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CoolLED pE-340fura User Manual

Illumination system
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User Manual

fura
pE-340
DOC-034 Iss 3
1

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Summary of Contents for CoolLED pE-340fura

  • Page 1: User Manual

    User Manual fura pE-340 DOC-034 Iss 3...

  • Page 2: Table Of Contents

    Table of Contents Introduction ........................3 Safety Precautions ......................4 Getting Started – System Components ................6 Installation and setup ...................... 7 Configuration of the Light Source ................... 9 Operation - Manual Control ..................10 Remote Operation – TTL ....................13 Remote Operation –...

  • Page 3: Introduction

    Introduction fura CoolLED’s pE-340 Illumination System has been designed to meet the requirements of users carrying out Fura-2 AM ratiometric calcium imaging. fura The pE-340 offers an Illumination System that is both safer and offers a faster level of control than traditional bulb-based solutions. The three controllable channels offer 340nm, 380nm and a broad ‘white’...

  • Page 4: Safety Precautions

    Safety Precautions While LEDs are a much safer illumination system than the mercury and metal halide lamps that they replace in microscopy applications, precautions should still be taken with this product. When operating or maintaining this product, please observe the following safety precautions at all times.
  • Page 5 2.7. To clean the exterior of the Light Source, use a slightly dampened cloth with a simple water/detergent solution only. Avoid the optical surfaces and lenses. Cleaning of optics should only be carried out using optical wipes and fluids. Please note that the DC power supply unit should be isolated prior to cleaning.

  • Page 6: Getting Started - System Components

    3. Microscope adaptor for specific microscope model (Direct fit only). 4. DC Power Supply Type GST120A12-R7B. 5. IEC Power Cable (not shown). User Guide (not shown). If any components are missing or appear damaged, please contact CoolLED immediately. DOC-034 Iss 3...

  • Page 7: Installation And Setup

    DC power supply is the one supplied with the product. Using non- CoolLED power supplies may damage the Light Source and will invalidate the warranty. At this stage do not connect the mains power lead to the DC power supply.
  • Page 8 4.4. Attach the LED Light Source to the epi-fluorescence port on your fura microscope. Your pE-340 Light Source will have been supplied with a compatible fitting for the microscope you specified at order (if direct fit version). Attach the Light Source ensuring that it is secure and true/flush with the microscope.

  • Page 9: Configuration Of The Light Source

    Configuration of the Light Source 5.1. 5.2. fura The pE-340 has independent circuits giving the user control of the three main peaks of emissions. These are referred to as 340 (nm), 380 (nm) and 3WT (white illumination). DOC-034 Iss 3...

  • Page 10: Operation - Manual Control

    Operation - Manual Control 6.1.1. Manual Control Pod Operation on/off. fura The pE-340 is easily controlled from the manual Control Pod. LEDs are switched on and off by pressing the ‘on/off’ button. 6.1.2. At start-up the Light Source will revert to the same settings that were set when it was last powered down.
  • Page 11 6.1.4. To switch off the LEDs, press the ‘on/off’ once again. 6.1.5. Intensity Control. The Control Pod enables the user to control the intensity of the LEDs that are exciting different stains. This helps to balance the emissions so that one stain does not dominate another.
  • Page 12 6.1.7. Individual channels can be switched off (de-selected) by pressing the ‘select’ button. Light is then only generated where it is required to excite the stains in use. This has many attractive benefits with improvements in contrast, cell viability and savings in energy. 6.1.8.

  • Page 13: Remote Operation - Ttl

    Remote Operation – TTL 7.1. Global triggering 7.1.1. fura The pE-340 has a BNC socket on the rear of the Light Source which allows global control of the Illumination System Global BNC socket The TTL signal controls the on/off function of the Light Source. A TTL ‘high’ will cause the LEDs to be on, independent of the state of the on/off button.
  • Page 14 7.2.2. The TTL signal controls the on/off function of the Light Source. A TTL ‘high’ will cause the LEDs to be on. The channel controls shall trigger the corresponding channel regardless of its on/off state or whether it has been selected using the Control Pod.
  • Page 15 7.3.3. Order in triggering sequence ON/OFF state of the Illumination System. Channel intensity (0-100%) Installed channels 7.3.4. Pressing the channel select button allows you to either deselect a channel or change the order that it triggers in the sequence. DOC-034 Iss 3...
  • Page 16 7.3.5. Pressing the + & - buttons on the Control Pod will allow the light intensity of the corresponding channel to be increased or decreased. 7.3.6. The sequence will not begin until the ON/OFF button on the Control Pod is pressed.
  • Page 17 7.3.8. An example of the TTL signal is shown below with labels showing the effect on the light output during the sequence. channel channel channel channel of sequence of sequence of sequence of sequence 7.4. TTL triggering information 7.4.1. The TTL input circuit has been designed to maximise the switching speed of the LEDs to give the user precise control of the excitation light reaching the sample.

