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CH-9230 Flawil 1 E-Mail: quality@buchi.com BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experi- ence, especially with respect to structure, illustrations and technical details. This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for com- petitive purposes, nor made available to third parties.
Scope of delivery .......................... 22 Technical data ........................... 22 3.5.1 FatExtractor E-500......................... 22 3.5.2 Ambient conditions......................... 23 3.5.3 Materials .......................... 24 Transport and storage ...................... 25 Transport ............................ 25 Storage .............................. 25 Lifting the instrument ......................... 25 Operation Manual FatExtractor E-500...
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Operating the interface pro .................... 48 Layout of the interface ........................ 48 Function bar............................ 49 Menu bar............................ 49 7.3.1 Home menu ........................... 50 7.3.2 Method menu ......................... 50 7.3.3 Configuration menu........................ 51 7.3.4 Solvent menu ......................... 53 Status bar ............................ 53 Operation Manual FatExtractor E-500...
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Exporting extraction parameters without cloud connection............ 70 9.6.2 Exporting extraction parameters with cloud connection............ 71 Editing a solvent........................ 72 10.1 Creating a new solvent ........................ 72 10.2 Changing the name of a solvent (own created solvents only) ............ 72 Operation Manual FatExtractor E-500...
Indicates a danger that could result in damage to property. Symbols The following symbols are displayed in this operation manual or on the device: 1.2.1 Warning symbols Symbol Meaning General warning Hot surface Breakable items Hand injuries Flammable substances Operation Manual FatExtractor E-500 7/88...
Signals are marked-up like this. Trademarks Product names and registered or unregistered trademarks that are used in this docu- ment are used only for identification and remain the property of the owner in each case. 8/88 Operation Manual FatExtractor E-500...
Safety | 2 Safety Proper use The FatExtractor E-500 is designed for the extraction (solid-liquid extraction) of sam- ples with known solvents and the reduction or drying of the extract. The FatExtractor E-500 can be used in laboratories for the following tasks: ...
Safety-related incidents that occur while using the device should be reported to the manufacturer (quality@buchi.com). BUCHI service technicians Service technicians authorized by BUCHI have attended special training courses and are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair measures.
Make sure that the locking mechanism of the protection shield is working properly. If faults occur: Switch off the device immediately. Unplug the power cord. Inform the operator. Cool the device before taking any further action. Operation Manual FatExtractor E-500 11/88...
Make sure that the decontamination agent is compatible with the materials used. See Chapter 3.5 "Technical data", page 22 If you have any further questions, contact BUCHI Customer Service. 2.6.8 Solvent leakages during extraction process Solvent leaking systems can cause dangerous solvent vapors that lead to instrument damage or fire.
Technical modifications to the device or accessories should only be carried out with the prior written approval of BÜCHI Labortechnik AG and only by authorized BUCHI technicians. BUCHI accepts no liability whatsoever for damage arising as a result of unauthorized modifications. Operation Manual FatExtractor E-500...
3 | Product description BÜCHI Labortechnik AG Product description Description of function The FatExtractor E-500 is designed to carry out the following solid-liquid extraction methods: Soxhlet Extraction (see Chapter 3.1.1 "Soxhlet Extraction", page 14) Hot Extraction (see Chapter 3.1.2 "Hot Extraction", page 15) ...
3.1.4 Description of the Analyte Protection Sensor (option) The Analyte Protection Sensor protects the analyte from overheating. The Analyte Protection Sensor triggers under the following situations: Soxhlet Economic Extraction Extraction Continuous Extraction Presence of beaker Operation Manual FatExtractor E-500 15/88...
3.2.3 Upper connections on the rear side Fig. 4: Connections on the top Standard BUCHI communication port Standard BUCHI communication port (COM) (COM) LAN port Port for the cooling water valve Operation Manual FatExtractor E-500 17/88...
Inert gas connection (option) (marked INERT) Coolant water out Exhaust (marked OUT) (marked EXHAUST) Coolant water in 10 Type plate (marked IN) 11 Coolant water solvent tank out 12 Solvent out (marked OUT) (marked TANK OUT (SOLVENT)) 18/88 Operation Manual FatExtractor E-500...
