Shodex Silica C18M 4D Operation Manual

（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column adhesive label, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex Silica C18M series is a monomeric type ODS column
filled with high purity silica gel modified with octadecyl group.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F6650040 Silica C18M 4D
F6650041 Silica C18M 4E
Base Material : Spherical porous particles of silica modified with octadecyl group
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Methanol = 30/70

4. Usable Conditions

Product Name
Silica C18M 4D
Silica C18M 4E
Operation Manual

Shodex Silica C18M 4D, 4E
Column Size (mm)
I.D.
4.6
4.6
Recommended
Flow Rate (mL/min)
1.0
1.0
SHOWA DENKO K.K. (https://www.shodex.com/)
Particle
Size (μm)
Length
150
250
Maximum
pH
Pressure (MPa)
Range
(Per Column)
20 2 - 7.5
Theoretical Plate Number
(Per Column)
5 ≥ 10,000
5 ≥ 16,000
Temperature (°C)
Recommended
20 - 50
ver.210702E
Maximum
60
1