Thermo Scientific Dionex ICS-2000 Operator's Manual
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Thermo Scientific Dionex ICS-2000 Operator's Manual

Thermo Scientific Dionex ICS-2000 Operator's Manual

Ion chromatography system
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ICS-2000 ION CHROMATOGRAPHY SYSTEM
OPERATOR'S MANUAL
Document No. 031857
Revision 03
April 2006

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Summary of Contents for Thermo Scientific Dionex ICS-2000

  • Page 1 ICS-2000 ION CHROMATOGRAPHY SYSTEM OPERATOR’S MANUAL Document No. 031857 Revision 03 April 2006...
  • Page 2 ©2006 by Dionex Corporation All rights reserved worldwide. Printed in the United States of America. This publication is protected by federal copyright law. No part of this publication may be copied or distributed, transmitted, transcribed, stored in a retrieval system, or transmitted into any human or computer language, in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties without the express written permission of Dionex Corporation, 1228 Titan Way,...

  • Page 3: Table Of Contents

    Contents 1 • Introduction Introduction to Ion Chromatography (IC) ..... . .1-1 Overview of the ICS-2000 ........1-4 About This Manual .
  • Page 4 ICS-2000 Ion Chromatography System 2.4.4 Eluent Generator ........2-35 2.4.5 Injection Valve .
  • Page 5 Contents 3.10.1 Loading Samples with a Syringe ..... .3-15 3.10.2 Loading Samples with a Vacuum Syringe ....3-16 3.10.3 Loading Samples with an Autosampler .
  • Page 6 ICS-2000 Ion Chromatography System 4.13 Baseline Noise or Drift ........4-22 4.14 Vacuum Degas Assembly Does Not Run .
  • Page 7 Contents 5.16 Changing Main Power Fuses ....... .5-37 5.17 Replacing an EluGen Cartridge ......5-38 5.17.1 Replacing a KOH, LiOH, MSA, or NaOH EluGen Cartridge .
  • Page 8 ICS-2000 Ion Chromatography System Physical ..........A-1 Environmental .
  • Page 9 Contents B.8.1 Assigning the ICS-2000 to a Timebase....B-15 B.8.2 Assigning DX-LAN Devices to the Timebase (Optional) . . B-20 Installing and Plumbing the Columns and Suppressor ... B-23 B.9.1 Setting Up the Column Heater .
  • Page 10 ICS-2000 Ion Chromatography System B.18.2 AS or AS50 (USB) Chromeleon Setup ....B-110 B.19 Connecting an AS40 Automated Sampler (Optional) ...B-111 B.20 Connecting the Analog Output (Optional) .
  • Page 11 Contents C.6.2 Status Parameter Details ......C-15 Pump Page ..........C-16 C.7.1 Setting Pump Pressure Limits and Selecting the Pressure Unit .
  • Page 12 ICS-2000 Ion Chromatography System Controlling TTL and Relay Outputs ......D-5 Example Setup for Stand-Alone Operation .....D-7 E •...

  • Page 13: Introduction

    1 • Introduction Introduction to Ion Chromatography (IC) The Dionex ICS-2000 Ion Chromatography System (ICS-2000) performs ion analyses using suppressed or non-suppressed conductivity detection. An ion chromatography system typically consists of a liquid eluent, a high-pressure pump, a sample injector, a guard and separator column, a chemical suppressor, a conductivity cell, and a data collection system.
  • Page 14 ICS-2000 Ion Chromatography System A typical IC analysis consists of six stages (see Figure 1-1). 6. Data Analysis Conductivity 5. Detection Cell Suppressor 4. Suppression Eluent 1. Eluent Separator Delivery Column 3. Separation Pump Guard Column Injection Valve Sample 2. Sample Injection Sample Loop Figure 1-1.
  • Page 15 1 • Introduction • The pump pushes the eluent and sample through the guard and separator columns (chemically-inert tubes packed with a polymeric resin). The guard column removes contaminants that might poison the separator column. 3. Separation • As the eluent and sample are pumped through the separator column, the sample ions are separated.

  • Page 16: Overview Of The Ics-2000

    ICS-2000 Ion Chromatography System Overview of the ICS-2000 The ICS-2000 is an integrated ion chromatography system containing an eluent generator, pump, injection valve, column heater, and conductivity detector. Other system components, including a guard column, separator column, and suppressor vary, depending on the analyses to be performed. The ICS-2000 is also configured with a vacuum degas assembly, for online eluent degassing.

  • Page 17: About This Manual

    1 • Introduction About This Manual The electronic version (i.e., PDF file) of the ICS-2000 operator’s manual contains numerous hypertext links that can take you to other locations within the file. These links include: • Table of contents entries • Index entries •...

  • Page 18: Safety Messages And Notes

    ICS-2000 Ion Chromatography System Appendix D Describes the ICS-2000 TTL and relay control features. TTL and Relay Control Appendix E Lists spare parts for the ICS-2000. Reordering Information Appendix F Provides answers to frequently asked questions about ICS- 2000 operation. Appendix G Defines terms commonly used in ion analysis.
  • Page 19 1 • Introduction Messages d'avertissement en français Signale une situation de danger immédiat qui, si elle n'est pas évitée, entraînera des blessures graves à mortelles. Signale une situation de danger potentiel qui, si elle n'est pas évitée, pourrait entraîner des blessures graves à mortelles. Signale une situation de danger potentiel qui, si elle n'est pas évitée, pourrait entraîner des blessures mineures à...

  • Page 20: Safety Labels

    ICS-2000 Ion Chromatography System 1.3.2 Safety Labels The TUV GS and c TUVus Mark safety labels and the CE Mark label on the ICS-2000 indicate that the ICS-2000 is in compliance with the following standards: EN 61010-1:2001 (safety), CAN/CSA-C22.2 No. 1010.1-92 +A2:97 (safety), UL 3101-1/10.93 (safety), and EN 61326:1997, including A1:1998 and A2:2001 (EMC susceptibility and immunity).

  • Page 21: Features

    2 • Features This chapter describes key ICS-2000 features and introduces the Chromeleon user interface. Operating Features 2.1.1 Front Panel Figure 2-1 illustrates the front panel of the ICS-2000. Injection Port The sample to be analyzed can be injected manually into the injection port, using a syringe.
  • Page 22 ICS-2000 Ion Chromatography System Summary of Touch Screen Operation Status Field Edit Field Command Button (selected) Command Button (unselected) Figure 2-2. ICS-2000 Touch Screen Home Page NOTE To adjust the screen contrast, open the front door and adjust the knurled knob under the screen (see Figure 2-8).
  • Page 23 2 • Features • Selecting a command button changes the button’s appearance to white text on a dark backgound (for example, • Touching an edit field (for example, ) opens a page with a number keypad (see Figure 2-3). Use the keypad to enter the desired numerical value for the field and then touch the ENTER button.
  • Page 24 ICS-2000 Ion Chromatography System • Touching the page name (for example, ) in the bottom right corner opens a menu of pages (see Figure 2-4). Touch a page name to display the page. Figure 2-4. ICS-2000 Touch Screen Menu of Pages •...
  • Page 25 2 • Features page (see Figure 2-5). See Appendix C for details SUPPRESSOR about each touch screen page. Touch to select a different option. Touch to return to the Home page. Figure 2-5. Suppressor Page • Touching returns you to the page.

  • Page 26: Top Cover

    ICS-2000 Ion Chromatography System 2.1.2 Top Cover Figure 2-7 illustrates the top cover of the ICS-2000. EluGen Cartridge and Reservoir EluGen Storage Cartridge The ICS-2000 top cover holds and Holder one or two EluGen cartridges ® Second (see below) and up to two 2-L EluGen plastic reservoirs (P/N 044129).

  • Page 27: Component Panel

    2 • Features EGC Service Area The EGC service area holds the cartridge during installation and replacement. See Section B.14 for instructions. 2.1.3 Component Panel Figure 2-8 shows the user-accessible components installed on the component panel behind the ICS-2000 front door. Eluent Valve Leak Sensor Pump Heads...
  • Page 28 ICS-2000 Ion Chromatography System Eluent Valve The eluent valve controls the flow from the eluent reservoir. The eluent valve opens automatically when the pump is started and closes when the pump is turned off. Leak Sensor The leak sensor is installed in the drip tray at the bottom of the component panel.
  • Page 29 2 • Features Suppressor The suppressor reduces the eluent conductivity and enhances the conductivity of the sample ions, thereby increasing detection sensitivity. Either an AES® Atlas Electrolytic Suppressor, SRS® Self-Regenerating Suppressor, or MMS™ MicroMembrane™ Suppressor can be used with the ICS-2000. See Section 2.4.7 for details about the suppressor.

  • Page 30: Rear Panel

    ICS-2000 Ion Chromatography System 2.1.4 Rear Panel Figure 2-9 illustrates the ICS-2000 rear panel. Tubing Clips Analog Output Connector Power Switch Connectors TTL and Relay Main Power Connector Strip Receptacle Waste Lines Figure 2-9. ICS-2000 Rear Panel Analog Output Connector The analog output connector outputs conductivity data (scaled to 1 volt) to an integrator or recording device.
  • Page 31 2 • Features TTL and Relay Connector The TTL and Relay connector strip provides two TTL outputs, two relay outputs, and four TTL inputs. The outputs can be used to control functions in other TTL- or relay-controllable devices. The inputs can be used to switch the injection valve position, turn on the pump, perform an autozero command, and send an event mark to the analog output.

  • Page 32: The Chromeleon Interface

    ICS-2000 Ion Chromatography System The Chromeleon Interface NOTE This section provides a brief overview of the Chromeleon interface. For details, refer to the Chromeleon Help or user’s manual. Chromeleon software is used for remote control of ICS-2000 operation. Two modes of software control are available: direct control and automated control. •...

  • Page 33: The Ics-2000 Control Panels

    2 • Features NOTE The Chromeleon Browser looks and functions like Windows Explorer, but do not confuse the two. If you copy files from the Chromeleon Browser into Explorer, the files will be corrupted. The Browser displays the directories in which chromatographic data is located, and allows you to open, move, and delete data.
  • Page 34 ICS-2000 Ion Chromatography System Figure 2-11. ICS-2000 System Control Panel (Traditional) Figure 2-12. ICS-2000 System Control Panel (Graphical) 2-14 Doc. 031857-03 4/06...
  • Page 35 2 • Features System Controls Overview This section provides brief descriptions of the various controls available on the ICS-2000 System Control panels. Sample Controls Datasource Displays the database in which the chromatographic data for the sample is saved. Sequence Displays the sequence in which this sample is included. Sample Displays the name of the sample that is running.
  • Page 36 ICS-2000 Ion Chromatography System Audit Trail The Audit Trail displays an account of every event that occurs during ICS-2000 operation. This includes errors, status messages, operational events, etc. System Controls Connected Connects the ICS-2000 to the Chromeleon Control panel, enabling communication between the PC and the module.
  • Page 37 2 • Features Pump Controls Flow Rate Sets the pump flow rate in mL/min. Eluent Bottle The entry field and gauge indicate the level of liquid (in liters) in the eluent bottle. This is a user-controlled feature. The level must be entered by the user when the reservoir is filled.
  • Page 38 ICS-2000 Ion Chromatography System Injection Valve Controls LoadPosition InjectPosition Displays the current position of the injection valve. Load Switches the valve to the load position. Inject Switches the valve to the inject position. Injection Valve Switch Switches the valve from the current position to the other position. Column Heater Controls Temperature Displays the current heater temperature and lets you set a new...
  • Page 39 2 • Features Detector Controls Autozero Sets the conductivity to zero. Acq. Ready Indicates whether the detector is ready to begin data acquisition. Total Cond Displays the total conductivity (the conductivity including the offset conductivity). To determine the offset, allow the system to equilibrate after startup.
  • Page 40 ICS-2000 Ion Chromatography System Signal Plot Displays a plot of the detector signal. Right-click to select plot options from a context menu. You can select autoscale commands, change the plot appearance, or select other signals to be displayed (for example, total conductivity or pump pressure).
  • Page 41 2 • Features Exp. Date Displays the expiration date of the installed cartridge. Although you can continue operation with the cartridge after the expiration date, performance may be impaired until a new cartridge is installed. Cartridge Type Displays the type of cartridge installed. Remaining Life Displays the percentage of ions remaining in the cartridge.
  • Page 42 ICS-2000 Ion Chromatography System controls for each cartridge in the dual panel are the same as described for the single cartridge panel controls (see page 20). Target Concentration Sets the concentration of eluent generated. If a K EluGen cartridge and an EPM Electrolytic pH Modifier are installed (connected to EGC-1 , respectively), set the EGC-1 Target Concentration to the EGC-2...

  • Page 43: Flow Schematics

    2 • Features Flow Schematics The following figures illustrate the liquid flow path through an ICS-2000 when using suppression in autorecycle mode. Three configurations are shown: • Figure 2-13 illustrates the liquid flow path when the components required for producing KOH, LiOH, MSA, or NaOH eluent are installed. The required components include the corresponding type of EluGen cartridge and a CR- •...
  • Page 44 ICS-2000 Ion Chromatography System To the pump Degas In EGC Vent EGC II KOH, LiOH, MSA, or NaOH Deionized Water Inlet Outlet Degas CR-TC From the pulse damper Degas In To the Column inject valve Heater port P(2) Degas Out Conductivity Cell Suppressor...
  • Page 45 2 • Features Flow Description for KOH, LiOH, MSA, or NaOH Eluent Generation Refer to Figure 2-13 for the flow path number locations. • Deionized water from the reservoir flows first through the pump degas assembly and then through the eluent valve to the pump .
  • Page 46 ICS-2000 Ion Chromatography System To the pump Degas In EGC Vent EGC II Deionized Water Inlet Outlet Eluent Out Regen Out Eluent In Degas Regen In Degas In Column Heater Degas Out Conductivity Cell Suppressor Cell In Cell Out Pulse Damper Valve Press.
  • Page 47 2 • Features Flow Description for Carbonate/Bicarbonate Eluent Generation Refer to Figure 2-14 for the flow path number locations. • Deionized water from the reservoir flows first through the pump degas assembly and then through the eluent valve to the pump . The water is then pushed through the pressure transducer , which measures the system pressure.
  • Page 48 ICS-2000 Ion Chromatography System To the pump Degas In EGC Vent EGC II Deionized Water Inlet Outlet Degas Degas In Column Heater Degas Out Conductivity Cell Suppressor Cell In Cell Out Pulse Damper Valve Press. Xducer. To waste Pump Pump Head Head To Gas...
  • Page 49 2 • Features Flow Description for Carbonate Eluent Generation Refer to Figure 2-15 for the flow path number locations. • Deionized water from the reservoir flows first through the pump degas assembly and then through the eluent valve to the pump . The water is then pushed through the pressure transducer , which measures the system pressure.

  • Page 50: System Component Details

    ICS-2000 Ion Chromatography System System Component Details This section provides details about ICS-2000 system components, including the vacuum degas assembly, pump, eluent generator, injection valve, column heater, suppressor, and conductivity cell. 2.4.1 Vacuum Degas Assembly The vacuum degas assembly provides online eluent degassing at the time and duration specified by the user.
  • Page 51 2 • Features under the timebase, and select Edit>Properties. Select the Options tab (see Figure 2-16). Figure 2-16. Server Configuration Properties: Degas Mode Options Degas Mode Options • : The degas pump is always off. Always Off • Always On : The degas pump is always on.

