Buchi C-805 Operation Manual
  1. Manuals
  2. Brands
  3. Buchi Manuals
  4. Laboratory Equipment
  5. Pure C-805
  6. Operation manual

Buchi C-805 Operation Manual

Pure chromatography instrument
Hide thumbs Also See for C-805:
Table of Contents

Advertisement

Quick Links

Download this manual
Pure Chromatography Instrument C-805
Operation Manual

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the C-805 and is the answer not in the manual?

Questions and answers

Related Manuals for Buchi C-805

Summary of Contents for Buchi C-805

  • Page 1 Pure Chromatography Instrument C-805 Operation Manual...
  • Page 2 CH-9230 Flawil 1 E-Mail: quality@buchi.com BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experience, especially with respect to structure, illustrations and technical details. This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for competitive purposes, nor made available to third parties.

  • Page 3: Table Of Contents

    Establishing electrical connections .................... 19 Establishing solvent connection...................... 19 Assigning solvent to solvent lines ......................  20 Assembling the waste bottle ...................... 20 Assembling the solvent bottle platform (option).................  21 Assembling the cartridge holder ...................... 22 Operation Manual Pure Chromatography Instrument C-805...
  • Page 4 Sending PDF to USB ...................... 52 Cleaning and servicing ........................ 53 Regular maintenance work ........................  53 Carrying out a data backup........................  54 Calibrating the display ........................ 54 Cleaning the check valve........................ 54 Cleaning the instrument........................ 55 Operation Manual Pure Chromatography Instrument C-805...
  • Page 5 Tools and adapter kits...................... 66 10.1.4 Tubes ........................... 66 10.1.5 Wear parts ........................... 67 10.2 Folder locations .......................... 67 10.3 Finding out separation conditions with the navigator.................  67 10.3.1 Open the navigator ...................... 67 10.3.2 TLC Silica.......................... 68 10.3.3 LC-C18.......................... 69 10.3.4 LC-Transfer.......................... 70 Operation Manual Pure Chromatography Instrument C-805...

  • Page 6: About This Document

    This character draws attention to a requirement that must be met before the instructions below are carried out. This character indicates an instruction that must be carried out by the user. ð This character indicates the result of a correctly carried out instruction. 6/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 7: Trademarks

    1.4 Connected devices In addition to these operating instructions, follow the instructions and specifications in the documentation for the connected devices. Operation Manual Pure Chromatography Instrument C-805 7/72...

  • Page 8: Safety

    Safety-related incidents that occur while using the device should be reported to the manufacturer (quality@buchi.com). BUCHI service technicians Service technicians authorized by BUCHI have attended special training courses and are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair measures.

  • Page 9: Faults During Operation

    Unauthorized modifications can effect safety and lead to accidents. Use only genuine BUCHI accessories, spare parts and consumables. Carry out technical changes only with prior written approval from BUCHI. Only allow changes to be made by BUCHI service technicians. BUCHI accepts no liability for damage, faults and malfunctions resulting from unauthorized modifications.

  • Page 10: Product Description

    Injection of liquid or solid sample — Separation on a cartridge — Identifying the compounds by UV — Collecting the desired fractions Instrument schematic: Solvent 1 Fraction Pump detector collection Sample introduction Solvent 2 10/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 11: Configuration

    3.2 Configuration 3.2.1 Front view Fig. 1: Front view Control panel RFID reader On/Off switch USB Port Cartridge holder Flash pump Fraction collection bay 3.2.2 Rear view NOTE All electrical connections are not limited energy. Operation Manual Pure Chromatography Instrument C-805 11/72...
  • Page 12 Gradient valve (See Chapter 3.2.3 "Connections on gradient valve", page 13 ) Signal connection LAN port (for external air supply) Power supply connection Fuse On/Off master switch USB ports Type plate (See Chapter 3.3 "Type plate", page 13 12/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 13: Connections On Gradient Valve

    Fig. 4: Type Plate Company name and address Instrument name Serial number Input voltage range Frequency Power consumption maximum Year of manufacture Product code Approvals 10 Symbol for "Do not dispose of as household waste" Operation Manual Pure Chromatography Instrument C-805 13/72...

