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- Page 1 ® LOOXSTER Enrichment Kit Instructions for use english For research use only Analytik Jena AG | Life Science | Konrad-Zuse-Straße 1 | 07745 Jena / Deutschland | www.bio.analytik-jena.de...
- Page 2 850-203-001-0010 Manufacturer: Phone +49 (0) 36 41 / 77-94 00 Analytik Jena AG +49 (0) 36 41 / 77-76 77 76 Konrad-Zuse-Straße 1 www.bio.analytik-jena.com 07745 Jena/Germany lifescience@analytik-jena.com ® LOOXSTER Enrichment Kit 11/2013...
- Page 3 A 20T and should be stored at 2-8° C. All other components can be stored at room temperature (15-30° C). Under these conditions a shelf life of 6 months is guaranteed. ® Upon reconstitution LOOXSTER Magnetic Particles should be kept at 2-8° C. Under ® these conditions LOOXSTER Magnetic Particles retain their performance for at least 1 week.
- Page 4 P302+P352: IF ON SKIN: Wash with plenty of soap and water. Precaution ® • Please read the manual carefully prior to use LOOXSTER Enrichment Kit. In case of questions or unclarity, please contact us. • Do not use reagents after date of expiry.
- Page 5 Subsequently, the protein-DNA complex is solved in presence of an elution buffer and the enriched ® bacterial or fungal DNA can be harvested from the LOOXSTER -particles. After performing a final solid-phase extraction step the desalted and concentrated bacterial or fungal DNA can be applied to downstream analytical and preparative applications.
- Page 6 The LOOXSTER Enrichment Kit contains coated paramagnetic particles. Processing of paramagnetic particles requires a strong magnetic separator adapted to 1.5 ml and 2.0 ml reaction tubes. We recommend Magnetrack small; Analytik Jena, cat. No. 845- MG-2000002. ® For application of LOOXSTER Enrichment usual laboratory instrumentation is required.
- Page 7 ® LOOXSTER Enrichment Protocol ® Reconstitution of lyophilized LOOXSTER -Magnetic Particles ® One screwed cap tube contains lyophilized LOOXSTER Magnetic Particles for two ® separate LOOXSTER -applications. Reconstitution results in 400 µl of ready-to use ® ® LOOXSTER Magnetic Particles. If only one LOOXSTER enrichment application is ®...
- Page 8 ® Adjust DNA sample using Water W to a volume of 550 µl, pipet the sample into ® a Tube 2.0 ml TUB2 and add 550 µl of LOOXSTER Binding Buffer LBB. TUB2 ® Add 175 µl of reconstituted LOOXSTER Magnetic Particles LMP (carefully resuspended) and mix the preparation by inverting the tube several times.
- Page 9 Place Desalting Spin Column DSC into a Collection Tube CT and apply the diluted LOOXSTER eluate completely to the Desalting Spin Column DSC. Close lid and centrifuge Spin Column DSC for 30 sec at 13.000 – 16.000 x g.
- Page 10 Literature Chao Xu, Chuanbing Bian, Robert Lam, Aiping Dong & Jinrong Min. The structural basis for selective binding of non-methylated CpG islands by the CFP1 CXXC domain. Nat. Commun. 2011;2:227. ® LOOXSTER Enrichment Kit 11/2013...
- Page 11 (for bacterial and fungal DNA) Technical Support ® If you have any questions or experience any difficulties regarding LOOXSTER please do not hesitate to contact us for technical support. You can contact us by email, phone, fax or post: Analytik Jena AG Life Science Konrad-Zuse-Straße 1...
- Page 12 Keep away from heat or direct sunlight Vor Hitze und Sonneneinstrahl ung schützen Garder à l abri de la chaleur et de toute exposition lumineus e Non esporre ai raggi solare Mauténgase alejado del calor o la luz solar directa 203_MA_1_11/13_EN ® LOOXSTER Enrichment Kit 11/2013...
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