Buchi Pure C-805 Operation Manual
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Buchi Pure C-805 Operation Manual

Buchi Pure C-805 Operation Manual

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Pure Chromatography Instrument C-805
Operation Manual

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Summary of Contents for Buchi Pure C-805

  • Page 1 Pure Chromatography Instrument C-805 Operation Manual...
  • Page 2 CH-9230 Flawil 1 E-Mail: quality@buchi.com BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experi- ence, especially with respect to structure, illustrations and technical details. This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for com- petitive purposes, nor made available to third parties.

  • Page 3: Table Of Contents

    BÜCHI Labortechnik AG Table of contents Table of contents About this document...................... 6 Warning notices in this document...................... 6 Symbols ...............................  6 1.2.1 Warning symbols ........................ 6 1.2.2 Mark-ups and symbols...................... 6 Trademarks............................ 7 Connected devices .......................... 7 Safety............................ 8 Proper use ............................ 8 Use other than intended ........................
  • Page 4 Table of contents BÜCHI Labortechnik AG Operation.......................... 23 Control panel .............................  23 6.1.1 Layout of control panel...................... 23 6.1.2 Enter value.......................... 24 6.1.3 Menu bar.......................... 25 6.1.4 Conditions panel ........................ 27 6.1.5 Collection panel ........................ 28 6.1.6 Gradient panel ........................ 29 6.1.7 Run panel..........................
  • Page 5 BÜCHI Labortechnik AG Table of contents Help with faults ........................ 55 Faults, possible causes and solutions (general)................ 55 Faults, possible causes and solutions (cartridge)................ 55 Faults, possible causes and solutions (solvent delivery) .............. 55 Faults, possible causes and solutions (fraction collection) .............. 56 Faults, possible causes and solutions (detection) ................

  • Page 6: About This Document

    If you have any questions after reading this operation manual: Contact BÜCHI Labortechnik AG Customer Service. https://www.buchi.com/contact Warning notices in this document Warning notices warn you of dangers that can occur when handling the device. There are four danger levels, each identifiable by the signal word used.

  • Page 7: Trademarks

    BÜCHI Labortechnik AG About this document | 1 R This character draws attention to a requirement that must be met before the in- structions below are carried out. This character indicates an instruction that must be carried out by the user. ð...

  • Page 8: Safety

    — Safety-related incidents that occur while using the device should be reported to the manufacturer (quality@buchi.com). BUCHI service technicians Service technicians authorized by BUCHI have attended special training courses and are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair measures.

  • Page 9: Residual Risks

    Carry out technical changes only with prior written approval from BUCHI. Only allow changes to be made by BUCHI service technicians. BUCHI accepts no liability for damage, faults and malfunctions resulting from unau- thorized modifications. Operation Manual Pure Chromatography Instrument C-805...

  • Page 10: Product Description

    3 | Product description BÜCHI Labortechnik AG Product description Description of function The instrument is a purification device designed to purify complex samples by flash chromatography. — Flash chromatography has the ability to separate gram size samples in short pe- riod.

  • Page 11: Configuration

    BÜCHI Labortechnik AG Product description | 3 Configuration 3.2.1 Front view Fig. 1: Front view Control panel RFID reader On/Off switch USB Port Cartridge holder Flash pump Fraction collection bay 3.2.2 Rear view NOTE All electrical connections are not limited energy. Operation Manual Pure Chromatography Instrument C-805 11/70...
  • Page 12 3 | Product description BÜCHI Labortechnik AG Fig. 2: Rear view Ventilation slot Gradient valve (See Chapter 3.2.3 "Connections on gradient valve", page 13 ) Signal connection LAN port (for external air supply) Power supply connection Fuse On/Off master switch USB ports Type plate (See Chapter 3.3 "Type plate", page 13 12/70...