  • Page 14: Scope Of Delivery

    0 - 50 bar Flow rate 0 - 250 mL/min Flow rate accuracy < 2 % Functional principle self-priming Specification 3 pistons radial arranged UV Detector Pure C-805 UV wavelength range 200 - 400 nm 14/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 15: Ambient Conditions

    Powder coated steel 1.4301 Fraction collection bay PMMA / PET Pump head PEEK Pump cover Pump excenter housing Aluminum Tubings Fitting Valve screw fitting Ferrule ETFE Cone ring Radial seal PTFE Pistons Ceramic Operation Manual Pure Chromatography Instrument C-805 15/72...

  • Page 16: Transport And Storage

    Make sure a clean solvent like ethanol or isopropanol is in the pump. Wherever possible, store the device in its original packaging. After storage, check the device, all seals and tubing for damage and replace if necessary. 16/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 17: Lifting The Instrument

    The instrument should be transported by two persons at the same time. Lift the instrument at the points indicated. Lift the instrument – this requires two persons each lifting at one of the points indicated on the bottom of the instrument. Operation Manual Pure Chromatography Instrument C-805 17/72...

  • Page 18: Installation

    The instrument has an earthquake fixing point to protect the device against falling. Fig. 5: Securing against earthquakes Fixing point Tie the lashing mount to a fixed point using strong cord or a wire. 18/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 19: Establishing Electrical Connections

    Attach all solvent lines to the instrument. Connections see Chapter 3.2 "Configuration", page 11 Put the other end of the solvent line into the solvent bottle. Assign the solvent to the solvent lines. See Chapter 5.6 "Assigning solvent to solvent lines", page 20 Operation Manual Pure Chromatography Instrument C-805 19/72...

  • Page 20: Assigning Solvent To Solvent Lines

    Waste bottle on top of the instrument Waste bottle on top of the instrument can cause property damages. Make sure that there is a decline between the connection on the instrument and the waste bottle. 20/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 21: Assembling The Solvent Bottle Platform (Option)

    Do not place the waste bottle an the solvent bottle platform. Turn the solvent bottle platform upside down. Attach the drain line adapter to the solvent bottle platform Attach the drain line to the drain line adapter. Operation Manual Pure Chromatography Instrument C-805 21/72...

  • Page 22: Assembling The Cartridge Holder

    5.9 Assembling the cartridge holder Attach the bracket with screws to the instrument. Assemble the cartridge clamp to the rod. Fix the position of the clamp with the screw. Attach the assembled cartridge clamp to the bracket. 22/72 Operation Manual Pure Chromatography Instrument C-805...
  • Page 23 ð The cartridge clamp is assembled. The cartridge clamp can be opened by pressing simultaneously both parts together. In this position the cartridge can be placed in the clamp. Operation Manual Pure Chromatography Instrument C-805 23/72...

  • Page 24: Operation

    [Run] panel See Chapter 6.1.7 "Run panel", page 31 [Solvent selection] Shows selectable solvents. panel See Chapter 6.1.8 "Solvent selection panel", page 32 [Detector settings] Shows selectable detector options and its panel settings. See Detector selection panel 24/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 25: Enter Value

    The display shows a dialog with an alphanumeric input box. Enter the value. [OK] Tap the button ð The value is saved. ð The dialog box closes. 6.1.3 Menu bar The following menus are available: Operation Manual Pure Chromatography Instrument C-805 25/72...
  • Page 26 [Run in Progress] [Run in Progress] is marked, a run is in progress. [Past Run] [Past Run] is marked up, the instru- ment is in the past run mode. 26/72 Operation Manual Pure Chromatography Instrument C-805...
  • Page 27 Adjust the baseline to zero during a run. [UV Baseline] Select a language. [Language] [Service] BUCHI service technicians only [Help] [About] The display shows instrument details. The display shows the Operation manual. [View Manual] Operation Manual Pure Chromatography Instrument C-805 27/72...

