Thermo Scientific Heraeus Multifuge X3 F Instructions For Use Manual
Thermo Scientific Heraeus Multifuge X3 F Instructions For Use Manual

Thermo Scientific Heraeus Multifuge X3 F Instructions For Use Manual

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Thermo Scientific
Heraeus Multifuge X3 F
Heraeus Multifuge X3 FR
Instructions for Use
50117505-e • 03 / 2020
Visit us online to register your warranty:
thermofisher.com/labwarranty

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Newton Aluvanze
January 23, 2025

what is the tolerance of the centrifuge when calibrating

Summary of Contents for Thermo Scientific Heraeus Multifuge X3 F

  • Page 1 Thermo Scientific Heraeus Multifuge X3 F Heraeus Multifuge X3 FR Instructions for Use 50117505-e • 03 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
  • Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:...
  • Page 3: Table Of Contents

    Intended Use ..........................iii Accident Prevention ........................ iv Precautions ..........................iv Chapter 1 Introduction and Description ....................1-1 Characteristics of the Heraeus Multifuge X3 F / X3 FR ...........1-2 Technical Data ......................1-3 Directives, Standards and Guidelines ................1-4 Functions and Features ......................1-5 Mains Supply ......................1-5 Rotor Selection ......................1-6...
  • Page 4 Service of Thermo Fisher Scientific ................6-5 Chapter 7 Trouble Shooting ........................7-1 Mechanical Emergency Door Release ..............7-2 Troubleshooting by User ..................7-3 When to contact Customer Service ................7-5 Chemical Compatibility Chart ................A-1 Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 5: Preface

    Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty. Scope of Supply Article Number Quantity Check Centrifuge Heraeus Multifuge X3 F Power supply cable 50117505 Instruction manual 70009824 Anti-corrosion oil If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
  • Page 6: Accident Prevention

    Accident Prevention Accident Prevention Prerequisite for the safe operation of the Heraeus Multifuge X3 F / X3 FR is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user. The safety regulations contain the following basic recommendations: •...
  • Page 7 CENTRIFUGE AND LEAVE THE AREA IMMEDIATELY. Intended Use The centrifuge is to be used for separating materials of different density or particle size suspended in a liquid. Maximum sample density at maximum speed: 1,2 ---------- - Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 8 WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 9: Introduction And Description

    Introduction and Description Contents “Characteristics of the Heraeus Multifuge X3 F / X3 FR” page 1-2 • “Technical Data” page 1-3 • “Directives, Standards and Guidelines” page 1-4 • “Functions and Features” page 1-5 • “Mains Supply” page 1-5 •...
  • Page 10: Characteristics Of The Heraeus Multifuge X3 F / X3 Fr

    Characteristics of the Heraeus Multifuge X3 F / X3 FR Characteristics of the Heraeus Multifuge X3 F / X3 FR The Heraeus Multifuge X3 F / X3 FR is an in-vitro diagnostics device according to the In-Vitro Diagnostics Directive 98/79/EC.
  • Page 11: Technical Data

    Introduction and Description Technical Data Technical Data The technical data of the Heraeus Multifuge X3 F / X3 FR is listed in the following table. Table 1-1. Technical Data Heraeus Multifuge X3 F / X3 FR Feature Value Environmental conditions...
  • Page 12: Directives, Standards And Guidelines

    100V 50Hz IEC 61010-1 2 Edition IEC 61010-2-020 2 Edition IEC 61010-2-101 EN 61326-1 EN 61326-2-6 EN 55011A EN 61000-6-2 EN ISO 13485 Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 13: Functions And Features

    The following table contains an overview of the electrical connection data for the Heraeus Multifuge X3 F / X3 FR. This data is to be taken into consideration when selecting the mains connection socket. Table 1-4. Electrical connection data of the Heraeus Multifuge X3 F / X3 FR Mains...
  • Page 14: Rotor Selection

    Introduction and Description Rotor Selection Rotor Selection The Heraeus Multifuge X3 F / X3 FR is supplied without a rotor. Various rotors are available to choose from. TX-750 75003607 with round buckets 75003608 with rectangular buckets 75003614 incl. Microtiter carrier 75003617 incl.
  • Page 15 Contents “Before Setting up” page 2-2 • “Transporting the Centrifuge” page 2-4 • “Location” page 2-4 • “Aligning the Centrifuge” page 2-4 • “Mains Connection” page 2-5 • “Storage” page 2-6 • Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 16: Chapter 2 Before Use

