Labeling Mode - Lifecanvas Technologies SmartBatch+ User Manual

23. Remove Sample Ring from the Clearing Cup and wash samples with PBSN (PBS with
0.02% sodium azide) in the Incubation Jar. Carefully rinse the Clearing Cup with distilled
water and store it in its storage solution. It is important to keep the membrane hydrated
at all times.
24. When done clearing samples, or every 10 days (whichever comes first) wash the system
and replace the buffer in the device. We recommend refreshing the Delipidation Buffer
when adding new samples, or every ~3 days, whichever comes first.
25. Wash the device before turning it off to prevent detergent buildup.

Labeling Mode

1. Wash the device and turn off the Auxiliary Power.
2. Drain out any liquid from the device and ensure the Drainage Valve is closed. Soak up
any remaining liquid from the Chamber using a paper towel.
3. Pour a whole bottle of SmartBatch+ Primary Device Buffer into the Chamber.
4. Remove the Batch Staining Cup from its storage container, and wash carefully with a
gentle stream of water. (If you are running a single sample experiment use the Single
Sample Staining Cup instead).
5. Place the Batch Staining Cup into a beaker of distilled water to finish washing it.
6. Remove the Batch Staining Cup from the liquid and dump out any water. Carefully use a
kimwipe to soak up any remaining water inside the cup.
7. Rinse out the cup twice with Primary Sample Buffer.
8. Fill the cup with 40mL of Primary Sample Buffer and add the appropriate molecular
probes.
9. Insert the Sample Ring into the Batch Staining Cup with the samples on their Mesh
Bags. Ensure samples are properly pre-incubated.
10. Insert the cup in the Chamber, lining up the pegs on the bottom with those in the
Chamber Hex Piece. The cup will fit in snugly.
11. Turn on the Auxiliary Power. The buffer should begin to flow and the stirrer should
begin spinning. The cup will initially rotate relatively quickly but will slow down prior to
the experimental run.
12. Close and seal the Chamber Lid and the Case Lid.
13. Press "Preset" until it indicates Labeling 1 or Labeling 2 Mode for primary and
secondary antibodies accordingly. Change any settings or apply the timer if desired. The
preset settings are the recommended settings.
14. From here, please follow the instructions in the Labeling section of the Full Pipeline
Protocol to finish the experiment.
www.lifecanvastech.com info@lifecanvastech.com
13