Functionality For Emission Fingerprinting - Zeiss LSM 510 Manual

LSM 510
LSM 510 META
Linear Unmixing button
Apply to hardware button
Save to spectra DB button
4.13.28.4 Functionality for Emission Finger-
printing
Extract Channels panel
− Sum signals from selected detector elements
− Mean
signals
elements
− Autoscaling of output channels (individually)
Linear Unmixing panel
− Autoscaling of output channels
− Generate an additional "Channel with
Residuals"-> pixel-by-pixel display of the
difference between fit and original data (for
the channel of the Lambda Stack that shows
the greatest deviation)
− Advanced Linear Unmixing ignored negative
results and repeats the calculation.
− Ask for ROI characterizing background
spectra before Linear Unmixing
− Ignore
Overexposed
Pixels. Those wont be considered for the
calculation.
Context menu
− Spectra Display with option Normalize
(right mouse click into spectra graph)
03/06
OPERATION IN EXPERT MODE
Display and Analysis of Images
Performs the unmixing with the selected spectra.
Sets the parameters for the META detector used in binning mode as
specified in the settings ChS1, ChS2, ChS3 ... ChS8. See Single Track
and Multi Track in Config Control for the effect of the action. The
Lambda Stack setting is not affected by this action.
The values of the intensity Lambda diagram are saved in a file
(extension: *.rls) in the aim/macro/roi directory. Make sure to have
displayed only data of single dyes in the intensity Lambda diagram when
executing this function.
from selected detector
and Underexposed
Fig. 4-316
Fig. 4-317
B 45-0021 e
Extract and Unmix Properties window
Context menu with Normalize function
Carl Zeiss
4-341