  • Page 18: Remote Operation - Usb

    8.3. When you first plug your CoolLED system into your PC with the USB cable, Windows will ask for a driver file unless one has already been installed. You should point Windows to the file available from CoolLED.
  • Page 19 CoolLED website: http://www.coolled.com/product-detail/imaging-software/ 8.5. Click on the link at the bottom of the Overview section titled ‘CoolLED pE_Driver’. Download then unzip before pointing Windows to this file. 8.6. Once the CoolLED device has been successfully installed into Windows you should look at the Virtual COM ports assigned by going into Device Manager.

  • Page 20: Optical Setup

    Optical setup 9.1. Direct fit version 9.1.1. fura The pE-340 has been designed to work on the majority of fluorescence microscopes, both new and old. As would be expected, there is some variation in the optical path and elements within every microscope. In order fura to accommodate these variations, the pE-340 is supplied with a small...
  • Page 21 Illumination System is also available with a liquid light guide output. The Illumination System is designed to accept a 3mm core light guide. CoolLED offer a liquid light guide that has been selected to offer maximal transmission of the 340nm channel. It should be noted that if an alternative light guide is used you may note a reduction in optical power when using the 340nm channel.
  • Page 22 9.2.3. fura The pE-340 Illumination Systems with liquid light guide output are provided with a ‘cradle’ to ensure that they remain in a stable position during operation, as shown in the image below. 9.2.4. The use of a liquid light guide will be attractive, as this allows the light source to be placed outside the Faraday cage to reduce vibration and fura electrical noise close up to the samples.

  • Page 23: Additional Filtering Capability

    9.2.5. fura The pE-340 Universal Collimator has been designed with optics to allow maximal transmission of the 340nm channel. When using this collimator, it is important to set up the optics correctly to optimise the performance of the Illumination System. Full setup instructions are given in the separate User fura Manual for the pE-340 Universal Collimator.

  • Page 24: Excitation Filter Specifications

    10.3. Due to the shape of the excitation filter holder slide, it can only be fitted into the corresponding channel in one orientation. 10.4. To install excitation filters in the optimal orientation, the direction of light through the Light Source should be observed. This is shown in the image below with arrows.
  • Page 25 11.2. Transmission spectrum DOC-034 Iss 3...

  • Page 26: Settings / Additional Information

    Settings / Additional Information 12.1. Display Backlight and Contrast settings The Control Pod display settings can be adjusted to suit the lighting environment that the instrument is being operated in. To make adjustments, press and hold the ‘mode’ button for 3 seconds. Use the first column up/down buttons to adjust the backlight to the desired level.
  • Page 27 12.2. System Information To interrogate the product on its hardware and firmware revisions, press and hold the ‘mode’ button for 3 seconds. Once the display settings screen appears as in 11.1, release the ‘mode’ button and then give it a second short duration press.

  • Page 28: Routine Care And Maintenance

    Every microscope manufacturer has one or a number of methods of attaching the fluorescence light source. CoolLED has designed a comprehensive range of adaptors to match these microscopes.
  • Page 29 14.4. Fit the new adaptor and tighten grub screws. 14.5. A complete list of adaptors can be found on the CoolLED website using the link: www.coolled.com/product-detail/adaptors-2/ 14.6. The simple optical setup procedure will need to be followed when fitting the...

  • Page 30: Product Specifications

    167mm (w) x 67mm (d) x 35mm (h) -weight 0.62kg 15.4. Environmental Operating Conditions Operating 5 – 35°C Product options and order codes See website (Microscope Illuminators | LED Illumination Systems | CoolLED) for full details of product options and order codes. DOC-034 Iss 3...

  • Page 31: Warranty And Repairs

    Council of 8 June 2011 on the restriction of the use of certain hazardous substances in electrical and electronic equipment (also known as “RoHS”). This declaration is correct to the best of CoolLED Ltd knowledge, information and belief at the date of its release. DOC-034 Iss 3...

  • Page 32: Contact Details

    End-of-Life Light Sources. You can help us by filling in our online contact form and providing us with your contact details and the serial number of the CoolLED Light Source that you wish to return and we will collect it free of charge.

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