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CoolLED pE-800 Series Quick Start Manual

Sequence runner in nikon nis elements
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Table of Contents

Quick Start Guide for Sequence Runner in
Contents
1
What is Sequence Runner? ............................................................................................ 1
2
Equipment Required ..................................................................................................... 1
3
Hardware setup ............................................................................................................ 2
4
Configuring Sequencer Mode in NIS Elements ................................................................ 4
5
How Fast is Sequence Runner? ...................................................................................... 9
6
Useful Documents ......................................................................................................... 9

1 What is Sequence Runner?

Sequence Runner provides a cost-effective approach for high-speed multi-colour fluorescence
imaging. Using the Global TTL input of the CoolLED Illumination System, Sequence Runner allows a
camera's TTL output to drive a user-defined LED sequence. LED channel selection and intensity is
configured in NIS Elements, and each LED in the sequence is triggered on receiving a TTL signal. This
simple approach provides high-speed imaging with illumination precisely synchronised to the
camera, which also minimises phototoxicity and photobleaching.

2 Equipment Required

In addition to a fluorescence microscope setup, the following components are required:
•
NIS Elements AR
•
Illumination Sequence NIS module
•
Triggered Device Control NIS module
•
A CoolLED Illumination System which is supported in NIS Elements and features the
Sequence Runner Function. These are currently:
o pE-800
o pE-800
fura
o pE-300
ultra
o pE-340
fura
•
Camera with TTL output
•
Compatible cable to connect camera TTL output to Global TTL (BNC) of CoolLED Light Source
Nikon NIS Elements
IQS015- Rev 01
Issue Date: 30/10/2023
1

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Summary of Contents for CoolLED pE-800 Series

  • Page 1: Table Of Contents

    Sequence Runner provides a cost-effective approach for high-speed multi-colour fluorescence imaging. Using the Global TTL input of the CoolLED Illumination System, Sequence Runner allows a camera’s TTL output to drive a user-defined LED sequence. LED channel selection and intensity is configured in NIS Elements, and each LED in the sequence is triggered on receiving a TTL signal.

  • Page 2: Hardware Setup

    • For the pE-800 Series, remove the filter holder using the thumbscrews and insert filters with the arrow on the filter facing down. It is important to secure all filters using the grub screw and hex key (provided with purchase of Illumination System).
  • Page 3 2. To allow the LED sequence to be configured in NIS Elements, connect the CoolLED Illumination System to the PC via USB (Figure 3). The USB cable is supplied with purchase of CoolLED Illumination System. Figure 3: Connect the Illumination System to the PC via USB cable 3.

  • Page 4: Configuring Sequencer Mode In Nis Elements

    3. To enter Sequencer (Sequence Runner) mode, scroll down the pE-800 Pad and click ‘Configure…’ (Figure 6). Note: In this example, NIS Elements is already configured for our hardware setup. If the CoolLED Pad shown is not showing, please see Quick Start Guide or...
  • Page 5 Figure 6: Click 'Configure...' (red box) to open the pE-800 Configuration window 4. When the pE-800 Configuration window opens, from the ‘Connection’ menu, select ‘USB + Global BNC (Sequencer.)’ (Figure 7) and close the Configuration window. Figure 7: Enter Sequencer (Sequence Runner) mode by selecting USB + Global BNC (Sequencer) 5.
  • Page 6 6. Figure 9 shows the configuration options available. Firstly, add the LEDs you wish to appear in the sequence by clicking the blue ‘+’ icon. The number under the ‘#’ column indicates this LED’s position in the sequence. To change the selected LED, use the dropdown menu under ‘Line’. 7.
  • Page 7 Figure 10: LED intensity can also be adjusted on the pE-Pad using the sliders or entering percentage values. Left: while configuring Sequence Runner (it is not necessary to select LEDs); Right: when Sequence Runner is active, intensity can be adjusted in real time 11.
  • Page 8 4.1 How to further increase speed 1. Reducing the ROI enables even faster imaging speeds, while also minimising the area that can potentially become photobleached. On the camera control window, select the ‘ROI’ checkbox and click ‘ROI Size’. This opens the dropdown menu, where an ROI size can be selected or defined.

  • Page 9: How Fast Is Sequence Runner

    Sequence Runner completed acquisition in 4.8 seconds 6 Useful Documents NIS Elements Quick Start Guide NIS Elements tutorial video pE-800 Series user manual pE-300 Series user manual fura pE-340 user manual For further information or questions regarding CoolLED Illumination Systems, please contact info@coolled.com.

This manual is also suitable for:

Pe-800Pe-800furaPe-300ultraPe-340fura

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