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Thermo Scientific
BIOLiner™
Instruction Manual
50119954-e • 07 / 2020
Visit us online to register your warranty:
thermofisher.com/labwarranty

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Table of Contents
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Summary of Contents for Thermo Scientific BIOLiner Multifuge X3

  • Page 1 Thermo Scientific BIOLiner™ Instruction Manual 50119954-e • 07 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
  • Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:...
  • Page 5: Table Of Contents

    Maintenance and Care ......................6-1 Cleaning Intervals .....................6-2 Cleaning ........................6-2 Disinfection ......................6-3 Decontamination .....................6-4 Autoclaving ......................6-5 Service of Thermo Fisher Scientific ................6-5 RCF-Values .........................A-1 RCF-Values BIOLiner bucket ................A-1 RCF-Values Microplate Carrier ................A-2 Chemical Compatibility Chart ..................B-1 Thermo Scientific 75003667 BIOLiner...
  • Page 7: Foreword

    • Do not use rotors which show any signs of corrosion and / or cracks. Do not touch the electronic components of the rotor and do not make any changes to the electronic or mechanical components. • Use only with rotors which have been loaded properly. • Never overload the rotor. Thermo Scientific 75003667 BIOLiner...
  • Page 8 ATTENTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. 75003667 BIOLiner Thermo Scientific...
  • Page 9 Rotor Specifications Contents “Technical Data” page 1-2 • Thermo Scientific 75003667 BIOLiner...
  • Page 10 55 / 55 Minimum temperature at n [°C] relating to <0 <0 <0 ambient temperature 23-25 °C Speed at 4 °C 3500 3500 3500 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK 75003667 BIOLiner Thermo Scientific...
  • Page 11 40 / 50 Minimum temperature at n [°C] relating to <0 <0 <0 ambient temperature 23-25 °C Speed at 4 °C 3500 3500 3500 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK Thermo Scientific 75003667 BIOLiner...
  • Page 12 70 / 60 70 / 60 Minimum temperature at n [°C] relating to ambient temperature 23-25 °C Speed at 4 °C 3500 3500 3500 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK 75003667 BIOLiner Thermo Scientific...
  • Page 13 50 / 50 50 / 50 Minimum temperature at n [°C] relating to ambient temperature 23-25 °C Speed at 4 °C 3500 3500 3500 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK Thermo Scientific 75003667 BIOLiner...
  • Page 14 50 / 55 Minimum temperature at n [°C] relating to <0 <0 <0 ambient temperature 23-25 °C Speed at 4 °C 3500 3500 3500 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK 75003667 BIOLiner Thermo Scientific...
  • Page 15 40 / 50 Minimum temperature at n [°C] relating to <0 <0 <0 ambient temperature 23-25 °C Speed at 4 °C 3500 3500 3500 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK Thermo Scientific 75003667 BIOLiner...
  • Page 16 70 / 60 70 / 60 Minimum temperature at n [°C] relating to ambient temperature 23-25 °C Speed at 4 °C 3500 3500 3500 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK 75003667 BIOLiner Thermo Scientific...
  • Page 17 50 / 50 50 / 50 Minimum temperature at n [°C] relating to ambient temperature 23-25 °C Speed at 4 °C 3500 3500 3500 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK Thermo Scientific 75003667 BIOLiner...
  • Page 18 70 / 60 70 / 60 Minimum temperature at n [°C] relating to ambient temperature 23-25 °C Speed at 4 °C 3500 3500 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK 75003667 BIOLiner Thermo Scientific 1-10...
  • Page 19 17.9 / 14.6 17.9 / 14.6 Pitch angle [°] Accel. / braking time [s] 50 / 50 50 / 50 50 / 50 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK Thermo Scientific 75003667 BIOLiner 1-11...
  • Page 20 Rotor Specifications Technical Data Table 1-10. 230 V, 50 / 60 Hz, Rectangular buckets 75003668 Thermo Scientific SL 40 Thermo Scientific SL 40 F Centrifuge Catalog # 75004512 75004542 Weight empty [kg] Max. cycle number 55000 55000 Maximum permissible load [g]...
  • Page 21 Rotor Specifications Technical Data Table 1-11. 230 V, 50 / 60 Hz, Microplate carriers 75003670 Thermo Scientific SL 40 Thermo Scientific SL 40 F Centrifuge Catalog # 75004512 75004542 Weight empty [kg] Max. cycle number 90000 90000 Maximum permissible load [g]...
  • Page 22 Rotor Specifications Technical Data Table 1-12. 120 V, 60 Hz, Rectangular buckets 75003668 Thermo Scientific SL 40 Centrifuge Catalog # 75004513 Weight empty [kg] Max. cycle number 55000 Maximum permissible load [g] 4 x 750 Maximum speed n [rpm] 3500...
  • Page 23 Rotor Specifications Technical Data Table 1-13. 120 V, 60 Hz, Microplate carriers 75003670 Thermo Scientific SL 40 Centrifuge Catalog # 75004513 Weight empty [kg] Max. cycle number 90000 Maximum permissible load [g] 4 x 750 Maximum speed n [rpm] 3500...
  • Page 25 Accessories Contents “Rotor data” page 2-2 • “Accessories” page 2-2 • “Bottle 75003699” page 2-3 • Thermo Scientific 75003667 BIOLiner...
  • Page 26: Chapter 1 Rotor Specifications