  • Page 52: Eluent Valve

    ICS-2000 Ion Chromatography System 2.4.2 Eluent Valve The eluent valve controls the flow from the eluent reservoir. The valve opens automatically when the pump is started and closes when the pump is turned off. The valve can also be opened and closed manually either from the Chromeleon Control panel (see Section...
  • Page 53 2 • Features Outlet Check Pressure Valve Transducer Primary Pump Head Secondary Pump Head Priming Valve Inlet Check Waste Valve Valve Figure 2-18. ICS-2000 Pump Components To open the priming valve, turn the knob one-quarter to one-half turn counterclockwise. When the priming valve is open, liquid can flow into and out of the primary pump head via the port in the front of the valve.
  • Page 54 ICS-2000 Ion Chromatography System Pressure Transducer Flow exiting the secondary pump head is directed to the pressure transducer (see Figure 2-18), which measures the system pressure. Pressure readings indicate that the pumping system is delivering smooth, accurate flow. Pressure readings can be monitored from the Chromeleon Control panel or the touch screen page.

  • Page 55: Eluent Generator

    2 • Features 2.4.4 Eluent Generator The eluent generator produces high-purity eluents online, using only deionized water as the carrier. The eluent generator consists of an eluent generator cartridge (EGC) that generates the eluent, and a high-pressure degas tubing assembly that removes electrolysis gases created during eluent generation.
  • Page 56 ICS-2000 Ion Chromatography System You can select the concentration of eluent to be generated from either the Chromeleon Control panel or from the touch screen page (see HOME Section C.4). The allowable eluent concentration depends on several factors: the flow rate, suppressor type, EluGen cartridge type, and cartridge configuration.
  • Page 57 2 • Features details, refer to the EluGen cartridge manual. Cartridge manuals are included on the Dionex Reference Library CD-ROM (P/N 053891). EluGen Eluent Concentration Range Comment Cartridges /EPM 0.1 to 15 mM at 0.1 to 1.0 mL/min The total of the eluent flow concentrations from Electrolytic...
  • Page 58 ICS-2000 Ion Chromatography System only one cartridge can be installed, because the pH Modifier must be connected to the other EGC power supply ( EGC-1 EGC-2 Tubing and fittings for plumbing the cartridge, degas assembly, CR-TC, and EPM are included with the holder. See Section B.14 for installation instructions.
  • Page 59 2 • Features EPM Electrolytic pH Modifier and EGC CO Mixer The EGC II K EluGen Cartridge can be used with an EluGen EPM Electrolytic pH Modifier Cartridge (P/N 063175) (see Figure 2-19) and an EGC CO Mixer (P/N 061686) to produce a carbonate/ bicarbonate mixture.

  • Page 60: Injection Valve

    ICS-2000 Ion Chromatography System 2.4.5 Injection Valve The injection valve (P/N 057968) is a six-port, electrically-activated valve. A 25-µL sample loop (P/N 042857) is installed on the valve at the factory. The valve has two operating positions: Load and Inject (see Figure 2-20).
  • Page 61 2 • Features sample loops in various sizes. If necessary, the pre-installed 25-µL loop can be replaced with a loop that has a different sample injection volume. Figure 2-21. Injection Valve Plumbing NOTE The EluGen cartridge requires at least 14 MPa (2000 psi) of system backpressure for removal of electrolysis gas from the eluent produced by the cartridge.

  • Page 62: Column Heater

    ICS-2000 Ion Chromatography System 2.4.6 Column Heater The column heater provides temperature control for the separator and guard column. The heater temperature can be set to between 30 and 60 °C. However, the set temperature must be at least 5 °C above the ambient temperature.

  • Page 63: Ds6 Heated Conductivity Cell

    2 • Features 2.4.8 DS6 Heated Conductivity Cell The ICS-2000 flow-through conductivity cell measures the electrical conductance of analyte ions as they pass through the cell. Two passivated 316 stainless steel electrodes are permanently sealed into the PEEK cell µ body.
  • Page 64 ICS-2000 Ion Chromatography System 2-44 Doc. 031857-03 4/06...

  • Page 65: Operation And Maintenance

    3 • Operation and Maintenance This chapter describes routine operating and maintenance procedures for the ICS- 2000 Ion Chromatography System. The ICS-2000 is designed for IC (ion chromatography) applications and should not be used for any other purpose. Operation of the ICS-2000 in a manner not specified by Dionex may result in personal injury.
  • Page 66 ICS-2000 Ion Chromatography System Sample Processing Overview Samples can be run manually (one at a time), or they can be grouped and run automatically in batches. Figure 3-2 shows the typical steps for manual and batch sample processing. Manual Sample Manual Sample Batch Sample Processing...

  • Page 67: Power Up The System

    3 • Operation and Maintenance Power Up the System Press the power switch on the ICS- 2000 rear panel (see Figure 3-3) to turn on the system power. Table 3-2 shows the ICS-2000 conditions at Power Switch power up. Also turn on the power to the computer and the autosampler (if used).

  • Page 68: Start Chromeleon

    ICS-2000 Ion Chromatography System Start Chromeleon NOTE If you are beginning operation of an ICS-2000 that has not been configured in a timebase, see Section B.8 Chromeleon setup instructions. 1. If the Chromeleon Server is not running (the icon on the taskbar is crossed out in red ), start the Server by right-clicking the icon and selecting Start Server.
  • Page 69 3 • Operation and Maintenance 3. In the Browser, expand the Dionex Templates\Panels\Dionex_IC\ICS-2000 System Panels folder. Several ICS-2000 Control panels (.PAN files), corresponding to various system configurations, are provided. Two styles of panels are available: • Traditional panels provide controls on a single window with minimal graphics.

  • Page 70: Set Up The Eluent Reservoir

    ICS-2000 Ion Chromatography System Figure 3-6 shows a connected Control panel for an ICS-2000 system. Figure 3-6. ICS-2000 System Control Panel (Connected) Set Up the Eluent Reservoir 3.4.1 Filter the Deionized Water Filtering removes small particulates in the deionized water that may contaminate the pump check valves and cause erratic flow rates or loss of prime.

  • Page 71: Fill The Reservoir

    3 • Operation and Maintenance 3.4.2 Fill the Reservoir Fill the reservoir with ASTM filtered, Type I (18-megohm) deionized water. 3.4.3 Set the Eluent Level After filling the reservoir, enter the volume of liquid in the reservoir in one of the following locations: •...
  • Page 72 ICS-2000 Ion Chromatography System page (see Figure 3-9) also displays the amount of liquid STATUS remaining in the reservoir and an estimate of how many hours remain before the eluent reservoir is empty. See Section C.6 for details about the STATUS PAGE Figure 3-9.

  • Page 73: Connect The Reservoir

    3 • Operation and Maintenance 3.4.4 Connect the Reservoir If it is not already connected, connect the ELUENT BOTTLE line from the reservoir ELUENT cap to the line, ELUENT IN BOTTLE which extends from the plumbing and cable chase on the top of the ICS-2000 (see Figure 3-10).

  • Page 74: Prime The Pump

    ICS-2000 Ion Chromatography System Prime the Pump If you changed eluent or if the eluent lines are dry, prime the lines first with a syringe before following the procedure below to prime the pump. See Section B.13 for instructions on priming the lines with a syringe.

  • Page 75: Set System Operating Conditions

    3 • Operation and Maintenance Set System Operating Conditions NOTE This section is an overview of the steps needed to start up the system and begin running samples. Actual operating parameters (flow rate, cell heater temperature, suppressor current, etc.) depend on the application to be run.

  • Page 76: Equilibrate The System And Verify Operational Status

    ICS-2000 Ion Chromatography System NOTE The suppressor, eluent generator, and CR-TC are always turned off automatically when the pump is turned off. Equilibrate the System and Verify Operational Status NOTE You can monitor system functions (conductivity, pressure, cell temperature, etc.) from the Chromeleon Control panel or from the touch screen page.

  • Page 77: Prepare Samples

    3 • Operation and Maintenance readings on the Control panel are the same, or when the “ symbol is =” displayed next to the temperature on the page. Column Heater HOME 7. Verify that the column heater temperature is at the set point and stable. The temperature is at equilibrium when the Set Temperature and the Current Temperature readings on the Control panel are the same, or when the “...

  • Page 78: Diluting Samples

    ICS-2000 Ion Chromatography System Filter groundwater and wastewater samples through 0.45 µm filters before injection, unless samples were filtered after collection. Before injection, pretreat samples that may contain high concentrations of interfering substances by putting them through Dionex OnGuard ® cartridges.

  • Page 79: Loading Samples With A Syringe

    3 • Operation and Maintenance Setup for an AS40 Autosampler A relay or TTL connection is required for Chromeleon to control sample loading. Section B.19 for AS40 autosampler installation instructions. Setup for an AS or AS50 (USB) Autosampler To use an AS or AS50 (USB) autosampler with the ICS-2000, the following requirements must met: •...

  • Page 80: Loading Samples With A Vacuum Syringe

    ICS-2000 Ion Chromatography System 2. Fill the 1 cc syringe (P/N 016388) provided in the ICS-2000 Ship Kit (P/N 064375) with a calibration standard or sample. 3. Insert the syringe into the injection port on the ICS-2000 front door. 4. Verify that the injection valve is in the Load position. 5.

  • Page 81: Injecting Samples

    3 • Operation and Maintenance • Enter the commands for loading the sample on the autosampler front panel. 4. Switch the injection valve to the Inject position (see Section 3.10.4). 3.10.4 Injecting Samples After loading the sample in the sample loop, use one of the following methods to switch the injection valve to the Inject position: •...

  • Page 82: Process Samples

    ICS-2000 Ion Chromatography System Example AS40 Program Commands -2.300 Pump_ECD_Relay_1.Closed Duration=138.00 ;Note 1 0.000 Pump_ECD.Autozero ;Note 2 ECD_1.AcqOn ;Note 3 Pump_InjectValve.InjectPosition Duration=30.00 ;Note 4 30.00 ECD_1.AcqOff ;Note 5 AS40 Program Command Notes: 1. Close the ICS-2000 , which is connected to the AS40 RELAY OUT 1 relay.

  • Page 83: Automatic (Batch) Sample Processing

    3 • Operation and Maintenance 1. Complete the steps in Sections through to prepare for operation and to start up the ICS-2000. 2. Load the sample, using a syringe, vacuum syringe, or autosampler (see Section 3.10). 3. In Chromeleon, select Control>Acquisition On. Chromeleon records the signal supplied by the detector and displays the signal plot on the Control panel.
  • Page 84 ICS-2000 Ion Chromatography System 1. Complete the steps in Sections through to prepare for operation and to start up the ICS-2000. 2. Create a new sequence or modify an existing one. To create a new sequence: a. In the Browser, select File>New. Select Sequence (using Wizard) and click OK.

  • Page 85: Maintenance

    3 • Operation and Maintenance 4. Click the Add button on the Batch List tab and add the sequence you created in Step 2. See Figure 3-14 for an example. Figure 3-14. Batch Dialog Box with an Example Sequence Added to the Batch List 5.
  • Page 86 ICS-2000 Ion Chromatography System Weekly • Once a week, check fluid lines for crimping or discoloration. Relocate any pinched lines. Replace damaged lines. • Check the junctions between the pump heads and the pump casting for evidence of liquid leaks. If the piston seal wash tubing is not connected, check the drain tubes at the rear of the pump heads for evidence of moisture.

  • Page 87: Troubleshooting

    4 • Troubleshooting This chapter is a guide to troubleshooting minor issues that may arise during operation of the ICS-2000. Turn to the section of this chapter that best describes the operating problem or symptom that has been observed. Each section lists possible causes of the problem or symptom in order of probability.
  • Page 88 ICS-2000 Ion Chromatography System The table below lists the most frequently observed ICS-2000 error messages. For troubleshooting assistance, refer to the page indicated in the table. Alarms and Error Conditions Column heater exceeds safe temperature page 3 Column heater open circuit page 3 Column heater short circuit page 4...

  • Page 89: Troubleshooting Error Messages

    4 • Troubleshooting Alarms and Error Conditions Suppressor over current page 10 Suppressor over voltage page 11 Suppressor over power page 11 Suppressor not connected page 11 Suppressor stopped for flow rate page 12 Table 4-4. ICS-2000 Alarms and Error Messages Summary (Continued) Troubleshooting Error Messages COLUMN HEATER EXCEEDS SAFE TEMPERATURE This error occurs when the column heater temperature is higher than the...
  • Page 90 ICS-2000 Ion Chromatography System COLUMN HEATER SHORT CIRCUIT This error occurs when there is a short circuit on the thermistor input used to measure the column heater temperature. This error indicates a faulty column heater. To troubleshoot: Replace the column heater (see Section 5.11).
  • Page 91 4 • Troubleshooting To troubleshoot: 1. Retry the calibration. 2. If calibration still fails, the vacuum degas assembly may need to be replaced. Contact Dionex for assistance. EGC BOARD NOT PRESENT This error occurs if Chromeleon sends a command to the ICS-2000 related to the eluent generator, but the eluent generator controller board is either not installed or the ICS-2000 does not recognize the installed board.
  • Page 92 ICS-2000 Ion Chromatography System To troubleshoot: 1. Verify that the eluent generator cartridge is connected to the EGC-1 connector on top of the ICS-2000 (see Figure 2-7). 2. If the error persists, contact Dionex for assistance. There may be a malfunction in the EGC control electronics.
  • Page 93 4 • Troubleshooting EGC-1 OVER-CURRENT EGC-2 OVER-CURRENT This error occurs when the current applied to the eluent generator cartridge exceeds the maximum current allowed. Under these conditions, the EGC current is turned off to prevent damage to the EGC. This error may occur if the liquid flow to the cartridge is interrupted.
  • Page 94 ICS-2000 Ion Chromatography System To troubleshoot: 1. Locate the source of the leak by visually inspecting the tubing, fittings, and components on the component panel. 2. Tighten fittings or replace tubing and fittings as required. Refer to Section 4.2 for detailed troubleshooting of various types of leaks. 3.
  • Page 95 4 • Troubleshooting PRESSURE SLOPE CALIBRATION ERROR This error occurs if you try to calibrate the pressure slope when the pressure is less than 3 MPa (500 psi). To troubleshoot: • Contact Dionex for assistance PUMP MOTOR LOST CONTROL This error indicates a problem in the pump controller electronics. To troubleshoot: •...
  • Page 96 ICS-2000 Ion Chromatography System 3. Check that the high pressure limit is set to the correct value. 4. Restart the pump. PUMP UNDER PRESSURE If the system pressure falls below the low pressure limit, the pump stops and this error message appears. Pressure limits can be set in the Chromeleon Server Configuration or in the control program.
  • Page 97 4 • Troubleshooting SUPPRESSOR OVER VOLTAGE This error occurs if you turn on the suppressor but the ICS-2000 cannot establish a connection with the suppressor. To troubleshoot: 1. Check the suppressor cable connection (see Section 5.10). 2. If the error persists, replace the suppressor (see Section 5.10).

  • Page 98: Liquid Leaks

    ICS-2000 Ion Chromatography System SUPPRESSOR STOPPED FOR ZERO FLOW RATE This error message appears when you click the Shutdown button on the Chromeleon Control panel. The message also appears anytime you turn off the pump flow while the suppressor is on. The suppressor is automatically turned off to prevent damage to the suppressor.
  • Page 99 4 • Troubleshooting • Damaged pump piston seal 1. Replace the piston seal (see Section 5.6). 2. If the leak persists, replace the piston (see Section 5.7). • Pump head not tight against casting Carefully tighten the pump head mounting nuts just until the leak stops. DO NOT OVERTIGHTEN! •...

