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Summary of Contents for Buchi Pure Chromatography C-900
- Page 1 Pure Chromatography C-900 Operation Manual...
- Page 2 CH-9230 Flawil 1 E-Mail: quality@buchi.com BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experience, especially with respect to structure, illustrations and technical details. This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for competitive purposes, nor made available to third parties.
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Page 3: Table Of Contents
Installing the Pure Fraction Collector.................... 18 Installing the cartridge holder...................... 18 Establishing solvent connections....................... 19 Establishing electrical connections .................... 20 5.6.1 Establishing power supply connections ................ 21 5.6.2 Establishing signal connections ................... 21 Installing the waste line........................ 22 Operation Manual Pure Chromatography C-900... - Page 4 Cleaning and servicing ........................ 44 Regular maintenance work ........................ 44 Cleaning the housing ......................... 44 Cleaning and servicing the warning and directive symbols ............... 44 Cleaning the solvent lines and nozzle .................... 44 Creating a system backup ......................... 45 Operation Manual Pure Chromatography C-900...
- Page 5 Büchi Labortechnik AG Table of contents Help with faults .......................... 46 Sending a log file to BUCHI customer service................... 46 Faults, possible causes and solutions .................... 46 9.2.1 General .......................... 46 9.2.2 Solvent delivery........................ 47 9.2.3 Fraction collection ........................ 48 Customer service.......................... 48 Taking out of service and disposal....................
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Page 6: About This Document
Descriptions referring to these options are only applicable if they are installed. NOTE Deviating interface due to options The interface in this operational manual is shown with all connected options. Depending on the connected options, the interface may deviate slightly. 6/54 Operation Manual Pure Chromatography C-900... -
Page 7: Safety
The operator is responsible for damages or hazards that are caused by use other than that intended. Especially the following uses are not permitted: — Use of the instrument with non-BUCHI instruments. — Use of the instrument in overpressure situations. —... -
Page 8: Warning Symbols
Safety-related incidents that occur while using the instrument should be reported to the manufacturer (quality@buchi.com). BUCHI service technicians Service technicians authorized by BUCHI have attended special training courses and are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair measures. -
Page 9: Aggressive Solvents
Unauthorized modifications can affect safety and lead to accidents. Use only genuine BUCHI accessories, spare parts and consumables. Carry out technical changes only with prior written approval from BUCHI. Only allow changes to be made by BUCHI service technicians. BUCHI accepts no liability for damage, faults and malfunctions resulting from unauthorized modifications. -
Page 10: Product Description
Fig. 1: Pure Chromatography C-900 The instrument works within a modular chromatography system designed to purify complex samples by flash chromatography. Flash chromatography has the ability to separate gram sized samples in a short period of time. 10/54 Operation Manual Pure Chromatography C-900... - Page 11 Pure Fraction Collector (optional) NOTE Pure UV Detector can only operate in conjunction with an appropriate Pure system able to control Pure UV Detector (e.g. Pure Chromatography C-900). The complete upgrade additionally includes Pure Fraction Collector. The chromatography system allows: —...
