protocol Wizard IMPORTANT Protocol wizard The protocol setup can be guided through a wizard. Most of the steps passed through the protocol setup are similar in all kinds of protocols, Raw Data, Cut-Off and Curve Fit.

  • Page 20: Kinetic

    4 Operation with LightCompass® LB 9510 Lumat [Measurement Settings] Define the measurement time (counting time) for each tube. 4.2.3 Kinetic [Label Settings] Here you can define a name for this specific step and give a description. [Kinetic Settings] Here you can set up the total time the measurement should last, the counting time for each measurement step and an optional delay time.

  • Page 21: Protocol Wizard - Protocol Type And Properties Pages

    LB 9510 Lumat 4 Operation with LightCompass® Protocol Wizard - Protocol Type and Properties Pages Select the type of protocol evaluation on the Protocol Type Page. Depending on your selection – Raw Data / Cut-Off / Curve Fit protocol – the following different Properties Pages will be available.
  • Page 22 4 Operation with LightCompass® LB 9510 Lumat Specific submenus and check boxes may be available or greyed out, de- pending on your selected protocol settings. Select the [No. of Standards] Minimum is 4 standards. [No. of Controls] Select 0-10 controls. [No.

  • Page 23: Protocol Wizard - Export Page And Print Page

    LB 9510 Lumat 4 Operation with LightCompass® and the Axis Scaling between Lin/Log and Log/Log For many research assays utilizing a standard curve, a log/log transfor- mation and linear regression are often used. [Concentration Settings] Insert the concentration units and, in case of defined calibrators, insert the calibrator concentration.

  • Page 24: Protocol Wizard - Protocol Page

    4 Operation with LightCompass® LB 9510 Lumat Define your settings for printout in the Print Page. Protocol Wizard - Protocol Page Enter an identifier (File Name) for the protocol under which it is to be saved. Perform a Measurement NOTICE Use of injectors: If injectors are to be used, they must be primed prior to protocol start.
  • Page 25 LB 9510 Lumat 4 Operation with LightCompass® usually Sample and the required Injectors, one after the other to your defined rack size by clicking <#> for assignment to all samples, or into the respective lines/wells in case of assignment to single samples. This assignment will activate the <Start>...

  • Page 26: Maintenance

    For good operation, the injector lines have to be maintained regularly and must be primed previously and washed after use. • Berthold Technology recommends strongly to perform the priming with deionized water first and leaving the lines filled with deionized...

  • Page 27: Customizing The Priming Sequence

    LB 9510 Lumat 5 Maintenance Select the [Injectors] to be primed by checking the check box. All in- jectors may be selected at the same time. Check [Customized Prime Settings] to select a user defined priming method. See chapter 5.1.2 for further information. The default prime instruction is used if [Customized Prime Settings] is unchecked.
  • Page 28 5 Maintenance LB 9510 Lumat The [Prime Customize] dialog will be displayed. Click [Add] and select your settings. Available settings: Injection: the injector is filled with the max. injection volume from the reagent reservoir and injects the set volume Volume percentage of the max.

  • Page 29: Empty The Tubing

    LB 9510 Lumat 5 Maintenance Delay delay before the operation in sec Speed 1 … 10 Repeats number of repeats Select a setting and click [Edit] to change the settings. Confirm with <OK>. Setup a Prime Step Sequence by adding all necessary settings. [Re- move], [Move Up], [Move Down] or [Edit] every step, or [Remove all] steps.

  • Page 30: Further Maintenance Information

    Enter the [Number of Wash Cycles]. One wash cycle consists of one shot. 10 cycles ensure that the injector system is completely filled. Depending on the substances used, Berthold Technologies recom- mends up to 30 cycles for washing a single 100µl injector and up to 10 cycles for 300µl injectors (repeat with a new tube, if necessary).

  • Page 31: Quality Control Check

    It is recommended to run known internal quality standards or samples attendant to the measurement runs or to use the Luminescence Test Tube, offered by Berthold Technologies. 71450BA2 Rev. 02, 10/2023 Page 31 of 35...

  • Page 32: Warranty And Technical Issues

    • the product has not been regularly and correctly maintained • the product has not been repaired or modified by a Berthold Tech- nologies authorized representative or user • parts other than original Berthold Technologies parts are used •...

  • Page 33: Customer Service

    Customer Service Customer service will be provided in the first instance by the network of Berthold Technologies representatives. In the event of any problem ex- perienced with your instrument, the first recourse should be your local Berthold Technologies representative. For further problems requiring hardware or software expertise, the Technical Support group at Berthold Technologies GmbH &...

  • Page 34: Technical Data Of Lightcompass® Software

    8 Technical Data of LightCompass® Software LB 9510 Lumat Technical Data of LightCompass® Software Operating system Win 10 (32/64bit) PC requirements Pentium like CPU (2 GHz or better/intel Core iX recommended), 1 free USB 2.0 port. Interface(s) LightCompass® Soft- Wizard support for parameter entries ware Selection of wells Endpoint...

  • Page 35: Index

    LB 9510 Lumat 9 Index Index Cleaning the Tubings ......28 LightCompass® Software Installation 10 Confirmation on Decontamination ..32 LightCompass® Technical Data ..33 Contact Information ......7 LightCompass® User Interface ..14 Curve Fit ..........20 Luminescence Test Tube ....30 Customer Service .......

Table of Contents