Product description | 3 3.2.7 Pro Interface Fig. 8: Interface Pro Stop button Touch-screen display Navigation control Type plate The type plate identifies the instrument. The type plate is located at the rear of the in- strument. Operation Manual FatExtractor E-500 21/88...
638 x 595 x 613 mm ( with glassware Hot Extraction) Dimensions (W x D x H) 638 x 595 x 622 mm (with glassware Economic Continuous Extraction) Minimum clearance (W x D) 200 mm Minimum clearance (H) 500 mm 22/88 Operation Manual FatExtractor E-500...
The instrument should be transported by two persons at the same time. Lift the instrument at the points indicated. NOTICE Dragging the instrument can damage the feet of the instrument. Lift the instrument when positioning or re-locating. Operation Manual FatExtractor E-500 25/88...
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4 | Transport and storage BÜCHI Labortechnik AG Fig. 10: Lifting the instrument Lift the instrument – this requires two persons each lifting at the points indicated on the left and right side of the instrument. 26/88 Operation Manual FatExtractor E-500...
1. Provide the mains voltage and frequency specified on the type plate of the instru- ment. 2. Be designed for the load imposed by the instruments connected. 3. Be equipped with suitable fuses and electrical safety features. 4. Be equipped with a proper grounding. Operation Manual FatExtractor E-500 27/88...
Risk of property damage and diminished performance due to use of un- suitable power cables. The power supply cables supplied with the product by BUCHI precisely match the re- quirements of the device. If other power cables that do not meet those requirements are used, the device may be damaged and/or its performance diminished.
IN (SOLVENT) . Attach the inlet tube onto the connection marked OUT . Attach the drain tube onto the connection marked IN (SOLVENT) . Make sure that the solvent collector circuit is complete. See Chapter 14.1 "Schematics", page 81 Operation Manual FatExtractor E-500 29/88...
Risk of broken parts by using tools Using tools for preparing the instrument can break parts. Tighten all parts with your hands. Switch the On/Off master switch to Off. Put the bottle cap on the bottle. 30/88 Operation Manual FatExtractor E-500...
Attach the valve to the extraction glass cham- ber at the point indicated. NOTICE! Make sure that the sensor is not bent during carrying out this action step Attach the sensor to the extraction glass chamber. Operation Manual FatExtractor E-500 31/88...
Soxhlet", page 31 Pull the chamber rack. Precondition: R Start with the extraction glass chamber on the left side. R Make sure that the BUCHI Logo points to the front. Put the extraction glass chamber in the cham- ber rack. 32/88...
Prepare the condenser. See Chapter 5.13 "Preparing the condenser", page 36 Install the protection shields. See Chapter 5.12.2 "Installing one protec- tion shield (Hot and Economic Continuous Extraction only)", page 36 Select the Hot Extraction method on the interface. See Chapter 5.14 "Selecting a configuration", page 39 Operation Manual FatExtractor E-500 33/88...
Installing the extraction glass chamber Economic Continuous Extraction Prepare the extraction glass chamber. See Chapter 5.11.2 "Preparing the extraction glass chamber for an Economic Continuous Extrac- tion", page 34 Pull the chamber rack. Put the extraction glass chamber in the instru- ment. 34/88 Operation Manual FatExtractor E-500...
Make sure, that both protections shields fit to- gether. Hold both protection shields on both sides at the point indicated. Put the rear protection shield on both sides into the rear slot at the same time. Operation Manual FatExtractor E-500 35/88...
Put the protection shield on both sides into the front slot at the same time. 5.13 Preparing the condenser NOTICE Risk of broken parts by using tools Using tools for preparing the instrument can break parts. Tighten all parts with your hands. 36/88 Operation Manual FatExtractor E-500...
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Attach the solvent connection nut with the seal to the condenser. Connect the solvent collector tube. NOTICE! Do not use force to carry out this ac- tion step. Put the condenser in the condenser rack. Operation Manual FatExtractor E-500 37/88...
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Secure the condenser with the flange lock. Turn the condenser. Connect the solvent collector tube to the in- stallation board Attach the tubes with the attachment clip to the installation board. Connect the condensers. Establish a condenser circuit. 38/88 Operation Manual FatExtractor E-500...