  • Page 100: Pump Difficult To Prime Or Loses Prime

    ICS-2000 Ion Chromatography System • Leaking cell 1. Check the waste lines for blockage; trapped particles can plug the lines and cause a restriction and/or leak. If necessary, clear the waste lines by reversing the direction of flow. 2. Make sure the plumbing downstream from the cell is clear; a blockage may overpressurize the cell, causing it to leak.
  • Page 101 4 • Troubleshooting 2. Select the Options tab (see Figure 4-1). Figure 4-1. ICS-2000 Server Configuration Properties: Options 3. Under Installed Options, verify that the Degas check box is selected. 4. Check the Degas Mode settings. Select either the Monitor option or the Cycle option.

  • Page 102: Pump Does Not Start

    ICS-2000 Ion Chromatography System NOTE It is especially important to regularly replace end-line filters when using aqueous eluents, which may contaminate the filter with bacteria or algae. The bacterial buildup may not be visible. • Blockages in inlet tubing Kinked or clogged tubing causes the pump to be “starved” for eluent. Replace the tubing and fittings (see Section 5.3).

  • Page 103: No Flow

    4 • Troubleshooting • Flow rate is set to 0 Set the flow rate from either the Chromeleon Control panel or the ICS-2000 touch screen page. HOME No Flow • Pump waste or priming valve open (see Figure 4-2). Close the valves by turning the knobs clockwise until fingertight. DO NOT OVERTIGHTEN! Overtightening may damage the valve and the pump head.

  • Page 104: Erratic Flow/Pressure Reading

    ICS-2000 Ion Chromatography System • Eluent generator degas tubing assembly ruptured Replace the EluGen cartridge holder (see Section 5.17). Erratic Flow/Pressure Reading • Pump needs priming Prime the pump (see Section B.13). • Damaged piston seal Replace the piston seal (see Section 5.6).

  • Page 105: Peak "Ghosting

    4 • Troubleshooting • Clogged column bed supports Refer to the instructions in the column manual for troubleshooting guidance. Column manuals are included on the Dionex Reference Library CD-ROM (P/N 053891). • Contaminated columns Clean the columns as instructed in the column manual. •...

  • Page 106: Nonreproducible Peak Height Or Retention Time

    ICS-2000 Ion Chromatography System Nonreproducible Peak Height or Retention Time • Column overloading Dilute the sample (see Section 3.9). • Liquid leaks Locate and eliminate the leaks (see Section 4.2). • Incomplete or imprecise filling of the sample loop 1. Fill the sample loop until excess sample exits the waste line. 2.

  • Page 107: High Cell Output

    4 • Troubleshooting • No flow from pump This condition has several possible causes. See Section 4.4 Section 4.5 for details. • Cell electronics malfunctioning Use the Chromeleon Wellness Panel diagnostics to test the electronics (see Section 5.1 and the Chromeleon online Help for instructions). If the variance reading is outside the tolerance range (less than 1 µS), the electronics are malfunctioning.

  • Page 108: Baseline Noise Or Drift

    ICS-2000 Ion Chromatography System 4.13 Baseline Noise or Drift • Flow system leak; erratic baseline Check all fittings and liquid lines for leaks. Tighten or, if necessary, replace all liquid line connections. Refer to Installation of Dionex Liquid Line Fittings (Document No.

  • Page 109: Vacuum Degas Assembly Does Not Run

    4 • Troubleshooting • Cell above or below temperature Contact Dionex for assistance. • Damaged piston seal Replace the piston seal (see Section 5.6). 4.14 Vacuum Degas Assembly Does Not Run • Degas option not enabled in Chromeleon 1. Open the Chromeleon Server Configuration program. Select the ICS- 2000 device icon under the timebase and select Edit>Properties (or double-click the ICS-2000 device icon).
  • Page 110 ICS-2000 Ion Chromatography System 3. Under Degas Mode, verify that the Always Off option is not selected. If it is, select one of the following settings: • Cycle, and select On: 30 seconds and Off: 10 minutes • Monitor 4. If the Degas Mode settings are correct, but the degas assembly still does not run, test the assembly by selecting the Always On option.

  • Page 111: Service

    5 • Service This chapter describes ICS-2000 service and repair procedures that the user can perform. All procedures not included here, including electronics-related repair procedures, must be performed by Dionex personnel. For assistance, contact Dionex Technical Support. In the U.S., call 1-800-346-6390. Outside the U.S., call the nearest Dionex office.

  • Page 112: Chromeleon Wellness Panel Overview

    ICS-2000 Ion Chromatography System 5.1.1 Chromeleon Wellness Panel Overview NOTE Do not open a System Wellness Control panel if the name includes “Service.pan.” These Wellness panels are reserved for use by Dionex Service Representatives. To Open the Wellness Panel 1. Open the Chromeleon Browser and expand the Dionex Templates folder.
  • Page 113 5 • Service Use the Wellness Panel to perform the following functions: • Calibrate the pump flow rate • Calibrate the vacuum degas assembly • Calibrate the conductivity cell • Test the conductivity cell electronics, using a dummy cell • Upload calibration values from the ICS-2000 to the Chromeleon Wellness database After completing a calibration procedure from the touch screen,...

  • Page 114: Diagnostic And Calibration Touch Screen Overview

    ICS-2000 Ion Chromatography System 5.1.2 Diagnostic and Calibration Touch Screen Overview Select from the touch screen menu of pages. The DIAGNOSTIC page opens (see Figure 5-2). From there, you can access DIAGNOSTIC individual diagnostic and calibration pages. NOTE The Service button provides access to service functions that are performed only by Dionex personnel.

  • Page 115: Calibrating The Conductivity Cell

    5 • Service 5.1.3 Calibrating the Conductivity Cell When to Calibrate • After every 6 months of use NOTE Do not use this procedure to calibrate a new cell. If you are replacing a cell, see the instructions in Section 5.9 install the new cell and calibrate it.
  • Page 116 ICS-2000 Ion Chromatography System 2. On the Wellness Panel under Electric Conductivity Cell Calibration, click Offset Cal, or touch Run Offset Cal on the ICS- 2000 touch screen. 3. When the offset calibration is complete, click Slope Cal on the Wellness Panel, or touch on the ICS-2000 touch Run Slope Cal...

  • Page 117: Calibrating The Flow Rate

    5 • Service 10. Select to begin pumping 1.00 mM KCl through the cell 1.00 mL/min at 1.0 mL/min. 11. Wait until the total conductivity reading stabilizes (in about 15 minutes) and then select Calibrate on the Wellness Panel or the touch screen page.
  • Page 118 ICS-2000 Ion Chromatography System To Calibrate 1. Open the ICS-2000 Wellness Panel in Chromeleon (see Section 5.1.1), or open the ICS-2000 touch screen page FLOW RATE (see Figure 5-5). Figure 5-5. Flow Rate Page 2. Verify that there is 14 ± 1.4 MPa (2000 ± 200 psi) of backpressure. 3.

  • Page 119: Calibrating The Vacuum Degas Assembly

    5 • Service 10. To recheck the value, wait at least 15 minutes, and then repeat the calibration. 11. If you are using the Wellness panel, click to record the new calibration value in the Audit Trail. 12. If you performed the calibration from the ICS-2000 touch screen, record the new calibration values in the Chromeleon Wellness database.

  • Page 120: Isolating A Restriction In The Liquid Lines

    ICS-2000 Ion Chromatography System vacuum degas pressure reading is recorded. When the vacuum degas assembly is running in monitor mode, this calibration value is used to determine when to turn on the degas pump. 3. If you are using the Wellness panel, click to record the new calibration value in the Audit Trail.
  • Page 121 5 • Service To the pump Degas In EGC Vent EGC II KOH, LiOH, MSA, or NaOH Deionized Water NOTE: The EGC Degas fittings cannot be serviced by the user. Inlet Outlet Degas CR-TC From the pulse damper Degas In To the Column inject valve...
  • Page 122 ICS-2000 Ion Chromatography System To the pump Degas In EGC Vent NOTE: The EGC II EGC degas fittings Deionized cannot be Water serviced by the user Inlet Outlet Eluent Out Regen Out Eluent In Degas Regen In Degas In Column Heater Degas Out Conductivity...
  • Page 123 5 • Service To the pump Degas In EGC Vent NOTE: The EGC II EGC degas fittings cannot Deionized be serviced by Water the user Inlet Outlet Degas Degas In Column Heater Degas Out Conductivity Cell Suppressor Cell In Cell Out Pulse Damper Valve Press.

  • Page 124: Replacing Tubing And Fittings

    ICS-2000 Ion Chromatography System Replacing Tubing and Fittings The ICS-2000 is plumbed with the tubing and tubing assemblies listed below. Tubing Size and Type Color Used For 0.125-mm (0.005-in) ID PEEK Connection from pump pulse damper (P/N 044221) to pressure transducer 0.25-mm (0.010-in) ID PEEK Black Connections between other system...

  • Page 125: Rebuilding The Injection Valve

    5 • Service Rebuilding the Injection Valve Dionex recommends rebuilding the injection valve annually. The Injection Valve Rebuild Kit (P/N 057896) contains all required replacement parts. NOTE Substitution of non-Dionex parts may impair valve performance and void the product warranty. 1.

  • Page 126: Cleaning And Replacing The Pump Check Valves

    ICS-2000 Ion Chromatography System Cleaning and Replacing the Pump Check Valves A dirty check valve causes erratic flow rates and pressures. In addition, it may cause the pump to lose prime and/or be difficult to reprime. If possible, replace dirty check valves. If new check valves are not available, follow the instructions for cleaning.
  • Page 127 5 • Service 6. If you are installing new cartridges (P/N 045994) in the existing check valve Single-hole Double-hole housings, place the cartridge in the inlet check valve housing with the double-hole end of the cartridge visible and place the cartridge in the outlet housing with the single-hole end visible.

  • Page 128: Replacing A Pump Piston Seal And Piston Rinse Seal

    ICS-2000 Ion Chromatography System Replacing a Pump Piston Seal and Piston Rinse Seal A damaged seal allows leakage past the piston, as well as leakage from the piston seal wash housing. The pump may be difficult to prime, flow rates may be unstable, and there may be baseline noise.
  • Page 129 5 • Service 5. Place the head (front end down) on a clean work surface and lift off the spacer to expose the piston seal (see Figure 5-12 Figure 5-13). Priming Valve Knob (P/N 055709) Outlet Check Valve Assembly (P/N 045721) O-Ring (P/N 055752) Piston Seal...
  • Page 130 ICS-2000 Ion Chromatography System 6. The piston does not come off as part of the pump head assembly because it is captured by a magnetic retention system. After removing the pump head, hold the shaft of the piston (near the base), tilt the piston slightly, and pull the piston away from the pump.
  • Page 131 5 • Service Removing the Piston Seal from the Head 1. Fill the head cavity with deionized water by injecting the liquid through either the piston opening or the inlet check valve. 2. Reinsert the piston approximately 3 mm (0.125) inch into the seal. 3.

  • Page 132: Replacing A Pump Piston

    ICS-2000 Ion Chromatography System Reinstalling the Head and Piston Dionex recommends reinstalling the head and piston as a single assembly, so that the piston centers itself onto the magnetic follower. 1. Hold the assembled spacer and guide with the drain tubes aligned vertically and press the spacer into the head until it is flush with the indented surface of the head.

  • Page 133: Replacing The Waste Valve Or Priming Valve O-Ring

    5 • Service Replacing the Waste Valve or Priming Valve O-Ring A damaged O-ring causes leakage around the base of the waste valve or priming valve knob. 1. Close the eluent valve from the Control panel in Chromeleon or select Eluent on the touch screen page.
  • Page 134 ICS-2000 Ion Chromatography System 4. If the O-ring is removed with the valve knob in Step 3, pull the O-ring off the end of the knob (see Figure 5-15). If the O-ring is not removed with the valve knob, insert a thin object (for example, the bent end of a paper clip) into the cavity in the pump head and carefully pull out the O-ring.

  • Page 135: Replacing The Conductivity Cell

    5 • Service Replacing the Conductivity Cell 1. Turn off the ICS-2000 power. 2. Open the front door and disconnect the tubing from the CELL IN CELL fittings (see Figure 5-16, View A). Loosen the two screws on the cell front cover.
  • Page 136 ICS-2000 Ion Chromatography System 5. Slide the new cell assembly into the opening in the component panel until it stops and the cell cover is flush against the component panel. The cell’s electronic connector automatically plugs into a connector inside the ICS-2000 when you push the assembly into the opening.
  • Page 137 5 • Service To calibrate the cell on the ICS-2000 touch screen page: DIAGNOSTIC a. Go to the page and touch Electric Conductivity Cell DIAGNOSTIC b. On the page (see Figure 5-18), ELECTRIC CONDUCTIVITY CALIBRATION touch Run Offset Cal Figure 5-18. Electric Conductivity Calibration Page c.

  • Page 138: Replacing The Suppressor

    ICS-2000 Ion Chromatography System 5.10 Replacing the Suppressor Refer to the suppressor manual for guidance about when to replace a suppressor. Suppressor manuals are included on the Dionex Reference Library CD-ROM (P/N 053891). 1. Refer to the suppressor quick start guide (shipped with the suppressor) for instructions on preparing the suppressor before initial use and for additional installation details.

  • Page 139: Replacing The Column Heater

    5 • Service 10. Push the cables into the opening in the component panel. 11. Align the top of the suppressor with the guide line printed on the component panel (see Figure 5-20) and align the slots on the rear of the suppressor Suppressor with the tabs on the panel.
  • Page 140 ICS-2000 Ion Chromatography System c. Unscrew the six thumbscrews on the top metal plate (see Figure 5-21) and remove the plate. Thumbscrews (6) Figure 5-21. ICS-2000 Column Heater Cover Removed d. Remove the columns from the column heater. e. Pull the column heater straight toward you to remove it from the component panel.
  • Page 141 5 • Service 9. Enter the column heater calibration values in Chromeleon or on the ICS-2000 touch screen page as follows. DIAGNOSTIC To enter the calibration values in Chromeleon: a. Open the Wellness Panel (see Section 5.1.1) and click Calibration Details.

  • Page 142: Replacing The Column Heater Heat Exchanger

    ICS-2000 Ion Chromatography System c. To ensure the calibration values are recorded in the ICS-2000 memory, do not turn off the ICS-2000 power for at least 1 minute after entering the values. d. Record the calibration values in the Chromeleon Wellness database. To do this, open the Wellness Panel (see Section 5.1.1) and click Upload under...

  • Page 143: Replacing The Eluent Valve

    5 • Service tubing) and press the heat exchanger onto the heater. Replace the screws removed in Step 10. Connect the new heat exchanger inlet line to Port C on the injection valve and connect the outlet line to the guard column inlet. 11.
  • Page 144 ICS-2000 Ion Chromatography System 6. Pull the valve straight out from the component panel and begin pulling the attached cable out of the opening in the panel. 7. The cable is connected to a matching cable inside the ICS- 2000. Continue pulling until Eluent Valve the connectors for the two Cable...

  • Page 145: Replacing The Leak Sensor

    5 • Service 5.14 Replacing the Leak Sensor 1. Turn off the power to the ICS- 2000. 2. Open the ICS-2000 front door. 3. Loosen the screw on the front of the leak sensor (see Figure 5-27). Note: The screw remains attached to the sensor.

  • Page 146: Priming The Pump With Isopropyl Alcohol

    ICS-2000 Ion Chromatography System 5.15 Priming the Pump with Isopropyl Alcohol NOTE Prime the pump heads with isopropyl alcohol only if the standard priming procedures described Section B.13 are unsuccessful. 1. Connect a 10-mL syringe (P/N 054578) filled with Secondary Pump Head isopropyl alcohol (IPA) to the port in the primary...