  • Page 13: Connections On Gradient Valve

    Waste line Solvent line 2 Solvent line 1 Type plate The type plate identifies the instrument. The type plate is located at the rear of the in- strument. Buchi Labortechnik AG 9230 Flawil / Switzerland Type: 10xxxxxxxx Volt: xxx-xxx VAC Frequ.:...

  • Page 14: Scope Of Delivery

    The scope of delivery depends of the configuration of the purchase order. Accessories are delivered as per the purchase order, order confirmation, and deliv- ery note. Technical data 3.5.1 Pure Chromatography Instrument C-805 Pure C-805 Dimension 365 x 570 x 680 mm (W x D x H) Weight 24 kg...

  • Page 15: Ambient Conditions

    BÜCHI Labortechnik AG Product description | 3 UV Detector Pure C-805 UV wavelength range 200 - 400 nm Light sources Deuterium Detector 3.5.2 Ambient conditions For indoor use only. Max. altitude above sea level 2000 m Ambient temperature 5‒40°C (25°C) No maximum performance above 25°C...

  • Page 16: Transport And Storage

    4 | Transport and storage BÜCHI Labortechnik AG Transport and storage Transport NOTICE Risk of breakage due to incorrect transportation Make sure that all parts of the device are safely packed in such a way as to pre- vent breakage, ideally in the original box. Avoid sharp movements during transit.

  • Page 17: Lifting The Instrument

    BÜCHI Labortechnik AG Transport and storage | 4 Lifting the instrument WARNING Danger due to incorrect transportation The possible consequences are crushing injuries, cuts and breakages. The instrument should be transported by two persons at the same time. Lift the instrument at the points indicated. Lift the instrument –...

  • Page 18: Installation

    5 | Installation BÜCHI Labortechnik AG Installation Installation site The installation site must meet the following requirements: — The installation site has a firm, level surface. — The installation site meets the specifications according to the technical data (e.g. weight, dimension, etc.). See Chapter 3.5 "Technical data", page 14 —...

  • Page 19: Establishing Electrical Connections

    Risk of property damage and diminished performance due to use of un- suitable power cables. The power supply cables supplied with the product by BUCHI precisely match the re- quirements of the device. If other power cables that do not meet those requirements are used, the device may be damaged and/or its performance diminished.

  • Page 20: Assigning Solvent To Solvent Lines

    5 | Installation BÜCHI Labortechnik AG Precondition: R Make sure that the instrument is not con- nected to the power supply. Attach all solvent lines to the instrument. Con- nections see Chapter 3.2 "Configuration", page 11 Put the other end of the solvent line into the solvent bottle.

  • Page 21: Assembling The Waste Bottle

    BÜCHI Labortechnik AG Installation | 5 Assembling the waste bottle NOTICE Waste bottle on top of the instrument Waste bottle on top of the instrument can cause property damages. Make sure that there is a decline between the connection on the instrument and the waste bottle.
  • Page 22 5 | Installation BÜCHI Labortechnik AG Attach the drain line adapter to the solvent bottle platform Attach the drain line to the drain line adapter. Attach the solvent bottle platform to the instru- ment with screws. 22/70 Operation Manual Pure Chromatography Instrument C-805...

