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（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column adhesive label, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex product. Shodex HILICpak VN-50 series is a polymer-based HILIC
column modified with diol group. The column is suitable for the analysis of hydrophilic compounds such as
oligosaccharides and oligonucleic acids.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6830100
HILICpak VN-50 10E
F6711400
HILICpak VN-50G 4A
Base Material
: Spherical porous particles of polyvinyl alcohol modified with diol group
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile = 25/75
Operation Manual
Shodex
HILICpak
Column Size (mm)
I.D.
10.0
4.6
SHOWA DENKO K.K. (https://www.shodex.com/)
VN-50 10E
Particle
Size (μm)
Length
250
5
10
5
ver.211105E
Theoretical Plate Number
(Per Column)
≥ 11,000
(Guard Column)
1
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Summary of Contents for Shodex HILICpak VN-50 10E
- Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex HILICpak VN-50 series is a polymer-based HILIC column modified with diol group. The column is suitable for the analysis of hydrophilic compounds such as oligosaccharides and oligonucleic acids.
- Page 2 Pressure (MPa) pH Range Range (°C) Recommended Maximum (Per Column) HILICpak VN-50 10E 2 - 3 2 - 13 4 - 60 HILICpak VN-50G 4A Usable solvents are listed below. (1) A mixture of water and acetonitrile or water and methanol of any ratio can be used. Up to 100 % water, acetonitrile, or methanol can be used.
- Page 3 This may result in creating an empty space in the column and deteriorates the column. Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 4 (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
- Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.
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