  • Page 147: Changing Main Power Fuses

    5 • Service Isopropyl alcohol may damage some columns. Be sure to thoroughly rinse the alcohol from the pump, using the process described in Step 8. Close the waste valve. Do not overtighten. 9. Select the flow rate required for the analysis. 10.

  • Page 148: Replacing An Elugen Cartridge

    ICS-2000 Ion Chromatography System 3. Replace the two fuses in the holder with new IEC 127 fast-blow fuses rated 3.15 amps (P/N 954745). Dionex recommends always replacing both fuses. 4. Reinsert the fuse holder into its compartment and push in until the tab clicks in place.

  • Page 149: Replacing A Koh, Lioh, Msa, Or Naoh Elugen

    5 • Service 5.17.1 Replacing a KOH, LiOH, MSA, or NaOH EluGen Cartridge 1. Turn off the pump from the ICS-2000 Control panel in Chromeleon or from the ICS-2000 touch screen page. HOME 2. Disconnect the EluGen cartridge cable from the connector on EGC-1 the ICS-2000 top cover.
  • Page 150 ICS-2000 Ion Chromatography System 7. Turn the cartridge upside down and set it in the EluGen cartridge service area on top of the ICS-2000 (see Figure 5-32 Figure 5-33). EluGen Cartridge Service Area Figure 5-32. EluGen Cartridge Service Area Figure 5-33. EluGen Cartridge in the Service Area 5-40 Doc.

  • Page 151: Cartridge

    5 • Service 8. Disconnect the EGC IN lines EGC OUT EGC OUT OUTLET from the EGC IN INLET Port ports on the OUTLET INLET EluGen cartridge (see Port Figure 5-34). Figure 5-34. Disconnect the EluGen Cartridge Inlet and Outlet Port Lines 9.
  • Page 152 ICS-2000 Ion Chromatography System 12. Set the EluGen cartridge in the service area on top of the ICS-2000 and orient it with the cable toward the EGC holder (see Figure 5-36). EluGen Cartridge Cable Figure 5-36. EluGen Cartridge in Service Area 13.
  • Page 153 5 • Service NOTE The backpressure coil connection is temporary; it is used only during the EluGen cartridge conditioning procedure described in “Conditioning the EluGen Cartridge” on page 5-46. 16. Turn the EluGen cartridge over, with the fittings facing down. Shake the EluGen cartridge vigorously and tap it with the palm of your hand 10 to 15 times.
  • Page 154 ICS-2000 Ion Chromatography System 18. Orient the cartridge’s blue 4-pin electrical cable connector as shown in Figure 5-39. Push it onto the EGC-1 connector on the ICS- 2000. Twist the ring on the cable connector fingertight to secure it. Figure 5-39. Connecting the EluGen Cartridge Cable 19.
  • Page 155 5 • Service Recording the EluGen Cartridge Serial Number in Chromeleon The serial number is printed on the EluGen cartridge label. 1. Open the Chromeleon Server Configuration program (select Start > Programs (or All Programs) > Chromeleon > Server Configuration). 2.
  • Page 156 ICS-2000 Ion Chromatography System Conditioning the EluGen Cartridge Always condition the EluGen cartridge before initial use. This requires directing the backpressure coil connected to the EluGen cartridge outlet to waste, and then generating 50 mM of eluent at 1.00 mL/min for 30 minutes.
  • Page 157 5 • Service 11. Lift the EluGen cartridge out of the EGC OUT holder, turn it over, EGC IN OUTLET and set it in the Port INLET service area. Port 12. Disconnect the backpressure coil from the EluGen cartridge OUTLET port 13.
  • Page 158 ICS-2000 Ion Chromatography System 5.17.2 Replacing a K Cartridge 1. Turn off the pump from the ICS-2000 Control panel in Chromeleon or from the ICS-2000 touch screen page. HOME 2. Disconnect the EluGen cartridge cable from the connector on EGC-1 the ICS-2000 top cover.
  • Page 159 5 • Service 7. Turn the cartridge upside down and set it in the EluGen cartridge service area on top of the ICS-2000 (see Figure 5-32 Figure 5-33). EluGen Cartridge Service Area Figure 5-43. EluGen Cartridge Service Area Figure 5-44. EluGen Cartridge In the Service Area 5-49 Doc.
  • Page 160 ICS-2000 Ion Chromatography System 8. Disconnect the EGC IN lines EGC OUT EGC OUT OUTLET from the INLET EGC IN Port fittings on the OUTLET INLET EluGen cartridge (see Port Figure 5-34). Figure 5-45. Disconnect the EluGen Cartridge Inlet and Outlet Port Lines 9.
  • Page 161 5 • Service 12. Set the EluGen cartridge in the service area on top of the ICS-2000 and orient it with the cable toward the EGC holder (see Figure 5-36). EluGen Cartridge Cable Figure 5-47. EluGen Cartridge in Service Area 13.
  • Page 162 ICS-2000 Ion Chromatography System 16. Slide the EluGen cartridge down into the holder until it stops (see Figure 5-48). Make sure the cable remains out of the holder. NOTE To avoid crimping the lines, do not twist the cartridge when you slide it into the holder.
  • Page 163 5 • Service Recording the EluGen Cartridge Serial Number in Chromeleon The serial number is printed on a label on the EluGen cartridge. 1. Open the Server Configuration program (select Start>Programs (or All Programs)>Chromeleon>Server Configuration). 2. Double-click the ICS-2000 system icon. The properties dialog box for the ICS-2000 appears.
  • Page 164 ICS-2000 Ion Chromatography System Plumbing the EluGen Cartridge for Conditioning with Carbonate Eluent Figure 5-50 illustrates the liquid flow path during the EluGen cartridge conditioning procedure with carbonate eluent. EGC VENT EGC II K CO Send to waste Outlet Inlet TO DEGAS ELUENT IN DEGAS...
  • Page 165 5 • Service 1. Disconnect the line from the EGC TO INJ VALVE IN - P/ELUENT OUT Mixer inlet. 2. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) and a 10-32 to 10-32 coupler (P/N 042627) in the ICS- 2000 Ship Kit (P/N 064375).
  • Page 166 ICS-2000 Ion Chromatography System Conditioning the EluGen Cartridge Always condition the EluGen cartridge for 30 minutes before initial use. 1. Make sure the eluent reservoir is filled with ASTM filtered, Type I (18-megohm) deionized water. 2. Verify that the suppressor is off. To avoid damaging the suppressor, always turn off the suppressor before conditioning the EluGen cartridge.
  • Page 167 5 • Service Plumbing the EluGen Cartridge and EPM for Conditioning with Carbonate/Bicarbonate Eluent Figure 5-52 illustrates the liquid flow path through the eluent generator during conditioning with carbonate/bicarbonate eluent. EGC VENT EGC II K CO Send to waste Inlet Outlet (red label)
  • Page 168 ICS-2000 Ion Chromatography System 1. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) and 10-32 to 1/4-28 coupler (P/N 042806) in the ICS- 2000 Ship Kit (P/N 064375). 2. Disconnect the line from the EGC TO INJ VALVE IN - P/ELUENT OUT Mixer inlet.
  • Page 169 5 • Service Conditioning the EluGen Cartridge Always condition the EluGen cartridge for 30 minutes before initial use. 1. Make sure the eluent reservoir is filled with ASTM filtered, Type I (18-megohm) deionized water. 2. Verify that the suppressor is off. To avoid damaging the suppressor, always turn off the suppressor before conditioning the EluGen cartridge.

  • Page 170: Replacing The Cr-Tc

    ICS-2000 Ion Chromatography System 5.18 Replacing the CR-TC 1. Turn off the pump from the ICS-2000 Control panel in Chromeleon or from the touch screen page. HOME 2. Follow the instructions in Section 5.17.1, Step 2 through Step 8 to disconnect the EluGen cartridge and CR-TC cables, cap the EluGen cartridge vent opening, remove the EluGen cartridge from the holder, and disconnect the inlet and outlet tubing.
  • Page 171 5 • Service 10. Check that none of the lines are caught under the CR-TC and then push the CR-TC onto the metal stud inside the EGC holder. Push down until the CR-TC snaps into place (see Figure 5-55). Figure 5-55. CR-TC Installed in Holder 11.
  • Page 172 ICS-2000 Ion Chromatography System 15. Reinstall the EluGen cartridge in the holder. 16. Remove the cap on the luer adapter by twisting. Save the cap. 17. Twist the luer lock and line into the luer adapter. EGC VENT 18. Do not connect the EluGen cartridge cable until after hydrating the CR-TC.
  • Page 173 5 • Service 1. Locate the 10-32 to 1/4-28 coupler (P/N 042806) in the ICS-2000 Ship Kit (P/N 064375). 2. Use the coupler to connect the line labeled TO INJ VALVE IN - and the line labeled P/ELUENT OUT SRS/AES REGEN OUT 3.

  • Page 174: Replacing The Epm Electrolytic Ph Modifier

    ICS-2000 Ion Chromatography System 5.19 Replacing the EPM Electrolytic pH Modifier 1. Turn off the pump from the ICS-2000 Control panel in Chromeleon or from the touch screen page. HOME 2. Disconnect the EPM electrical cable from the connector on the ICS- EGC-2 2000 top cover.

  • Page 175: Recording The Epm Serial Number In Chromeleon

    5 • Service 5.19.1 Recording the EPM Serial Number in Chromeleon The serial number is printed on a label on the EPM. 1. Open the Server Configuration program (select Start > Programs (or All Programs) > Chromeleon > Server Configuration). 2.

  • Page 176: Plumbing The Epm For Hydrating And Conditioning

    ICS-2000 Ion Chromatography System 5.19.2 Plumbing the EPM for Hydrating and Conditioning Figure 5-52 illustrates the liquid flow path during hydration and conditioning. EGC VENT EGC II K CO Send to waste Inlet Outlet (red label) TO CR-TC/EPM (yellow label) ELUENT OUT (yellow label) TO DEGAS...
  • Page 177 5 • Service 1. Disconnect the line from the EGC TO INJ VALVE IN - P/ELUENT OUT Mixer inlet. 2. Use a 10-32 to 10-32 coupler (P/N 042627) to connect the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) to the line.

  • Page 178: Hydrating And Conditioning The Epm

    ICS-2000 Ion Chromatography System 5.19.3 Hydrating and Conditioning the EPM Follow the steps below to hydrate the EPM for 5 minutes and then condition the EPM for 30 minutes. 1. Fill the eluent reservoir with ASTM filtered, Type I (18-megohm) deionized water.
  • Page 179 5 • Service 15. Reconnect the line to the suppressor TO SRS/AES REGEN OUT REGEN port. 5.20 Replacing the EGC CO Mixer 5.20.1 Installing the New EGC CO Mixer 1. Turn off the pump from the ICS-2000 Control panel in Chromeleon or from the touch screen page.
  • Page 180 ICS-2000 Ion Chromatography System 4. Connect the line to the mixer inlet and connect TO EGC CO3 MIXER IN line to the mixer outlet. TO EGC CO3 MIXER OUT TO EGC CO3 MIXER OUT TO EGC CO3 MIXER IN Figure 5-62. ICS-2000 with an EGC CO Mixer Installed 5.20.2 Filling the EGC CO Mixer with Deionized Water...
  • Page 181 5 • Service TO PUMP/ DAMPER (disconnect this line) Pump Outlet (green tubing) Figure 5-63. Disconnect the Cartridge Inlet and Pump Outlet 3. Disconnect the line from the TO EGC CO3 MIXER IN TO INJ VALVE IN - line. P/ELUENT OUT 5-71 Doc.
  • Page 182 ICS-2000 Ion Chromatography System 4. Connect the pump outlet to the mixer inlet (see Figure 5-64). Pump Outlet Mixer Inlet Figure 5-64. Pump Outlet Connected to Mixer Inlet for Mixer Hydration 5. Disconnect the mixer outlet line (labeled ) from TO INJ VALVE PORT P port ) on the injection valve.

  • Page 183: Filling The Egc Co3 Mixer With Eluent

    5 • Service It takes about 5 minutes to fill a 4-mm mixer and about 2 minutes to fill a 2-mm mixer. 12. Turn off the pump. 5.20.3 Filling the EGC CO Mixer with Eluent Before initial use, follow the steps in this section to fill the EGC CO Mixer with the eluent required for the application to be run.
  • Page 184 ICS-2000 Ion Chromatography System Filling the EGC CO Mixer with Carbonate Eluent Figure 5-65 illustrates the liquid flow path through the eluent generator components during initial filling of the EGC CO Mixer with carbonate eluent. To the EluGen From the EluGen cartridge inlet port cartridge outlet port TO DEGAS...
  • Page 185 5 • Service 1. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) and a 10-32 to 10-32 coupler (P/N 042627) in the ICS- 2000 Ship Kit (P/N 064375). 2. Use the coupler to connect one end of the backpressure coil to the line.
  • Page 186 ICS-2000 Ion Chromatography System 12. Run at these settings until a consistent flow of eluent is exiting the mixer outlet. At a flow rate of 1 mL/min it will take about 16 minutes to fill a 4-mm mixer and about 5 minutes to fill a 2-mm mixer. NOTE The void volume of the 4-mm mixer is about 16 mL.
  • Page 187 5 • Service Filling the EGC CO Mixer with Carbonate/Bicarbonate Eluent Figure 5-66 illustrates the liquid flow path through the eluent generator components during initial filling of the EGC CO Mixer with carbonate/bicarbonate eluent. To the EluGen From the EluGen cartridge inlet port cartridge outlet port (red)
  • Page 188 ICS-2000 Ion Chromatography System 1. Locate the following items in the ICS-2000 Ship Kit (P/N 064375): a yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765), a 10-32 to 10-32 coupler (P/N 042627), and a 10-32 to 1/4-28 coupler (P/N 042806). 2.
  • Page 189 5 • Service 5. Verify that the suppressor is off. To avoid damaging the suppressor always turn off the suppressor before filling the mixer. The pump flow is on during filling, however, no flow reaches the suppressor. 6. Verify that the Automatically turn on with pump option is not selected on the ICS-2000 touch screen...

  • Page 190: Replacing The Egc Holder And Degas Assembly

    ICS-2000 Ion Chromatography System 5.21 Replacing the EGC Holder and Degas Assembly 5.21.1 Disconnecting and Removing the EluGen Cartridge 1. Turn off the power to the ICS-2000. 2. Disconnect the following EGC holder liquid lines: • Disconnect the line labeled TO INJ VALVE IN - P/ELUENT OUT from either port ) on the injection valve, or if an EGC CO...
  • Page 191 5 • Service 4. Remove the Luer Adapter line (see VENT Luer Lock Figure 5-68) from the EluGen cartridge by unscrewing the EGC Vent luer lock from the Line luer adapter. If necessary, use a wrench to hold the luer adapter in place while unscrewing the vent line.
  • Page 192 ICS-2000 Ion Chromatography System 9. Disconnect the lines from the EGC IN EGC OUT INLET ports on the cartridge. OUTLET 10. Lift the EGC holder out of the top cover and pull the liquid lines through the tubing chase (see Figure 5-70) to remove them from the ICS-...

  • Page 193: Removing The Cr-Tc And Reinstalling It In The New Egc

    5 • Service 5.21.2 Removing the CR-TC and Reinstalling it in the New EGC Holder 1. Turn the holder upside down to view the CR-TC. 2. Grasp the top of the CR- Disconnect liquid TC and lift up to lines from 4 ports disconnect it from the holder.
  • Page 194 ICS-2000 Ion Chromatography System 5. Check that none of the lines are caught under the CR-TC and then push the CR-TC onto the metal stud inside the EGC holder. Push down until the CR-TC snaps in place (see Figure 5-73). Figure 5-73.

