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Shodex R I-502 Operation Manual

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Ｏ ｐ ｅ ｒ ａ ｔ ｉ ｏ ｎ Ｍ ａ ｎ ｕ ａ ｌ
Ｏ ｐ ｅ ｒ ａ ｔ ｉ ｏ ｎ
Ｏ ｐ ｅ ｒ ａ ｔ ｉ ｏ ｎ
Ｏ ｐ ｅ ｒ ａ ｔ ｉ ｏ ｎ
Ｒｅｆｒａｃｔｉｖｅ Ｉｎｄｅｘ Ｄｅｔｅｃｔｏｒ
READ this operation manual CAREFULLY
READ
READ
READ
8
Shodex
RI-501 Refractive Index Detector.
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Ｍ ａ ｎ ｕ ａ ｌ
Ｍ ａ ｎ ｕ ａ ｌ
R
ＲＩ-５０２
CAREFULLY before using
CAREFULLY
CAREFULLY
ＳＴ１５－０００７７－C

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  • Page 1 Ｍ ａ ｎ ｕ ａ ｌ Ｍ ａ ｎ ｕ ａ ｌ Ｍ ａ ｎ ｕ ａ ｌ ＲＩ-５０２ Ｒｅｆｒａｃｔｉｖｅ Ｉｎｄｅｘ Ｄｅｔｅｃｔｏｒ READ READ READ READ this operation manual CAREFULLY CAREFULLY CAREFULLY before using CAREFULLY Shodex RI-501 Refractive Index Detector.
  • Page 2 Copyright 2015-2019, SHOKO SCIENC SHOKO SCIENC SHOKO SCIENCE E E E CO.,LTD SHOKO SCIENC CO.,LTD CO.,LTD Printed in Japan. CO.,LTD All rights reserved. Contents of this publication may not be reproduced in any form or by any means(including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from the copyright owner.
  • Page 3 Shodex RI-502 Refractive Index Detector or granting or implying any license or immunity under any patent or other rights. Shodex RI-502 Refractive Index Detector must be used on user's own responsibility and in strict compliance with all applicable laws and regulations.
  • Page 4 Precautions Precautions Precautions Precautions Thank you for your continued patronage. Observe the following precautions in order to make safe and stable use of the detector. 【 Important Important ! ! ! ! 】 Important Important Precautions are divided into three groups in this operation manual depending on the degree of danger.
  • Page 5 Do not use the detector with the cover open; always unplug the Warning Warning Warning Warning detector from the main power source before opening the cover. If the detector is used in a manner not specified by this operation Warning Warning Warning Warning...
  • Page 6 If the detector is not to be used for more than one week, then, Caution Caution Caution Caution prior to storage, wash the flow line with pure water or acetone, and dry the line by allowing nitrogen gas to flow through. Do not use any eluent which might corrode the material, such as Caution Caution...

  • Page 7: Table Of Contents

    Contents Contents Contents Contents １ ． Accessories １ １ １ １ ･･･････････････････････････････････ ２ ． Features ２ ２ ２ ２ ･･･････････････････････････････････････ ３ ． Specifications ３ ３ ３ ３ ･････････････････････････････････ ４ ． Detection Principle ４ ４ ４ ４ ････････････････････････････ ４－１ Optical System ４...
  • Page 8 1. 1. 1. 1. Accessories Accessories Accessories Accessories When unpacking, please check if all the following accessories are contained: Details of Details of Details of Details of Accessories Accessories Accessories Accessories 【 】 Name Name Name Name Specification Specification Specification Specification Quantity Quantity...
  • Page 9 2. Features 2. Features 2. Features 2. Features Shodex RI-502 is Deflection Type Refractive Index Detector with following features: 1) Stability system provides for a very fast start up time The double temperature control The double temperature control The double temperature control...
  • Page 10 3. Specifications 3. Specifications 3. Specifications 3. Specifications 1) Construction : Deflection type 2) Refractive Index Range : 1.00 ～ 1.75 3) Range : 2.5 ～ 5120 μRIU 4) Linearity : 6000 μRIU 5) Noise : 25 nRIU or less (Response : 1.5 seconds) 6) Drift : 2 μRIU/ｈ...
  • Page 11 4. Detection Principle 4. Detection Principle 4. Detection Principle 4. Detection Principle 4 4 4 4 - - - - 1 Optical System 1 Optical System 1 Optical System 1 Optical System In a refractive index detector of the deflection type, the light beam undergoes a deflection in proportion to a difference in refractive index between one liquid and the other as it passes through the two compartments of the flow cell, each being filled with a different liquid.
  • Page 12 When a difference develops in the refractive index between the sample and the Reference in the flow cell, the image on the photo sensor moves horizontally as illustrated in Fig. 4.3 below ( moves from (a) to (b) ). The electric signals emitted respectively from the photo sensor change in proportion to the extent of movement of the image.
  • Page 13 3 Electric Circuit 3 Electric Circuit 3 Electric Circuit The electrical circuit of Shodex RI-502 consists of two I/V converters, a signal processing circuit (with display and keyboard), a temperature control circuit and a light intensity control circuit. The temperature control circuit and light intensity control circuit are controlled by the signal processing circuit.
  • Page 14 Two photo sensing elements generate a photocurrent proportional to the amount of light. These currents are then converted to the voltage signal in the I/V converters. These voltage signals will then be sent to the signal processing circuit where their Balance will be precisely converted into the digital signal corresponding to the refractive index.

