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5.F. Kinetic Measurements
The kinetic measurement feature provides a method for monitoring real-time luminescence. The data point frequency
is the rate at which the measurements are taken for a particular luminescent reaction. Access the kinetic measurement
options from the Protocol Screen (Figure 5). Touch Kinetics to turn Kinetics on. Use the up and down arrow keys to
set the frequency of data point collection as well as the number of total data points. The integration time does not apply
when the kinetics feature is turned on. Be sure to connect the luminometer to a computer or printer using the RS-232
serial cable to collect the data points during a kinetic protocol. Touch OK to save your changes.
5.G. Default Protocol
Select Default Protocol to measure your sample for an integration of one second. Touch Default Protocol to jump
directly to the Home Screen.
5.H. Running a User Protocol
To access a saved protocol, select Run User Protocol. The next screen will display protocols previously saved on the
luminometer. Identify and touch the name of your protocol. The next screen will display the parameters of the saved
protocol for your review and adjustment. Touch OK to go to the Home Screen.
6.
Measuring Your Samples
The GloMax
20/20 Luminometer is a bottom-reading luminometer and does not require a minimum sample volume.
®
The sample compartment of the luminometer accommodates 35mm petri dishes and 1.5ml microcentrifuge tubes with
a tube holder.
1.
If using a microcentrifuge tube, open the lid of the GloMax
Tube Holder.
2.
Place your sample in a microcentrifuge tube, and gently place the tube in the microcentrifuge tube holder. Be
sure that the microcentrifuge tube cap is open. Alternatively, you can place a petri dish directly into the sample
compartment.
3.
Close the lid.
4.
Optional: Touch Sample ID to name your sample. Using the keypad, enter the sample name into the name field
(Figure 6). You can enter a maximum of 10 characters. Touch Save to save the sample ID.
5.
Touch Measure Luminescence once to begin the protocol sequence. To abort a measurement in progress,
touch the screen once.
8
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM276 · Revised 5/21
20/20 Luminometer and insert the Microcentrifuge
®
www.promega.com
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