  • Page 195: Removing The Epm And Reinstalling It In The New Egc

    5 • Service 9. Feed the following liquid lines from the bottom of the holder through the tubing chase to the ICS-2000 front component panel. • TO SRS/AES REGEN OUT • TO INJ VALVE IN - P/ELUENT OUT • TO PUMP/DAMPER 10.
  • Page 196 ICS-2000 Ion Chromatography System 4. Thread the three lines from the EPM (red, yellow, and blue labels) through the opening on the side of the new holder (see Figure 5-75). TO CR-TC/EPM TO CR-TC/EPM ELUENT IN ELUENT OUT (red label) (yellow label) TO CR-TC/EPM REGEN OUT...

  • Page 197: Installing The New Egc Holder Without A Cr-Tc Or Epm

    5 • Service 8. Turn the EGC holder right side up and set it in place on top of the ICS-2000. 9. Slide the EPM mounting bracket and connected EPM onto the side of the EGC holder. 10. Feed the following liquid lines through the tubing chase to the ICS- 2000 front component panel.

  • Page 198: Reinstalling The Elugen Cartridge

    ICS-2000 Ion Chromatography System • Connect the line labeled to the TO INJ VALVE IN - P/ELUENT OUT EGC CO Mixer inlet. • Connect the line labeled to the pulse damper. TO PUMP/DAMPER Direct the line from the top of the ICS- WASTE, GAS SEPARATOR 2000 to the rear panel.

  • Page 199: A • Specifications

    A • Specifications A.1 Electrical Main Power 85 to 265 Vac, 47 to 63 Hz. The ICS-2000 power supply is auto- sensing and requires no voltage adjustment Fuses Two 3.15 amp fast-blow IEC 127 fuses (P/N 954745) A.2 Physical Dimensions Height: 56.1 cm (22.1 in) (without Width: 22.4 cm (8.8 in)

  • Page 200: Front Panel

    ICS-2000 Ion Chromatography System A.4 Front Panel Power LED On when power is present; off when no power is present LCD Touch ¼ VGA (320 x 240 pixels) liquid crystal display with touch screen, Screen which provides: • Local control of ICS-2000 components: pump, detector, suppressor, injection valve, eluent generator, CR-TC, column heater, vacuum degas, TTLs, and relays •...

  • Page 201: Detector

    A • Specifications A.6 Detector 0 to 15000 µS (digital output) Range Temperature 1.7% per °C Compensation Cell Drive 8 kH square wave Linearity Correlation >0.999 %RSD <5% to 800 µS A.7 Conductivity Cell with Heat Exchanger Cell Body PEEK Electrodes Passivated 316 stainless steel 1.0 µL...

  • Page 202: Column Heater

    ICS-2000 Ion Chromatography System A.10 Column Heater Operating 30 to 60 °C (86 to 140 °F); must be at least 5 °C above the ambient Temperature temperature A.11 Eluent Generator Concentration Dependent on the EluGen cartridge type, the cartridge configuration Range (single or linked dual), and the flow rate.
  • Page 203 A • Specifications EluGen Eluent Concentration Range Comment Cartridges /EPM 0.1 to 15 mM at 0.1 to 1.0 mL/min flow The total of the eluent 0.1 to X mM at 1.0 to 2.0 mL/min flow concentrations from the Electrolytic pH X = 15/flow EluGen cartridge and the Modifier...
  • Page 204 ICS-2000 Ion Chromatography System Doc. 031857-03 4/06...

  • Page 205: B • Installation

    B • Installation This chapter provides instructions for the initial installation of the ICS-2000 Ion Chromatography System. NOTE These installation instructions are also shipped with the ICS-2000 as a stand-alone printed manual (Document No. 031858). NOTE The order of the steps is important. It is especially important to install Chromeleon before connecting the USB cable and turning on the ICS-2000 power.

  • Page 206: Unpacking The Ics-2000 System

    ICS-2000 Ion Chromatography System B.2 Unpacking the ICS-2000 System 1. After removing the ICS-2000 from the shipping box and removing the top layer of foam, remove the next layer of foam (see Figure B-1). EGC Holder (upside down) Eluent Bottle Figure B-1.
  • Page 207 Unpacking the ICS-2000 System 4. Remove the next layer of foam and remove the Ship Kit box (see Figure B-3). Ship Cutouts for lifting Figure B-3. ICS-2000 with Ship Kit 5. The bottom foam layer has two cutouts on either side of the ICS-2000 module;...

  • Page 208: Unpacking The Computer (North America Only

    ICS-2000 Ion Chromatography System B.2.1 Unpacking the Computer (North America only) NOTE These instructions assume that Chromeleon software was installed on the computer before shipping. 1. Remove the central processing unit (CPU), keyboard, mouse, and all documentation from the computer box and place these items on a workbench.
  • Page 209 Installing Chromeleon B.3 Installing Chromeleon NOTES • Windows 2000 or Windows XP is required for controlling the ICS-2000 ® with Chromeleon. Chromeleon version 6.5 SP2 or later is required. • To install Chromeleon, you must log onto Windows 2000 or XP with administrator privileges.
  • Page 210 ICS-2000 Ion Chromatography System IMPORTANT Installing DX-LAN Devices If you will also be installing a module that requires a DX-LAN interface connection, verify that the following steps are completed before continuing: 1. If it is not already installed, install the DX-LAN card in the module. Refer to the operator’s manual for the module for installation instructions.
  • Page 211 Installing the Chromeleon Software License • Select the Install the software automatically (Recommended) option (see Figure B-4) (it is not necessary to insert a hardware installation CD or floppy disk). Figure B-4. Windows Found New Hardware Wizard 3. If it is not already running, start the Chromeleon Server Monitor by double- clicking the Server Monitor icon on the Windows taskbar.
  • Page 212 ICS-2000 Ion Chromatography System 4. If the server is not already running, start it by clicking Start (see Figure B-5). Figure B-5. Server Not Running 5. Check the server status: • If “Chromeleon Server is running idle” is displayed, the license Key Code was entered before shipping the computer, and you can omit the remaining steps in this section.
  • Page 213 Installing the Chromeleon Software License 7. Select File>Preferences to open the Preferences dialog box (see Figure B-7). Select the License tab and click Dongle. Enter the Key Code exactly as it appears on the Chromeleon Software License key code sheet. Click OK. Figure B-7.

  • Page 214: Connecting The Ics-2000 To The Chromeleon Pc

    ICS-2000 Ion Chromatography System B.5 Connecting the ICS-2000 to the Chromeleon PC The ICS-2000 rear panel provides a USB receptacle for connecting the ICS-2000 to a USB port on the Chromeleon PC. The rear panel also provides two USB ports for connecting other Dionex USB-compliant modules to the system.

  • Page 215: Connecting Additional Usb Devices

    Connecting the ICS-2000 to the Chromeleon PC B.5.2 Connecting Additional USB Devices The ICS-2000 provides two USB ports for connecting additional Dionex USB-compliant modules to the system. To connect an additional USB module, plug the “A” connector on a USB cable into an “A”...

  • Page 216: Connecting The Power Cord

    ICS-2000 Ion Chromatography System B.6 Connecting the Power Cord Connect the power cord (IEC 320 C13) (ordered separately) from the main power receptacle on the rear panel to a grounded power source. The ICS-2000 power supply is auto-sensing, so no adjustment is required to select the line voltage. SHOCK HAZARD—To avoid electrical shock, use a grounded receptacle.

  • Page 217: Turning On The Ics-2000 Power

    Turning On the ICS-2000 Power B.7 Turning On the ICS-2000 Power Before turning on the ICS-2000 power, verify that Chromeleon software is installed. See Section B.3 for installation instructions. If Chromeleon is not installed first, Windows will be unable to identify the new USB device.
  • Page 218 ICS-2000 Ion Chromatography System 5. The Found New Hardware Wizard appears. This wizard installs the ICS-2000 device into the Windows Device Manager. Complete the wizard by selecting the following options: • If asked whether Windows can connect to Windows Update to search for software, select No, not this time.

  • Page 219: Setting Up Chromeleon

    Setting Up Chromeleon B.8 Setting Up Chromeleon NOTE This section provides brief instructions for setting up Chromeleon. For details about any of these steps, refer to the Chromeleon user’s guide or online Help. B.8.1 Assigning the ICS-2000 to a Timebase After the ICS-2000 power is turned on and the Windows Found New Hardware Wizard is finished, the Chromeleon USB Auto Configuration Wizard automatically appears.
  • Page 220 ICS-2000 Ion Chromatography System To create a new timebase: 1. Select the Create a new timebase option and click OK. Page 2 of the wizard appears (see Figure B-14). Figure B-14. Chromeleon USB Auto Configuration Wizard: Page 2 2. Review the names displayed in the Unassigned USB Devices Detected window.
  • Page 221 Setting Up Chromeleon 3. In the Mode window, select a pre-defined configuration template for the new timebase. Typically for the ICS-2000, one configuration is available: Single (1 timebase with single system but no sharing device). The Description box lists the devices included in the mode. Devices in brackets are optional.
  • Page 222 ICS-2000 Ion Chromatography System To verify the configuration: 1. In the Server Configuration program, click the + icon next to the server name to expand the folder. The timebase you created is displayed (see Figure B-15). Figure B-15. Server Configuration with ICS-2000 Timebase 2.
  • Page 223 Setting Up Chromeleon 3. The ICS-2000 System dialog box opens (see Figure B-16). The General page shows the Device Name (Pump_ECD), the Mode (Live), and the Module Serial No. Figure B-16. ICS-2000 System Properties 4. Close the dialog box and then save the configuration (select File>Save Installation).

  • Page 224: Assigning Dx-Lan Devices To The Timebase (Optional

    ICS-2000 Ion Chromatography System B.8.2 Assigning DX-LAN Devices to the Timebase (Optional) Follow the instructions in this section if you are adding one or more devices to the timebase that are connected to the PC through a DX-LAN interface (not through USB connections). 1.
  • Page 225 Setting Up Chromeleon 3. Under Manufacturers, select Dionex: IC Modules and then select the device from the Devices list. Figure B-18. Add Device to Timebase Dialog Box 4. Click OK. A configuration properties dialog box for the device appears. Figure B-19 shows an example of the properties for an AS50 Autosampler.
  • Page 226 ICS-2000 Ion Chromatography System 5. On the General tab page, clear the Demo Mode check box if it is currently selected, and then select the DX-LAN Device ID number. Note: Devices display their DX-LAN ID numbers when their power is turned on. 6.

  • Page 227: Installing And Plumbing The Columns And Suppressor

    Installing and Plumbing the Columns and Suppressor B.9 Installing and Plumbing the Columns and Suppressor NOTE This section provides brief installation and plumbing instructions for the columns and suppressor. Before beginning the installation, refer to the column and suppressor manuals detailed start-up installation instructions.

  • Page 228: Installing The Columns

    ICS-2000 Ion Chromatography System 4. Unscrew the six thumbscrews on the top metal plate (see Figure B-21) and remove the plate. Thumbscrew (6) 5. Go on to Section B.9.2. Figure B-21. ICS-2000 Column Heater Cover Removed B.9.2 Installing the Columns 1.

  • Page 229: Installing The Suppressor

    Installing and Plumbing the Columns and Suppressor B.9.3 Installing the Suppressor 1. Locate the suppressor mounting tabs on the component panel (see Figure B-22). Reach into the opening Suppressor between the tabs and Mounting Tabs pull the yellow Suppressor connector and attached Cable Connector cable slightly out of the Guideline for...
  • Page 230 ICS-2000 Ion Chromatography System 6. Press the suppressor onto the tabs and then slide it down to lock it in place. Pull slightly on the center of the suppressor to verify that it is securely fastened. Figure B-23 shows an SRS suppressor installed on the component panel.

  • Page 231: Connecting The Waste Lines

    Connecting the Waste Lines B.10 Connecting the Waste Lines The ICS-2000 has the following waste lines: • carries prime waste from the pump. PUMP WASTE • carries sample overflow from the injection valve. TO WASTE OUT • carries system waste from the suppressor. WASTE, GAS SEPARATOR 1.
  • Page 232 ICS-2000 Ion Chromatography System that the gas separator waste tube and the waste container are open to the atmosphere. 4. Go on to Section B.11. DO NOT CAP THE WASTE CONTAINER: The ICS-2000, Atlas Electrolytic Suppressor, and Self-Regenerating Suppressor (SRS) use an electrolytic process that results in the production of small amounts of oxygen or hydrogen gas.

  • Page 233: Setting Up The Eluent Reservoir

    Setting Up the Eluent Reservoir B.11 Setting Up the Eluent Reservoir 1. Rinse the eluent reservoir. 2. Fill the reservoir with ASTM filtered, Type I (18-megohm) deionized water. 3. Place the reservoir on top of the ICS-2000. 4. Install the cap and handtighten. NOTE The ICS-2000 does not require pressurized reservoirs.

  • Page 234: Setting The Eluent Level

    ICS-2000 Ion Chromatography System B.12 Setting the Eluent Level After filling the reservoir, open the touch screen page and enter the volume HOME of liquid in the reservoir in the Eluent Level field (see Figure B-25), or enter the volume in the Eluent Bottle field on the Chromeleon Control panel (see Figure B-26).

  • Page 235: Priming The Pump

    Priming the Pump B.13 Priming the Pump 1. Verify that the eluent reservoir is filled, the reservoir cap is installed and handtightened, and the liquid line from the ICS-2000 to the reservoir cap is connected (see Section B.11). 2. Verify that the waste lines are directed to a waste container. 3.

  • Page 236: Priming The Eluent Lines With A Syringe

    ICS-2000 Ion Chromatography System B.13.1 Priming the Eluent Lines with a Syringe NOTE Prime the eluent lines after initial installation, after changing eluents, or when eluent lines are empty. 1. Verify that the pump is turned off. 2. Connect a 10 mL syringe (P/N 054578) to the port in the priming valve on the primary pump head (see Figure B-27).
  • Page 237 Priming the Pump 5. Touch to open the touch screen page (see Figure B-28). PUMP PUMP Under , touch the button. Eluent Valve Open Figure B-28. Pump Page: Eluent Valve Open 6. Draw the syringe back to begin pulling eluent through the flow path. It may take several syringe draws to remove all air or previous eluent from the tubing.

  • Page 238: Priming The Pump Heads With The Prime Button

    ICS-2000 Ion Chromatography System B.13.2 Priming the Pump Heads with the Prime Button NOTE Perform this procedure after priming the eluent lines (see Section B.13.1). 1. Check that the priming valve on the primary pump head is closed (see Figure B-29).

  • Page 239: Setting Up The Eluent Generator

    Setting Up the Eluent Generator B.14 Setting Up the Eluent Generator Before Beginning Remove the EluGen cartridge (EGC) and the other items to be installed from their shipping containers. Depending on the type of eluent to be generated, the other items may include a Continuously Regenerated Trap Column (CR-TC), an EPM Electrolytic pH Modifier, and/or an EGC CO Mixer.