  • Page 15: ５－１ Front Panel

    □ □ □ □ □ □ Fig. 5.1 Front panel of Shodex RI-502 Name of parts Function Power Switch Press this key once to turn on or off the unit. □ A 24-digit 2-row character display panel. This is used for LCD Display □...

  • Page 16: ５－２ Side Panel

    5 5 5 5 - - - - 2 2 2 2 Side Panel Side Panel Side Panel Side Panel □ Fig. 5.2 Side panel of Shodex RI-502 Name of parts Function In case of internal eluent leak, the eluent will be discharged □...

  • Page 17: ５－３ Back Panel

    □ □ □ □ □ □ □ □ Fig. 5.3 Back panel of Shodex RI-502 Name of parts Function Communication port □ LAN communication port [COM] Zero in terminals When these two terminals are short-circuited just as when the zero □...

  • Page 18: ５－４ Display

    Power connector The included power cable should be plugged into □ [INPUT] this connector. These are the terminals to ground the main body Ground terminals of the detector. □ 5 5 5 5 - - - - 4 Displ 4 Display ay ay ay 4 Displ 4 Displ The display shows a “Monitor screen”...
  • Page 19 This indicates error or operation status. If there are multiple messages to be displayed, the priority message is displayed. ( 1 ) ROM : Is displayed when a ROM error has occurred. ( 2 ) RAM : Is displayed when a RAM error has occurred. ( 3 ) PARAMETER : Is displayed when a parameter memory error has occurred.
  • Page 20 Press The Enter key to start the SPAN CHECK. Press The S.Seq key to start and stop the S.SEQ. Press The Enter key to display the result of the validation Each S.SEQ mode is changed with the ENTER key and Arrow Keys The S.SEQ mode is displayed by a screen2.
  • Page 21 Table 5 Table 5- - - - 1 Settable Table 5 Table 5 1 Settable 1 Settable parameters 1 Settable parameters parameters parameters Parameter Selectable value Unit Default 2.5，5，10，20，40，80，160， REC. RANGE 320，640，1280，2560，5120 μRIU/10mV 5120 （12 Steps） INTEG. RANGE 1280，5120 μRIU/1024mV 5120 TEMPERATURE OFF，30～55 （1 Step）...

  • Page 22: ５－５ Start-Up Sequence

    ５－５ ５－５ Start Start- - - - Up Sequence Up Sequence ５－５ ５－５ Start Start Up Sequence Up Sequence 1）S.SEQ MODE Choose one of the built-in sequences (Fine, Standard, Coarse) from the menu or make own sequence (Custom). Each S.SEQ mode is changed with the ENTER key and Arrow Keys Table 5 Table 5- - - - 2 2 2 2 Parameters (Start Parameters (Start- - - - Up Sequence)

  • Page 23: ６－１ Power Connection And Grounding

    6 6 6 6 ..I I I I n n n n stallation stallation and Co and Con n n n ne ne ne nec c c c t t t t i i i i on on on on stallation stallation and Co...