  • Page 23: Operation

    BÜCHI Labortechnik AG Operation | 6 Operation Control panel 6.1.1 Layout of control panel Fig. 6: Display Description Function [Menu] bar Shows the available menus. See Menu bar [Conditions] panel Shows the properties and default set- tings of the installed column / cartridge. See Conditions panel [Wavelength] panel Shows available wavelengths and scan...
  • Page 24 6 | Operation BÜCHI Labortechnik AG Description Function [Detector settings] panel Shows selectable detector options and its settings. See Detector selection panel [Slope detection] panel Shows selectable slope detection op- tions. See Chapter 6.1.10 "Slope detection panel", page 32 6.1.2 Enter value Enter numbers Tab on an entry field.
  • Page 25 BÜCHI Labortechnik AG Operation | 6 6.1.3 Menu bar The following menus are available: Menu Submenu / Action Explanation [File] [Open Method] Load an existing method. See Chapter 6.4.4 "Selecting an existing method", page 44 [Save Method] Save an edited method. See Chapter 6.2.8 "Saving a method", page 40 [Save Method as] Save an edited method with another...
  • Page 26 Override max. pressure limit for car- tridges. [UV Baseline] Adjust the baseline to zero during a run. [Language] Select a language. [Service] BUCHI service technicians only [Help] [About] The display shows instrument details. [View Manual] The display shows the Operation man- ual.
  • Page 27 BÜCHI Labortechnik AG Operation | 6 6.1.4 Conditions panel Information button (Information about the selected col- umn) The conditions area contains the following settings: Action Option Explanation [Column] Automatic via RFID Shows the name of the cartridge in- stalled in the instrument. [Flow Rate] Enter value Edit the default flow rate.
  • Page 28 6 | Operation BÜCHI Labortechnik AG 6.1.5 Collection panel Tray number The collection vial matrix corresponds to the trays. The trays are detected by the auto recognition. The estimated number of vials required for the separation is displayed below the solvent usage list in the lower left corner of the Setup window. The fraction collection area contains the following settings: Action Option...
  • Page 29 BÜCHI Labortechnik AG Operation | 6 6.1.6 Gradient panel Edit the gradient and see separation details. NOTE According to the instrument status different options are available. Action Option Explanation [Table] Create a gradient See Editing the gradient in tabular mode [Navigator] Finding out Flash sepa- See Chapter 10.3 "Finding out sepa-...
  • Page 30 6 | Operation BÜCHI Labortechnik AG Action Option Explanation [Auto gradient hold] Function The gradient will be held every time the signal goes over the set thresh- old. 6.1.7 Run panel The run panel shows available functions according to the current operation. Symbol Description Meaning...

  • Page 31: Detector Selection Panel

    BÜCHI Labortechnik AG Operation | 6 6.1.8 Solvent selection panel Select solvents for a separation. See Chapter 6.2.2 "Selecting a solvent", page 33 6.1.9 Detector selection panel Action Option Explanation [UV] Enable / Disable Action enabled: Select value / Enter Low / High value Enter values for threshold Action disabled:...

  • Page 32: Slope Detection Panel

    Selecting a cartridge (Flash mode) NOTE The automatic reading of the cartridge information can only be done with specific BUCHI RFID tagged cartridges. 4 The indication for recognizing the cartridge is a sound. 32/70 Operation Manual Pure Chromatography Instrument C-805...

  • Page 33: Selecting A Solvent

    BÜCHI Labortechnik AG Operation | 6 Information button (Information about the selected col- umn) Navigation path ➔ Conditions panel Navigate to the Conditions selection panel according the navigation path. Tap the input box next to [Column]. ð The display shows a menu with selectable cartridges. Select the cartridges you wish to use.

  • Page 34: Editing The Gradient

    6 | Operation BÜCHI Labortechnik AG Navigation path ➔ Solvent selection panel Precondition: R The required solvents lines are connected and assigned. See Chapter 5.6 "As- signing solvent to solvent lines", page 20 Navigate to the Solvent selection panel according the navigation path. Tap the drop-down list next to A: .
  • Page 35 BÜCHI Labortechnik AG Operation | 6 Action Option Explanation [Min] Enter value Enter the time until the value in col- umn [% 2nd] is reached. [Solvents] Select value Select solvent line combinations. [% 2nd] Enter value Enter the percentage rate for the second solvent.

  • Page 36: Editing The Wavelength For The Uv Detector

    6 | Operation BÜCHI Labortechnik AG Add steps to the Gradient graphic Tap on the line at the time for which you want to edit the gradient and drag it to the desired %B, then release. Deleting steps from the Gradient graphic To remove a step, drag the point to the baseline or to any gray area around the graph until a red X is visible, then release.

