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Operation Manual Shodex Asahipak NH2P-50 10E, NH2P-90 20F, NH2P-130G 7B
Shodex™ Asahipak™ NH2P-50 10E,
NH2P-90 20F, NH2P-130G 7B
Europe, Middle East, Africa, Russia
www.shodex.de
Operation Manual
Sales Office:
Showa Denko Europe GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich
Germany
Version 150305E
Technical Support:
info@shodex.de
+49 (0)89 / 93 99 62 37
Quotes and Orders:
order@shodex.de
+49 (0)89 / 93 99 62 34
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Table of Contents
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Summary of Contents for Shodex Asahipak NH2P-50 10E
- Page 1 Operation Manual Shodex Asahipak NH2P-50 10E, NH2P-90 20F, NH2P-130G 7B Version 150305E Operation Manual Shodex™ Asahipak™ NH2P-50 10E, NH2P-90 20F, NH2P-130G 7B Sales Office: Technical Support: Showa Denko Europe GmbH info@shodex.de Shodex Business +49 (0)89 / 93 99 62 37...
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Page 2: Handling Instructions
(Please read this manual carefully before using the column to ensure performance and life.) Introduction The Shodex Asahipak NH2P series is packed with a polymeric gel containing chemically bonded polyamine groups. Under the chromatographic conditions generally employed for the silica-base amino columns, the NH2P column provides equal resolution and far greater reproducibility. -
Page 3: System Clean Up
Operation Manual Shodex Asahipak NH2P-50 10E, NH2P-90 20F, NH2P-130G 7B Version 150305E Attention! High-temperature operation may result in the generation of an air bubble, necessitating degassing. Low-temperature operation may require reduced flow rates, because of increased eluent viscosity. System Clean Up Clean up the LC system including the injector and the sample loop by switching the valve and flow the eluent before column installation. -
Page 4: Sample Preparation
When replacing buffer solution to acetonitrile solution, also flow 100% water to the column before flowing acetonitrile. Attention! Shodex line filter, Asahipak NH2P-LF (F6710100), is a filter for removing undesired contaminants in the eluant. By installing the filter between the pump and the injector, deterioration of the column is reduced. -
Page 5: Column Cleaning
5) When the column is not used for two weeks or more, replace the in-column solvent with the shipping solvent, remove it from the LC system, close each end with a stopper, and store it at controlled room temperature. 6) Contact Shodex website (www.shodex.de) or Shodex partners regarding product and analysis applications. - Page 6 User’s exclusive remedy and Showa Denko K.K.‘s exclusive liability for such claim are limited to the replacement of the Shodex Column in question. In no event is Showa Denko K.K. liable for any indirect, incidental or consequential damage arising out of in connection with the Shodex Column, whether or not such damage is allegedly based on breach of warranty, negligence or otherwise.
- Page 7 Version 150305E Product Information: Shodex HILIC Columns Shodex HILIC columns are polymer-based (polyvinyl alcohol particles) for better stability (pH 2-13). This results in a longer life time of the columns than silica-based columns. They can be used in a temperature range from 4 to 60°C. The HILIC columns are suitable for RI (isocratic), evaporative light scattering, corona charged aerosol and LC-MS/MS detectors (gradient mode).
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