  • Page 24: ６－２ Signal Cable Connections

    6 6 6 6 - - - - 2 Signal cable Connections 2 Signal cable Connections 2 Signal cable Connections 2 Signal cable Connections Make correct connections using the provided signal cables in accordance with the figures below. Connect red wire to ”+” terminal, white wire to”-” terminal and clear wire to “FG”. ) driver ) driver Inserts the end of lead wire while...

  • Page 25: ６－３ Tube Connections

    6 6 6 6 - - - - 3 Tube Connections 3 Tube Connections 3 Tube Connections 3 Tube Connections When connecting this detector to a high performance liquid chromatograph through tubes, wash the chromatograph and columns thoroughly with the eluent in use and make sure that the eluent is clean before connecting the tube.

  • Page 26: ６－４ Operation Procedures

    6 6 6 6 - - - - 4 Operation Procedures 4 Operation Procedures 4 Operation Procedures 4 Operation Procedures Use the detector in accordance with the following procedures: [ Automatic Start up ] [ Automatic Start up ] [ Automatic Start up ] [ Automatic Start up ] 1) Set parameters on the operating parameter-setting screen.
  • Page 27 If any eluent freezes inside the detector, the flow line might break Caution Caution Caution Caution damaging the detector. If there is any chance of the eluent freezing during stoppage or storage of the detector, withdraw the eluent completely from the flow line of the detector. When using any eluent containing a salt of high concentration, Caution Caution...

  • Page 28: ７－１ Flow Line Cleaning

    7 7 7 7 ..Care Care And Maintenance And Maintenance Care Care And Maintenance And Maintenance 7 7 7 7 - - - - 1 1 1 1 Flow Line Cleaning Flow Line Cleaning Flow Line Cleaning Flow Line Cleaning Depends on the solvents in use, the cleaning procedure is varied.
  • Page 29 It is recommended that the HPLC system be validated from time to time to maintain the accuracy and reliability of analysis. Thin task of validation has been made easier due to the unique and user-friendly feature of Shodex RI-502. Press the key to obtain the “SPAN VALIDATION”...
  • Page 30 2) Equilibrate Shodex RI-502 by pumping deionized water through the reference and sample cells. Use the same deionized water as that used to prepare the sucrose standard solution. Start pumping at a flow rate of 1mL/min and do the start-up sequence.
  • Page 31 9 9 9 9 ..Troubleshoot Troubleshooti i i i ng ng ng ng Troubleshoot Troubleshoot If any abnormality occurs, try to solve the problem in accordance with the Instructions below. Should the problem not be corrected even after taking the corrective actions below, contact the agent from whom you purchased the detector.
  • Page 32 Problem Possible cause Solution Large baseline drift 1. Insufficient solvent 1. Replace the solvent thoroughly. replacement. (Refer to the notes in 6-4.) 2. Room temperature 2. Use detector in places where changes fluctuates greatly. in room temperature are small. 3. Bubbles are present in 3.
  • Page 33 Please note that our instrument is for industrial / professional use only. Please contact your Shodex office or Shodex distributor when Please contact your Shodex office or Shodex distributor when...
  • Page 34 Appendix Appendix 1 1 1 1 Appendix Appendix P P P P r r r r oper oper oper opert t t t y y y y Data Data Data Data of Solvents of Solvents of Solvents of Solvents Flash Boiling Fire Point Polarity...
  • Page 35 Appendix 2 2 2 2 Appendix Appendix Appendix Shodex RI RI RI RI- - - - 5 5 5 5 0 0 0 0 2 2 2 2 Components and Subassemblies Components and Subassemblies Components and Subassemblies Components and Subassemblies...
  • Page 36 Yi Road, Jing An, Shanghai, 200041, P.R.China Tel : +1 212 370 0033 Tel : +86 (0)21 6217-6111 Fax : +1 212 370 4566 Fax : +86 (0)21 6217-9879 E-mail : sales@shodexchina.com E-mail : support@shodex.net URL:http://www.shodex.net/ URL :http://www.shodex.com/index_ch.html [EUROPE [EUROPE & AFRICA [EUROPE [EUROPE &...

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