    1. Place the pallet with the centrifuge so that you have at leased 1.5 m room in front of the pallet. 2. Release the rails from the pallet. 3. Screw the rails onto the pallet in front of the centrifuge. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 17 4. Remove the protectors that locate the centrifuge on the pallet. Release the castors. Use several people in order to roll the centrifuge off the pallet. 5. Fix the castors once the centrifuge is in its final position. Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 18: Transporting The Centrifuge

    The supporting structure must be suitable for horizontal setup of the centrifuge. CAUTION If the centrifuge isn’t level, imbalances can occur and the centrifuge can be damaged. Do not place anything under the feet to level the centrifuge. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 19: Mains Connection

    3. Check whether the cable complies with the safety standards of your country. 4. Make sure that the voltage and frequency correspond to the figures on the rating plate. 5. Establish the connection to the power supply with the connecting cable. Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 20: Storage

    • Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. • Avoid direct sunlight. • Fix the castors. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 21: Control Panel

    Control Panel Contents “Control Panel” page 3-2 • “Keys” page 3-2 • Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 22: Keys

    Acceleration Curves Use the Toggle key to change the display mode. (speed / RCF value, Changing the display sample / chamber temperature, run time counter from start or preset mode speed on) Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 23: Operation

    “Entering Parameters” page 4-4 • “Centrifugation” page 4-10 • “Temperature Adaptation during Standstill” page 4-11 • “Short-term Centrifugation” page 4-11 • “Removing the Rotor” page 4-12 • “Aligning the Centrifuge” page 4-13 • Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 24: Switch On Centrifuge

    Note The lid should audibly click into place. Rotor Installation The approved rotors for the Heraeus Multifuge X3 F / X3 FR are listed in section “Rotor Selection” page 1-6. Use only the rotors and accessories from this list in the centrifuge.
  • Page 25 Keep the centrifuge spindle area of the rotor clear of objects. CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it at its handle. Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 26: Entering Parameters

    Entering Parameters Acceleration curve The Heraeus Multifuge X3 F / X3 FR offers you a total of 9 acceleration and 10 braking curves with which samples and gradients can be centrifuged. After the centrifuge is turned on, the last running profile selected is shown.
  • Page 27 This can be accounted for in the calculation above if required. Running time preselection 1. Press the TIME key in order to open the runtime selection menu. The display shows the message "Set Time". Enter the desired runtime in hh.mm Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 28 Press the TOGGLE key to toggle between the two display modes. When AIR is lit, the temperature inside the rotor chamber will be controlled. This setting is recommended when using the centrifuges pretemp feature. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 29 The digits show in sequential order. 3. Confirm your entry by pressing ENTER Your entry will be automatically confirmed if you do not press any key for 5 seconds. 4. The display shows: The display shows: Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 30 When you use adaptors, you will have to specify the radius. For appropriate values please refer to the instruction manual for the rotor. 1. Press ENTER to confirm your selection and open the radius input menu. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 31 2. Press the program memory key to load any of the programs 1-5. Press the DIRECTORY key in order to load any of the programs 1-99 by means of the numeric pad. Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 32: Centrifugation

    You cannot open the lid as long as the centrifuge is running. Imbalance indicator If a load is imbalanced, this will be indicated at speed higher than approx. 300 rpm by the message "Imbalanced load". The run will terminate. 4-10 Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 33: Temperature Adaptation During Standstill

    Short-term Centrifugation For short-term centrifuging, the Heraeus Multifuge X3 F / X3 FR has a PULSE- function. By holding down the PULSE key , spinning will start and continue until the key is let go.
  • Page 34: Removing The Rotor

    . Be sure not to place the lid onto this mandrill to prevent it from being damaged. Figure 4-2. AutoLock -lid for aerosol-tight rotors WARNING Mind the AutoLock -mandrill inside the lid. Do not touch the mandrill. 4-12 Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 35: Aligning The Centrifuge

    Operation Aligning the Centrifuge Aligning the Centrifuge 1. To turn off the centrifuge put the mains switch to "0". Thermo Scientific Multifuge X3 F / X3 FR 4-13...
  • Page 36: Chapter 5 System Menu

    LCD powersave? ENTER STOP ____ Lid passcode? Set lid passcode ENTER Program passcodes? ENTER Auto lid open? ENTER Software ID: 0547V01 0547V01 ENTER 4641V05 Software NVRAM identification example display Cycle count: 2368 ENTER Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 37: Description

