Shodex HILICpak VN-50 1D Operation Manual

Shodex HILICpak VN-50 1D Operation Manual

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(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)

Important Handling Instructions

Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.

Before Using the Column

(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.

1. Introduction

Thank you for purchasing the Shodex product. Shodex HILICpak VN-50 series is a polymer-based hydrophilic
chromatography (HILIC) chlromatography column modified with diol group. It is suitable for separation and
analysis of hydrophilic compounds such as oligosaccharides and oligonucleic acids.

2. Column Components

Please refer to the Shodex website: https://www.shodex.com/en/da/07.html

3. Column Specifications

Product
Product Name
Code
F7630800
HILICpak VN-50 1D
Base Material
: Spherical porous particles of polyvinyl alcohol modified with diol group
Column Housing : Double structure with outer surface SUS304N2 and inner surface (wetted part) PEEK
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile = 25/75

4. Usable Conditions

Product Name
HILICpak VN-50 1D
Operation Manual
Shodex HILICpak VN-50 1D
Column Size (mm)
I.D.
1.0
Flow Rate (mL/min)
Recommended
Maximum
0.01 - 0.05
Resonac Corporation (https://www.shodex.com/)
Particle Size
(μm)
Length
150
5
Maximum
Pressure
(MPa/column)
0.1
10
ver.20230322E
Theoretical Plate Number
(Per Column)
≥ 2,800
pH
Temperature
Range
Range (°C)
2 - 13
4 - 60
1

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Summary of Contents for Shodex HILICpak VN-50 1D

  • Page 1 Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number. 1. Introduction Thank you for purchasing the Shodex product. Shodex HILICpak VN-50 series is a polymer-based hydrophilic chromatography (HILIC) chlromatography column modified with diol group. It is suitable for separation and analysis of hydrophilic compounds such as oligosaccharides and oligonucleic acids.
  • Page 2 · Full column performance may not be achieved if a sample concentration is higher or sample injection volume is larger than it should be. It may lead to abnormal peak shapes, poor separations, and/or low reproducibility. In such cases, please adjust the sample concentration and/or the injection volume. Resonac Corporation (https://www.shodex.com/)
  • Page 3 7. Column Usage Procedure HILICpak VN-50 1D is a semi-micro column. To achieve its best performance, please use it with a semi- micro type HPLC system. 7.1 HPLC System Preparation Wash the entire HPLC system prior to column installation, including all flow-lines and sample loop by switching the valves, and then replace the washing solution with the eluent to be used.
  • Page 4 Securely tighten the end caps and store the column at a location with stable temperature (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside the column to dry. It can damage the column. Resonac Corporation (https://www.shodex.com/)
  • Page 5 9. Column Inspection Please refer to the inspection method described in the CoA. At Shodex, “half width method” is adopted for the calculation of plate count and “asymmetry factor (Fas)” is adopted for the calculation of peak symmetry. Please refer to the Shodex website for the detail: https://www.shodex.com/en/da/07.html...

This manual is also suitable for:

F7630800

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