  • Page 28: Conditions Panel

    [Run Length] Enter value According to the current operation en- ter the time for the separation. According to the current operation en- ter the number of column volumes re- quired for the separation. 28/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 29: Collection Panel

    The fraction collection area contains the following settings: Action Option Explanation Fraction collection Select value The following options are available: options [Collect Peaks] [Collect All] [Collect None] [Per-Vial Volume] View / Enter value The following values are changeable: [Peak] [Non-Peaks] Operation Manual Pure Chromatography Instrument C-805 29/72...

  • Page 30: Gradient Panel

    The gradient is held at the current [Gradient hold] solvent ratio. The gradient continues to the original end-point. Function The gradient will be held every time [Auto gradient the signal goes over the set thresh- hold] old. 30/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 31: Run Panel

    Is used to stop the actual operation. [Pause] If the system is paused due to an er- ror, this button will change to yellow. Is used to advance to the next step [Advance] during equilibration. Operation Manual Pure Chromatography Instrument C-805 31/72...

  • Page 32: Solvent Selection Panel

    Select value / Enter Low / High value Enter values for threshold Action disabled: No selection [Threshold Detec- Enter value The threshold defines the value above the fraction collector starts to tion] collect fractions. 32/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 33: Slope Detection Panel

    6.2.1 Selecting a cartridge (Flash mode) NOTE The automatic reading of the cartridge information can only be done with specific BUCHI RFID tagged cartridges. The indication for recognizing the cartridge is a sound. Operation Manual Pure Chromatography Instrument C-805 33/72...
  • Page 34 Navigate to the Conditions selection panel according the navigation path. [Column] Tap the input box next to ð The display shows a menu with selectable cartridges. Select the cartridges you wish to use. 34/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 35: Selecting A Solvent

    Select more solvents for the mobile phase according to your needs. 6.2.3 Editing the gradient The composition of the mobile phase as a function of time can be indicated by entering the gradient. Solvent lines can be used to generate a binary gradient. Operation Manual Pure Chromatography Instrument C-805 35/72...
  • Page 36 ð The display shows the dialog Gradient Table . [Min] Tap the cell for ð The display shows a dialog box with a numeric input box. Enter the time. [OK] Tap the button 36/72 Operation Manual Pure Chromatography Instrument C-805...
  • Page 37 %B, then release. Deleting steps from the Gradient graphic To remove a step, drag the point to the baseline or to any gray area around the graph until a red X is visible, then release. Operation Manual Pure Chromatography Instrument C-805 37/72...

  • Page 38: Editing The Wavelength For The Uv Detector

    Tap the input box next to ð The display shows a dialog box with a numeric input box. Enter a value for the wavelength. [OK] Tap the button ð The dialog box closes. ð The wavelength is saved. 38/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 39: Editing The Fraction Collection Criteria

    The value for the volume is saved. [Non-Peaks] Tap the input box next to ð The display shows a dialog box with a numeric input box. Enter the volume you wish to collect. Operation Manual Pure Chromatography Instrument C-805 39/72...

  • Page 40: Editing The Fraction Collection Time

    Tap the input box. ð The display shows a dialog box with a numeric input box. Enter the time. [OK] Tap the button ð The time is saved. ð The dialog box closes. 40/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 41: Editing The Vapor Sensor Sensitivity

    The display shows the dialog box Vapor Sensors . [Vapor alarm sensitivity] Tap the drop-down list next Select the sensitivity value you wish to use. [Close] Tap the button ð The dialog box closes. Operation Manual Pure Chromatography Instrument C-805 41/72...

  • Page 42: Saving A Method

    Navigate to the Solvent Definition dialog according to the navigation path. ð The display shows the dialog box Solvent Definition . [Add Solvent] Tap the button ð The display shows the dialog box Solvent . 42/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 43: Deleting A Solvent

    ð The display shows the dialog box Solvent Definition . Select the solvent you wish to delete. [Delete Solvent] Tap the button [Yes] Answer the secure question with ð The solvent is deleted. Operation Manual Pure Chromatography Instrument C-805 43/72...

