Motic BA310E Instruction Manual
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Motic BA310E Instruction Manual

Biological microscope
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Summary of Contents for Motic BA310E

  • Page 2 We are constantly endeavoring to improve our instruments and to adapt them to the requirements of modern research techniques and testing methods. This involves modification to the mechanical structure and optical design of our instruments. Therefore, all descriptions and illustrations in this instruction manual, including all specifications are subject to change without notice.
  • Page 3 INFINITY CORRECTED OPTICS In this optical concept the light beams are parallel after leaving the objective in the direction of the eyepieces. A second optical element, the tube lens (normally located in the eyepiece tube) is used to converge the parallel beams, resulting in an intermediate image. The intermediate image is focussed by the eyepieces, to provide the real image for visual observation.
  • Page 4 MICROSCOPE TERMINOLOGY Abbe Condenser Magnification A two-lens sub-stage condenser located below The number of times by which the size of the the stage of a microscope and functions to collect image exceeds the original object. Lateral light and direct it onto the object being examined. magnification is usually meant.
  • Page 5 Diopter adjustment Resolving Power The adjustment of the eyepiece of an instrument A measure of an optical system's ability to to provide accommodation for the eyesight produce an image which separates two points or differences of individual observers. parallel lines on the object. Depth of Focus Resolution The axial depth of the space on both sides of the...

  • Page 6: Table Of Contents

    TABLE OF CONTENTS SECTION PAGE NOMENCLATURE SETTING UP THE INSTRUMENT Operating environment ASSEMBLING THE MICROSCOPE Verifying input voltage Illumination 3.2.1 Halogen lamp 3.2.2 Reckless stage Specimen holder Objectives Condenser Eyepiece tube Eyepieces Filters 3.10 Power cord USAGE OF MICROSCOPE COMPONENTS Coarse and fine focusing Coarse focus torque adjustment Coarse focus quick stop...
  • Page 7 5. PHOTOMICROGRAPHIC PROCEDURE 6. USING OIL IMMERSION OBJECTIVES 7. TROUBLESHOOTING TABLE Optical Electrical CARE AND MAINTENANCE Do not disassemble Cleaning the microscope 8.2.1 Lenses and filters 8.2.2 Cleaning of painted or plastic components Disinfecting the Microscope When not in use Bulb replacement 8.5.1 30W Halogen...

  • Page 8: Nomenclature

    1. NOMENCLATURE BA310E (Binocular)
  • Page 9 BA310E (Trinocular)

  • Page 10: Setting Up The Instrument

    2. SETTING UP THE INSTRUMENT Avoid placing the instrument in locations exposed to direct sunlight, dust, vibration, high temperature, high humidity and where it is difficult to unplug the power supply cord. 2.1 Operating environment  Indoor use  Altitude : Max 2000 metersn ...

  • Page 11: Assembling The Microscope

    3. ASSEMBLING THE MICROSCOPE 3.1 Verifying input voltage  The automatic voltage selection works with a broad range of settings. However, always use a power cord that is rated for the voltage used in your area and that has been approved to meet local safety standards.

  • Page 12: Reckless Stage

    3.3 Reckless stage  Remove specimen holder for fast hand scanning of slides.  Left-handed and right-handed operation stages are available for option. Left hand operation stages is available for option. It should be used with the short stage knob for avoiding to interfere the fine focus knob..

  • Page 13: Eyepiece Tube

    3.7 Eyepiece tube  Loosen the eyepiece clamp screw (Fig.1). Insert the round dovetail mount on the eyepiece tube into the round dovetail mount on the microscope arm. Tighten the eyepiece tube clamp screw to secure the eyepiece tube in place. (Fig.1) 3.8 Eyepieces ...

  • Page 14: Filters

    3.9 Filters  Remove the collector cover (Fig.4) and place the filter in the filter holder located around the field lens (Fig.5.1), then screw the cover back (Fig.6). Take care that dust, dirt and fingerprints do not get on the filter and the field lens. (Fig.4) (Fig.5) (Fig.6)

  • Page 15: Power Cord

     Filter selection: Filter Function ND2 (T=50%) ND4 (T=25%) For brightness adjustment in photomicrography ND16 (T=6.25%) Blue filter (colour balance filter) For routine microscopy and photomicrography For phase contrast and contrast adjustment with black and Green interference (546nm) white film For colour photomicrography of HE stained specimen with HE (didymium filter) tungsten type film...

