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(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex CLNpak EV series is GPC clean-up columns packed
with styrene divinylbenzene copolymers. It is suitable for clean-up of residual pesticide components in foods.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Code
F6090003
CLNpak EV-2000 AC
CLNpak EV-G AC
F6090004
Base Material
: Spherical porous particles of styrene divinylbenzene copolymer
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Acetone/Cyclohexane = 3/7
4. Usable Conditions
Product Name
CLNpak EV-2000 AC
CLNpak EV-G AC
Usable solvents are listed below.
(1) The standard eluent is a mixture of acetone and cyclohexane.
Recommended acetone to cyclohexane ratio is between 2:8 and 3:7.
(2) Up to 100 % (v/v) acetone, ethyl acetate, cyclohexane, toluene, and dichloromethane are usable.
Operation Manual
Shodex CLNpak EV-2000 AC
Column Size (mm)
Product Name
Recommended Flow Rate
(mL/min)
4 - 5
-
Resonac Corporation (https://www.shodex.com/)
Particle Size
I.D.
Length
20.0
300
20.0
100
Maximum Pressure
(MPa/column)
2.5
-
ver.20221202E
Theoretical Plate
Number
(μm)
(Per Column)
16
≥ 4,000
16
(Guard Column)
Recommended
Temperature (°C)
40
1
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Summary of Contents for Shodex CLNpak EV-2000 AC
- Page 1 Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number. 1. Introduction Thank you for purchasing the Shodex product. Shodex CLNpak EV series is GPC clean-up columns packed with styrene divinylbenzene copolymers. It is suitable for clean-up of residual pesticide components in foods.
- Page 2 · When a sample is dissolved in a solvent other than the eluent and if the sample matrix contains components which do not dissolve in the eluent fully, precipitates may form and clog the column. Note · Use of a guard column is recommended to protect the analytical column. Resonac Corporation (https://www.shodex.com/)
- Page 3 100 % new solvent. (3) When replacing the current solvent with a solvent with low miscibility/solubility to the current solvent, first use a solvent that is miscible/soluble to both solvents, and then replace it with the new solvent. Resonac Corporation (https://www.shodex.com/)
- Page 4 · Never allow inside the column to dry. It can damage the column. 9. Column Inspection Please refer to the inspection method described in the CoA. At Shodex, “half width method” is adopted for the calculation of plate count and “asymmetry factor (Fas)” is adopted for the calculation of peak symmetry. Please refer to the Shodex website for the detail: https://www.shodex.com/en/da/07.html...
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