Pre-Freezing Samples; Adding Sample - Labconco FreeZone 70061 6L -50C Series User Manual

Chapter 5: Using Your Freeze Dryer
on heated shelves or in glassware attached to manifold valves, the volatility of the
sample itself, the size of the sample and the heat energy supplied to the sample.
Some guidelines for setting the vacuum level are shown below. Exact protocols
must be determined by the user for the specific samples that are being freeze dried.
Material
Bacteria,Virus
Milk
Fungi
Vegetable Tissue
Human Tissue
Blood Plasma
Vaccine
When the vacuum control is set to operate at less vacuum (higher pressure), the ice
holding capacity of the collector may be decreased. This can be improved by
installing a baffle in the collector. See Appendix A Freeze Dry Components for the
baffle part number. See Chapter 3: Baffle Installation for proper positioning of the
baffle in the collector chamber.

Pre-Freezing Samples

Appropriate containers for freeze drying include ampules, serum bottles, and wide
mouth freeze drying flasks. Shell freezing of samples is recommended for wide
mouth freeze drying flasks. Smaller samples in ampules and serum bottles may be
frozen in a freezer or in the center of the collector chamber. The sample container
size should always be at least two to three times the sample size (i.e., 40 ml
samples should be prepared in 80 ml containers or larger). The temperature
required for pre-freezing is dependent on the characteristics of the sample. Pre-
freezing temperature is typically at least 10° to 20°C below the eutectic or collapse
temperature of the sample.

Adding Sample

The following procedure should be followed when using chambers or manifolds
with sample valves in the freeze dry process:
1. Connect a pre-frozen sample to a sample valve on the drying chamber or manifold
using an adapter. Turn the plastic valve knob to the "VACUUM" position to open
the valve, which connects the attached sample to system vacuum. The bevel on the
knob should be positioned toward the sample port to apply vacuum to the sample.
38
Solidification/Eutectic
Temperature Temperatures
≤ -40°C
-5 to -13
≤ -40
-25 to -50
-30 to –40
-10 to –25
-30 to –40
Product Service 816-333-8811 or 1-800-522-7658
Pre-Freeze
Vacuum Set Point
≤ -50°C ≤ 0.040 mbar
-15 to -23 1.65 to 0.77
≤ -50 ≤ 0.040
-35 to -60 0.22 to 0.01
-40 to –50
0.12 to 0.04
-20 to –35
1.03 to 0.22
-40 to –50
0.12 to 0.04
Original instructions