  • Page 240: Eluent Generator Setup For Koh, Lioh, Msa, And Naoh

    ICS-2000 Ion Chromatography System B.14.1 Eluent Generator Setup for KOH, LiOH, MSA, and NaOH Eluents Follow the instructions in this section if you are installing a KOH, LiOH, MSA, or NaOH EluGen cartridge and a CR-TC. • If you are installing a K cartridge, an EPM, and an EGC CO Mixer (for generating carbonate/bicarbonate eluent), go to Section...
  • Page 241 Setting Up the Eluent Generator Items Required to Complete the Setup Procedure In addition to the EluGen cartridge and the CR-TC, you will need the following items to complete the eluent generator setup. Item Use To Yellow 0.5 mL/min, 7 MPa 053765 Provide backpressure during (1000 psi) backpressure coil...
  • Page 242 ICS-2000 Ion Chromatography System Figure B-30 illustrates the ICS-2000 after the EluGen cartridge is installed. EGC OUT EluGen Cartridge (EGC) EGC Holder EGC and CR-TC Cable Connections Figure B-30. ICS-2000 with an EluGen Cartridge Installed (Top View) B-38 Doc. 031857-03 4/06...
  • Page 243 Setting Up the Eluent Generator Figure B-31 illustrates the liquid flow path through the eluent generator components after all setup steps are complete and the eluent generator is ready for operation. EGC VENT EGC II KOH, LiOH, MSA, or NaOH Send to waste Inlet Outlet...
  • Page 244 ICS-2000 Ion Chromatography System Installing the CR-TC 1. Tilt the cartridge holder to view the bottom of the holder and brace the holder against the back corner of the ICS-2000 (see Figure B-32). Metal ball stud for mounting the CR-TC Figure B-32.
  • Page 245 Setting Up the Eluent Generator 4. With the CR-TC oriented as shown in Figure B-34, connect each line to the corresponding port on the CR-TC. NOTE To help retain the fittings and ferrules on the lines, remove the coupler from each line just before connecting the line to a port.
  • Page 246 ICS-2000 Ion Chromatography System 6. Feed the CR-TC cable through the slot on the side of the EGC holder (see Figure B-36). CR-TC Cable Figure B-36. Installing the CR-TC 7. Turn the EGC holder right-side up and set it back into position. Installing the EluGen Cartridge 1.
  • Page 247 Setting Up the Eluent Generator 2. Set the EluGen cartridge in the service area on top of the ICS-2000 and orient it with the cable toward the EGC holder (see Figure B-38). EGC Cable Figure B-38. EluGen Cartridge in Service Area 3.
  • Page 248 ICS-2000 Ion Chromatography System Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) Backpressure backpressure coil Coil (P/N 053765) in the ICS-2000 Ship Kit (P/N 064375). Connect one end of this coil to the EGC OUTLET Port port (see OUTLET Figure B-40).
  • Page 249 Setting Up the Eluent Generator Slide the EluGen cartridge down into the holder until it stops. Make sure the cable and backpressure coil remain out of the holder (see Figure B-41). NOTE To avoid crimping the lines inside the holder, do not twist the cartridge when you slide it into the holder.
  • Page 250 ICS-2000 Ion Chromatography System Connecting the Vent Line 1. Locate the vent line tubing kit (P/N 063092) supplied with the EluGen cartridge. Slide the label onto one end of the tubing and then push the barbed luer lock onto the tubing. 2.
  • Page 251 Setting Up the Eluent Generator Recording the EluGen Cartridge Serial Number in Chromeleon The serial number is printed on the EluGen cartridge label. 1. Open the Chromeleon Server Configuration program (select Start > Programs (or All Programs) > Chromeleon > Server Configuration).
  • Page 252 ICS-2000 Ion Chromatography System Conditioning the EluGen Cartridge After completing the installation steps described in the previous sections, condition the EluGen cartridge for 30 minutes. 1. Set a temporary waste container, such as a beaker, on top of the ICS-2000 and direct the yellow backpressure coil from the EluGen cartridge port to the container.
  • Page 253 Setting Up the Eluent Generator 15. Disconnect the backpressure coil from the EluGen cartridge OUTLET port 16. Connect the line to the port (see OUTLET Figure B-46). Figure B-46. EGC Inlet and Outlet Connections 17. Turn over the EluGen cartridge. Check for bubbles; if necessary, shake and tap the EluGen cartridge to remove bubbles.
  • Page 254 ICS-2000 Ion Chromatography System Deionized water in from pump TO EGC TO CR-TC ELUENT IN (red) TO DEGAS ELUENT IN TO CR-TC DEGAS REGEN OUT TO DEGAS (blue) ELUENT OUT CR-TC Eluent TO CR-TC/EPM DEGAS ELUENT OUT ELUENT OUT (yellow) WASTE, 10-32 to SEPARATOR...
  • Page 255 Setting Up the Eluent Generator 6. Turn on the pump and set the flow to the rate recommended for your application. 7. Pump deionized water through the CR-TC for at least 10 minutes. 8. Turn off the pump and remove the coupler. Completing the Eluent Generator Setup 1.

  • Page 256: Eluent Generator Setup For Carbonate/Bicarbonate Eluent

    ICS-2000 Ion Chromatography System B.14.2 Eluent Generator Setup for Carbonate/Bicarbonate Eluent Follow the instructions in this section if you are installing a K cartridge, an EPM, and an EGC CO Mixer (for generating carbonate/bicarbonate eluent). • If you are installing a K cartridge and an EGC CO Mixer (for generating carbonate eluent), go to...
  • Page 257 Setting Up the Eluent Generator Items Required to Complete the Setup Procedure The following items are required to setup the EluGen cartridge, EPM, and EGC CO Mixer. Except as noted, all items are supplied in the ICS-2000 Ship Kit (P/N 064375). Item Used To EPM mounting bracket...
  • Page 258 ICS-2000 Ion Chromatography System Figure B-49 illustrates the EluGen cartridge and EPM after installation. EluGen Cartridge (EGC EGC Holder Figure B-49. ICS-2000 with an EluGen Cartridge and EPM Installed B-54 Doc. 031857-03 4/06...
  • Page 259 Setting Up the Eluent Generator Figure B-50 illustrates the liquid flow path through the eluent generator components after all setup steps are complete and the eluent generator is ready for operation. EGC VENT EGC II K CO Send to waste Inlet Outlet (red...
  • Page 260 ICS-2000 Ion Chromatography System Installing the EluGen Cartridge 1. Tilt the cartridge holder to view the bottom and brace the holder against the back corner of the ICS-2000 (see Figure B-51). Figure B-51. Cartridge Holder (tilted to view the bottom) B-56 Doc.
  • Page 261 Setting Up the Eluent Generator 2. Use the precut tubing assemblies included in the ICS-2000 Ship Kit to extend the length of three lines as described below: a. Disconnect the line (red label) from the TO CR-TC ELUENT IN coupler that connects it to the line (yellow TO CR-TC ELUENT OUT label).
  • Page 262 ICS-2000 Ion Chromatography System Pull the line (orange label) out of the opening on TO CR-TC REGEN IN the side of the holder. Thread the three extended lines (red, yellow, and blue labels) through the opening on the side of the holder (see Figure B-53).
  • Page 263 Setting Up the Eluent Generator 7. Set the EluGen cartridge in the service area on top of the ICS-2000 and orient it with the cable toward the EGC holder (see Figure B-55). EGC Cable Figure B-55. EluGen Cartridge in Service Area 8.
  • Page 264 ICS-2000 Ion Chromatography System 11. Turn the EluGen cartridge over (with the fittings facing down). Shake the EluGen cartridge vigorously, and tap it with the palm of your hand 10 to 15 times. Check that all bubbles trapped in the eluent generation chamber are dislodged.
  • Page 265 Setting Up the Eluent Generator 13. Remove the plug from connector on EGC-1 top of the ICS-2000. 14. Orient the cartridge’s blue 4-pin electrical cable connector as shown in Figure B-58. Push it onto the EGC-1 connector on the ICS- 2000.
  • Page 266 ICS-2000 Ion Chromatography System 4. Screw the luer adapter Luer Adapter onto the vent opening. Luer Lock Remove the cap on the luer adapter by twisting. Save the EGC Vent Line cap. 5. Twist the luer lock and EluGen cartridge vent line into the luer adapter (see Figure...
  • Page 267 Setting Up the Eluent Generator 3. Lift the EluGen cartridge up slightly from the EGC holder and slide the EPM EGC Cable bracket onto the side of the holder (see Figure B-62). Figure B-62. EPM and Mounting Bracket Installed on EGC Holder 4.
  • Page 268 ICS-2000 Ion Chromatography System EGC VENT EGC II K CO Send to waste 35 cm (14 in) of black 30 cm (12 in) of black Inlet Outlet 0.25-mm (0.010-in) ID 0.25-mm (0.010-in) ID PEEK tubing (P/N 064340) PEEK tubing (P/N 064334) (red label) (yellow...
  • Page 269 Setting Up the Eluent Generator Recording the EluGen Cartridge and EPM Serial Numbers in Chromeleon The serial numbers are printed on labels on the EluGen cartridge and EPM. 1. Open the Server Configuration program (select Start > Programs (or All Programs) > Chromeleon > Server Configuration). 2.
  • Page 270 ICS-2000 Ion Chromatography System Plumbing the EluGen Cartridge and EPM for Conditioning Figure B-65 illustrates the liquid flow path through the EluGen cartridge and EPM during the conditioning procedure. EGC VENT EGC II K CO Send to waste Inlet Outlet (red label) TO CR-TC/EPM...
  • Page 271 Setting Up the Eluent Generator 1. Disconnect the line from port TO INJ VALVE IN - P/ELUENT OUT on the injection valve. 2. Use a 10-32 to 10-32 coupler (P/N 042627) to connect the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) to the line.
  • Page 272 ICS-2000 Ion Chromatography System Conditioning the EPM and EluGen Cartridge After completing the plumbing connections described in the previous sections, follow the steps below to hydrate the EPM for 5 minutes and then condition the EPM and EluGen cartridge for 30 minutes. 1.
  • Page 273 Setting Up the Eluent Generator 12. Turn off the EGC-1 and EGC-2 current. 13. Turn off the pump. Installing the EGC CO Mixer Figure B-67 illustrates the EGC CO Mixer after installation. TO EGC CO3 MIXER OUT (P/N 064343) TO EGC CO3 MIXER IN (P/N 064346) Figure B-67.
  • Page 274 ICS-2000 Ion Chromatography System 1. Use a Phillips head screwdriver to remove the existing small diameter mounting clips from the inside of the ICS-2000 door. 2. Install the larger diameter clips (P/N 063642) (see Figure B-68). Mixer Mounting Clips Figure B-68. Location of Mounting Clips for the EGC CO3 Mixer 3.
  • Page 275 Setting Up the Eluent Generator Filling the EGC CO Mixer with Deionized Water 1. Verify that the EGC-1 and EGC-2 currents are off. 2. Disconnect the EluGen cartridge inlet line (labeled ) from the coupler that connects it to the green pump PUMP/DAMPER outlet line (see Figure...
  • Page 276 ICS-2000 Ion Chromatography System 3. Connect the pump outlet to the mixer inlet (see Figure B-71). Pump Outlet Mixer Inlet Figure B-71. Pump Outlet Connected to Mixer Inlet for Mixer Hydration 4. Direct the mixer outlet line to a temporary waste container, such as a beaker.
  • Page 277 Setting Up the Eluent Generator Filling the EGC CO Mixer with the Carbonate/Bicarbonate Eluent Before initial use, follow the steps in this section to fill the EGC CO Mixer with the carbonate/bicarbonate eluent concentration required for the application to be run. Figure B-72 illustrates the liquid flow path through the eluent generator components during initial filling of the EGC CO...
  • Page 278 ICS-2000 Ion Chromatography System 1. Disconnect the pump outlet from the mixer inlet. 2. Reconnect the pump outlet and the EluGen cartridge inlet. 3. Connect a 10-32 to 10-32 coupler to the mixer inlet. 4. Disconnect the outlet of the yellow PEEK backpressure coil from the line and then connect the backpressure coil TO SRS/AES REGEN OUT to the mixer inlet (see...
  • Page 279 Setting Up the Eluent Generator To avoid damaging the suppressor, always turn off the suppressor before filling the mixer. The pump flow is on during filling, however, no flow reaches the suppressor. 7. Verify that the option is not Automatically turn on with pump selected on the ICS-2000 touch screen page (see SUPPRESSOR...
  • Page 280 ICS-2000 Ion Chromatography System Completing the Eluent Generator Setup for Carbonate/Bicarbonate Eluent Figure B-74 illustrates the liquid flow path through the ICS-2000 after all setup steps are complete and the eluent generator is ready for operation. To the pump Degas In EGC Vent EGC II Deionized...
  • Page 281 Setting Up the Eluent Generator 1. Connect the line to the coupler TO INJ VALVE IN - P/ELUENT OUT attached to the mixer inlet line. Route this line along the back of the drip tray to prevent it from being pinched when the ICS-2000 door is opened and closed 2.

  • Page 282: Eluent Generator Setup For Carbonate Eluent

    ICS-2000 Ion Chromatography System B.14.3 Eluent Generator Setup for Carbonate Eluent Follow the instructions in this section if you are installing a K cartridge and an EGC CO Mixer (for generating carbonate eluent). • If you are installing a KOH, LiOH, MSA, or NaOH EluGen cartridge and a CR-TC, go to Section B.14.1.
  • Page 283 Setting Up the Eluent Generator Items Required to Complete the Setup Procedure In addition to the EluGen cartridge and the EGC CO Mixer, you will need the following items to complete the eluent generator setup. Except as noted, all items are supplied in the ICS-2000 Ship Kit (P/N 064375). Item Use To Yellow 0.5 mL/min, 7 MPa...
  • Page 284 ICS-2000 Ion Chromatography System Figure B-76 illustrates the liquid flow path through the eluent generator components after all setup steps are complete and the eluent generator is ready for operation. EGC VENT EGC II K CO Send to waste Outlet Inlet TO DEGAS ELUENT IN...
  • Page 285 Setting Up the Eluent Generator Installing the EluGen Cartridge and Preparing for Conditioning Follow the steps in this section to install the EluGen cartridge and prepare it for conditioning before initial use. Figure B-77 illustrates the liquid flow path during the conditioning procedure.
  • Page 286 ICS-2000 Ion Chromatography System 1. Orient the EluGen cartridge with the fittings facing up and remove the plugs from the cartridge INLET OUTLET OUTLET fittings (see Figure B-54). INLET Figure B-78. EluGen Cartridge Fitting Plugs 2. Set the EluGen cartridge in the service area on top of the ICS-2000 and orient it with the cable toward the EGC holder (see Figure B-79).
  • Page 287 Setting Up the Eluent Generator Pull the coiled black lines out from inside the EGC IN EGC OUT EGC holder. Disconnect the line from the coupler that EGC IN connects it to the line. EGC OUT Connect the EGC IN line to the EluGen cartridge port...
  • Page 288 ICS-2000 Ion Chromatography System Slide the EluGen cartridge down into the holder until it stops (see Figure B-81). Make sure the cable remains out of the holder. NOTE To avoid crimping the lines, do not twist the cartridge when you slide it into the holder.
  • Page 289 Setting Up the Eluent Generator 10. Disconnect the line from port TO INJ VALVE IN - P/ELUENT OUT on the injection valve. 11. Use a 10-32 to 10-32 coupler (P/N 042627) to connect the TO INJ line to one end of the yellow 0.5 mL/min, VALVE IN - P/ELUENT OUT 7 MPa (1000 psi) backpressure coil (P/N 053765) (see Figure...
  • Page 290 ICS-2000 Ion Chromatography System Installing the EluGen Cartridge Vent Line 1. Locate the EluGen Cartridge Vent Line Tubing Kit (P/N 063092) supplied with the EluGen cartridge. 2. Slide the label onto one end of the tubing and then push the barbed luer lock onto the tubing.
  • Page 291 Setting Up the Eluent Generator 7. Direct the vent line to the waste container and insert it down into the gas separator waste tube about 10 to 15 cm (4 to 6 in). Recording the EluGen Cartridge Serial Number in Chromeleon The serial number is printed on the EluGen cartridge label.
  • Page 292 ICS-2000 Ion Chromatography System Conditioning the EluGen Cartridge After completing the installation steps described in the previous sections, condition the EluGen cartridge for 30 minutes before initial use. 1. Make sure the eluent reservoir is filled with ASTM filtered, Type I (18-megohm) deionized water.
  • Page 293 Setting Up the Eluent Generator Installing the EGC CO Mixer Figure B-67 illustrates the EGC CO Mixer after installation. TO EGC CO3 MIXER OUT (P/N 064343) TO EGC CO3 MIXER IN (P/N 064346) Figure B-87. ICS-2000 with an EGC CO Mixer Installed B-89 Doc.
  • Page 294 ICS-2000 Ion Chromatography System 1. Use a Phillips head screwdriver to remove the existing small diameter mounting clips from the inside of the ICS-2000 door. 2. Install the larger diameter mounting clips (P/N 063642) (see Figure B-88). Mixer Mounting Clips Figure B-88.
  • Page 295 Setting Up the Eluent Generator Filling the EGC CO Mixer with the Carbonate Eluent Before initial use, follow the steps in this section to fill the EGC CO Mixer with the carbonate eluent required for the application to be run. Figure B-90 illustrates the liquid flow path through the eluent generator components during initial filling of the EGC CO...
  • Page 296 ICS-2000 Ion Chromatography System 1. Connect a 10-32 to 10-32 coupler to the mixer inlet and connect the outlet of the yellow PEEK backpressure coil to the coupler. 2. Direct the mixer outlet to the temporary waste container. 3. Verify that the suppressor is off. To avoid damaging the suppressor and CR-TC, always turn off the suppressor before conditioning the EluGen cartridge.
  • Page 297 Setting Up the Eluent Generator Completing the Eluent Generator Setup for Carbonate Eluent Figure B-91 illustrates the liquid flow path through the ICS-2000 after all setup steps are complete and the eluent generator is ready for operation. To the pump Degas In EGC Vent EGC II Deionized...
  • Page 298 ICS-2000 Ion Chromatography System 1. Connect the line to the coupler TO INJ VALVE IN - P/ELUENT OUT attached to the mixer inlet line. Route this line along the back of the drip tray to prevent it from being pinched when the ICS-2000 door is opened and closed.