  • Page 44: Tasks During A Separation

    6.4.1 Injecting a sample into the flash system Injecting a sample into the cartridge Remove the solvent line from the cartridge. Connect the syringe to the cartridge. Press the plunger. Disconnect the syringe. 44/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 45: Installing And Removing A Cartridge

    The indication for recognizing the cartridge is a sound. Open the solvent line at the point indicated. Hold the cartridge above the RFID reader. Wait until the instrument has taken over the cartridge data. Attach the cartridge to the cartridge holder. Operation Manual Pure Chromatography Instrument C-805 45/72...

  • Page 46: Inserting The Fraction Collection Tray

    The display shows the dialog box Open Method . Select the method you wish to use. The display highlights the selected method black. [OK] Tap the button ð The method is selected. ð The dialog box closes. 46/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 47: Selecting Values On The Dialog Box Sample Loading

    According the requirements adjust the file name. [OK] Tap the button ð The display shows the dialog box Sample Loading . According the requirements adjust the settings. See Chapter 6.4.5 "Selecting values on the dialog box Sample Loading", page 47 Operation Manual Pure Chromatography Instrument C-805 47/72...

  • Page 48: Changings During A Separation

    6.6 Performing a Flash separation manually 6.6.1 Preparing the instrument Time required: approx. 30 sec. Precondition: All commissioning operations have been completed. See Chapter 5 "Installation", page 18 Switch the On/Off master switch to On. 48/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 49: Starting Flash Separation Manually

    Possibilities to edit the gradient during a separation: — Change the gradient. See Editing the gradient in graphic mode — Hold the gradient. See Gradient panel — Auto gradient hold. See Gradient panel Operation Manual Pure Chromatography Instrument C-805 49/72...

  • Page 50: Ending A Flash Separation

    [Shut down] Tap the action [Yes] Confirm the secure question with ð The instrument is shutting down. 6.7 Identifying fractions 6.7.1 Identifying fractions by peak Fig. 7: Identifying fractions 50/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 51: Identifying Fractions Per Vial

    ➔ File Print Run Report Precondition: The instrument is in past run mode. Navigate to the Print Run Report dialog according to the navigation path. ð The display shows the Windows® print dialog. Operation Manual Pure Chromatography Instrument C-805 51/72...

  • Page 52: Sending Data To Usb

    Navigate to the Run panel according the navigation path. [PDF to USB] Tap the button ð The instrument saves a PDF file to the USB storage device. Confirm the complete message. ð The data is saved. 52/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 53: Cleaning And Servicing

    Any servicing and repair work which involves opening up the casing may only be carried out by BUCHI service technicians. Use only genuine BUCHI consumables and spare parts in order to ensure correct operation of the device and preserve the warranty.

  • Page 54: Carrying Out A Data Backup

    7.4 Cleaning the check valve NOTE Most check valve problems can be solved by pumping a strong solution of liquid laboratory grade detergent through the check valves at a rate of 20 mL/min for one hour. 54/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 55: Cleaning The Instrument

    ➔ ➔ Tools Manual Control Column Flushing... Precondition: A purging solvent is assigned to the instrument. See Chapter 5.6 "Assigning solvent to solvent lines", page 20 Install a bypass. See Installing and removing a column Operation Manual Pure Chromatography Instrument C-805 55/72...
  • Page 56 Büchi Labortechnik AG 7 | Cleaning and servicing Navigate to the Column Flushing dialog according to the navigation path. Enter the required data according to your needs. 56/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 57: Help With Faults

    Open or close time of the inlet Rinse the pump module with or outlet valves are not correct high flowrate with ethanol or hot distilled water. Residues in solvent Change check valves. Sealing abrasion outlet valve Operation Manual Pure Chromatography Instrument C-805 57/72...