  • Page 37: Editing The Fraction Collection Criteria

    BÜCHI Labortechnik AG Operation | 6 6.2.5 Editing the fraction collection criteria Identifier The following fraction collection criteria are available: Criteria Meaning [Collect Peaks] The instrument collects fractions if one detector signal is above the set threshold. [Collect All] The instrument collects all the fractions regardless of de- tection signals.

  • Page 38: Editing The Fraction Collection Time

    6 | Operation BÜCHI Labortechnik AG Tap the button [OK]. ð The dialog box closes. 6.2.6 Editing the fraction collection time Program Collect allows to turn off fraction collection for a specific time. The fraction collector defaults to collect the full run length unless the values from Pro- gram Collect override the collection.

  • Page 39: Editing The Vapor Sensor Sensitivity

    BÜCHI Labortechnik AG Operation | 6 Add additional lines to the program collection Tap the number field (1►). ð The display shows a drop-down list with selectable actions. Select if the line should be added above or below the selected line. ð...

  • Page 40: Saving A Method

    6 | Operation BÜCHI Labortechnik AG 6.2.8 Saving a method Navigation path ➔ File ➔ Save Method as Navigate to the Save Method as dialog according to the navigation path. ð The display shows the dialog box Save Method as. Tap the input box [Enter method name].

  • Page 41: Deleting A Solvent

    BÜCHI Labortechnik AG Operation | 6 Tap the input box next to [Name]. ð The display shows a dialog with an alphanumeric input box. Enter the name for the solvent. Tap the button [OK]. ð The dialog box closes. Tap the input box next to [Info]. ð...

  • Page 42: Tasks During A Separation

    6 | Operation BÜCHI Labortechnik AG Tasks during a separation 6.4.1 Injecting a sample into the flash system Injecting a sample into the cartridge Remove the solvent line from the cartridge. Connect the syringe to the cartridge. Press the plunger. Disconnect the syringe.

  • Page 43: Installing And Removing A Cartridge

    Instead of a cartridge a bypass can be installed. NOTE The automatic reading of the cartridge information can only be done with specific BUCHI RFID tagged cartridges. 4 The indication for recognizing the cartridge is a sound. Open the solvent line at the point indicated.

  • Page 44: Inserting The Fraction Collection Tray

    6 | Operation BÜCHI Labortechnik AG Attach the solvent lines to the cartridge. 6.4.3 Inserting the fraction collection tray NOTE By default the first available tube on each tray is reserved for waste. Changing the setting. See Chapter 6.4.5 "Selecting values on the dialog box Sample Loading", page 45 Optical sensor slot Place the collection tubes in the collection tray.

  • Page 45: Selecting Values On The Dialog Box Sample Loading

    BÜCHI Labortechnik AG Operation | 6 6.4.5 Selecting values on the dialog box Sample Loading The display shows the dialog box Sample Loading during the starting phase of a separation. The following settings are possible: Action Explanation [Lower flow rate for Reduce flow rate for a specified period, when the pressure specified time after in- increases significantly at the start of the run after sample...

  • Page 46: Changings During A Separation

    6 | Operation BÜCHI Labortechnik AG Follow the instructions on the display. ð Installing the cartridge. See Chapter 6.4.2 "Installing and removing a car- tridge", page 43 ð Introducing the sample into the system. See Chapter 6.4.1 "Injecting a sample into the flash system", page 42 6.5.3 Changings during a separation NOTE...

  • Page 47: Starting Flash Separation Manually

    BÜCHI Labortechnik AG Operation | 6 Switch the On/Off master switch to On. Tap the On/Off switch. ð The instrument is starting up. 6.6.2 Starting Flash separation manually Precondition: R The instrument is prepared. See Chapter 6.6.1 "Preparing the instrument", page 46 R The required solvents are connected and assigned.