  • Page 299: Setting Up A Chromeleon Application

    Setting Up a Chromeleon Application B.15 Setting Up a Chromeleon Application Chromeleon provides application templates that let you quickly get started running. To use the application templates, you need to know the type of column and suppressor you will be using. This section shows how to create a sequence (a list of samples to be processed) and how to use an application template.
  • Page 300 ICS-2000 Ion Chromatography System 3. In the Browser, select File>New and select Sequence (using Wizard) (see Figure B-94). Figure B-94. Creating a New Sequence The Sequence Wizard Welcome page appears (see Figure Figure B-95. Sequence Wizard Welcome Page B-96 Doc. 031857-03 4/06...
  • Page 301 Setting Up a Chromeleon Application NOTE For details about a Sequence Wizard step, click the Help button on the Sequence Wizard page. 4. Click Next>. The Sequence Wizard Timebase page appears (see Figure B-96). Figure B-96. Example: Sequence Wizard Step 1 5.
  • Page 302 ICS-2000 Ion Chromatography System The Unknown Samples page appears (see Figure B-97). Figure B-97. Example: Sequence Wizard Step 2 6. Select Use Template from the drop-down list. 7. In the Template for Sample Name field, type Sample, click the right arrow, and select Sample Number #n.
  • Page 303 Setting Up a Chromeleon Application 10. Click Apply>>. Figure B-98 shows Sequence Wizard Step 2 after a sample is defined. Figure B-98. Example: Sequence Wizard Step 2 with Sample Defined 11. Click Next>. B-99 Doc. 031857-03 4/06...
  • Page 304 ICS-2000 Ion Chromatography System The Standard Samples page appears (see Figure B-99). Figure B-99. Example Sequence Wizard Step 3 12. Click Next> to omit this step and define standard injections later. Or, define one or more standard injections, and then click Next>. The Methods &...
  • Page 305 Setting Up a Chromeleon Application 13. Select the Use Application Template check box. 14. From the drop-down lists, select the type of suppressor to be installed and then select the column. In the example in Figure B-100, the 4-mm ASRS suppressor and AS17 column are selected.
  • Page 306 ICS-2000 Ion Chromatography System 17. Enter a name for the sequence and a location (see the example in Figure B-101). Figure B-101. Example: Sequence Wizard Step 5 18. Click Finish> and then click Done on the last page of the Sequence Wizard. B-102 Doc.
  • Page 307 Setting Up a Chromeleon Application The Browser appears, displaying the sequence you created (see Figure B-102). Notice the three files in the top pane of the Browser window. Figure B-102. Example Browser with Open Sequence • The .pgm file (program) contains commands and parameters for controlling the ICS-2000, controlling the autosampler (if used), and acquiring sample data.

  • Page 308: Verifying Chromeleon Communication

    ICS-2000 Ion Chromatography System 19. Double-click the .pan file to open the Control panel for your system (see the example in Figure B-103). Figure B-103. ICS-2000 System Control Panel Example NOTE If the Connected check box is not selected, click the box to connect the ICS-2000 to the Control panel.

  • Page 309: Equilibrating The System

    Equilibrating the System B.16 Equilibrating the System 1. After priming the pump, turn on the pump and set the flow to the operating rate. 2. Flush the system for about 5 minutes to equilibrate. 3. Monitor the system pressure reading from the Chromeleon Control panel. The EluGen cartridge requires at least 14 MPa (2000 psi) of system backpressure for removal of electrolysis gas from the eluent produced by the cartridge.

  • Page 310: Elugen Cartridge Backpressure Requirements

    ICS-2000 Ion Chromatography System B.16.1 EluGen Cartridge Backpressure Requirements The EluGen cartridge requires at least 14 MPa (2000 psi) of backpressure for optimal removal of electrolysis gas from the eluent produced by the cartridge. During system equilibration (see Section B.16), monitor the system pressure and verify that it is between 14 and 20 MPa (2000 and 3000 psi).

  • Page 311: Connecting An As Or As50 (Usb) Autosampler (Optional

    Connecting an AS or AS50 (USB) Autosampler (Optional) B.18 Connecting an AS or AS50 (USB) Autosampler (Optional) To use an AS or AS50 (USB) autosampler with the ICS-2000, the following requirements must be met: • The ICS-2000 injection valve must be linked to the AS or AS50 (USB) in the Chromeleon timebase (see Section B.18.2)

