Shodex HILICpac VG-50 4D Operation Manual

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(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex HILICpak VG-50 series is a polymer-based HILIC
column modified with amino group. The column is suitable for the analysis of saccharides and sugar alcohols.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F7630200
HILICpak VG-50 4D
F7630100
HILICpak VG-50 4E
F6711100
HILICpak VG-50G 4A
Base Material
: Spherical porous particles of polyvinyl alcohol modified with amino group
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile = 20/80
Operation Manual
Shodex
HILICpak
Column Size (mm)
I.D.
4.6
4.6
4.6
SHOWA DENKO K.K. (https://www.shodex.com/)
VG-50 4D, 4E
Particle
Size (μm)
Length
150
5
250
5
10
5
ver.211105E
Theoretical Plate Number
(Per Column)
≥ 5,500
≥ 7,500
(Guard Column)
1

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Summary of Contents for Shodex HILICpac VG-50 4D

  • Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex HILICpak VG-50 series is a polymer-based HILIC column modified with amino group. The column is suitable for the analysis of saccharides and sugar alcohols.
  • Page 2 (4) When a sample contains protein or lipid, make sure to remove them prior to the injection. Presence of those substances may damage the column. Note · Use of guard column is recommended to protect the analytical column. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 3 This may result in creating an empty space in the column and deteriorates the column. Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 4 VG-50 4E 6 min 10 min 0.1 M HNO aq. solution 75 min 120 min 6 min 10 min 0.1 M NaOH aq. solution 75 min 120 min 12 min 20 min Eluent 40 min 60 min SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
  • Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.

This manual is also suitable for:

Hilicpac vg-50 4eHilicpac vg-50 4aF7630200F7630100F6711100

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