  • Page 48: Ending A Flash Separation

    6 | Operation BÜCHI Labortechnik AG 6.6.4 Ending a Flash separation NOTE The separation extends automatically by 5 minutes if the baseline at the end of the separation is not below the set threshold. Precondition: R The display shows the dialog box Separation End. According to the requirements extend the separation time by 5 minutes.

  • Page 49: Identifying Fractions Per Vial

    BÜCHI Labortechnik AG Operation | 6 Navigation path ➔ Gradient panel Precondition: R A separation is finished. Navigate to the Gradient panel according the navigation path. Tap the peak with the target value. ð The Collection panel shows the corresponding vial. 6.7.2 Identifying fractions per vial Fig. 8: Identifying fractions...

  • Page 50: Sending Data To Usb

    6 | Operation BÜCHI Labortechnik AG Select your printer. Tap the button [OK]. ð The report is printed. 6.8.2 Sending data to USB Navigation path ➔ Run panel Precondition: R The instrument is in past run mode. Connect a USB storage device to the instrument. Navigate to the Run panel according the navigation path.

  • Page 51: Cleaning And Servicing

    Any servicing and repair work which involves opening up the casing may only be car- ried out by BUCHI service technicians. Use only genuine BUCHI consumables and spare parts in order to ensure correct operation of the device and preserve the warranty.

  • Page 52: Carrying Out A Data Backup

    7 | Cleaning and servicing BÜCHI Labortechnik AG Carrying out a data backup Navigation path ➔ File ➔ Exit Navigate to the Exit dialog according to the navigation path. Confirm the secure question. ð The Pure software is shutting down. ð...

  • Page 53: Cleaning The Instrument

    BÜCHI Labortechnik AG Cleaning and servicing | 7 Pumping detergent through the check valves Materials needed: — Liquid Laboratory Detergent — Isopropanol/Water (50/50) or Methanol/Water (50/50) Navigation path ➔ Tools ➔ Manual Control ➔ Column Flushing... Precondition: R A bypass is installed. See Installing and removing a column Navigate to the Column Flushing dialog according to the navigation path.
  • Page 54 7 | Cleaning and servicing BÜCHI Labortechnik AG Install a bypass. See Installing and removing a column Navigate to the Column Flushing dialog according to the navigation path. Enter the required data according to your needs. 54/70 Operation Manual Pure Chromatography Instrument C-805...

  • Page 55: Help With Faults

    BÜCHI Labortechnik AG Help with faults | 8 Help with faults Faults, possible causes and solutions (general) Malfunction Possible cause Solution The instrument Power is not being supplied to Verify that the power cord does not power up the system is plugged in.

  • Page 56: Faults, Possible Causes And Solutions (Fraction Collection)

    8 | Help with faults BÜCHI Labortechnik AG Malfunction Possible cause Solution Inconsistent solvent Loose fitting/air leak into the Find the loose fitting be- flow pump tween mobile phase reser- voir manifold and pump in- let fitting and tighten it up. Liquid leak/pump seals worn Fix the leak/replace the pump seals.

  • Page 57: Faults, Possible Causes And Solutions (Detection)

    Serial communication dis- Restart the instrument. Solvent pump: turbed Communication to the pump cannot be established. Instrument Alarm: Contact BUCHI Customer Solvent pump: Mo- Service. tor overloading. Instrument Alarm: Hardware error solvent pump Contact BUCHI Customer Solvent pump: Mo- Service.
  • Page 58 Instrument Alarm: Lamp defect Check the lamp and restart UV detector: Bad instrument. No lamp block of High Volt- Contact BUCHI Customer Hardware error UV detector age (HV) generator Service. for lamp or discon- nected lamp or bad lamp. Instrument Alarm:...
  • Page 59 BÜCHI Labortechnik AG Help with faults | 8 Error message Possible cause Solution Instrument Alarm: Hardware error UV detector Contact BUCHI Customer UV detector: Low Service. light intensity was found on some photo elements of CCD sensor. Instrument Alarm: Hardware error UV detector...
  • Page 60 Purge lines/system with a strong or appropriate sol- vent that will dissolve the sample Valve stuck in incorrect posi- Reset valve position. tion Contact BUCHI Customer Service. Error in solvent line Calibration file of solvent lines Shutdown the Pure soft- calibration file. damaged ware.