    Press ENTER key to confirm. Program passcodes Press 1 (yes) on the numeric pad if the centrifuge programs should be protected with a passcode. Otherwise press 0 (no). Press ENTER key to confirm. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 38 Press ENTER key to confirm. Software ID Here you fine the current software version. Press ENTER key to confirm. Cycle count Here you fine the current numbers of cycles. Press ENTER key to confirm. Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 39: Maintenance And Care

    Maintenance and Care Contents “Cleaning Intervalls” page 6-2 • “Cleaning” page 6-2 • “Disinfection” page 6-3 • “Decontamination” page 6-4 • “Autoclaving” page 6-5 • “Service of Thermo Fisher Scientific” page 6-5 • Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 40: Cleaning Intervalls

    • After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (70009824). Also treat the cavities with oil. • Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 41: Disinfection

    CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 42: Decontamination

    For general radioactive decontamination use a solution of equal parts of 70% ethanol, 10% SDS and water. 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 43: Autoclaving

    • the electrical equipment; • the suitability of the set-up site; • the lid lock and the safety system; • the rotor; • the fixation of the rotor and the drive shaft. Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 44 Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are performed for free during the warranty period and afterwards for a charge. This is only valid if the centrifuge has only been mainained by a Thermo Fisher Scientific service technician. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 45: Trouble Shooting

    Trouble Shooting Contents “Mechanical Emergency Door Release” page 7-2 • “Troubleshooting by User” page 7-3 • “When to contact Customer Service” page 7-5 • Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 46: Mechanical Emergency Door Release

    Emergency Door Release Figure 7-1. Emergency Door Release Note You need to pull both levers in order to unlock both locks. 4. Push the cord back into the centrifuge and mount the plug. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 47: Troubleshooting By User

    Motor overtemp. The centrifuge cannot be operated. Restart the centrifuge. If an error message appears again, inform Customer Service. The run does not start or the centrifuge runs down without being braked. Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 48 Time has expired for the lid lock drive while opening the lid. The run does not start or the centrifuge runs Restart the centrifuge. down without being braked. If an error message appears again, inform Customer Service. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 49: When To Contact Customer Service

    This information can be found on the back near the inlet for the power supply cable. To identify the software version, proceed as follows: 1. Hold down any of the keys and then switch on the centrifuge. You enter the system menu. 2. Press the START key Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 50 Trouble Shooting When to contact Customer Service 3. Press and hold the ENTER key , until the following message is displayed: Software ID: XXXXXXXX 4. Communicate the software version to the service technician. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 51: Chemical Compatibility Chart

    S S S S S S M S S S Benzene S S U U S U M U S U U S U U U M U M U U U S U U S U S Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 52 S S S M S S S U S M U Ethyl Alcohol (95%) Ethylene Dichloride U U - S M - U U S U U U U U U U - U S U - Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 53 U U U U U U U - Aqua Regia Solution 555 (20%) S S S - S S S S S S S - S S S - S S S S S S Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 54 Perchloric Acid (70%) Phenol (5%) U S U - S M M - S U M U U S U M S M S U U S U M M M S Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 55 S S S S S S S S S S S S S S S S S S S Triton X-100 Urea U S S S S - S S S M S S S S - S S S M S - Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 56 Chemical resistance data is included only as a guide to product use. No organized chemical resistance data exists for materials under the stress of centrifugation. When in doubt we recommend pretesting sample lots. Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 57 Removing the Rotor ........4-12 Failure message ..........7-3 Rotor Installation ..........4-2 Features ............1-5 Rotor Selection ..........1-6 Running time preselection .......4-5 Imbalance indicator ........4-10 Intended Use ............v Safety features ..........1-2 Thermo Scientific Multifuge X3 F / X3 FR...
  • Page 58 Starting centrifuge program ......4-10 Stopping the centrifugation program ..... 4-11 Storage ............2-6 Switch on Centrifuge ........4-2 Technical Data ..........1-3 Temperature Adaptation .......4-11 Transporting the Centrifuge ......2-4 Trouble Shooting ..........7-1 Multifuge X3 F / X3 FR Thermo Scientific...
  • Page 59 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/centrifuge © 2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Heraeus is a registered trademark of Heraeus Holding GmbH licensed to Thermo Fisher Scientific. Delrin, TEFLON, and Viton are registered trademarks of DuPont.

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