  • Page 16: Usage Of Microscope Components

    4. USAGE OF MICROSCOPE COMPONENTS 4.1 Coarse and fine focusing (Fig.7)  Focusing is carried out with the coarse and fine focus knobs at the left and right of the microscope stand.  The direction of vertical movement of the stage corresponds to the turning direction of the focus knobs.

  • Page 17: Coarse Focus Quick Stop

    4.3 Coarse focus quick stop (Fig.9)  The coarse focus quick stop makes the stage can fixed at any position at which the specimen is in focus i.e. by using the handle to lock the coarse focus knob.  With the specimen in focus, turn the handle to fix the knob. ...

  • Page 18: Beam Splitter Lever

    This adjustment will enable the user to observe the specimen with both eyes  Depending on the working environment and its demands, the BA310E has a solution. Each eyepiece tube allows a 360°swiveling movement (Fig.11) as well as a flexible adjustment of the interpupillary distance between 48 and 75mm.

  • Page 19: Diopter Adjustment

    4.7 Diopter adjustment  Every human eye is different, to adjust the Instrument to best performance adjustment can be necessary.  Set the diopter on both eyepieces to the “0” position.  Change to 10x Magnification and focus the image of the specimen with one eye only. ...

  • Page 20: Use Of Aperture Diaphragm

    4.9 Use of aperture diaphragm  The condenser aperture diaphragm is provided for adjusting the numerical aperture (N.A.) of the illuminating system of the microscope, it decides the resolution of the image, contrast, depth of focus and brightness.  Stopping down will lower the resolution and brightness but increase the contrast and depth of focus. ...

  • Page 21: Photomicrographic Procedure

    5. PHOTOMICROGRAPHIC PROCEDURE  To ensure vibration free operation, set the microscope on a sturdy vibration free table or a bench with a vibration proof device.  Pull the optical path selection lever of the trinocular eyepiece tube all of the way out to the limit, the ratio of light entering the observation tube and phototube will be 0:100.

  • Page 22: Using Oil Immersion Objectives

    Oil immersion objectives are labelled with the additional engraving “Oil” and are to be immersed in oil between the specimen and the front of the objective.  The immersion oil supplied by Motic is synthetic, non-fluorescing and non-resining oil, with a refractive index of 1.515. ...

  • Page 23: Troubleshooting Table

    7. TROUBLESHOOTING TABLE As you use your microscope, you may occasionally experience a problem. The troubleshooting table below contains the majority of frequently encountered problems and the possible causes. 7.1 Optical Problem Possible Cause Lamp not installed properly Lamp not centred Diffuser is in intermediate position Condenser not mounted correctly Condenser is not centred...

  • Page 24: Electrical

    Stage installed on inclined plane Unequal focus Specimen holder not fixed securely on stage Specimen not secured in position Lamp voltage is set too low Image tinged yellow Blue filter is not being us Slide is upside down Focusing is not possible with high magnification objectives Cover glass is too thick Slide is upside down...

  • Page 25: Care And Maintenance

    Store the objectives, eyepieces and filters in a container or desiccator with drying agent.  Proper handling of the microscope will ensure years of trouble free service.  If repair becomes necessary, please contact your Motic agency or our Technical Service direct.

  • Page 26: Bulb Replacement

    Note:  If equipment is used in a manner not specified by the manufacturer, the warranty may be void.  To avoid getting wet, do not use the microscope near water. 8.5 Bulb replacement The lamp and the lamphouse become very hot during and after a period of operation. Risk of burn –...

  • Page 27: Led Module

    Place microscope on its back and pull back the lamp house cover plate. (Fig.17)  Firmly insert the LED module into the socket pinholes until it reaches the limit (Fig.18). This is a Motic patent design to exchange LED module and halogen bulb on the same socket directly. ...
  • Page 28 (Fig.19) (Fig.20)

  • Page 29: Warning Labels

    Make sure the lamp has cooled sufficiently before attempting to replace the lamp. Don’t pick the microscope up from the bottom during equipment operation. Proper handling of the microscope will ensure years of trouble free service. If repair become necessary, please contact your Motic agency or our Technical Service directly.

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