ð The dialog box closes. 5.15 Disassembling the condenser circuit Disconnect the indicated condenser connec- tion. Make sure, that there is no coolant in the con- denser circuit. Disconnect the other condenser connections. Remove the flange lock. Operation Manual FatExtractor E-500 39/88...
The following port has to be enabled in the firewall settings on the internet gate- way: TCP (HTTPS) traffics through remote port 443 In order to use the BUCHI Cloud a DNS server must be configured on the instru- ment. NOTE If there is no DNS server available enter the IP address for the BUCHI Cloud connec- tion manually.
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BÜCHI Labortechnik AG Installation | 5 Navigation path ➔ ➔ [Settings] ➔ [Network] Navigate to the action [Network]. Enable the function [DHCP]. ð The instrument is prepared. Operation Manual FatExtractor E-500 41/88...
Pressing the control performs the assigned function on the function bar. Function bar The function bar shows available functions according to the current operation. The functions on the function bar are executed by tapping the relevant function but- tons. 42/88 Operation Manual FatExtractor E-500...
Process control param- [Home] menu eters Editing and saving ex- [Method] menu traction methods Change settings [Configuration] menu Service menu System information Solvent library [Solvent] menu Editing and saving sol- vents Operation Manual FatExtractor E-500 43/88...
[Analyte protection View Threshold for empty-no beaker (option)] Threshold for full-empty beaker [Mobile connection View The control unit shows a QR code QR code] for connecting the Extraction Re- ports app with the instrument. 44/88 Operation Manual FatExtractor E-500...
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Action Option Explanation [Sensors] View All sensor signals can be observed: Flow senors Level sensors Analyte protection (option) Protection shield Valves Lift current Line voltage Line frequency Operation Manual FatExtractor E-500 45/88...
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Board temperature Code 30 V Operating voltage 5 V Operating voltage Submenu History QR code Shows the history list of the data export QR codes to export data. SeeChapter 9.6 "Exporting extraction parameters", page 70 46/88 Operation Manual FatExtractor E-500...
The solvent menu contains the available solvents for the extraction methods. The solvent menu allows editing individual solvents. See Chapter 10 "Editing a sol- vent", page 72 Status bar The status bar shows actual information about the instrument (e.g. extraction method, LAN connection, etc.). Operation Manual FatExtractor E-500 47/88...
The valves close. The heaters switch off. The coolant keeps running 15 min. Navigation control Used for navigating the user interface. Press- ing the control performs the assigned func- tion on the function bar. 48/88 Operation Manual FatExtractor E-500...
The following menus are available: Menu symbol Meaning Submenu/Actions Process control param- [Home] menu eters Editing and saving ex- [Method] menu traction methods Change settings [Configuration] menu Service menu System information Operation Manual FatExtractor E-500 49/88...
On the home menu the following control buttons are available: Button Meaning Skips the actual step. Aborts extraction on the selected extraction position. Editing the running method. 7.3.2 Method menu The [Method] menu allows saving methods. See Chapter 8 "Editing a method", page 54 50/88 Operation Manual FatExtractor E-500...
[Network] Enter value The following parameters can be edited: Device name/DHCP/MAC address/ System IP address/Subnet mask/ Gateway [Delete app con- Confirmation question Resets external connections to the nection] instrument. [Demo Mode] On/Off Simulate an extraction. Operation Manual FatExtractor E-500 51/88...
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Tap up or down. ð Display shows the actual current consumption. Beeper On/Off Chiller On/Off (option) Submenu System Information The submenu [System information] contains details of the connected devices and in- formation on network connection diagnosis. 52/88 Operation Manual FatExtractor E-500...
The solvent menu contains the available solvents for the extraction methods. The solvent menu allows editing individual solvents. See Chapter 10 "Editing a sol- vent", page 72 Status bar The status bar shows actual information about the instrument (e.g. extraction method, LAN connection, etc.). Operation Manual FatExtractor E-500 53/88...
Setting the solvent of a method The following solvents are selectable: Chloroform Diethyl ether Hexane Petroleum ether Own created The control sets the heating level for the selected solvent automatically. 54/88 Operation Manual FatExtractor E-500...
If the extraction time and a number of extraction cycles is set, the extraction will fin- ish once both settings are fulfilled. The number of extraction cycles is selectable from 0 to 5940. Navigation path ➔ Navigate to the [Method] menu via the navigation path. Operation Manual FatExtractor E-500 55/88...