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Page 12: Front View
Büchi Labortechnik AG 3 | Product description 3.2 Front view Fig. 3: Front view Interface Solvent line outlet USB ports 12/54 Operation Manual Pure Chromatography C-900... -
Page 13: Rear View
The scope of delivery depends on the configuration of the purchase order. Accessories are delivered as per the purchase order, order confirmation, and delivery note. 3.5 Type plate The type plate identifies the instrument. The type plate is located at the rear of the instrument. Operation Manual Pure Chromatography C-900 13/54... -
Page 14: Technical Data
2 BUCHI standard communi- cation ports (COM) 2 USB ports 1 Ethernet port 3.6.2 Ambient conditions For indoor use only. Specification Value Max. altitude above sea level 2,000 m Ambient and storage temperature 5 − 40 °C 14/54 Operation Manual Pure Chromatography C-900... -
Page 15: Materials
The installation site meets the specifications according to the technical data (e.g. weight, dimension, etc.). See Chapter 3.6 "Technical data", page 14. — The installation site and the instrument meet the requirements for the EMC environment, Basic electromagnetic environment / Emission Class B. Operation Manual Pure Chromatography C-900 15/54... -
Page 16: Transport And Storage
Make sure that the ambient conditions are complied with (see Chapter 3.6 "Technical data", page 14). Wherever possible, store the instrument in its original packaging. After storage, check the instrument, all seals and tubing for damage and replace if necessary. 16/54 Operation Manual Pure Chromatography C-900... -
Page 17: Installation
Loosen the screw on the cover. Remove the cover. Mount the cover on the back of the Pure Chromatography C-900 with two screws. Place the Pure UV Detector back into the cover. Tighten the screw on the cover again. Operation Manual Pure Chromatography C-900 17/54... -
Page 18: Installing The Pure Fraction Collector
Only place bottles on top of Pure Fraction Collector at your own risk. 5.4 Installing the cartridge holder An optional cartridge holder can be installed. Carefully place the instrument on a straight surface. Mount the cartridge holder onto the bottom with four screws. 18/54 Operation Manual Pure Chromatography C-900... -
Page 19: Establishing Solvent Connections
Attach the two solvent lines A and B to the Pure Chromatography C-900. Place the other ends of the solvent lines into the solvent bottles. Attach the solvent line from the front of the Pure Chromatography C-900 to the other solvent line. Operation Manual Pure Chromatography C-900 19/54... -
Page 20: Establishing Electrical Connections
Risk of instrument damage because of not suitable power supply cables. Not suitable power supply cables can cause bad performance or an instrument damage Use only BUCHI power supply cables. NOTE Instruments must only be connected/disconnected under certain circumstances. the instrument is powered off... -
Page 21: Establishing Power Supply Connections
Once the instruments are installed and connected, they are ready for use. No further software configuration is required. Connect the signal cable between the Pure Chromatography C-900 and the Pure UV Detector. Connect the signal cable between the Pure UV Detector and the Pure Fraction Collector. Operation Manual Pure Chromatography C-900 21/54... -
Page 22: Installing The Waste Line
Büchi Labortechnik AG 5 | Installation 5.7 Installing the waste line Place the waste line from the solvent line outlet (OUT) on the Pure Fraction Collec- tor into the waste bottle. 22/54 Operation Manual Pure Chromatography C-900... -
Page 23: Software
See Chapter 6.4 "System menu", page 25. Home menu Startup menu with support button. See Chapter 9.1 "Send- ing a log file to BUCHI customer service", page 46. Methods menu To view the method library, create and edit methods. See Chapter 7.4 "Performing a separation using a method", page 32. -
Page 24: Function Buttons
To confirm an input. [Confirm] 6.3 Entering values Numbers and text can be entered directly on the interface. Tap an entry field. ð An input dialog appears. Enter the value. Confirm the value. 24/54 Operation Manual Pure Chromatography C-900... -
Page 25: System Menu
Setting Explanation [System volume] To change the system volume. To switch audible keyboard clicks on/off. [Keyboard clicks] [System] Navigate to the submenu according to the navigation path. [Sound] Select Adjust the settings as desired. Operation Manual Pure Chromatography C-900 25/54... -
Page 26: Changing The Connection Settings
To set a name for the instrument's hotspot. [Password] To set a password for the instrument's hotspot. [Connections] Navigate to the submenu according to the navigation path. [Personal Hotspot] Select Adjust the settings as desired. 26/54 Operation Manual Pure Chromatography C-900... -
Page 27: Customizing Settings
