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5.D. Running a Promega Protocol
The GloMax
20/20 Luminometer accommodates the measurement requirements for luminescent assays from
®
Promega. Touch Run Promega Protocol from the Protocol Menu (Figure 4), and select the protocol that corresponds
to your assay system. For example, if you are using the Steady-Glo
Steady-Glo. The luminometer will open a Steady-Glo
automatically programmed. Touch OK to accept the parameters and return to the Home Screen. You are now ready to
measure your samples.
Figure 4. The Protocol Menu.
5.E. Creating a New Protocol
If you touch Create New Protocol, the GloMax
(Figure 5). The integration time is another term for the measurement duration. Touch Integration, then use the up
and down arrow keys to increase or decrease the integration time. When you are finished, touch Save As to save your
protocol under a specific name of your choice. Alternatively, touch OK to return to the Home Screen to measure your
samples. The luminometer will automatically name your protocol "New Protocol" and return to the Home Screen.
Figure 5. A typical parameters screen.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
www.promega.com
Assay for a luciferase gene reporter study, select
®
protocol with the recommended measurement settings
®
20/20 Luminometer prompts you to adjust the integration time
®
7
TM276 · Revised 5/21
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