Set the Soxhlet valve opening time so that the Extraction glass chamber Soxhlet is completely emptied. The following open times are available: Open time Level sensor posi- Explanation tion [short] bottom The sample structure is easily releasing the solvent. Hydrolysed sample in the glass sample tubes are extracted. 56/88 Operation Manual FatExtractor E-500...
Setting the rinse heating level of a method (Soxhlet and Hot Extraction only) The default setting for the rinse heating level is depending on the selected solvent. Change the rinse heating level to compensate ambient conditions. Operation Manual FatExtractor E-500 57/88...
Tap the function [Save] on the function bar. ð The value is saved. ð The dialog box closes. 8.11 Setting SmartDrying (Soxhlet and Economic Continuous Extraction only) Smart-Drying automatically sets the parameters for the drying step. 58/88 Operation Manual FatExtractor E-500...
ð The time is saved. 8.13 Setting the drying heating level of a method The default setting for the drying heating level is depending on the selected solvent. Change the drying heating level to compensate fluctuations in ambient conditions. Operation Manual FatExtractor E-500 59/88...
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Select the action [Drying heating level] with the navigation control. Turn the navigation control until the required value. Tap the function [OK] on the function bar. Tap the function [Save] on the function bar. ð The heating level is saved. 60/88 Operation Manual FatExtractor E-500...
Add solvent with a dispenser. Use the support solvent supply (option) Put a support solvent supply on the con- denser. Insert the nozzle of the dispenser into the sup- port solvent supply. Add solvent with a dispenser. Operation Manual FatExtractor E-500 61/88...
Putting the glass sample tube with frit in the extraction glass chamber NOTE Instead of glass sample tube with frit it is possible to insert a thimble. Put the holder for the glass sample tube with frit into the extraction chamber. 62/88 Operation Manual FatExtractor E-500...
ð The instrument is starting up. 9.3.3 Selecting a method Navigation path ➔ Navigate to the [Home] menu via the navigation path. Tap the method. ð The display shows a dialog with the selectable methods. Operation Manual FatExtractor E-500 63/88...
Soxhlet", page 63 Push the chamber rack back in the instrument. Fill the beaker with organic solvent. Put the beaker on the corresponding position on the heating plate. Close the protection shield. 64/88 Operation Manual FatExtractor E-500...
Switch off the connected chiller or close the water tap. Empty the solvent tank. See Chapter 11.2 "Removing the tank bottle", page 74 9.3.7 Shutting down the instrument Navigation path ➔ Precondition: R The extraction process has ended. Switch the On/Off master switch to Off. Operation Manual FatExtractor E-500 65/88...
➔ Navigate to the [Home] menu via the navigation path. Select the extraction position with the navigation control. Tap the function [OK] on the function bar. ð The status of the extraction position is changed. 66/88 Operation Manual FatExtractor E-500...
Risk of dangerous vapors, hot parts and hot glassware. A hot instrument can cause building fire, skin burns or death. Wait 15 min before opening the lift. Do not touch hot parts or surfaces. Use pliers to remove the glassware. Navigation path ➔ Operation Manual FatExtractor E-500 67/88...
ð The display shows a dialog with the selectable methods. Select the method you wish to use. Tap the function [OK] on the function bar. ð The method is selected. 9.5.3 Selecting the extraction positions The extraction positions can show the following status: 68/88 Operation Manual FatExtractor E-500...
ð The lift moves down. Wait until the lift is in the lower position. Make sure that all components of the glass setup are aligned. Testing procedure: Move each condenser a small distance forward and backward. Operation Manual FatExtractor E-500 69/88...
The number of saved QR Codes is limited. 4 When the internal memory is full, the older QR Codes will be overwritten. 9.6.1 Exporting extraction parameters without cloud connection Precondition: R The App is installed on a mobile device. 70/88 Operation Manual FatExtractor E-500...
R The App is installed on a mobile device. R An extraction is finished. R The instrument is connected via LAN cable. See Chapter 5.16 "Mobile connec- tion", page 40 The extraction parameters are automatically transferred to the App. Operation Manual FatExtractor E-500 71/88...