To switch the instrument to manual control. See Chapter 7.10 "Operating in manual control", page 39. Status Setting Explanation [Operational sta- To view the operational status of the instrument. tuses] [Peripherals] To view the connection status to peripherals (Wifi, Ethernet). Operation Manual Pure Chromatography C-900 27/54... -
Page 28: Operation
The solvent lines have to be primed with the solvents that will later be used during a separation. Navigate to the Priming dialog according to the navigation path. [Run] Tap the button. Follow the instructions on the interface to perform the procedure. 28/54 Operation Manual Pure Chromatography C-900... -
Page 29: Installing Racks
A delay volume can be set to compensate the tube length between the Pure UV Detector and the Pure Fraction Collector. The default value is 4.9 ml. Navigation path ➔ ➔ ➔ [Configuration] [Configuration] Navigate to the submenu according to the navigation path. Operation Manual Pure Chromatography C-900 29/54... -
Page 30: Tasks During A Separation
Risk of skin damage from solvent inside cartridge Solvent may still be inside the cartridge or solid loader after a completed run. Wear protective equipment. Carefully open the cartridge as solvent may spill out. 30/54 Operation Manual Pure Chromatography C-900... -
Page 31: Injecting A Sample
Pay attention to leakage when removing the solvent line. Make sure to slowly press the plunger when injecting a sample. Wear protective equipment. Precondition: The syringe with the sample is prepared. Remove the solvent line to the Pure Chromatography C-900 from the cartridge. Operation Manual Pure Chromatography C-900 31/54... -
Page 32: Performing A Separation Using A Method
A method is a set of defined separation parameters applied during a run. In the Methods menu, existing methods can be used, adjusted or duplicated. New methods can be created. Menu Explanation [Search methods] To search a method by name or tag. 32/54 Operation Manual Pure Chromatography C-900... -
Page 33: Performing A Separation Manually
The equilibration is running. [Skip] Tap the button. ð The separation starts. 7.5 Performing a separation manually Precondition: The system is prepared. See Chapter 7.2 "Preparing the system", page 28. The sample is prepared. The cartridge is prepared. Operation Manual Pure Chromatography C-900 33/54... -
Page 34: Adjusting The Solvent Parameters
Add a step. Set the percentage of a solvent. ð The other solvent percentage is adjusted automatically. Set the duration. ð The set percentages of the solvents A and B is displayed in the graph. 34/54 Operation Manual Pure Chromatography C-900... -
Page 35: Adjusting The Cartridge Parameters
➔ ➔ UV detection for four wavelengths is available: — 254 nm — 275 nm — 325 nm — 365 nm Navigate to the UV detection parameters panel according to the navigation path. Switch on/off the desired wavelengths. Operation Manual Pure Chromatography C-900 35/54... -
Page 36: Adjusting The Fraction Collection Parameters
The separation runs. ð A dialog appears once the separation is finished. 7.6 Pausing separations Precondition: A separation is in progress. [Pause] Tap the button. When a separation is paused, it can be restarted later. 36/54 Operation Manual Pure Chromatography C-900... -
Page 37: Identifying Fractions
The corresponding vial number is displayed. ð The corresponding vial is highlighted in green. 7.7.2 Identifying fractions by vial Precondition: A separation is finished. Navigate to the Runs menu according to the navigation path. Tap the desired run. Operation Manual Pure Chromatography C-900 37/54... -
Page 38: Editing Methods
The basic information of a method such as name or tags, as well as the parameters can be adjusted. Precondition: The method is not loaded. Navigate to the Methods menu according to the navigation path. Select the method to be adjusted. 38/54 Operation Manual Pure Chromatography C-900... -
Page 39: Analyzing And Deleting Runs
ð The run is deleted. 7.10 Operating in manual control Navigation path ➔ ➔ ➔ Manual Control NOTE In manual control, no security checks are applied. Carefully operate the instrument, considering the applicable parameters. Operation Manual Pure Chromatography C-900 39/54... -
Page 40: Setting Up The Solvents
[Go to Position: Vial No.] [Vial No.] To enter a position and go there. [Go to Position: Next Position] To go to the next position. To go to the waste vial. [Go to waste vial] 40/54 Operation Manual Pure Chromatography C-900... -
Page 41: Importing And Exporting Data
➔ The following format is possible: — .bdmf Precondition: A USB stick with a method is connected to the instrument. Navigate to the Methods menu according to the navigation path. [Options] Tap the button. Operation Manual Pure Chromatography C-900 41/54... - Page 42 Büchi Labortechnik AG 7 | Operation [Import] Select the method to be imported. ð A dialog confirms the import. 42/54 Operation Manual Pure Chromatography C-900...