  • Page 58: Faults, Possible Causes And Solutions (Fraction Collection)

    Fraction collector arm motor is Tighten the motor coupler. slipping Fraction collector arm is ob- Check for cable or burr in the structed fraction collector arm path and remove any obstruction. 58/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 59: Faults, Possible Causes And Solutions (Detection)

    Instrument Alarm: instrument. UV detector: Bad No lamp Contact BUCHI Customer block of High Volt- Hardware error UV detector Service. age (HV) genera- tor for lamp or dis- connected lamp or bad lamp. Operation Manual Pure Chromatography Instrument C-805 59/72...
  • Page 60 Check the lamp and restart Instrument Alarm: Lamp defective/ Flow cell instrument. UV detector: Bad dirty identification of light peak caused by unworkable light intensity. 60/72 Operation Manual Pure Chromatography Instrument C-805...
  • Page 61 Using Default Sol- Solvent file has been Restart the instrument. vent List: System deleted defaults used in- stead of actual re- source. Operation Manual Pure Chromatography Instrument C-805 61/72...
  • Page 62 Solvent Pressure Solvent pressure limit has Remove column. Replace with bypass. been exceeded Determine if column is not the source of blockage. If not column is the source of blockage, then check all fluidic lines. 62/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 63: Resetting The Fraction Collector Arm

    ➔ ➔ Tools Manual Control Fraction Collector Arm Reset Navigate to the Fraction Collector Arm Reset dialog according to the navigation path. Confirm the secure question. ð The Fraction collector arm is resetted. Operation Manual Pure Chromatography Instrument C-805 63/72...

  • Page 64: Taking Out Of Service And Disposal

    When disposing, observe the disposal regulations of the materials used. Materials used see Chapter 3.5 "Technical data", page 14 9.3 Returning the instrument Before returning the instrument, contact the BÜCHI Labortechnik AG Service Department. https://www.buchi.com/contact 64/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 65: Appendix

    Appendix | 10 10 Appendix 10.1 Spare parts and accessories Use only genuine BUCHI consumables and spare parts in order to ensure correct, safe and reliable operation of the system. NOTE Any modifications of spare parts or assemblies are only allowed with the prior written permission of BUCHI.

  • Page 66: Spare Parts

    Pure Glass Tubes 16 x 150 mm 148623416 1000 pcs. Pure Glass Tubes 18 x 150 mm 148623410 500 pcs. Pure Glass Tubes 25 x 150 mm 148623411 500 pcs. Pure Squared bottles 480 mL 148623412 24 pcs. 66/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 67: Wear Parts

    10.3 Finding out separation conditions with the navigator 10.3.1 Open the navigator Navigation path ➔ Gradient panel Navigate to the Gradient panel according the navigation path. [Navigator] Tap the button ð The display shows the dialog Navigator . Operation Manual Pure Chromatography Instrument C-805 67/72...

  • Page 68: Tlc Silica

    The display shows the default flow rate. Adjust the flow rate according to your needs. Select the radio button for Speed or Purity. [Calculate] Tap the button ð The display shows the results. 68/72 Operation Manual Pure Chromatography Instrument C-805...

  • Page 69: Lc-C18

    Enter the retention times for each separation under each chromatograph. Select the column you wish to use from the drop-down list ð The column is selected. ð The display shows the default flow rate. Operation Manual Pure Chromatography Instrument C-805 69/72...

  • Page 70: Lc-Transfer

    Select the cartridge you wish to use for the Flash separation from the drop-down list. ð The cartridge is selected. ð The display shows the default flow rate. 70/72 Operation Manual Pure Chromatography Instrument C-805...
  • Page 71 Tap the button ð The display shows the results. Adjust the following values if necessary: — Flow rate [Accept] Tap the button ð The gradient is saved for a run. ð The dialog box closes. Operation Manual Pure Chromatography Instrument C-805 71/72...
  • Page 72 We are represented by more than 100 distribution partners worldwide. Find your local representative at: www.buchi.com...

Table of Contents