ð The display shows a dialog with an alphanumeric input box. Enter a name for the solvent. Tap the function [Save] on the function bar. ð The new name is saved. ð The dialog box closes. 72/88 Operation Manual FatExtractor E-500...
Any servicing and repair work which involves opening up the casing may only be car- ried out by BUCHI service technicians. Use only genuine BUCHI consumables and spare parts in order to ensure correct operation of the device and preserve the warranty.
Heating plate Wipe down the heating plate with a Weekly damp cloth. If heavily soiled, use ethanol or a mild detergent. If cracked or broken, contact BUCHI customer service Hoses and hose Check the hoses and hose connec- Weekly connections tions for defects (cracks, brittle ar- eas).
Navigate to [Valves] via the navigation path. ð The display shows the view Valves. Select the position of the tank valve you wish to open. Open the tank valve. Wait until all solvent is in the tank bottle. Operation Manual FatExtractor E-500 75/88...
Navigate to [Valves] via the navigation path. ð The display shows the view Valves. Select the position of the Soxhlet valve you wish to open. Open the Soxhlet valve. Wait until the extraction glass chamber is empty. Close Soxhlet valve. 76/88 Operation Manual FatExtractor E-500...
Contact BUCHI Costumer tive (may be the coolant is Service. contaminated with algae, lime, etc.) Protection shield lifted Close the protection shield. Tank bottle full Empty the tank bottle. See Chapter 11.2 "Removing the tank bottle", page 74 Operation Manual FatExtractor E-500 77/88...
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Too long waiting time between Start the instrument quickly preparation of the instrument after solvent was added and start Boiling surface of the beaker Clean the beaker is dirty Boiling surface of the beaker Replace the beaker is worn 78/88 Operation Manual FatExtractor E-500...
Navigate to [Valves] via the navigation path. ð The display shows the view Valves. Select the position of the Soxhlet valve you wish to open. Open the Soxhlet valve. Wait until the extraction glass chamber is empty. Close Soxhlet valve. Operation Manual FatExtractor E-500 79/88...
When disposing, observe the disposal regulations of the materials used. Materials used see Chapter 3.5 "Technical data", page 22 13.3 Returning the instrument Before returning the instrument, contact the BÜCHI Labortechnik AG Service Depart- ment. https://www.buchi.com/contact 80/88 Operation Manual FatExtractor E-500...
Water out Connection Condenser CW OUT Condenser Condenser Flow meter (inside instrument) Condenser Condenser Connection CW Tank OUT Condenser solvent tank CW IN Condenser Connection solvent tank CW Tank IN CW OUT Fig. 16: Condenser circuit Operation Manual FatExtractor E-500 81/88...
Fig. 17: Solvent circuit 14.2 Spare parts and accessories Use only genuine BUCHI consumables and spare parts in order to ensure correct, safe and reliable operation of the system. NOTE Any modifications of spare parts or assemblies are only allowed with the prior written permission of BUCHI.
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Soxhlet assembly cpl. 11067065 One part constiting of magnetic valve and level sensor for extraction glass chamber Soxhlet Protection shield top, cpl. 11067832 Protection shield bottom, cpl. 11067831 Set of seals E-500, FKM, 6 pcs. 11069012 Operation Manual FatExtractor E-500 83/88...
11067479 14.2.2 Conversion and upgrade kits The FatExtractor E-500 can be converted into another configuration with an easy change of glass assembly. Choose the needed conversion kit according to the matrix. Order no. Conversion kit from Soxhlet / ECE to HE 11068487 incl.: 6 beakers HE (3 x 11067475) , beaker brackets HE (2 x 11067829), set...
Upgrade kit Analyte protection 11068524 For later upgrade of a FatExtractor E-500 with analyte protection sensor Upgrade kit Interface Pro 11068525 For later upgrade of a FatExtractor E-500 with a Pro interface (7 " touch screen) 14.2.3 Accessories Order no.
14.2.7 Documentation Order no. IQ/OQ for FatExtractor E-500 11068557 Installation/Operation qualification for the FatExtractor E-500 incl. binder with documents IQ/OQ, inspection and basic tag (English) Repeating OQ for FatExtractor E-500 11068559 Repeating operation qualification for FatExtractor E-500, incl. document, in-...
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