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8.
If using a laboratory stand, mount the converter/probe assembly using a clamp. Be
sure to secure the clamp to the upper section of the convertor housing only (see
page 10). Do not secure the clamp to any other portion of the convertor/probe
assembly.
9.
Connect the converter cable to the power supply.
7.3. OPERATION
Continuous mode:
1.
Ensure that the amplitude control knob is set to 0.
2.
Set the converter and probe assembly in a stand or hold the convertor in your hand.
3.
Turn the unit on.
4.
Immerse the probe tip half way into the sample. If the probe is immersed to an
insufficient depth, air will be injected into the sample, causing the sample to foam.
Also ensure that the probe tip is not touching the wall of the sample vessel as it
may be damaged and it will not vibrate properly.
5.
Turn the amplitude setting dial to the intensity that you wish to run the unit at.
6.
Sonicate sample for the desired amount of time and then turn the amplitude set-
ting dial back to 0.
Remote (Thumb switch or Footswitch) mode:
1.
Ensure that the amplitude control knob is set to 0.
2.
Set the switch on the front panel to thumb switch.
3.
If needed, connect the foot switch cable to the designated jack on the back of the
power supply. Place the footswitch in a convenient location on the floor in front
of the unit.
4.
Hold the convertor in your hand or set the convertor and probe assembly in a stand.
5.
Turn the unit on.
6.
Turn the amplitude setting dial to the intensity that you wish to run the unit at.
7.
Place the probe tip into the liquid sample being sonicated. Ensure that the probe
tip is not touching the wall of the sample vessel and that it is properly submerged
in the sample liquid to prevent aerosolization/foaming.
8.
Depress the thumb switch or footswitch to start sonication of the sample.
9.
Sonicate for the desired amount of time and then release the thumb switch or
footswitch to stop sonication.
Note: sonication will generate heat and pulsed sonication is recommended to pre-
vent overheating of both the sample and converter. Chilling the sample using an ice
bath or other appropriate method is recommended.
Continuous sonication should be limited to 1 minute. Longer sonication time
should be done in a pulse mode.
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