  • Page 312: As Or As50 (Usb) Connections

    ICS-2000 Ion Chromatography System NOTE The ICS-2000 and the AS or AS50 (USB) can be set up for stand-alone (front-panel) operation. This requires a TTL cable connection between the AS or AS50 (USB) and the ICS-2000. See Section D.3 for stand-alone setup instructions.
  • Page 313 Connecting an AS or AS50 (USB) Autosampler (Optional) 4. Connect the needle seal line from the autosampler to Autosampler port on the injection S (5) Needle Seal valve (see Figure B-105). Line The green waste line that is connected to port W (6) Waste the injection valve (see...
  • Page 314 ICS-2000 Ion Chromatography System B.18.2 AS or AS50 (USB) Chromeleon Setup 1. If you have not already done so, add the autosampler to the Chromeleon timebase (see Section B.8). 2. In the ICS-2000 Server Configuration properties, verify that the injection valve is controlled by the autosampler. To do this: a.
  • Page 315 Connecting an AS40 Automated Sampler (Optional) B.19 Connecting an AS40 Automated Sampler (Optional) 1. Place the AS40 to the left AS40 of the ICS-2000 on the Sample workbench. Outlet Line 2. Open the front door of the ICS-2000 and thread the outlet line from the AS40 through the left side slot at the front of the ICS-...
  • Page 316 ICS-2000 Ion Chromatography System NOTE If is not available, you can connect to RELAY OUT 1 RELAY OUT 2 or to one of the TTL outputs. For RELAY , connect the red wire to position 3 and the green OUT 2 wire to position 4.
  • Page 317 Connecting the Analog Output (Optional) the ICS-2000 injection valve are included by default in the program file (see Figure B-111). Relay 1 turned on to start Relay 1 turned off after 138 seconds the AS40 Load cycle and load the sample loop Injection valve Injection valve switched to Inject...
  • Page 318 ICS-2000 Ion Chromatography System B.21.1 Connect the Gas Source 1. Locate the barbed fitting (P/N 030071), pipe thread reducer (P/N 030087), 3-mm (1/8-in) ID tubing (P/N 040793), and ICS-2000 air regulator accessory (P/N 060054). These parts must be ordered separately. 2.
  • Page 319 Pressurizing the Eluent Reservoir (Optional) B.21.2 Pressurize the Eluent Reservoir NOTE Do not pressurize the ICS-2000 until after the reservoir is plumbed. 1. Pull out the air regulator knob and turn it fully counterclockwise to ensure there is no pressure to the reservoir when the gas source is turned on.
  • Page 320 ICS-2000 Ion Chromatography System B.22 Pump Continuous Seal Wash Connections (Optional) The pump includes a seal wash assembly that can be set up to continuously rinse the back of the piston seals to remove salt crystals and prolong the life of the seals. Follow the steps below to set up this feature.
  • Page 321 Pump Continuous Seal Wash Connections (Optional) b. Push the 152-cm (60-in) piece of clear tubing onto the drain tube on the top of the primary pump head. This is the waste line. Route this waste line through the lower tubing chase and out the back of the ICS-2000. c.
  • Page 322 ICS-2000 Ion Chromatography System B.23 Manually Connecting to a Control Panel This section describes how to manually connect the ICS-2000 to a Control panel if you do not use the application templates described in Section B.15. 1. In the Browser, expand the Dionex Templates\Panels\Dionex_IC\ICS-2000 System Panels folder.
  • Page 323 Manually Connecting to a Control Panel 3. To connect the Control panel to the ICS-2000, open the Control menu and click the name of the ICS-2000 timebase at the bottom of the menu. The Control panel is now connected to the ICS-2000 and the controls are enabled (see Figure B-116).
  • Page 324 ICS-2000 Ion Chromatography System B.24 Installation Troubleshooting Problem: When the ICS-2000 power is turned on for the first time, a Windows message box appears asking for a USB configuration file (cmwdmusb.inf). Possible Cause: The USB cable was connected and the power turned on before Chromeleon was installed.
  • Page 325 C • Touch Screen Operation The LCD touch screen on the ICS-2000 front panel allows local control of most ICS-2000 functions. This chapter describes how to operate the ICS-2000 from the touch screen. When the ICS-2000 starts up, the touch screen displays a startup page. This is followed by the page, which displays version and serial numbers INFORMATION...
  • Page 326 ICS-2000 Ion Chromatography System • Edit fields and command buttons have blue text on a lightly shaded background. Fields that display status information are not shaded. • Edit fields have square corners while command buttons are rounded. • To edit a field or select a command button, touch and release the field or button with your fingertip.
  • Page 327 C • Touch Screen Operation • Touching the page name (for example, ) in the bottom right corner opens a menu of pages (see Figure C-3). Touch a page name to display the page. Figure C-3. ICS-2000 Touch Screen Menu of Pages •...
  • Page 328 ICS-2000 Ion Chromatography System • Touching returns you to the page. HOME • Touching opens a list of options (see Figure C-5). Touch an option to select it. Note: To cancel and retain the current selection, touch outside of the list. Figure C-5.
  • Page 329 C • Touch Screen Operation C.3 Overview of ICS-2000 Touch Screen Pages Figure C-6 illustrates the organization of the ICS-2000 touch screen pages. HOME These pages can be PLOT opened from the menu or the Home page STATUS PUMP Diagnostic Pages Calibration Pages EGC1 FLOW RATE...
  • Page 330 ICS-2000 Ion Chromatography System C.4 Home Page Status Field Edit Field Command Button (selected) Command Button (unselected) Figure C-7. ICS-2000 Touch Screen Home Page C.4.1 Home Page Pump Controls Pressure Display page indicates the current pressure reading from HOME the pressure transducer. The pressure unit can be set to psi (the factory default), MPa, or bar (see Section C.7.1).
  • Page 331 C • Touch Screen Operation 2.00 mL/min. Refer to the column manual for the flow rate to use with your column and application. Column manuals are included on the Dionex Reference Library CD-ROM (P/N 053891). Pump On/Off Control Touching the button immediately starts the pump flow at the rate displayed in field.
  • Page 332 ICS-2000 Ion Chromatography System C.4.2 Home Page EGC Controls controls indicate the EGC-1 Eluent EGC-1 type and the eluent Cartridge Concentration concentration of each installed Type EluGen Cartridge (EGC). The EGC-2 field indicates whether CR-TC EGC-2 Eluent Cartridge Concentration the Continuously Regenerated Type Trap Column is on or off.
  • Page 333 C • Touch Screen Operation C.4.4 Home Page Injection Valve Controls buttons select the position of the Inject Load injection valve. A dark background and white text on a button indicates the current valve position. For details about the valve positions, see Section 2.4.5.
  • Page 334 ICS-2000 Ion Chromatography System Suppressor Controls Suppressor controls indicate the suppressor current setting and the type of suppressor installed. To set the suppressor type, change the current setting, or turn the suppressor on or off, touch the suppressor button. page appears (see Section C.9).
  • Page 335 C • Touch Screen Operation C.5 Plot Page page provides a real-time plot of the detector conductivity output. To PLOT open the page, touch the button on the page, or select from the PLOT HOME PLOT menu of pages. The page can display up to 60 minutes of data. Data older than 60 minutes is discarded.
  • Page 336 ICS-2000 Ion Chromatography System C.6 Status Page page displays the current status of various operating parameters. To STATUS open this page, touch the button on the page or select from STATUS HOME STATUS the menu of pages. Figure C-9. Status Page Status Page Controls •...
  • Page 337 C • Touch Screen Operation • The fields to the right of the bottle display the status of four other operating parameters. You can choose from eight possible parameters to display here. Figure C-9 shows the default parameters: Eluent time Flow rate EGC conc .
  • Page 338 ICS-2000 Ion Chromatography System C.6.1 Viewing Other Status Parameters Follow the steps below to view the status of a parameter that is not currently displayed on the page. STATUS 1. Touch the button. A drop-down list replaces the label for each Setup field, and the Setup...
  • Page 339 C • Touch Screen Operation 2. Touch the down arrow next to the field you want to change. A list of the available status parameters appears (see Figure C-12). Figure C-12. Status Page Parameter List 3. Touch the parameter you want to display. 4.
  • Page 340 ICS-2000 Ion Chromatography System C.7 Pump Page Use the page to set parameters related to pump operation, including PUMP pressure limits, pressure unit, vacuum degas pump settings, and eluent valve control. To open this page, select from the menu of pages. The current PUMP system pressure is displayed at the top of the screen.
  • Page 341 C • Touch Screen Operation • To change the low pressure limit, touch the field and enter the desired limit on the number pad. To select the pressure unit for all touch screen pages that display pressure values, touch the pressure unit field and select the desired unit on the list , or ) (see Figure...
  • Page 342 ICS-2000 Ion Chromatography System : The degas pump is always off. Always Off Always On : The degas pump is always on. This setting is reserved for test purposes by a Dionex Service Representative. Select the Degas Mode, Always On option only when testing the vacuum degas assembly.
  • Page 343 C • Touch Screen Operation C.8 Eluent Generator Page Use the page to set the eluent concentration of each ELUENT GENERATOR installed EluGen cartridge (EGC). You can also enter the serial number and view the expiration date and remaining life of each cartridge. This page also provides on/off control of a Continuously Regenerated Trap Column (CR-TC).
  • Page 344 ICS-2000 Ion Chromatography System If the ICS-2000 is not under Chromeleon control, enter the cartridge serial number by touching the Serial Number field on the ELUENT GENERATOR page (see Figure C-16) and entering the serial number on the keypad. If you are reinstalling a cartridge that was used previously, touch the down arrow instead of the number field.
  • Page 345 C • Touch Screen Operation C.8.3 Controlling the Eluent Generator Power • To manually control the eluent generator power, touch the EGC Off buttons on the page. ELUENT GENERATOR • To automatically control the eluent generator power simultaneously with the pump flow, select Auto turn on with pump NOTE The eluent generator power always turns off when the pump is off, regardless of whether the...
  • Page 346 ICS-2000 Ion Chromatography System C.8.5 Controlling the CR-TC Power • To manually control the CR-TC power, touch the CR-TC Off buttons on the page (see Figure C-17). ELUENT GENERATOR • To automatically control the CR-TC power simultaneously with the pump flow, select Auto turn on with pump NOTE The CR-TC power always turns off when the pump is off, regardless of whether the “Automatically turn...
  • Page 347 C • Touch Screen Operation C.9 Suppressor Page Use the page to specify the type of suppressor installed in the ICS- SUPPRESSOR 2000, turn the suppressor on and off, and select the amount of current supplied to the suppressor. To open this page, touch the suppressor button on the page, HOME or select...
  • Page 348 ICS-2000 Ion Chromatography System NOTE When Chromeleon is used to control the ICS-2000, the Program Wizard automatically calculates the current required for the application. • To manually control the suppressor power, touch the buttons. • To automatically control the suppressor power simultaneously with the pump flow, select Automatically turn on with pump NOTE The suppressor always turns off when the pump is off,...
  • Page 349 C • Touch Screen Operation C.10.1 Setting the Data Rise Time The data rise time determines the amount of filtering performed on the conductivity data. The rise time is a measure of how quickly the detector responds to a change in signal, and is defined as the time it takes the output signal to rise from 10% of its final value to 90% of its final value.
  • Page 350 ICS-2000 Ion Chromatography System C.10.3 Setting Analog Out Options The analog output connector on the ICS-2000 rear panel outputs an analog voltage signal proportional to the conductivity measured by the cell. The output is filtered using the selected data rise time (see Section C.10.1).
  • Page 351 C • Touch Screen Operation C.11 Information Page page displays the version numbers of the ICS-2000 control INFORMATION programs (Moduleware and Boot Block), the serial number of the ICS-2000, and the options that are installed in the ICS-2000. The page is INFORMATION displayed at start-up.
  • Page 352 ICS-2000 Ion Chromatography System C.12 Module Setup Page page lets you select options for the touch screen display and MODULE SETUP conductivity readings. To open this page, select from the menu of pages. SETUP Figure C-21. Module Setup Page Module Setup Options •...
  • Page 353 C • Touch Screen Operation C.13 Input/Output Page page provides control of the ICS-2000 TTL inputs and TTL INPUT/OUTPUT and relay outputs. To open this page, select from the menu of INPUT/OUTPUT pages. For details about relay and TTL control, see Appendix D. Figure C-22.
  • Page 354 ICS-2000 Ion Chromatography System C.14 Diagnostic and Calibration Pages Diagnostic and calibration functions can be performed either from the touch screen diagnostic and calibration pages or from the Chromeleon Wellness Panel. To open a touch screen diagnostic or calibration page, select from DIAGNOSTIC the menu of pages.
  • Page 355 D • TTL and Relay Control D.1 TTL and Relay Connections A 12-pin connector strip for TTL/relay control is located on the ICS-2000 rear panel. The connector provides two relay outputs, two TTL outputs, and four TTL inputs (see Figure D-1).
  • Page 356 ICS-2000 Ion Chromatography System The outputs can be used to control functions in external devices such as an autosampler or another Dionex module. When connected to a controlling device, the inputs can be programmed to perform the following ICS-2000 functions: •...
  • Page 357 D • TTL and Relay Control c. Connect the wires from the ICS-2000 connector plug to the TTL or relay connector pins on the other module(s). Additional connector plugs are provided with other Dionex modules. NOTE Check the polarity of each connection. Connect signal wires to signal (+) pins and ground wires to ground (-) pins.
  • Page 358 ICS-2000 Ion Chromatography System To select TTL input functions and the control type from the touch screen, go to the touch screen page (see Figure D-4). INPUT/OUTPUT Select a Function Select a Control Type Figure D-4. Input/Output Page: Selecting TTL Functions TTL Input Control Types The ICS-2000 TTL inputs respond to four types of signals to accommodate different controlling devices.
  • Page 359 D • TTL and Relay Control The action of the positive (rising) edge depends on the function: For function, the rising edge switches the injection valve Load/Inject to the Inject position. For the function, the rising edge turns Pump On off the pump (and suppressor).
  • Page 360 ICS-2000 Ion Chromatography System The TTL outputs are normally at 5 volts. Setting a TTL output to 0 volts turns on the action in the connected device. The TTL and relay output states can be controlled from Chromeleon, either by issuing direct control commands from the Chromeleon Control panel or by including the commands in a control program.
  • Page 361 D • TTL and Relay Control D.3 Example Setup for Stand-Alone Operation This section describes an example setup for stand-alone operation (operation without Chromeleon software) with an AS or AS50 (USB) autosampler and a third-party analog-to-digital converter and controller. If your configuration includes different devices, use the following steps as guidelines and refer to your product documentation for specific connection details.
  • Page 362 ICS-2000 Ion Chromatography System ICS-2000 Rear Panel Connections AS50 Rear Panel Connections ANALOG OUTPUT TTL 1 IN (+) TTL 2 OUT (+) TTL 2 IN (+) TTL 1 OUT (+) TTL 3 IN (+) RLY 2 OUT TTL 4 IN (+) RELAY RLY 1 OUT IN/OUT (-)
  • Page 363 D • TTL and Relay Control Assign ICS-2000 Input Functions Go to the ICS-2000 touch screen page and verify that the Inject INPUT/OUTPUT function is assigned to TTL input 1, that Autozero is assigned to TTL input 2, and that the TTL Input Control Type Normal Edge (see...
  • Page 364 ICS-2000 Ion Chromatography System You can now issue commands from the AS or AS50 (USB) to switch the ICS- 2000 injection valve position. Connect the Analog Output 1. Locate the analog cable for the third-party analog-to-digital converter and controller. 2. Connect the analog input connector on the third-party controller to the connector on the ICS-2000 rear panel (see Figure D-7).
  • Page 365 D • TTL and Relay Control Create an AS or AS50 (USB) Method and Schedule Figure D-11 is an example of AS or AS50 (USB) method timed events that can be used with the TTL/Relay connections shown in Figure D-7. TIMED EVENTS METHOD: TIME...
  • Page 366 ICS-2000 Ion Chromatography System D-12 Doc. 031857-03 4/06...
  • Page 367 E • Reordering Information Part Number Item Reservoirs 046548 2-L plastic reservoir assembly (includes stopper and cap) 039164 4-L plastic reservoir assembly (includes stopper and cap) Pump 057937 Primary pump head assembly 057938 Secondary pump head assembly 045721 Outlet check valve assembly, 10-32 045722 Inlet check valve assembly, 1/4-28 045994...
  • Page 368 ICS-2000 Ion Chromatography System Part Number Item Sample Loop and Injection Valve 042857 25-µL sample loop 057968 Injection valve 024305 Luer adapter fitting, 1/4-28 (for manual injections) 016388 1-cc syringe (for manual injections) 057896 Injection valve rebuild kit Suppressors, Cell, and Column Heater 056116 AAES Anion Atlas Electrolytic Suppressor 056118...
  • Page 369 F • FAQ How do I hook up an autosampler? For instructions on how to connect the ICS-2000 to an AS or AS50 autosampler, Section B.18 of this manual. To connect an AS40, see Section B.19. Also refer to the autosampler operator’s manuals, which are included on the Dionex Reference Library CD-ROM (P/N 053891).
  • Page 370 ICS-2000 Ion Chromatography System When should I replace the eluent generator cartridge? The ICS-2000 monitors EluGen cartridge use and displays a message when it is time to replace the cartridge. The message appears on the front panel screen and in the Chromeleon Audit Trail.
  • Page 371 F • FAQ F.12 How do I know when a column is dirty? See the troubleshooting section of the column manual. F.13 How do I clean a column? See the troubleshooting section of the column manual. F.14 Why is the conductivity high? Possible reasons for high conductivity include: •...
  • Page 372 ICS-2000 Ion Chromatography System Doc. 031857-03 4/06...
  • Page 373 G • Glossary Analytical Column Synonymous with Separator Column. Band Spreading The broadening of the sample band as it travels through the column. Band spreading can also occur in the injection valve, detector cell, and interconnecting tubing. Calibration Curve A graph showing detector response in peak height or area versus analyte concentration.
  • Page 374 ICS-2000 Ion Chromatography System Cell Constant (k) A factor determined experimentally by measuring the conductance (G) of a standard solution of known equivalent conductivity (k). κ G ⁄ The value of k depends upon the surface area of, and distance between, the electrode faces in the conductivity detector cell.
  • Page 375 G • Glossary of relatively clean sample. This allows large volumes of sample to be injected, lowering concentration detection limits. Conductivity A measure of the ease with which electrical current flows through a liquid contained between two opposite charged electrodes. Conductivity is a characteristic of ions in solution.
  • Page 376 ICS-2000 Ion Chromatography System Packing The material that fills a chromatographic column; usually a resin or silica-based material. Pellicular Resin A resin with a solid, nonporous core coated with a thin layer of more porous material. The exchange sites of pellicular ion exchange resins are located only on the surface layer of the bead.
  • Page 377 G • Glossary Siemens (S) Unit measure of conductance; the reciprocal of the electrical resistance of a solution. Suppressor A device used to minimize eluent conductivity and convert sample species to a common form, thus increasing detection sensitivity. Temperature Coefficient The percent of change in the conductivity of a solution with a 1 C change in temperature.
  • Page 378 ICS-2000 Ion Chromatography System Doc. 031857-03 4/06...
  • Page 379 Index Symbols Audit Trail, 2-16 Error messages, 4-1 % Offset (analog out), C-26 Icons, 4-1 Automated control, 2-12 Autosampler Loading samples with, 3-16 See also AS/AS50 (USB) Autosampler Adjusting the contrast, 2-2, C-1 See also AS40 Automated Sampler Air particulate samples, 3-13 Autozero, 2-19, 3-12, 3-19, C-9 Air regulator, B-114 –...
  • Page 380 ICS-2000 Ion Chromatography System Using the touch screen with Chromeleon, Cables Verifying communication, B-104 AS40 Relay Control, B-111 Wellness panel, 5-1 Power cord, 5-37 Chromeleon Server USB, B-10 Starting, 3-4, B-13 Calibration, 5-1 Chromeleon Server Configuration program Conductivity cell, 5-5 Timebase, B-18 Flow rate, 5-7 Column heater, 2-8, 2-42...
  • Page 381 Index Conductivity cell, 2-9, 2-43 Calibrating, 5-5 Daily maintenance, 3-21 Calibration constant, 5-26 Data High cell output, 4-21 Backing up, F-2 Leaking, 4-14 Deleting, F-2 No response, 4-20 Filtering, C-25 Replacement procedure, 5-25 Saving, 3-18 – 3-19 Setting the temperature, C-9 Data acquisition, 3-19 Specifications, A-3 Data analysis description, 1-3...
  • Page 382 ICS-2000 Ion Chromatography System Electrolytic pH modifer See EPM Electrolytic pH Modifier EGC, 2-6 Eluent Backpressure requirement, 2-38, 2-41 Aqueous, 3-22, 4-16 Concentration ranges, 2-36 Concentration, C-20 Concentration upper limits, 4-6, C-20 Degassing, 2-30 EGC-1 connector, 2-6, 5-44, 5-52, B-45, Delivery process, 1-2 B-61, B-84 Filtering, 3-6...
  • Page 383 Index Environmental specifications Filter, end-line Humidity, A-1 Bacterial contamination, 3-22, 4-16 Operating pressure, A-1 Dirty or clogged, 3-22, 4-15 Operating temperature, A-1 Installation, 3-6 EPM Electrolytic pH Modifier, 2-6, 2-38 – Filtering data, C-25 2-39 Fittings Normalized Life, 2-21 Leaking, 4-12 Replacement procedure, 5-64 Replacing, 5-14 Equilibration time, 3-12...
  • Page 384 ICS-2000 Ion Chromatography System Guard column, 2-8, B-23 Assigning devices to a timebase, B-18 Installation, B-24 Chromeleon, B-5, B-15 Columns, B-23 Computer, B-4 EGC vent line, B-37, B-53, B-61, B-79, B-86 Hardware not present error, 4-7 Eluent generator, B-35 Heater, column, 2-42 Eluent reservoir, B-29, B-115 See also Column heater Equilibrating the system, B-105...
  • Page 385 Index Key code (Chromeleon), B-6 Main power receptacle, 2-11 KOH EGC, 2-35 Maintenance, 3-21 Daily, 3-21 Ongoing, 3-21 Weekly, 3-22 Yearly, 3-22 LCD screen, 2-1 – 2-2, C-1 Manual sample processing, 3-2 Specifications, A-2 Methanesulfonic acid eluent, 2-35 See also Touch screen MicroMembrane Suppressor, 2-9 Leak sensor, 2-8 See also Suppressor...
  • Page 386 ICS-2000 Ion Chromatography System Operation pH modifer Equilibration time, 3-12 See EPM Electrolytic pH Modifier Overview, 3-1 Physical specifications Priming, 3-10, 5-36, B-34 Decibel level, A-1 Reservoir setup, 3-6 Dimensions, A-1 Sample preparation, 3-13 Weight, A-1 Sample processing, 3-2 Piston seals Setting operating conditions, 3-11 Replacement procedure, 5-18 –...
  • Page 387 Index Pressurizing Pump head waste valve Eluent reservoir, B-115 Leaking, 4-13 Prime button, C-7 O-ring replacement procedure, 5-23 Priming, 3-10, 5-36, B-31 – B-34 Pump heads, 5-16 Pump is out of prime, 4-15 Pump motor lost control alarm, 4-9 With a syringe, B-32 Pump page, C-16 With isopropyl alcohol, 5-36 Changing the pressure unit, C-16...
  • Page 388 ICS-2000 Ion Chromatography System Moving, F-1 Server Configuration program Troubleshooting, 4-20 AS50 configuration properties, B-21 Reverse peaks, C-25 Starting, 3-4, B-13 Rise time, C-25 Timebase, B-18 Run time, 2-15 Service chase, 2-6, 2-9 Running samples, 3-18 Service procedures, 5-17 See also Processing samples Check valve cleaning, 5-16 –...
  • Page 389 Index Stand-alone operation, D-7 – D-11 Cell heater, 2-43, C-9 Analog output connection, D-10 Column heater, 2-8, 2-42, C-8 AS/AS50 (USB) setup, D-9, D-11 Minimizing the effect of variations, 2-43 Example connections, D-8 Temperature compensation, 2-43, A-3 TTL and relay connections, D-7 Time, 2-15 Standards Timebase (Chromeleon), 3-5...
  • Page 390 ICS-2000 Ion Chromatography System Installation, B-120 Specifications, A-3 Liquid leaks, 4-12 Troubleshooting, 4-14 No cell response, 4-20 Valve Peak ghosting, 4-19 See Eluent valve Peak height, 4-20 See Injection valve Pump, 4-14 – 4-16 See Waste valve Retention time, 4-20 Version number, C-27 Selectivity, 4-20 Vacuum degas assembly, 4-23...

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