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TROUBLE SHOOTING GUIDE
Please use this guide to troubleshoot any problems that may arise.
For further assistance, please contact the technical support staff, toll free, at
1-800-776-4431.
Problem
Clogged Column
Low DNA yield
Problems in
downstream
applications
Cause
Carry-over of debris.
Sample too viscous.
Incomplete disruption of
starting material.
Poor lysis of sample
DNA remains bound to
column
DNA washed off
Salt carry-over
Ethanol carry-over
Plant DNA Purification Kit
Suggestion
Following extraction with
chloro:isoamyl alcohol, make
sure no particulate material is
transferred.
Do not exceed suggested
amount of starting material.
Alternatively, increase
amounts of Buffers CTB and
BB and use two or more
columns per sample.
For both dry and fresh
samples, obtain a fine
homogeneous powder before
adding CTB Buffer or use a
Bead Ruptor if possible.
Decrease amount of starting
material or increase amount
of CTB Buffer, chloro:isoamyl
alcohol, and BB Buffer.
Increase elution total volume
to 200 μL and incubate
at 65oC for 5 min before
centrifugation.
Dilute DW Buffer by adding
appropriate volume of absolute
ethanol prior to use.
DW Buffer must be at room
temperature.
Following the second wash
spin, ensure that the column is
dried by centrifuging 2 min at
maximum speed.
7
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