  • Page 61: Resetting The Fraction Collector Arm

    See Chapter 8.7 "Resetting the Fraction col- lector arm", page 61 Fraction collector arm did not Tighten the motor coupler. home properly Contact BUCHI Customer Service. Fraction collector arm motor is Contact BUCHI Customer slipping Service. Home position sensor is bad Contact BUCHI Customer Service.

  • Page 62: Taking Out Of Service And Disposal

    When disposing, observe the disposal regulations of the materials used. Materials used see Chapter 3.5 "Technical data", page 14 Returning the instrument Before returning the instrument, contact the BÜCHI Labortechnik AG Service Depart- ment. https://www.buchi.com/contact 62/70 Operation Manual Pure Chromatography Instrument C-805...

  • Page 63: Appendix

    Appendix 10.1 Spare parts and accessories Use only genuine BUCHI consumables and spare parts in order to ensure correct, safe and reliable operation of the system. NOTE Any modifications of spare parts or assemblies are only allowed with the prior written permission of BUCHI.

  • Page 64: Spare Parts

    10 | Appendix BÜCHI Labortechnik AG Order no. Image Solvent bottle platform kit 11069285 Solvent bottle platform on top of the instrument. For maximum 4 bottles (volume 4 L each) Retaining container 11068468 Retaining container for solvent bottle platform for more safety regarding leaking Loading Pump 11071418...

  • Page 65: Wear Parts

    4 Navigate to folder options via the following navigation path: View ➔ Folder Op- tions ➔ View 4 Activate the function [Show hidden files, folders and drives]. Explanation Type Folder Method files .gfm C:\Users\Public\Documents\Buchi\Pure \methods Run files .gkfr C:\Users\Public\Documents\Buchi\Pure\runs 10.3 Finding out separation conditions with the navigator 10.3.1...

  • Page 66: Tlc Silica

    10 | Appendix BÜCHI Labortechnik AG 10.3.2 TLC Silica The TLC-Silica tool uses TLC separation data to provide a recommended gradient for silica separations of 2 or 3 components. Specification of the samples needed: — Two different solvent concentrations — Two separations —...

  • Page 67: Lc-C18

    BÜCHI Labortechnik AG Appendix | 10 Adjust the following values if necessary: — Column — Flow rate — Speed / Purity Tap the button [Accept]. ð The gradient is saved for a run. ð The dialog box closes. 10.3.3 LC-C18 The LC-C18 Tool uses isocratic HPLC separation data to provide a recommended gradient for reversed phase separations of 2 components.

  • Page 68: Lc-Transfer

    10 | Appendix BÜCHI Labortechnik AG Adjust the flow rate according to your needs. Select the radio button for Speed or Purity. Tap the button [Calculate]. ð The display shows the results. Tap the button [Accept]. ð The gradient is saved for a run. ð...
  • Page 69 BÜCHI Labortechnik AG Appendix | 10 Adjust the flow rate according to your needs. Tap the button [Calculate]. ð The display shows the results. Adjust the following values if necessary: — Flow rate Tap the button [Accept]. ð The gradient is saved for a run. ð...
  • Page 70 T +971 4 313 2860 T +52 55 9001 5386 F +66 2 862 08 54 F +971 4 313 2861 latinoamerica@buchi.com bacc@buchi.com middleeast@buchi.com www.buchi.com/es-es www.buchi.com/th-th www.buchi.com We are represented by more than 100 distribution partners worldwide. Find your local representative at: www.buchi.com...

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