Human HumaFIA User Manual page 34

Semi-automated time-resolved fluorescence immunoassay analyser
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3 ROUTINE UTILIZATION AND MEASUREMENT
Follow these instructions for each selected control material to run controls in Fast mode:
In the Fast mode worklist in the column Timer, the button Next Con. appears. The number
of seconds indicates when the first (or next) control material can be started in Fast mode.
If the number of seconds equals 00, testing can be started. In this case, the button 80µl
vol. added is shown.
The next step is to add 80 µl of the control to the cartridge. Once this is done, press this
button immediately. This starts the monitoring of the incubation time for this control
reading. For details, see IFU.
For this control, the remaining incubation time is now shown in the format mm:ss. Once the
incubation time for this control is almost up, the Read button appears.
Now there are 10 seconds left to insert the cartridge into the analyser and press the Read
button. For optimal results, press the Read button when the timer equals 00. Reading takes
only a few seconds, then the result of this control parameter is displayed.
If the timing was correct, --:-- appears, indicating that the incubation time was correct, or
in the acceptable range.
If a number in red appears, like +02:11, the incubation time was 2 minutes and 11 seconds
too long, indicating that the result is not valid and that the test must be repeated.
To run a series of control materials, start the workflow described above for the next control.
Once the incubation time for the first control has started, this button shows up to signal that
the next control in the Fast worklist can be prepared. The workflow programme runs down
a timer allowing the user to prepare the next control. When the timer equals 00, it means
that the user can select this control to be pipetted.
Now for the second control, the control volume of 80 µl must be pipetted into the cartridge.
Once this is done, immediately press this button. Now the monitoring of the incubation
time starts for the second control of the worklist.
The series as described above is processed for the first and all subsequent controls until the Fast worklist is
empty.
Once the Fast worklist is empty, the following information of the control material is displayed in each line:
LOT, date and time of the reading, control low and high target values, control level and the recorded result of
a control parameter.
Note: A red number in the Timer column indicates an incorrect incubation time. The measurement must be
repeated with another cartridge.
HumaFIA | User manual
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