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Page 43: Exporting A Method
Navigate to the Methods menu according to the navigation path. [Options] Tap the button. [Export] Select the method to be exported. [Export] Tap the button. Select the export location. ð A dialog confirms the export. Operation Manual Pure Chromatography C-900 43/54... -
Page 44: Cleaning And Servicing
Do not carry out any servicing and cleaning operations that involve opening the housing. Use only genuine BUCHI spare parts in order to ensure correct operation and preserve the warranty. Carry out the service and cleaning operations described in this section to extend the lifetime of the instrument. -
Page 45: Creating A System Backup
A USB stick is connected to a USB port. Navigate to the System Backup dialog according to the navigation path. [Run] Tap the button next to Restore Backup . Follow the instructions on the interface to perform the procedure. Operation Manual Pure Chromatography C-900 45/54... -
Page 46: Help With Faults
9 | Help with faults 9 Help with faults 9.1 Sending a log file to BUCHI customer service In case of a problem, a log file can be saved to a USB stick and sent to BUCHI customer service. Navigation path ➔... -
Page 47: Solvent Delivery
Find the blocked lines and sure is higher than replace it. expected Over-tightened fitting Loosen the fitting or replace Blocked columns or fluidic path Locate the component that caused the blockage, repair, or replace the component. Operation Manual Pure Chromatography C-900 47/54... -
Page 48: Fraction Collection
Authorization requires a comprehensive technical training and knowledge of possible dangers which might arise when working at the device. Such training and knowledge can only be provided by BUCHI. The customer service and support offers the following support: —... -
Page 49: Taking Out Of Service And Disposal
When disposing, observe the disposal regulations of the materials used. Materials used see Chapter 3.6 "Technical data", page 14. 10.3 Returning the instrument Before returning the instrument, contact the BÜCHI Labortechnik AG Service Department. https://www.buchi.com/contact Operation Manual Pure Chromatography C-900 49/54... -
Page 50: Appendix
11 | Appendix 11 Appendix 11.1 Spare parts and accessories Use only genuine BUCHI consumables and spare parts in order to ensure correct, safe and reliable operation of the system. NOTE Any modifications of spare parts or assemblies are only allowed with the prior written permission of BUCHI. -
Page 51: Accessories
Büchi Labortechnik AG Appendix | 11 Order no. Image Communication cable. BUCHI COM, 0.9 m, 6p 11070540 Enables connection between Rotavapor® R-300 / R-220 Pro, Interface I-300 / I-300 Pro, Vacuum Pump V-300 / V-600, Recirculating Chiller F-3xx, VacuBox and LegacyBox. - Page 52 Pivoting clamp for GlasPure ID 70 mm (1 pc.) 044861 Pivoting clamp for GlasPure ID 100 mm (1 pc.) 044862 V-Stand with rod, 950 mm 11069158 Luer lock connection set 11058005 Back pressure regulator (2 bar) 044337 52/54 Operation Manual Pure Chromatography C-900...
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Page 53: Sample Introduction Accessories
(20 pcs.) and frits (40 pcs.) Pure Solid loader M set, incl. adapter set, sleeve, 11070505 tubes (20 pcs.) and frits (40 pcs.) Pure sample loop 5 ml (FEP coil) 045222 Pure sample loop 20 ml (FEP coil) 044852 Operation Manual Pure Chromatography C-900 53/54... - Page 54 We are represented by more than 100 distribution partners worldwide. Find your local representative at: www.buchi.com...
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