    Accessories Thermo Scientific General Purpose Rotors BIOLiner High Capacity Rotor BIOLiner High Capacity Rotor Rotor Specifications Capacity (ml) 4 x 750 Tube Dimensions (mm) 98 x 124 Angle Maximum Speed (rpm) 3500 K Factor 13307 Net Weight (kg) RCF (x g)
  • Page 27: Chapter 2 Accessories

    Weigh the bottles opposite in order to avoid an imbalance. Refer to the rotor loading chapter. CAUTION The bottle 75003699 is a wearing product. Bottle with signs of wear are no longer to be used. Replace the bottles. Damaged bottles can cause damage to the rotor and centrifuge. Thermo Scientific 75003667 BIOLiner...
  • Page 29: Autolock

    ™ AutoLock Contents “Rotor Installation” page 3-2 • “Removing the Rotor” page 3-3 • Thermo Scientific 75003667 BIOLiner...
  • Page 30: Rotor Installation

    WARNING If the rotor cannot be properly locked in place after several attempts, then the ™ AutoLock is defective and you are not permitted to operate the rotor. Check for any damage to the rotor, damaged rotors must not be used. Keep the hub area clear of objects. 75003667 BIOLiner Thermo Scientific...
  • Page 31: Removing The Rotor

    CAUTION Rotors supplied with a lid for aerosol-tight applications come with a ™ mandril, which belongs to the AutoLock . Be sure not to place the lid onto this mandril to prevent it from being damaged. Thermo Scientific 75003667 BIOLiner...
  • Page 32 ™ AutoLock Aerosol-tight Rotors ™ Figure 3-3. AutoLock -lid for aerosol-tight rotors ™ WARNING Mind the AutoLock -mandril inside the lid. Do not touch. 75003667 BIOLiner Thermo Scientific...
  • Page 33: Rotor Loading

    Rotor Loading Contents “Before a Run” page 4-2 • “Proper Loading” page 4-2 • “Improper Loading” page 4-3 • “Maximum Loading” page 4-3 • “Cycle Counter” page 4-3 • Thermo Scientific 75003667 BIOLiner...
  • Page 34: Before A Run

    To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times. You can use two rectangular buckets or two round buckets or two microplate carriers. Make sure that the opposing receptacles are holding tubes of the same weight. 75003667 BIOLiner Thermo Scientific...
  • Page 35: Improper Loading

    WARNING Replace the rotor when the specified number of cycles is reached. Due to the mechanical load a rotor can break and thus damage the centrifuge. Replace the buckets when the specified number of cycles is reached. Thermo Scientific 75003667 BIOLiner...
  • Page 36 Rotor Loading Cycle Counter Service life examples Usage profile Maximum service life at 50000 cycles frequent use 11 years 20 runs / day 220 days / year 75003667 BIOLiner Thermo Scientific...
  • Page 37: Aerosol-Tight Applications

    Aerosol-tight Applications Contents “Basic Principles” page 5-2 • “Fill Level” page 5-2 • “Checking the aerosol Tightness” page 5-2 • Thermo Scientific 75003667 BIOLiner...
  • Page 38: Basic Principles

    The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks, scratches and embrittlement is extremely important! Aerosol-tight applications are not possible if the vessel tops are open. Aerosol-tightness requires the correct operation when filling the sample vessels and closing the rotor lid. 75003667 BIOLiner Thermo Scientific...
  • Page 39 2. Raise the latch. The cap can now be easily placed on the bucket. Figure 5-4. Bucket with open lid 3. Lower the latch to close the bucket aerosol-tight; be sure the latch clicks into place. Thermo Scientific 75003667 BIOLiner...
  • Page 40 Figure 5-5. Bucket with closed lid CAUTION If the latch is not flipped down, the caps could be damaged during centrifuging. If the latch has not clicked into place, the bucket is not aerosol-tight. Never raise the bucket at its latch. 75003667 BIOLiner Thermo Scientific...
  • Page 41: Maintenance And Care

    Maintenance and Care Contents “Cleaning Intervals” page 6-2 • “Cleaning” page 6-2 • “Disinfection” page 6-3 • “Decontamination” page 6-4 • “Autoclaving” page 6-5 • “Service of Thermo Fisher Scientific” page 6-5 • Thermo Scientific 75003667 BIOLiner...
  • Page 42: Cleaning Intervals

    • Store the aluminium parts at room temperature or in a cold-storage room with the cavities pointing down. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. 75003667 BIOLiner Thermo Scientific...
  • Page 43: Disinfection

    CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Thermo Scientific 75003667 BIOLiner...
  • Page 44: Decontamination

    For general radioactive decontamination use a solution of equal parts of 70 % ethanol, 10 % SDS and water. Disinfect the rotor and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. 75003667 BIOLiner Thermo Scientific...
  • Page 45: Autoclaving

    • the suitability of the set-up site; • the lid lock and the safety system; • the rotor; • the fixation of the rotor and the drive shaft. Thermo Fisher Scientific offers inspection and service contracts for this work. Thermo Scientific 75003667 BIOLiner...
  • Page 47: Rcf-Values

    1800 10.5 1900 10.5 2000 10.5 2100 10.5 2200 10.5 1082 2300 10.5 1183 2400 10.5 1288 2500 10.5 1398 2600 10.5 1512 2700 10.5 1630 2800 10.5 1753 2900 10.5 1880 3000 10.5 1057 2012 Thermo Scientific 75003667 BIOLiner...
  • Page 48: Rcf-Values Microplate Carrier

    17.9 1059 2400 10.5 17.9 1153 2500 10.5 17.9 1251 2600 10.5 17.9 1353 2700 10.5 17.9 1459 2800 10.5 17.9 1569 2900 10.5 17.9 1683 3000 10.5 17.9 1057 1801 3100 10.5 17.9 1128 1923 75003667 BIOLiner Thermo Scientific...
  • Page 49 RCF-Values Speed (rpm) Min. radius Max. radius RCF Rmin RCF Rmax 3200 10.5 17.9 1202 2049 3300 10.5 17.9 1278 2179 3400 10.5 17.9 1357 2313 3500 10.5 17.9 1438 2451 Thermo Scientific 75003667 BIOLiner...
  • Page 51: Chemical Compatibility Chart

    S S U U S U M U S U U S U U U M U M U U U S U U S U S Benzene U U - M M - S U U U U U U U - M S M - Benzyl Alcohol Thermo Scientific 75003667 BIOLiner...
  • Page 52 S S S S S S S S S S S S - S U S S S S S S S S M S M S Ethylene Glycol S U - S M - S S S U S U S S S U Ethylene Oxide Vapor 75003667 BIOLiner Thermo Scientific...
  • Page 53 S U U S M S U S S S S Mercaptoacetic Acid S S S U S S M S S S S S U S U M S S S S S S S M S M U Methyl Alcohol Thermo Scientific 75003667 BIOLiner...
  • Page 54 M S U U M M S S S M S M S U M U - Phosphoric Acid (conc.) M S S S - S S S S S S S S S S S S S S S S S S S Physiologic Media (Serum, Urine) 75003667 BIOLiner Thermo Scientific...
  • Page 55 S S U S S S M U S U U U U U U M U M U U U S U M S U S Xylene U U S S S S U S S S S S S S S S S S S S S S S U S S S Zinc Chloride Thermo Scientific 75003667 BIOLiner...
  • Page 56 Performance unknown; suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use. Because no organized chemical resistance data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots. 75003667 BIOLiner Thermo Scientific...
  • Page 57 Scope of Supply ..........iii Cleaning Intervals ........... 6-2 Service .............6-5 Cycle Counter ..........4-3 Decontamination ..........6-4 Disinfection ............ 6-3 Fill Level ............5-2 Foreword ............iii Improper Loading ........... 4-3 Maintenance and Care ........6-1 Thermo Scientific 75003667 BIOLiner...
  • Page 59 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/rotors © 2009-2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.

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